| 1 | Single-cell RNA-seq data analysis on the receptor ACE2 expression reveals the potential risk of different human organs vulnerable to 2019-nCoV infection显示文摘It has been known that,the novel coronavirus,2019-nCoV,which is considered similar to SARS-CoV,invades human cells via the receptor angiotensin converting enzyme II(ACE2).Moreover,lung cells that have ACE2 expression may be the main target cells during 2019-nCoV infection.However,some patients also exhibit non-respiratory symptoms,such as kidney failure,implying that 2019-nCoV could also invade other organs.To construct a risk map of different human organs,we analyzed the single-cell RNA sequencing(scRNA-seq)datasets derived from major human physiological systems,including the respiratory,cardiovascular,digestive,and urinary systems.Through scRNA-seq data analyses,we identified the organs at risk,such as lung,heart,esophagus,kidney,bladder,and ileum,and located specific cell types(i.e.,type II alveolar cells(AT2),myocardial cells,proximal tubule cells of the kidney,ileum and esophagus epithelial cells,and bladder urothelial cells),which are vulnerable to 2019-nCoV infection.Based on the findings,we constructed a risk map indicating the vulnerability of different organs to 2019-nCoV infection.This study may provide potential clues for further investigation of the pathogenesis and route of 2019-nCoV infection. | Xin Zou Ke Chen Jiawei Zou Peiyi Han Jie Hao Zeguang Han | 2020 | Frontiers of Medicine2020，14，2： | 193 |
| 3 | An ethylene-induced NAC transcription factor acts as a multiple abiotic stress responsor in conifer显示文摘The proper response to various abiotic stresses is essential for plants’survival to overcome their sessile nature,especially for perennial trees with very long-life cycles.However,in conifers,the molecular mechanisms that coordinate multiple abiotic stress responses remain elusive.Here,the transcriptome response to various abiotic stresses like salt,cold,drought,heat shock and osmotic were systematically detected in Pinus tabuliformis(P.tabuliformis)seedlings.We found that four transcription factors were commonly induced by all tested stress treatments,while PtNAC3 and PtZFP30 were highly up-regulated and co-expressed.Unexpectedly,the exogenous hormone treatment assays and the content of the endogenous hormone indicates that the upregulation of PtNAC3 and PtZFP30 are mediated by ethylene.Time-course assay showed that the treatment by ethylene immediate precursor,1-aminocyclopropane-1-carboxylic acid(ACC),activated the expression of PtNAC3 and PtZFP30 within 8 hours.We further confirm that the PtNAC3 can directly bind to the PtZFP30 promoter region and form a cascade.Overexpression of PtNAC3 enhanced unified abiotic stress tolerance without growth penalty in transgenic Arabidopsis and promoted reproductive success under abiotic stress by shortening the lifespan,suggesting it has great potential as a biological tool applied to plant breeding for abiotic stress tolerance.This study provides novel insights into the hub nodes of the abiotic stresses response network as well as the environmental adaptation mechanism in conifers,and provides a potential biofortification tool to enhance plant unified abiotic stress tolerance. | Fangxu Han Peiyi Wang Xi Chen Huanhuan Zhao Qianya Zhu Yitong Song Yumeng Nie Yue Li Meina Guo Shihui Niu | 2023 | Horticulture Research2023，10，8： | 0 |
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