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| 1 | Isolation and characterisation of human gingival margin-derived STRO-1/MACS^+ and MACS^-cell populations显示文摘Recently,gingival margin-derived stem/progenitor cells isolated via STRO-1/magnetic activated cell sorting(MACS) showed remarkable periodontal regenerative potential in vivo.As a second-stage investigation,the present study's aim was to perform in vitro characterisation and comparison of the stem/progenitor cell characteristics of sorted STRO-1-positive(MACS^+) and STRO-1-negative(MACS^-) cell populations from the human free gingival margin.Cells were isolated from the free gingiva using a minimally invasive technique and were magnetically sorted using anti-STRO-1 antibodies.Subsequently,the MACS^+ and MACS^- cell fractions were characterized by flow cytometry for expression of CD14,CD34,CD45,CD73,CD90,CD105,CD146/MUC18 and STRO-1.Colony-forming unit(CFU) and multilineage differentiation potential were assayed for both cell fractions.Mineralisation marker expression was examined using real-time polymerase chain reaction(PCR).MACS^+ and MACS- cell fractions showed plastic adherence.MACS^+ cells,in contrast to MACS- cells,showed all of the predefined mesenchymal stem/progenitor cell characteristics and a significantly higher number of CFUs(P<0.01).More than 95%of MACS^+ cells expressed CD105,CD90 and CD73;lacked the haematopoietic markers CD45,CD34 and CD14,and expressed STRO-1 and CD146/MUC18.MACS- cells showed a different surface marker expression profile,with almost no expression of CD14 or STRO-1,and more than 95%of these cells expressed CD73,CD90 and CD146/MUC18,as well as the haematopoietic markers CD34 and CD45 and CD105.MACS^+ cells could be differentiated along osteoblastic,adipocytic and chondroblastic lineages.In contrast,MACS- cells demonstrated slight osteogenic potential.Unstimulated MACS^+ cells showed significantly higher expression of collagen I(P<0.05) and collagen III(P<0.01),whereas MACS^- cells demonstrated higher expression of osteonectin(P<0.05;MannWhitney).The present study is the first to compare gingival MACS^+ and MACS- cell populations demonstrating that MACS^+ cells,in contrast to MACS- cells,harbour stem/progenitor cell characteristics.This study also validates the effectiveness of the STRO-l/MACS^+technique for the isolation of gingival stem/progenitor cells.Human free gingival margin-derived STRO-1/MACS^+ cells are a unique renewable source of multipotent stem/progenitor cells. | Karim M Fawzy El-Sayed Sebastian Paris Christian Graetz Neemat Kassem Mohamed Mekhemar Hendrick Ungefroren Fred Fndrich Christof Drfer | 2015 | International Journal of Oral Science2015，7，2： | 6 |
| 2 | 过瘤胃蛋氨酸、赖氨酸和组氨酸对泌乳奶牛生产性能的影响显示文摘试验旨在研究饲料中单独添加或综合添加过瘤胃(RP)蛋氨酸、赖氨酸和组氨酸及可代谢蛋白(MP)对泌乳后期奶牛生产性能的影响。试验选取72头荷斯坦奶牛,随机分为6组,试验期9周。奶牛随机分配到6个试验组中,2周作为1个变量周期:MPA组,根据美国国家科学研究委员会(2001)标准,在饲料中添加调节MP到243 g·d-1;MPD组,不添加MP,MP含量低于标准量54 g·d-1;MPDM组,在MPD组的基础上加入RP蛋氨酸;MPDL组,在MPD组的基础上加入RP赖氨酸;MPDH组,在MPD组的基础上加入组氨酸;MPDMLH组,在MPD组的基础上加入RP蛋氨酸、RP赖氨酸和RP组氨酸。试验结果表明,与MPA组对比,MPD组的干物质采食量(DMI)、产奶量、乳成分(乳脂肪、乳蛋白和乳糖)及能量校正的牛乳(ECM)、ECM饲料利用效率、牛奶和血浆中的N均降低。在MPD基础上补充RP赖氨酸可使乳蛋白率、血浆中葡萄糖浓度增加,且牛奶中N元素含量有增加的趋势;补充RP组氨酸可使DMI、牛奶浓缩蛋白增加,且乳脂肪、乳蛋白和ECM显著增加;补充3种RP氨基酸时,增加了乳蛋白率,使DMI和乳脂率有增加趋势,也提高了乳脂肪、乳蛋白含量和ECM及ECM饲料利用效率。与MPA相比,MPD组牛奶中N的含量有降低趋势,且血浆中必需氨基酸(除赖氨酸和蛋氨酸)浓度均降低。补充RP赖氨酸、RP蛋氨酸和RP组氨酸提高了血浆中蛋氨酸(除MPDM组)、赖氨酸和组氨酸浓度。饲喂MPD的奶牛血红蛋白浓度降低,血浆生长素含量高于MPA组。MPD组牛奶中总饱和脂肪酸浓度有高于MPA和MPDMLH组的趋势。MPD组的多元不饱和脂肪、牛奶中奇链与支链脂肪酸含量与MPA组相比,有降低趋势。试验结果表明,对于饲料中缺乏MP的奶牛,在饲料中添加组氨酸和添加3种RP氨基酸(赖氨酸、蛋氨酸和组氨酸)均有提高牛奶产量和牛奶组分含量的可能性。 | Giallongo F Harper M T Oh J Lopes J C Lapierre H Patton R A Parys C Shinzato I Hristov A N | 2016 | 饲料博览2016，0，7： | 4 |
| 3 | Risk-adjustment in hepatobiliarypancreatic surgery显示文摘AIM: The present study evaluates the performance of the POSSUM, the American Society of Anesthetists (ASA),APACHE and Childs classification in predicting mortality and morbidity in hepatopancreaticobiliary (HPB) surgery.We describe especially the limitations and advantages of risk in stratifying the patients.METHODS: We investigated 177 randomly chosen patients undergoing eledive complex HPB surgery in a single institution with a total of 71 pre-operative and intra-operative risk factors.Primary endpoint was in-hospital mortality and morbidity.Ordered logistic regression analysis was used to identify individual predictors of operative morbidity and mortality.RESULTS: The operative mortality in the series was 3.95% .This compared well with the p-POSSUM and APACHE predicted mortality of 4.31% and 4.29% respectively. Postoperative complications amounted to 45% with 24 (13.6%)patients having a major adverse event. On multivariate analysis the pre-operative POSSUM physiological score (OR = 1.18,P = 0.009) was superior in predicting complications compared to the ASA (P = 0.108), APACHE (P = 0.117)or Childs classification (P = 0.136). In addition, serum sodium, creatinine, international normalized ratio (INR),pulse rate, and intra-operative blood loss were independent risk factors. A combination of the POSSUM variables and INR offered the optimal combination of risk factors for risk prognostication in HPB surgery.CONCLUSION: Morbidity for elective HPB surgery can be accurately predicted and applied in everyday surgical practice as an adjunct in the process of informed consent and for effective allocation of resources for intensive and high-dependency care facilities. | Hemant M Kocher Paris P Tekkis Palepu Gopal Ameet G Patel Simon Cottam Irving S Benjamin | 2005 | World Journal of Gastroenterology2005，11，16： | 3 |
| 4 | Tunor response to radiotherapy regulated by endothelial cell apoptosis 显示文摘 | Carcia-Barros M Paris F Cordon-Cardo C etat | 2003 | Science2003，300，5622： | 1 |
| 5 | Gene expression of humanbeta-defensins in healthy and inflamed human dental pulps显示文摘 | Paris S Wolgin M Kielbassa AM | 2009 | Bi-ology2009，35，4： | 1 |
| 6 | A physicoempirical model to predict the soil moisture characteristic from particle-size distribution and bulk density data 显示文摘 | ARYA L M PARIS J F | 1981 | Soil Science Society of America Journal1981，45，11： | 1 |
| 7 | Elastic field equations for blunt cracks with reference to stress corrosion cracking 显示文摘 | Creager M Paris P C | 1967 | International Journal of Fracture Mechanics1967，3，： | 1 |
| 8 | Two distinct tumor suppressor loci within chromosome 11P15 implicated In breast cancer progression and maetstasis显示文摘 | Kamik P Paris M Williajns B | 1998 | Hum Mol Genet1998，7，： | 1 |
| 9 | A rational analytic theory of fatigue显示文摘 | PARIS P C GOMEZ M P ANDERSON W P | 1961 | The Trend in Engineering1961，，13： | 1 |
| 10 | Elastic field equations for blunt cracks with reference to stress corrosion cracking显示文摘 | Creager M Paris P C | 1967 | International Jour- nal of Fatigue1967，3，： | 1 |
| 11 | A physicoempirical model to predict the soil moisture characteristic from partical - size distribution and bulk density data 显示文摘 | Arya L M Paris J F | 1981 | Soil Science Society of America Journal1981，45，： | 1 |
| 12 | Two-scale Tone Manage- ment for Photographic Look显示文摘 | BAE S M PARIS S DURAND F E | 2006 | ACM Transactions on Graph- ics2006，25，3： | 1 |
| 13 | A physicoempirical model to predict the soil moisture characteristic from particle-size distribution and bulk density data 显示文摘 | Arya L M Paris J F | 1981 | Soil Science Society of America Journal1981，45，： | 1 |
| 14 | A physicoempirical model to predict the soil moisture characteristic from particle size distribu tion and bulk density data显示文摘 | Arya L M Paris J F | 1981 | Soil Science Society of A merica Journal1981，45，6： | 1 |
| 15 | A physicoempirical model to predict the soil moisture characteristic from particle - size distribution and bulk density data 显示文摘 | Arya L M Paris J F | 1981 | Soil Science Society of America Journal1981，45，： | 1 |
| 16 | A rational analytic theory of fatigue 显示文摘 | Paris P C Gomez M P Anderson W E | 1961 | The Trend Engineering1961，13，1： | 1 |
| 17 | Loop-mediated isother- mal PCR (LAMP) for the diagnosis of falciparum malaria显示文摘 | Paris DH Imwong M Faiz AM | 2007 | Am J Trop Med Hyg2007，77，： | 1 |
| 18 | Analysis of load-displacement relationships to determine J-R curve and tearing instability material properties显示文摘 | ERNST H A PARIS P C ROSSOW M | 1979 | Fracture Mechanics ASTM STP 677 American Society for Testing and Materials1979，1979，： | 1 |
| 19 | A physicoempirical model to predict the soil moisture characteristic from particle - size distribution and bulk density data 显示文摘 | Arya L M Paris J F | 1981 | Soil Science Society of America Journal1981，45，： | 1 |
| 20 | Loop-mediated isothermal PCR(LAMP) for the diagnosis of falciparum malaria显示文摘 | Paris DH Imwong M Faiz AM | 2007 | Am J Trop Med Hyg2007，77，5： | 1 |
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