|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | High-resolution multimodal flexible coherent Raman endoscope显示文摘Coherent Raman scattering microscopy is a fast,label-free,and chemically specific imaging technique that shows high potential for future in vivo optical histology.However,the imaging depth in tissues is limited to the sub-millimeter range because of absorption and scattering.Realization of coherent Raman imaging using a fiber endoscope system is a crucial step towards imaging deep inside living tissues and providing information that is inaccessible with current microscopy tools.Until now,the development of coherent Raman endoscopy has been hampered by several issues,mainly related to the fiber delivery of the excitation pulses and signal collection.Here,we present a flexible,compact,coherent Raman,and multimodal nonlinear endoscope(4.2mm outer diameter,71mm rigid length)based on a resonantly scanned hollow-core Kagomé-lattice double-clad fiber.The fiber design enables distortion-less,background-free delivery of femtosecond excitation pulses and back-collection of nonlinear signals through the same fiber.Sub-micrometer spatial resolution over a large field of view is obtained by combination of a miniature objective lens with a silica microsphere lens inserted into the fiber core.We demonstrate high-resolution,high-contrast coherent anti-Stokes Raman scattering,and second harmonic generation endoscopic imaging of biological tissues over a field of view of 320μm at a rate of 0.8 frames per second.These results pave the way for intraoperative label-free imaging applied to real-time histopathology diagnosis and surgery guidance. | Alberto Lombardini Vasyl Mytskaniuk Siddharth Sivankutty Esben Ravn Andresen Xueqin Chen Jérôme Wenger Marc Fabert Nicolas Joly Frédéric Louradour Alexandre Kudlinski Hervé Rigneault | 2018 | Light(Science & Applications)2018，7，1： | 6 |
| 2 | Mechanical Properties of Dielectric Thin Films显示文摘 | Mahodaux C Giovannini H | 1997 | SPIE1997，3133，： | 1 |
| 3 | Specific docking of apolipoprotein A-I at the cell surface requires a functional ABCA1 transporter显示文摘 | Chambenoit O Hamon Y Marguet D Rigneault H Rosseneu M Chimini G | | 0，，： | 1 |
| 4 | Modal analysis of spontaneous emission in a planar microcavity显示文摘 | Rigneault H M onneret S | 1996 | Phys Rev1996，54，3： | 1 |
| 5 | Enhancement of single-molecule fluorescence detection in subwavelength apertures 显示文摘 | RIGNEAULT H CAPOULADE J DINTINGER J | 2005 | Physical Review Letters2005，95，11： | 1 |
| 6 | Dynamic multiple target tracing to probe spatiotemporal cartography of cell membranes显示文摘 | SERGE A BERTAUX N RIGNEAULT H | 2008 | Nature Methods2008，5，8： | 1 |
| 7 | An adaptive microscope for the imaging of biological surfaces显示文摘Scanning fluorescence microscopes are now able to image large biological samples at high spatial and temporal resolution.This comes at the expense of an increased light dose which is detrimental to fluorophore stability and cell physiology.To highly reduce the light dose,we designed an adaptive scanning fluorescence microscope with a scanning scheme optimized for the unsupervised imaging of cell sheets,which underly the shape of many embryos and organs.The surface of the tissue is first delineated from the acquisition of a very small subset(~0.1%)of sample space,using a robust estimation strategy.Two alternative scanning strategies are then proposed to image the tissue with an improved photon budget,without loss in resolution.The first strategy consists in scanning only a thin shell around the estimated surface of interest,allowing high reduction of light dose when the tissue is curved.The second strategy applies when structures of interest lie at the cell periphery(e.g.adherens junctions).An iterative approach is then used to propagate scanning along cell contours.We demonstrate the benefit of our approach imaging live epithelia from Drosophila melanogaster.On the examples shown,both approaches yield more than a 20-fold reduction in light dose-and up to more than 80-fold-compared to a full scan of the volume.These smart-scanning strategies can be easily implemented on most scanning fluorescent imaging modality.The dramatic reduction in light exposure of the sample should allow prolonged imaging of the live processes under investigation. | Faris Abouakil Huicheng Meng Marie-Anne Burcklen Herve Rigneault Frederic Galland Loïc LeGoff | 2021 | Light(Science & Applications)2021，10，11： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员