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| 1 | Scutellarin protects oxygen/glucose-deprived astrocytes and reduces focal cerebral ischemic injury显示文摘Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2(NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity. Cultures of primary astrocytes isolated from the cerebral cortex of neonatal Sprague-Dawley rats were pretreated with 2, 10 or 50 μM scutellarin for 30 minutes. The astrocytes were then subjected to oxygen/glucose deprivation by incubation for 2 hours in glucose-free Dulbecco's modified Eagle's medium in a 95% N2/5% CO_2 incubator, followed by simulated reperfusion for 22 hours. Cell viability was assessed by cell counting kit-8 assay. Expression levels of NOX2, connexin 43 and caspase-3 were assessed by western blot assay. Reactive oxygen species were measured spectrophotometrically. Pretreatment with 10 or 50 μM scutellarin substantially increased viability, reduced the expression of NOX2 and caspase-3, increased the expression of connexin 43, and diminished the levels of reactive oxygen species in astrocytes subjected to ischemia-reperfusion. We also assessed the effects of scutellarin in vivo in the rat transient middle cerebral artery occlusion model of cerebral ischemia-reperfusion injury. Rats were given intraperitoneal injection of 100 mg/kg scutellarin 2 hours before surgery. The Bederson scale was used to assess neurological deficit, and 2,3,5-triphenyltetrazolium chloride staining was used to measure infarct size. Western blot assay was used to assess expression of NOX2 and connexin 43 in brain tissue. Enzyme-linked immunosorbent assay was used to detect 8-hydroxydeoxyguanosine(8-OHd G), 4-hydroxy-2-nonenal(4-HNE) and 3-nitrotyrosin(3-NT) in brain tissue. Immunofluorescence double staining was used to determine the co-expression of caspase-3 and Neu N. Pretreatment with scutellarin improved the neurological function of rats with focal cerebral ischemia, reduced infarct size, diminished the expression of NOX2, reduced levels of 8-OHd G, 4-HNE and 3-NT, and reduced the number of cells co-expressing caspase-3 and Neu N in the injured brain tissue. Furthermore, we examined the effect of the NOX2 inhibitor apocynin. Apocynin substantially increased connexin 43 expression in vivo and in vitro. Collectively, our findings suggest that scutellarin protects against ischemic injury in vitro and in vivo by downregulating NOX2, upregulating connexin 43, decreasing oxidative damage, and reducing apoptotic cell death. | Jing-Bo Sun Yan Li Ye-Feng Cai Yan Huang Shu Liu Patrick KK Yeung Min-Zhen Deng Guang-Shun Sun Prince LM Zilundu Qian-Sheng Hu Rui-Xin An Li-Hua Zhou Li-Xin Wang Xiao Cheng | 2018 | Neural Regeneration Research2018，13，8： | 16 |
| 2 | Epac2-deficiency leads to more severe retinal swelling, glial reactivity and oxidative stress in transient middle cerebral artery occlusion induced ischemic retinopathy显示文摘Ischemia occurs in diabetic retinopathy with neuronal loss, edema, glial cell reactivity and oxidative stress. Epacs, consisting of Epac1 and Epac2, are c AMP mediators playing important roles in maintenance of endothelial barrier and neuronal functions. To investigate the roles of Epacs in the pathogenesis of ischemic retinopathy, transient middle cerebral artery occlusion(t MCAO) was performed on Epac1-deficient(Epac1-/-) mice, Epac2-deficient(Epac2-/-) mice, and their wild type counterparts(Epac1+/+ and Epac2+/+). Two-hour occlusion and 22-hour reperfusion were conducted to induce ischemia/reperfusion injury to the retina. After t MCAO, the contralateral retinae displayed similar morphology between different genotypes. Neuronal loss, retinal edema and increase in immunoreactivity for aquaporin 4(AQP4), glial fibrillary acidic protein(GFAP), peroxiredoxin 6(Prx6) were observed in ipsilateral retinae. Epac2-/- ipsilateral retinae showed more neuronal loss in retinal ganglion cell layer, increased retinal thickness and stronger immunostaining of AQP4, GFAP, and Prx6 than those of Epac2+/+. However, Epac1-/- ipsilateral retinae displayed similar pathology as those in Epac1+/+ mice. Our observations suggest that Epac2-deficiency led to more severe ischemic retinopathy after retinal ischemia/reperfusion injury. | LIU Jin YEUNG Patrick Ka Kit CHENG Lu LO Amy Cheuk Yin CHUNG Stephen Sum Man CHUNG Sookja Kim | 2015 | Science China(Life Sciences)2015，58，6： | 12 |
| 3 | Die design and process optimization of die cast V6 engine blocks显示文摘The use of aluminum, particularly for engine blocks, has grown considerably in the past ten years, and continues to rise in the automotive industry. In order to enhance the quality and engineering functionality of die cast engine blocks, die design and processes have to be optimized. In this study, a computer simulation software, MAGMAsoft, as an advanced tool for optimizing die design and casting process, was employed to virtually visualize cavity filling and patterns of a V6 engine block. The original die design and process was simulated first to establish a baseline. A reality check was used to verify the predicted results. Then, the die modification with a different runner system was made by using a CAD software, Unigraphics (UG). The simulation on combinations of the modified die design and revised process was performed to examine the effect of die modification and process change on flow filling of V6 engine blocks. The simulated prediction indicates that the enhancement of cavity filling due to the die and process modification minimizes the occurrence of defects during casting, and consequently improves the quality of blocks. The results of mechanical testing show a significant increase in fatigue strengths, and a moderately improvement on tensile properties for the blocks die cast with the new die design and process in comparison with those produced by the original ones. | Henry HU Yeouli CHU Patrick CHENG | 2005 | China Foundry2005，2，1： | 3 |
| 4 | High-speed dual-view band-limited illumination profilometry using temporally interlaced acquisition显示文摘We report dual-view band-limited illumination profilometry(BLIP)with temporally interlaced acquisition(TIA)for high-speed,three-dimensional(3D)imaging.Band-limited illumination based on a digital micromirror device enables sinusoidal fringe projection at up to 4.8 kHz.The fringe patterns are captured alternately by two high-speed cameras.A new algorithm,which robustly matches pixels in acquired images,recovers the object’s 3D shape.The resultant TIA–BLIP system enables 3D imaging over 1000 frames per second on a field of view(FOV)of up to 180 mm×130 mm(corresponding to 1180×860 pixels)in captured images.We demonstrated TIA–BLIP’s performance by imaging various static and fast-moving 3D objects.TIA–BLIP was applied to imaging glass vibration induced by sound and glass breakage by a hammer.Compared to existing methods in multiview phase-shifting fringe projection profilometry,TIA–BLIP eliminates information redundancy in data acquisition,which improves the 3D imaging speed and the FOV.We envision TIA–BLIP to be broadly implemented in diverse scientific studies and industrial applications. | Cheng Jiang Patrick Kilcullen Yingming Lai Tsuneyuki Ozaki Jinyang Liang | 2020 | Photonics Research2020，8，11： | 3 |
| 5 | Effects of Soil Properties on the Transfer of Cadmium from Soil to Wheat in the Yangtze River Delta Region, China—a Typical Industry–Agriculture Transition Area显示文摘 | Cheng Wang Junfeng Ji Zhongfang Yang Lingxiao Chen Patrick Browne Ruilian Yu | 2012 | Biological Trace Element Research2012，，2： | 1 |
| 6 | A Framework for Benchmarking the Value Management Process显示文摘 | Patrick Sik-wah Fong Qiping Shen Eddie WL Cheng | 2001 | Benchmarking2001，8，4： | 1 |
| 7 | Principles of ECAP–Conform as a continuous process for achieving grain refinement: Application to an aluminum alloy显示文摘 | Cheng Xu Steven Schroeder Patrick B. Berbon Terence G. Langdon | 2009 | Acta Materialia2009，，4： | 1 |
| 8 | Axillary lymph node status of operable breast cancers by combined steroid receptor and HER-2 status: triple positive tumours are more likely lymph node positive显示文摘 | Ben Calster Isabelle Vanden Bempt Maria Drijkoningen Nathalie Pochet Jiqiu Cheng Sabine Huffel Wouter Hendrickx Julie Decock Huei-Jean Huang Karin Leunen Frederic Amant Patrick Berteloot Robert Paridaens Hans Wildiers Erik Limbergen Caroline Weltens Dirk | 2009 | Breast Cancer Research and Treatment2009，，1： | 1 |
| 9 | Raman microscopy mapping for the purity assessment of chirality enriched carbon nanotube networks in thin- film transistors显示文摘With recent improvements in carbon nanotube separation methods, the accurate determination of residual metallic carbon nanotubes in a purified nanotube sample is important, particularly for those interested in using semiconducting single-walled carbon nanotubes (SWCNTs) in electronic device applications such as thin-film transistors (TFTs). This work demonstrates that Raman microscopy mapping is a powerful characterization tool for quantifying residual metallic carbon nanotubes present in highly enriched semiconducting nanotube networks. Raman mapping correlates well with absorption spectroscopy, yet it provides greater differentiation in purity. Electrical data from TFTs with channel lengths of 2.5 and 5μ m demonstrate the utility of the method. By comparing samples with nominal purities of 99.0% and 99.8%, a clear differentiation can be made when evaluating the current on/off ratio as a function of channel length, and thus the Raman mapping method provides a means to guide device fabrication by correlating SWCNT network density and purity with TFT channel scaling. | Zhao Li Jianfu Ding Paul Finnie Jacques Lefebvre Fuyong Cheng ChristopherT. Kingston Patrick R. L. Malenfant | 2015 | Nano Research2015，8，7： | 1 |
| 10 | Second malignant neoplasms in childhood cancer survivors in a tertiary paediatric oncology centre in Hong Kong, China显示文摘背景童年癌症幸存者在这研究的第二个恶意的 neoplasms.The 目的开发的风险是在香港在一个第三级的儿科学的肿瘤学中心在童年癌症幸存者评估发生,风险因素和第二个恶意的瘤的结果,我们执行了的 China.Methods 有在威尔士医院,香港,在1984年5月之间的中国和2009年6月 .Case 的王子在孩子的癌症中心治疗的童年癌症的病人的回顾的评论病人记录 | SUN Wai-fun CHENG Frankie Wai-tsoi LEE Vincent LEUNGWing-kwan SHING Ming-kong YUEN Patrick Man-pan LI Chi-kong | 2011 | Chinese Medical Journal2011，，22： | 1 |
| 11 | Synthesis and characterization of water soluble chiral conducting polymer nanocomposites显示文摘 | McCarthy Patrick A Huang Jianyu Yang Sze cheng | 2002 | Langmuir2002，18，1： | 1 |
| 12 | Impact of multiple balloon inflations during primary percutaneous coronary intervention on infarct size and long-term clinical outcomes in ST-segment elevation myocardial infarction: real-world postconditioning显示文摘 | Tuncay Yetgin Michael Magro Olivier C. Manintveld Sjoerd T. Nauta Jin M. Cheng Corstiaan A. Uil Cihan Simsek Ferry Hersbach Ron T. Domburg Eric Boersma Patrick W. Serruys Dirk J. Duncker Robert-Jan M. Geuns Felix Zijlstra | 2014 | Basic Research in Cardiology2014，，2： | 1 |
| 13 | Consumers’ attributions and brand evaluations in product‐harm crises: The role of implicit theories of personality显示文摘 | Cheng‐Yue Yin Hong‐Yan Yu Patrick Poon | 2016 | J. Consumer Behav2016，，1： | 1 |
| 14 | Pre-acclimation of a wastewater inoculum to cellulose in an aqueous–cathode MEC improves power generation in air–cathode MFCs显示文摘 | Shaoan Cheng Patrick Kiely Bruce E. Logan | 2010 | Bioresource Technology2010，，1： | 1 |
| 15 | Gerard downey predictionof beef eating quality from colour, marbling and wavelet texturefeatures 显示文摘 | Patrick J Da W S Cheng J D | 2008 | Meat Sci2008，80，： | 1 |
| 16 | Sequencing a mouse acute promyelocytic leukemia genome reveals genetic events relevant for disease progression显示文摘 | Wartman Lukas D Larson David E Xiang Zhifu Ding Li Chen Ken Lin Ling Cahan Patrick Klco Jeffery M Welch John S Li Cheng Payton Jacqueline E Uy Geoffrey L Varghese Nobish Ries Rhonda E Hoock Mieke Koboldt Daniel C McLellan Michael D Schmid | 2011 | Journal of Clinical Investigation2011，，： | 1 |
| 17 | Lighthbsotpti~ on and Partitioning In Relation to Nitrogen Content in 'Fuji' Apple Leaves显示文摘 | Lailiang Cheng LeslieH Fuchigami Patrick | 2000 | J Amer Soc Hort Sci2000，125，5： | 1 |
| 18 | ZnO Nanowire Arrays for Enhanced Photocurrent in PbS Quantum Dot Solar Cells显示文摘 | Joel Jean Sehoon Chang Patrick R. Brown Jayce J. Cheng Paul H. Rekemeyer Moungi G. Bawendi Silvija Grade?ak Vladimir Bulovi? | 2013 | Adv. Mater2013，，20： | 1 |
| 19 | Heme Oxygenase 1 Determines Atherosclerotic Lesion Progression Into a Vulnerable Plaque显示文摘 | Caroline Cheng Annemarie M. Noordeloos Viktoria Jeney Miguel P. Soares Frans Moll Gerard Pasterkamp Patrick W. Serruys Henricus J. Duckers | 2009 | Circulation2009，，23： | 1 |
| 20 | The effect of dietary n-3 HUFA levels and DHA/EPA ratios on growth, survival and osmotic stress tolerance of Chinese mitten crab Eriocheir sinensis larvae显示文摘 | Liying Sui Mathieu Wille Yongxu Cheng Patrick Sorgeloos | 2007 | Aquaculture2007，，1： | 1 |
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