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| 1 | Resveratrol protects PC12 cells against OGD/ R-induced apoptosis via the mitochondrial-mediated signaling pathway显示文摘在这研究,我们对导致的氧葡萄糖 deprivation/reoxygenation (OGD/R ) 调查了 resveratrol 的 neuroprotective 潜力在区分得好的 PC12 房间和内在的机制的 apoptotic 损坏。房间当面在正常状况或 OGD/R 下面被孵化或 10 M resveratrol 的缺席。房间生存能力与 methyl-thiazolyl-tetrazolium (MTT ) 被决定试金。Apoptotic 比率与 Hoechst 33342 染色和 Annexin V-FITC/PI 两倍染色被决定。氧化应力被测量细胞内部的反应的氧评估 superoxide dismutase (草皮) 和过氧化氢酶(猫) 的种类(ROS ) , mitochondrial superoxide, malondialdehyde (MDA ) 内容,和这些活动。细胞内部的钙([Ca 2+]i) 被 Fluo-3/AM 估计。mitochondrial 膜潜力(MMP ) 被 5,5,6,6-tetrachloro-1,1,3,3-tetraethyl-benzimidazolyl-carbocyanine 碘化物(JC-1 ) 和玫瑰精评估 123 (Rh123 ) 。mitochondrial 渗透转变毛孔(MPTP ) 的开始被 Calcein/Co 2+-quenching 技术决定。细胞色素 c, Bcl-2, Bax,劈开的 caspase-9,和劈开的 caspase-3 的蛋白质层次被西方的污点分析检测。结果证明 10 M resveratrol 稀释了 OGD/R-induced 房间生存能力损失和房间 apoptosis,它在在 SOD 和猫活动的 MDA 内容和增加与减少被联系。而且,细胞内部的 ROS 和 mitochondrial superoxide 的累积,骚乱[Ca 2+]i 动态平衡, MMP 的减小, MPTP 的开始,和在 OGD/R-injured 房间观察的 mitochondrial 细胞色素 c 的版本,在调停 mitochondrial 的 apoptotic 小径上显示了一个开关,都被 resveratrol 颠倒。这些结果建议 resveratrol 管理可以经由 modulating 起一个 neuroprotective 作用在 OGD/R-induced PC12 房间损害的调停 mitochondrial 的发信号小径。 | Xuan Liu Xiangyang Zhu Miao Chen Qinmin Ge Yong Shen Shuming Pan | 2016 | Acta Biochimica et Biophysica Sinica2016，48，4： | 8 |
| 2 | The role of charged multivesicular body protein 5 in programmed cell death in leukemic cells显示文摘人(CHMP5 ) 现代人的控告的 multivesicular 身体蛋白质 5 是 multivesicular 身体的一个成员,它用作 anti-apoptotic 蛋白质并且被认为参予引起白血病。在这研究,一条短发卡的基于 RNA 的 RNA 干扰途径被用来在白血病的房间线 U937 禁止 CHMP5 的表示。在 CHMP5 被禁止以后,抗体 microarray 和西方的污点分析被用来在规划房间死亡(PCD ) 学习变化小径。PCD 能被分类进三种类型:apoptosis,坏死,和 autophagy。结果显示出那 caspase 3 在 CHMP5 缺乏的 U937 房间被激活,显示 apoptotic 小径被激活,尽管既不内在外来的 apoptotic 小径也没被激活。我们的结果也证明 Granzyme B/Perforin apoptotic 小径被 CHMP5 silencing 激活。坏死被 caspase 独立的刽子手激活。在这研究,我们证明导致 apoptosis 调停蛋白质的坏死的 PCD 小径在 CHMP5 抑制以后被激活。autophagic PCD 没发生在 CHMP5 缺乏的 U937 房间,这被发现。在结论,在 CHMP5 抑制,两条 Granzyme B/Perforin apoptotic 小径和导致 apoptosis 以后,调停因素的坏死的小径被激活,当 autophagic 小径没被激活时。 | Hairong Wang Jia Liu Feilong Wang Miao Chen Zhenyu Xiao Renrong OuYang Aihua Fei Yanlin Shen Shuming Pan | 2013 | Acta Biochimica et Biophysica Sinica2013，45，5： | 3 |
| 3 | 老年人与交通事故的调查分析(英文)显示文摘目的中国目前正在经历老年人口与交通车辆的同时快速增长。随着人口的老龄化加剧以及社会的发展,老年驾驶者人数将预期明显增加。国际上对老年人交通安全的经验可能对中国即将增加的老年驾驶者有借鉴作用。方法检索并分析了中国以及一些发达国家,如美国等有关老年驾驶者以及交通事故安全的论文及论著。结果尽管老年人较少开车且普遍地使用安全带,但他们交通事故发生数以及死亡率较其他年龄组高。老年人视觉以及感知功能的损害也增加了发生交通事故的危险性,同时也产生了其独特的事故形式。与年轻人相比,在相同的受伤程度下,老年人体格上的衰退和疾病因素可能对会产生更坏的结果。虽然老年驾驶者在中国还是少数,与其他年龄组相比,老年人有较高的交通事故伤害率、步行者死亡率和较高的农村老年人受害率。结论国际上对老年人交通安全的经验,比如更好的道路安全设计,高可视安全标识和安全带法律的实施,安全带使用的公共教育,以及老年驾驶证的更新等对中国的交通安全设计者以及法律的制定者可能会有帮助。这些经验对损伤预防研究者在老年人交通事故危险因素的识别方面,以及对工程师在驾驶环境和车辆设计等方面也有着重要的意义。 | HENG Kenneth PAN Shuming LAYDE M Peter ZHU Shankuan | 2006 | 中华急诊医学杂志2006，15，1： | 3 |
| 4 | Red cell distribution width as a novel predictor of mortality in ICU patients显示文摘 | Feilong Wang Wenzhi Pan Shuming Pan Junbo Ge Shuyun Wang Miao Chen | 2011 | Annals of Medicine2011，，1： | 1 |
| 5 | Red cell distribution width as a novel predictor of mortality in ICU patients 显示文摘 | Feilong Wang Wenzhi Pan Shuming Pan | 2011 | Annals of Medicine2011，4，3： | 1 |
| 6 | A Highly Sensitive In-Situ Turbidity Sensor With Low Power Consumption显示文摘一高度敏感在原处,有低电源消费的混浊传感器在这被求婚并且评估学习。满足实际要求在原处察觉,我们设计了轻散布路径,不透水的机械结构,和极端弱的散布光检测方法。实验证明传感器与 025 FTU 的集中范围和 0.9999 的 R 平方有 0.0076 FTU 的敏感。传感器能深入地 1000 m 的承受水压力并且在活跃模式有低电源消费 10.4 妈,有 8.4 V 的供应电压的睡觉模式 65 A。南部的中国海浮标实验显示传感器能工作很好在实际在原处环境。与另外的公司的传感器比较,我们的传感器相对有更全面的性能。 | Yi Hu Lei Sun Shuming Ye Hang Chen Kai Jiang Jianming Pan | 2014 | Photonic Sensors2014，4，1： | 1 |
| 7 | 显示文摘 | Pan Shuming Zhao Zhibo Jin Hanmin | 1989 | Journal of Magnetism and Magnetic Materials1989，78，3： | 1 |
| 8 | Long-term correction of hemophilia B through CRISPR/Cas9 induced homology-independent targeted integration显示文摘CRISPR/Cas9-mediated site-specific insertion of exogenous genes holds potential for clinical applications.However,it is still infeasible because homologous recombination(HR)is inefficient,especially for nondividing cells.To overcome the challenge,we report that a homology-independent targeted integration(HITI)strategy is used for permanent integration of high-specificity-activity Factor IX variant(F9 Padua,R338L)at the albumin(Alb)locus in a novel hemophilia B(HB)rat model.The knock-in efficiency reaches 3.66%,as determined by droplet digital PCR(dd PCR).The clotting time is reduced to a normal level four weeks after treatment,and the circulating factor IX(FIX)level is gradually increased up to 52%of the normal level over nine months even after partial hepatectomy,demonstrating the amelioration of hemophilia.Through primer-extension-mediated sequencing(PEM-seq),no significant off-target effect is detected.This study not only provides a novel model for HB but also identifies a promising therapeutic approach for rare inherited diseases. | Xi Chen Xuran Niu Yang Liu Rui Zheng Lei Yang Jian Lu Shuming Yin Yu Wei Jiahao Pan Ahmed Sayed Xueyun Ma Meizhen Liu Fengxiang Jing Mingyao Liu Jiazhi Hu Liren Wang Dali Li | 2022 | Journal of Genetics and Genomics2022，49，12： | 0 |
| 9 | (Nd_(0.9)Dy_(0.1))_(16)Co_(5)Fe_(70)Nb_(2)B_(7) PERMANENT MAGNETIC ALLOY AND BEHAVIOUR OF Nb显示文摘The magnetic properties of (Nd_(0.9)Dy_(0.1))_(16)Co_(5)Fe_(70)Nb_(2)B_(7) permanent magnetic alloy prepared by powder metallurgy are:Br=1.08T,_(1)H_(C)=1620kA/m,(BH)_(max)=208kJ/m^(3).The behaviour of Nb in this alloy was studied by joint methods of dynamic observation by high voltage electron microscope(HVEM)and Mossbauer effects.With Nb and Co substituting parts of Fe and adding a little Dy,practical magnetic alloy with excellent properties can be obtained.The thermal stability of it is 80 ℃ higher than that of ternary Nd-Fe-13 magnet.The study shows that adding Nb into Nd-Fe-B can make the intrinsic coercivity appear peak value at about Nb=2%(in mole fraction).Nb mainly enters into Nd-rich and B-rich phases,a little into Nd_(2)Fe_(14)B phase. | Pan Shuming Pan Feng Ma Ruzhang | 1996 | Transactions of Nonferrous Metals Society of China1996，6，1： | 0 |
| 10 | THE RELATION BETWEEN THE TEMPERING EFFECTS AND THE INTRINSIC COERCIVITY OF SINTERED SmCo5 ALLOY AT 750℃显示文摘The relation between the tempering effects of sinterd SmCo^(5)alloy at 750℃and its intrinsic coercivity(i^(Hc))has been studied by the use of photoelectron energy spectrum.X-ray diffraction and high-voltage electron microscope.The result is that the cause of iHc dropping seriously for sintered SmCo_(5)alloy tempered at 750 t is not the eutectoid decomposition of SmCo_(5)and the increase of oxygen.In fact,iHcdropping is caused by that some defect-rich regions in Sm_(2)Co_(17)decomposed form SmC0_(5)from nucleation centers in reversed magnetization course. | Pan Shuming | 1992 | Transactions of Nonferrous Metals Society of China1992，2，1： | 0 |
| 11 | Nocturnal urine 6-hydroxy sulfate melatonin is associated with the outcome of subjects with sepsis显示文摘Background:It is imperative to identify potential biomarkers for early diagnosis and intervention of severe sepsis.This study investigated the relationship between melatonin secretion and outcome of sepsis after 28-day admission.Methods:Patients with sepsis were randomly divided into an eye mask group and a control group.Blood and urine samples were collected from day 0 to 4.Relevant clinical data and 28-day survival data were obtained.Serum melatonin and urine 6-hydroxy sulfate melatonin(6-SMT)levels were measured.Results:The outcome of sepsis did not differ between the eye mask and control groups.Acute Physiology and Chronic Health Evaluation(APACHE)II and Sequential Organ Failure Assessment(SOFA)scores were significantly higher and monocyte human leukocyte antigen-DR(mHLA-DR),serum melatonin,nocturnal urine 6-SMT,and 24-hour urine 6-SMT levels were significantly lower in the nonsurvivors than in the survivors.The outcome at day 28 after admission was significantly associated with APACHE II and SOFA scores and mHLA-DR,nocturnal urine 6-SMT,and 24-hour urine 6-SMT levels.The areas under the receiver operating characteristic curve were 0.785,0.740,0.774,and 0.858 for APACHE II score,SOFA score,mHLA-DR expression,and nocturnal urine 6-SMT amount,respectively.The optimal thresholds for mHLA-DR and nocturnal urine 6-SMT were 30.13%and 43.60%,respectively.Nocturnal urine 6-SMT level was significantly and positively correlated with mHLA-DR expression.Conclusion:Nocturnal urine 6-SMT level may be a feasible biomarker to predict the outcome of patients with sepsis.The use of a night-time eye mask has no significant effect on the outcome of sepsis.Clinical trials:This study was registered at clinicaltrials.gov(NCT02304224). | Wenjie Li Jiameng Chen Shuming Pan Chengjin Gao | 2022 | Emergency and Critical Care Medicine2022，2，3： | 0 |
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