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您的检索式：作者名="PENGLEE Rachit"
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| 1 | 斑马鱼nos2a基因的生长调控作用显示文摘为了在斑马鱼中探索诱导型一氧化氮合酶(Nos2)在生长调控中的作用,通过CRISPR/Cas9基因编辑技术,首次构建了可稳定遗传的nos2a缺失型(nos2a^(-/-))斑马鱼家系。通过监测其生长过程发现,nos2a^(-/-)斑马鱼的整体生长趋势较nos2a正常型(nos2a^(+/+))斑马鱼偏低,且nos2a^(-/-)成鱼的体型显著小于同期的nos2a^(+/+)斑马鱼(P<0.01):nos2a^(+/+)成鱼体长(29.53±0.60)mm,nos2a^(-/-)成鱼体长(26.34±0.69)mm;nos2a^(+/+)成鱼体质量(512.80±30.75)mg,nos2a^(-/-)成鱼体质量(394.40±16.91)mg;而在肌纤维密度(P=0.718)及躯椎骨的横截面积上(P=0.548)两者没有显著差异。qPCR检测发现,出膜8 d时,nos2a^(-/-)斑马鱼的生长激素基因gh1的mRNA水平(P<0.01)及类胰岛素生长因子基因igf1的mRNA水平(P<0.001)均显著低于nos2a^(+/+)斑马鱼;出膜68 d时,nos2a^(-/-)斑马鱼的gh1(P<0.0001)和igf1(P<0.0001)的mRNA水平同样显著低于nos2a^(+/+)斑马鱼。以上结果表明,斑马鱼中nos2a的功能缺失导致鱼体生长受到抑制,而gh1及igf1 mRNA的水平下调是其潜在原因。 | 高磊 黄亚娟 PENGLEE Rachit 易欣鑫 鲍宝龙 | 2021 | 上海海洋大学学报2021，30，4： | 4 |
| 2 | The role of nitric oxide and neuronal nitric oxide synthase in zebrafish(Danio rerio)shoaling显示文摘Nitric oxide(NO)-the product of arginine metabolism catalyzed by nitric oxide synthases(NOS)-is a well-known neurotransmitter which plays an important role in metabolism and amino acid transportation in the nervous system.In particular,it can inhibit monoamine neurotransmitter transportation which affects animal behavior,especially social behavior.Shoaling-is a one kind of social behavior.It is a behavior that individual fish choose to join with their group within two factors;food and predation risk.Shoaling fish has quickly responded to predator and increased the change in feeding competition.In addition,shoaling also effect to stress response on stock density of aquaculture system.The effect of NO molecular signaling on the dopamine pathway was investigated using zebrafish(Danio rerio)as a model organism.Our aim was to understand the role of NOS and NO in shoaling behavior,which is typical of zebrafish.The concentration of NO in the zebrafish brain was modulated using a knockout for the neuronal NOS gene,and NO production was induced through treatment with L-arginine.The existence of NO in the zebrafish brain was confirmed by using a fluorescent probe.Dopamine concentration in the brain was measured by UPLC tandem mass spectrometer.We measured shoaling cohesion of all individual fish of D.rerio,using average distance between all pairs of fish(nearest neighbor distance)and analyzed tracking by Zebralab ViewPoint software.Collectively,our results suggest that a lower level of NO was associated with a higher level of dopamine,which in turn leads to the shoaling behavior. | Rachit Penglee Lei Gao Yajuan Huang Liping Liu Sukkrit Nimitkul Baolong Bao | 2021 | Aquaculture and Fisheries2021，6，6： | 0 |
| 3 | aguA基因缺失对嗜水气单胞菌致病性的影响显示文摘嗜水气单胞菌(Aeromonas hydrophila)是水环境常见的病原体,具有较强致病性。在本研究中,首次敲除了嗜水气单胞菌aguA基因,通过转录组分析发现aguA基因敲除后细菌的黏附、致病、胺代谢等生物过程下降。通过qRT-PCR进一步验证发现10个黏附基因、9个发病基因和8个胺代谢基因的mRNA表达水平明显下降;此外,转录组水平分析结合qRT-PCR验证发现Ⅵ型分泌系统基因、Ⅱ型分泌系统基因、鞭毛合成相关基因、菌毛合成相关基因、溶血基因、Ⅰ型分泌系统基因、肠毒素基因、RTX基因等毒力基因的RNA表达水平也出现下调。与野生菌株(WT)相比,aguA敲除株(ΔaguA)感染金鱼的致病力降低了86.6%,且aguA敲除株生物膜形成能力降低了16.6%。以上结果显示,aguA基因与嗜水气单胞菌的致病性密切相关。 | 赵东康 Rachit PENGLEE 车金远 易欣鑫 邱超达 鲍宝龙 | 2023 | 上海海洋大学学报2023，32，3： | 0 |
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