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| 1 | A dual positional specific lipoxygenase functions in the generation of flavor compounds during climacteric ripening of apple显示文摘Lipoxygenase(LOX)is an important contributor to the formation of aroma-active C6 aldehydes in apple(Malus×domestica)fruit upon tissue disruption but little is known about its role in autonomously produced aroma volatiles from intact tissue.We explored the expression of 22 putative LOX genes in apple throughout ripening,but only six LOXs were expressed in a ripening-dependent manner.Recombinant LOX1:Md:1a,LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b proteins showed 13/9-LOX,9-LOX,13/9-LOX and 13-LOX activity with linoleic acid,respectively.While products of LOX1:Md:1c and LOX2:Md:2b were S-configured,LOX1:Md:1a and LOX2:Md:2a formed 13(R)-hydroperoxides as major products.Site-directed mutagenesis of Gly567 to an alanine converted the dual positional specific LOX1:Md:1a to an enzyme with a high specificity for 9(S)-hydroperoxide formation.The high expression level of the corresponding MdLOX1a gene in stored apple fruit,the genetic association with a quantitative trait locus for fruit ester and the remarkable agreement in regio-and stereoselectivity of the LOX1:Md:1a reaction with the overall LOX activity found in mature apple fruits,suggest a major physiological function of LOX1:Md:1a during climacteric ripening of apples.While LOX1:Md:1c,LOX2:Md:2a and LOX2:Md:2b may contribute to aldehyde production in immature fruit upon cell disruption our results furnish additional evidence that LOX1:Md:1a probably regulates the availability of precursors for ester production in intact fruit tissue. | Doreen Schiller Carolina Contreras Jörg Vogt Frank Dunemann Bruno G Defilippi Randolph Beaudry Wilfried Schwab | 2015 | Horticulture Research2015，2，1： | 6 |
| 2 | Determination of firmness and sugar content of apples using near-infrared diffuse reflectance显示文摘 | Lu Renfu Daniel E Guyer Randolph M Beaudry | 2000 | Journal Texture Studies2000，31，： | 1 |
| 3 | Determination of firmness and sugar content of apples using near-infrared diffuse reflectance显示文摘 | Renfu Lu Daniel E Guyer Randolph M Beaudry | 2000 | Journal of Texture Studies2000，31，： | 1 |
| 4 | Determination of sugar contant and firmnesss of apple using NIR diffuse reflectance显示文摘 | Lu RenFu Guyer Daniel E Beaudry Randolph | 2000 | Journal of Texture Studies2000，31，6： | 1 |
| 5 | Depletion of 1-MCP by ‘non-target' materials from fruit storage facilities显示文摘 | VALLEJO Fernando BEAUDRY Randolph | 2006 | Postharvest Biology and Technology2006，40，2： | 1 |
| 6 | Lipoxygenase-associated apple volatiles and their relationship with aroma perception during ripening显示文摘 | Carolina Contreras Randolph Beaudry | 2013 | Postharvest Biology and Technology2013，，： | 1 |
| 7 | Effect of non-conventional atmospheres and bio-based packaging on the quality and safety of Listeria monocytogenes -inoculated fresh-cut celery ( Apium graveolens L.) during storage显示文摘 | Jaime González-Buesa Natalie Page Chelsea Kaminski Elliot T. Ryser Randolph Beaudry Eva Almenar | 2014 | Postharvest Biology and Technology2014，，： | 1 |
| 8 | Uncovering genetic and metabolite markers associated with resistance against anthracnose fruit rot in northern highbush blueberry显示文摘Anthracnose fruit rot(AFR),caused by the fungal pathogen Colletotrichum fioriniae,is among the most destructive and widespread fruit disease of blueberry,impacting both yield and overall fruit quality.Blueberry cultivars have highly variable resistance against AFR.To date,this pathogen is largely controlled by applying various fungicides;thus,a more cost-effective and environmentally conscious solution for AFR is needed.Here we report three quantitative trait loci associated with AFR resistance in northern highbush blueberry(Vaccinium corymbosum).Candidate genes within these genomic regions are associated with the biosynthesis of flavonoids(e.g.anthocyanins)and resistance against pathogens.Furthermore,we examined gene expression changes in fruits following inoculation with Colletotrichum in a resistant cultivar,which revealed an enrichment of significantly differentially expressed genes associated with certain specialized metabolic pathways(e.g.flavonol biosynthesis)and pathogen resistance.Using non-targeted metabolite profiling,we identified a flavonol glycoside with properties consistent with a quercetin rhamnoside as a compound exhibiting significant abundance differences among the most resistant and susceptible individuals from the genetic mapping population.Further analysis revealed that this compound exhibits significant abundance differences among the most resistant and susceptible individuals when analyzed as two groups.However,individuals within each group displayed considerable overlapping variation in this compound,suggesting that its abundance may only be partially associated with resistance against C.fioriniae.These findings should serve as a powerful resource that will enable breeding programs to more easily develop new cultivars with superior resistance to AFR and as the basis of future research studies. | MacKenzie Jacobs Samantha Thompson Adrian E.Platts Melanie J.A.Body Alexys Kelsey Amanda Saad Patrick Abeli Scott J.Teresi Anthony Schilmiller Randolph Beaudry Mitchell J.Feldmann Steven J.Knapp Guo-qing Song Timothy Miles Patrick P.Edger | 2023 | Horticulture Research2023，10，10： | 0 |
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