|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Tenofovir vs lamivudine plus adefovir in chronic hepatitis B:TENOSIMP-B study显示文摘AIM To demonstrate the non-inferiority(15% non-inferiority limit) of monotherapy with tenofovir disoproxil fumarate(TDF) vs the combination of lamivudine(LAM) plus adefovir dipivoxil(ADV) in the maintenance of virologic response in patients with chronic hepatitis B(CHB) and prior failure with LAM.METHODS This study was a Phase IV prospective, randomized, open, controlled study with 2 parallel groups(TDF and LAM+ADV) of adult patients with hepatitis B e antigen(HBe Ag)-negative CHB, prior failure with LAM, on treatment with LAM+ADV for at least 6 mo, without prior resistance to ADV and with an undetectable viral load at the start of the study, in 14 Spanish hospitals. The follow-up time for each patient was 48 wk after randomization, with quarterly visits in which the viral load, biochemical and serological parameters, adverse effects, adherence to treatment and consumption of hospital resources were analysed.RESULTS Forty-six patients were evaluated [median age: 55.4 years(30.2-75.2); 84.8% male], including 22 patients with TDF and 24 with LAM+ADV. During study development, hepatitis B virus DNA(HBV-DNA) remained undetectable, all patients remained HBe Ag negative, and hepatitis B surface antigen(HBs Ag) positive. Alanine aminotransferase(ALT) values at the end of the study were similar in the 2 groups(25.1± 7.65, TDF vs 24.22 ± 8.38, LAM+ADV, P = 0.646). No significant changes were observed in creatinine or serum phosphorus values in either group. No significant differences between the 2 groups were noted in the identification of adverse effects(AEs)(53.8%, TDF vs 37.5%, LAM+ADV, P = 0.170), and none of the AEs which occurred were serious. Treatment adherence was 95.5% and 83.3% in the TDF and the LAM+ADV groups, respectively(P = 0.488). The costs associated with hospital resource consumption were significantly lower with the TDF treatment than the LAM+ADV treatment(€4943 ± 1059 vs €5811 ± 1538, respectively, P < 0.001).CONCLUSION TDF monotherapy proved to be safe and not inferior to the LAM+ADV combination therapy in maintaining virologic response in patients with CHB and previous LAM failure. In addition, the use of TDF generated a significant savings in hospital costs. | Manuel Rodríguez Juan Manuel Pascasio Enrique Fraga Javier Fuentes Martín Prieto Gloria Sánchez-Antolín Jose Luis Calleja Esther Molina María Luisa García-Buey María Angeles Blanco Javier Salmerón María Lucía Bonet Jose Antonio Pons Jose Manuel González Miguel Angel Casado Francisco Jorquera 无 | 2017 | World Journal of Gastroenterology2017，23，41： | 16 |
| 2 | Application of near infrared reflectance spectroscopy to predict meat and meat products quality: A review显示文摘 | N. Prieto R. Roehe P. Lavín G. Batten S. Andrés | 2009 | Meat Science2009，，2： | 6 |
| 3 | Post-translational modifications of prostaglandin-endoperoxide synthase 2 in colorectal cancer:An update显示文摘The biosynthesis of prostanoids is involved in both physiological and pathological processes. The expression of prostaglandin-endoperoxide synthase 2(PTGS2; also known as COX-2) has been traditionally associated to the onset of several pathologies, from inflammation to cardiovascular, gastrointestinal and oncologic events. For this reason, the search of selective PTGS2 inhibitors has been a focus for therapeutic interventions. In addition to the classic non-steroidal anti-inflammatory drugs, selective and specific PTGS2 inhibitors, termed coxibs, have been generated and widely used. PTGS2 activity is less restrictive in terms of substrate specificity than the homeostatic counterpart PTGS1, and it accounts for the elevated prostanoid synthesis that accompanies several pathologies. The main regulation of PTGS2 occurs at the transcription level. In addition to this, the stability of the mRNA is finely regulated through the interaction with several cytoplasmic elements, ranging from specificmicroR NAs to proteins that control mR NA degradation. Moreover, the protein has been recognized to be the substrate for several post-translational modifications that affect both the enzyme activity and the targeting for degradation via proteasomal and non-proteasomal mechanisms. Among these modifications, phosphorylation, glycosylation and covalent modifications by reactive lipidic intermediates and by free radicals associated to the proinflammatory condition appear to be the main changes. Identification of these post-translational modifications is relevant to better understand the role of PTGS2 in several pathologies and to establish a correct analysis of the potential function of this protein in diseases progress. Finally, these modifications can be used as biomarkers to establish correlations with other parameters, including the immunomodulation dependent on molecular pathological epidemiology determinants, which may provide a better frame for potential therapeutic interventions. | Rafael I Jaén Patricia Prieto Marta Casado Paloma Martín-Sanz Lisardo Boscá | 2018 | World Journal of Gastroenterology2018，24，48： | 5 |
| 4 | Interplay between post-translational cyclooxygenase-2 modifications and the metabolic and proteomic profile in a colorectal cancer cohort显示文摘BACKGROUND Colorectal cancer(CRC) is the second most common cause of cancer death worldwide. It is broadly described that cyclooxygenase-2(COX-2) is mainly overexpressed in CRC but less is known regarding post-translational modifications of this enzyme that may regulate its activity, intracellular localization and stability. Since metabolic and proteomic profile analysis is essential for cancer prognosis and diagnosis, our hypothesis is that the analysis of correlations between these specific parameters and COX-2 state in tumors of a high number of CRC patients could be useful for the understanding of the basis of this cancer in humans.AIM To analyze COX-2 regulation in colorectal cancer and to perform a detailed analysis of their metabolic and proteomic profile.METHODS Biopsies from both healthy and pathological colorectal tissues were taken under informed consent from patients during standard colonoscopy procedure in the University Hospital of Bellvitge(Barcelona, Spain) and Germans Trias i Pujol University Hospital(Campus Can Ruti)(Barcelona, Spain). Western blot analysis was used to determine COX-2 levels. Deglycosylation assays were performed in both cells and tumor samples incubating each sample with peptide N-glycosidase F(PNGase F). Prostaglandin E2(PGE2) levels were determined using a specific ELISA. 1 H high resolution magic angle spinning(HRMAS) analysis was performed using a Bruker AVIII 500 MHz spectrometer and proteomic analysis was performed in a nano-liquid chromatography-tandem mass spectrometer(nano LC-MS/MS) using a QExactive HF orbitrap MS.RESULTS Our data show that COX-2 has a differential expression profile in tumor tissue of CRC patients vs the adjacent non-tumor area, which correspond to a glycosylated and less active state of the protein. This fact was associated to a lesser PGE2 production in tumors. These results were corroborated in vitro performing deglycosylation assays in HT29 cell line where COX-2 protein profile was modified after PNGase F incubation, showing higher PGE2 levels. Moreover,HRMAS analysis indicated that tumor tissue has altered metabolic features vs non-tumor counterparts, presenting increased levels of certain metabolites such as taurine and phosphocholine and lower levels of lactate. In proteomic experiments, we detected an enlarged number of proteins in tumors that are mainly implicated in basic biological functions like mitochondrial activity,DNA/RNA processing, vesicular trafficking, metabolism, cytoskeleton and splicing.CONCLUSION In our colorectal cancer cohort, tumor tissue presents a differential COX-2 expression pattern with lower enzymatic activity that can be related to an altered metabolic and proteomic profile. | Patricia Prieto Rafael I Jaén Daniel Calle María Gómez-Serrano Estefanía Nú?ez María Fernández-Velasco Paloma Martín-Sanz Sergio Alonso Jesús Vázquez Sebastián Cerdán Miguel ángel Peinado Lisardo Boscá | 2019 | World Journal of Gastroenterology2019，25，4： | 4 |
| 5 | Effect of Calcineurin Inhibitors in the Outcome of Liver Transplantation in Hepatitis C Virus-Positive Recipients显示文摘 | Marina Berenguer Victoria Aguilera Fernando San Juan Salvador Benlloch Angel Rubin Rafael López-Andujar Angel Moya Eugenia Pareja Eva Montalva Maria Yago Manuel de Juan José Mir Martín Prieto | 2010 | Transplantation2010，，11： | 3 |
| 6 | Capsule endoscopy capture rate: Has 4 frames-per-second any impact over 2frames-per-second?显示文摘AIM: To compare the current capsule and a new prototype at 2 and 4 frames-per-second, respectively, in terms of clinical and therapeutic impact. METHODS: One hundred patients with an indication for capsule endoscopy were included in the study. All procedures were performed with the new device(SB24). After an exhaustive evaluation of the SB24 videos, they were then converted to 'SB2-like' videos for their evaluation. Findings, frames per finding, and clinical and therapeutic impact derived from video visualization were analyzed. Kappa index for interobserver agreement and χ2 and Student's t tests for qualitative/quantitative variables, respectively, were used. Values of P under 0.05 were considered statistically significant.RESULTS: Eighty-nine out of 100 cases included in the study were ultimately included in the analysis. The SB24 videos detected the anatomical landmarks(Zline and duodenal papilla) and lesions in more patients than the 'SB2-like' videos. On the other hand, the SB24 videos detected more frames per landmark/lesion than the 'SB2-like' videos. However, these differences were not statistically significant(P > 0.05). Both clinical and therapeutic impacts were similar between SB24 and 'SB2-like' videos(K = 0.954). The time spent by readers was significantly higher for SB24 videos visualization(P < 0.05) than for 'SB2-like' videos when all images captured by the capsule were considered. However, these differences become non-significant if we only take into account small bowel images(P > 0.05).CONCLUSION: More frames-per-second detect more landmarks, lesions, and frames per landmark/lesion, but is time consuming and has a very low impact on clinical and therapeutic management. | Ignacio Fernandez-Urien Cristina Carretero Erika Borobio Ana Borda Emilio Estevez Sara Galter Begoa Gonzalez-Suarez Benito Gonzalez Marisol Lujan Jose Luis Martinez Vanessa Martínez Pedro Menchén Javier Navajas Vicente Pons Cesar Prieto Julio Valle | 2014 | World Journal of Gastroenterology2014，20，39： | 2 |
| 7 | Liver transplantation in patients with portal vein thrombosis显示文摘 | Gerardo Manzanet Fernando Sanjuán Paco Orbis Rafael López ángel Moya Manuel Juan Juanjo Vila Josep Asensi Pedro Sendra José Ru??z Mart??n Prieto José Mir | 2001 | Liver Transplantation2001，，2： | 1 |
| 8 | Potential use of near infrared reflectance spectroscopy (NIRS) for the estimation of chemical composition of oxen meat samples显示文摘 | Prieto N Andr6s S Gir/:ldez F J | 2006 | Meat Science2006，74，3： | 1 |
| 9 | Single drop microextraction and gas chromatography–mass spectrometry for the determination of diflufenican, mepanipyrim, fipronil, and pretilachlor in water samples显示文摘 | Lilia Araujo María E. Troconis Dalia Cubillán Jair Mercado Noreiva Villa Avismelsi Prieto | 2013 | Environmental Monitoring and Assessment2013，，12： | 1 |
| 10 | Potential use of near infrared reflectance spectroscopy (NIRS) for the eslimafion of chemical com- position of oxen meat samples显示文摘 | PRIETO N ANDRIS S GIRALDEZ F J | 2006 | Meat Science2006，74，3： | 1 |
| 11 | Early thnctional outcomes and histological analysis after spinal cord compression injury in rats 显示文摘 | Lonjon N Kouyoumdjian P Prieto M | 2010 | J Neurosurg Spine2010，1，： | 1 |
| 12 | Application of near infrared reflectance spectroscopy to predict meat and meat products quality: a review显示文摘 | Prieto N Roche R Lavln P | 2009 | Meat Science2009，83，2： | 1 |
| 13 | Effects of Intense Pulsed Light on Sundamaged Human Skin, Routine, and Ultra Structural Analysis 显示文摘 | PRIETO V G SADICK N S LLORETA J | 2002 | Lasers Surg Med2002，30，： | 1 |
| 14 | Tanycytes present in the adult rat mediobasal hypothalamus support the regeneration of monoaminergic axons 显示文摘 | Chauvet N Prieto M Alonso G | 1998 | Exp Neurol1998，151，1： | 1 |
| 15 | On-line application of visible and near infrared reflectance spectroscopy to predict chemical-physical and sensory characteristics of beef quality显示文摘 | Prieto N Ross D W Navajas E A | 2009 | Meat Science2009，83，1： | 1 |
| 16 | Ability of near infrared reflectance spectroscopy (NIRS) to estimate physical parameters of adult steers (oxen) and young cattle meat samples 显示文摘 | Prieto N Andres S Giraldez F J | 2008 | Meat Science2008，79，4： | 1 |
| 17 | Early functional outcomes and his- tological analysis after spinal cord compression injury in rats显示文摘 | Lonjon N Kouyoumdjian P Prieto M Bauchet L Haton H Gaviria M | 2010 | J Neurosurg Spine2010，12，： | 1 |
| 18 | Discrimination of adult steers (oxen) and young cattle ground meat samples by near infrared reflectance spectroscopy (NIRS) 显示文摘 | Prieto N Andres S Giraldez F J | 2008 | Meat Science2008，79，1： | 1 |
| 19 | Application of near infrared reflectance spectroscopy to predict meat and meat products quality: A review显示文摘 | Prieto N Roehe R Lavln P et ol | 2009 | Meat Sci2009，83，： | 1 |
| 20 | Early functional out-comes and histological analysis after spinal cord compression in-jury in rats显示文摘 | Lonjon N Kouyoumdjian P Prieto M | 2010 | J Neurosurg Spine2010，12，1： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员