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| 1 | Association between low molecular polypeptide 7 single nucleotide polymorphism and response to therapy in hepatitis C virus infection显示文摘AIM: To investigate the relationship between low molecular polypeptide-7 (LMP-7) gene polymorphism and response to interferon (IFN) therapy in chronic hepatitis C virus (HCV) patients. METHODS: LMP-7 polymorphism at codon 49 with nucleotide substitution from A to C was amplified in 104 chronic HCV patients of genotype 4. The amplicons were digested with restriction endonuclease Bsm I and the produced restriction fragment length polymorphism was analyzed. Patients received IFN + regional blood volume therapy for 48 wk and the frequency of thissingle nucleotide polymorphism (SNP) was statistically correlated with treatment response. The exclusion criteria for these patients were stated by the national health program for treating viral hepatitis. Main exclusion criteria included co-infection with hepatitis B virus or schistosomiasis, thyroid dysfunction, uncontrolled diabetes mellitus, history of long term drug or alcohol intake and autoimmune hepatitis. Multivariate analyses were done to correlate LMP-7 SNP plus several factors such as age, gender, weight, serum alpha-fetoprotein (AFP) and alanine aminotransferase levels, liver activity, fibrosis score and viral load with response to therapy. RESULTS: The data presented in this study clearly demonstrated statistically significant differences between sustained virological response (SVR) (defined as the absence of HCV RNA levels in the patient's sera at least 6 mo after discontinuation of treatment) and non-response (NR) (where HCV RNA levels in the patient's sera never become undetectable for 6 mo during or after treatment). Variables were described as odds ratio with 95%CI. The data were considered significant if P values were ≤ 0.05; highly significant if P < 0.01 and very highly significant if P < 0.001. Current data showed that 91.7% of patients carrying LMP-7 C/C allele were associated with SVR, while the other two genotypes C/A and A/A were associated with NR patients, 83.3% and 64.3% respectively, showing that genotype CC was strongly associated with response to interferon (95%CI: 12.0719-134.6572, P = 0.0001). The majority of parameters recorded in SVR and NR patients included higher values of mean age (P = 0.004), alanine aminotransferase (P = 0.001), AFP (P = 0.001), body weight (P = 0.025), viral load (P = 0.025), higher fibrosis and histological activity index indices among NR vs SVR patients. Also, the multivariate statistical analysis of the different factors of fibro-sis score, liver activity grade, genotypes and alleles of LMP-7 gene polymorphism in responders and NRs of HCV patients in this study showed that HCV patients with A allele had a very highly significant association with the NRs, high fibrosis and higher liver activity, while the C allele had a very highly significant association with the responders, low fibrosis and lower liver activity (95%CI: 3.5800-13.2519, P = 0.0001).CONCLUSION: LMP-7 SNP is a candidate gene that should be considered when designing a mathematical model for predicting response to therapy and disease progression in HCV patients. | Moataza H Omran Basma E Fotouh Samar S Youssef Noha E Ibrahim Wael Nabil EL-Sayed M Mahdy Wafaa G Shosha Mostafa K El-Awady | 2013 | World Journal of Hepatology2013，5，3： | 4 |
| 2 | Differential expression of cell cycle regulators in HCV-infection and related hepatocellular carcinoma显示文摘AIM:To investigate cell cycle proteins in chronic hepatitis C virus infection in order to analyze their role in the process of hepatocyte transformation and to characterize their prognostic properties. METHODS:Subjects of the current study included 50 cases of chronic hepatitis C(CHC) without cirrhosis,30 cases of CHC with liver cirrhosis(LC) ,and 30 cases of hepatitis C-related hepatocellular carcinoma(HCC) admitted to the Department of Hepato-Gastroenterology,Theodor Bilharz Research Institute(TBRI) ,Giza,Egypt.Fifteen wedge liver biopsies,taken during laparoscopic cholecystectomy,were also included as normal controls.Laboratory investigations including urine and stool analysis,liver function tests and prothrombin concentration;serologic markers for viral hepatitis and ultrasonography were done for all cases of the study together with immunohistochemical analysis using primary antibodies against Cyclin D1,Cyclin E,p21,p27 and Rb/p105 proteins. RESULTS:Normal wedge liver biopsies didn't express Cyclin E or Rb/p105 immunostaining but show positive staining for Cyclin D1,p21 and p27.Cyclin D1 expressed nuclear staining that was sequentially increased from CHC to LC(P<0.01) to HCC(P<0.001) cases;meanwhile,Cyclin E revealed nuclear positivity only in the case of HCCs patients that was directly correlated to Rb/p105 immuno-reactivity.The expression of p21 and p27 was significantly increased in CHC and LC cases compared to normal controls and HCCs with no significant difference between well-and poorlydifferentiated tumors.p21 showed only a nuclear pattern of staining,while,p27 presented with either cytoplasmic and/or nuclear reactivity in all studied cases.Correlation analysis revealed a direct relation between Cyclin D1 and p21 in CHC cases(P<0.001) ,between Cyclin D1 and Cyclin E in HCCs(P<0.01);however,an inverserelationship was detected between Cyclin D1 and p21 or p27(P<0.001) and between p21 and Rb/p105(P<0.05) in HCCs. CONCLUSION:Upregulation of Cyclin D1 in CHC plays a vital role in the development and differentiation of HCC;while,Cyclin E may be a useful marker for monitoring tumor behavior.p21 and p27 can be used as predictive markers for HCC.Furthermore,higher expression of Rb/p105 as well as inverse relation with p21 and histologic grades suggests its important role in hepatic carcinogenesis. | Azza E El Bassiouny Mona M Nosseir Mona K Zoheiry Noha A Ameen Ahmed M Abdel-Hadi Ibrahim M Ibrahim Suher Zada Abdel-Hakeem Saad El-Deen Nora E El-Bassiouni | 2010 | World Journal of Hepatology2010，2，1： | 3 |
| 3 | Comparison of methods to detect biosurfactant production by diverse microorganisms 显示文摘 | NOHA H Y KATHLEEN E | 2004 | Microbiological Methods2004，56，： | 1 |
| 4 | Are the outcomes of clinical pathways evidence-based ? A critical app raisal of clinical pathway evaluation research显示文摘 | Noha E Baz MSc Berrie Middel PhD | 2007 | Evaluation in Clinical Practice2007，76，8： | 1 |
| 5 | Are the outcomes of clinical pathways evidence-based:a critical appraisal of clinical pathway evaluation research显示文摘 | Noha E Berrie Middel Jitse P van Dijk MD | 2007 | J Eval Clin Pract2007，13，6： | 1 |
| 6 | Continuous positive airway pressure for treatment of postoperative hypoxemia:a randomized controlled trial显示文摘 | Squadrone V Noha M Ceruttl E | 2005 | JAMA2005，293，5： | 1 |
| 7 | Comparison of methods to detect biosurfactant production by diverse microorganisms显示文摘 | Noha H Y Kathleen E D David P N | 2004 | Journal of Microbiological Methods2004，56，： | 1 |
| 8 | Are the outcomes of clinical pathways evidence-based:a critical appraisal of clinical pathway evaluation research显示文摘 | Noha E Berrie Middel Jitse P van Dijk MD | 2007 | J Eval Clin Pract2007，13，6： | 1 |
| 9 | Continuous positive airway pressure for treatment of postoperative hypoxemia; A randomized controlled trial显示文摘 | Squadrone V Noha M Ceruttl E | 2005 | JAMA2005，293，5： | 1 |
| 10 | Comparison of methods to detect biosurfactant production by diverse microorganisms显示文摘 | Noha H Y Kathleen E | 2004 | Microbiological Methods2004，56，： | 1 |
| 11 | 查看详情显示文摘 | H Y Noha E D Kathleen J M Michael | | 0，，： | 1 |
| 12 | Comparison of methods to detect biosurfactant production by diverse microorganisms显示文摘 | Noha H Youssef Kathleen E Duncan David ENagle | 2004 | Microbiological Methods2004，56，： | 1 |
| 13 | Coagulation of soymilk and quality of tofu as affected by freeze treatment of soybeans显示文摘 | Noha E J Parkb S Y Pakc J I | 2005 | Food Chemistry2005，91，4： | 1 |
| 14 | Coagulation of soymilk and quality of tofu as affected by freeze treatment of soybeans显示文摘 | NOHA E J PARKB S Y PAKC J I | 2005 | Food Chemistry2005，91，4： | 1 |
| 15 | Continuous positive airway pressure for treatment of postoperative hypoxemia: A random- ized controlled trial显示文摘 | Squadrone V Noha M Ceruttl E | 2005 | JAMA2005，293，： | 1 |
| 16 | Permutation-substitution image encryption scheme based on a modified chaotic map in transform domain显示文摘A new chaotic image encryption scheme based on permutation and substitution in the Fourier domain is presented.Fractional Fourier Transform(FRFT)is used before the encryption scheme to get a large degree of randomization.The permutation is achieved by Baker map and the substitution by a key-related-to-plain-image algorithm based on the modified Logistic map.Modification of the Logistic map is developed to increase the space of the encryption key,and hence increase the security.The key of the encryption algorithm dependents on the plain image,and thus,the cipher image is sensitive to both the initial key and the plain image to resist known-plaintext and chosen plaintext attacks.The key space is large and hence the algorithm can effectively resist brute-force attacks.The proposed scheme is examined using different performance evaluation metrics and the results prove that the proposed scheme is highly secure,and it can effectively resist different attacks. | Ramadan Noha Ahmed HossamEldin H El-khamy Said E Abd El-Samie Fathi E | 2017 | Journal of Central South University2017，24，9： | 1 |
| 17 | Spinal accessory neuropathy in patients with chronic neck pain显示文摘AIM: To assess the presence of spinal accessory neuropathy in patients with chronic neck pain.METHODS: Patients with pain either regional or focal in the neck or shoulders for at least 6 mo(chronic neck pain) were recruited randomly from the Rheumatology and Rehabilitation Outpatient Clinic at the Faculty of Medicine-Suez Canal University. Two groups were compared: 30 patients with chronic neck pain with mean age(36.97 ± 12.45 years) and 10 apparently healthy controls. Trapezius muscle examination including inspection and range of motion both active and passive was performed. A full clinical neurological examination was carried out to exclude peripheral neuropathy and motor neuron disease. According to the subject's type of work, cases were categorized into labor-intensive and non-labor intensive tasks. A nerve conduction study(NCS) was performed on spinal accessory nerves at both sides for all patients and controls. Parameters including latencies and amplitudes of compound motor action potential(CMAP) were compared with the chronicity of neck pain using the neck disability score. This cross sectional study was carried in the Rheumatology and Rehabilitation Department, at Suez Canal University Hospital, Ismailia, Egypt.RESULTS: Physical examination revealed that 80% of cases had spinal trapezius muscle spasm. Restricted neck motion was present in 16.6% of cases. No one suffered from muscle wasting or weakness. Pain was bilateral in 18 patients(60%), localized to the right side in six patients(20%) and localized to the left side in six patients(20%). The causes of neck pain in the patients studied were nonspecific, due to physical stresses, cervical spondylosis and mild cervical disc herniation. Mean disease duration in patients with labor-intensive tasks was(3.9 ± 2.1 years), which was longer than that in patients with non-labor intensive tasks(3.1 ± 1.9 years); however, this difference was statistically insignificant. Spinal accessory NCSs were performed while subjects were in sitting positions and relaxed with naturally suspended arms to minimize muscular movement. The results of electrophysiological studies revealed that mean right and left latencies of the spinal accessory nerve were 2.96 ± 0.69 ms, 2.98 ± 0.61 ms in the patient group and 2.44 ± 0.38 ms, 2.33 ± 0.36 ms in control group respectively. These differences were statistically significant with P = 0.028 and 0.006 respectively. Spinal accessory NCS showed normal CMAP amplitude in both patients and controls. Comparing the results of the neck disability index(NDI) to different characteristics in patients with chronic neck pain, showed that patients with labor-intensive work had a higher NDI score mean(34.7 ± 9.5) compared to those with non-labor-intensive work, with significant statistical difference(P = 0.011). In addition, mean NDI scores were higher in males, and patients aged over 40 years and this difference was statistically significant(P = 0.007 and P = 0.009 respectively). Correlation studies between right and left spinal accessory nerve latencies and disability percent calculated using the NDI revealed a positive correlation. Moreover, there was a positive correlation between age and disability percent.CONCLUSION: This study demonstrates electrophysiological evidence of demyelination in a significant proportion of patients with chronic cervical pain. | Mohamed A Hefny Mona S Ghaly Sahar M Greish Noha M Abogresha Ayman E Fahim | 2012 | World Journal of Rheumatology2012，2，2： | 1 |
| 18 | Comparison of methods to detect biosurfactant production by diverse microorganisms显示文摘 | NOHA H Y KATHLEEN E D ROY M K | 2004 | Journal of Microbiological Methods2004，56，： | 1 |
| 19 | Comparison of methods to detect biosurfactant production by diverse microor- ganisms 显示文摘 | NOHA H Y KATHLEEN E DAVID P N | 2004 | J Microbiol Methods2004，56，3： | 1 |
| 20 | Applicability and efficacy of a model for prevention of perinatal transmission of hepatitis B virus infection:Single center study in Egypt显示文摘AIM:To identify possible maternal risk factors for hepatitis B virus(HBV)acquisition and assess the efficacy of immunoprophylaxis given to infants born to hepatitis B virus surface antigen(HBs Ag)positive mothers.METHODS:Screening of 2000 pregnant females wascarried out using rapid test and confirmed by enzyme immunoassay.A questionnaire consisting of 20 questions about the possible risk factors for acquisition of HBV infection was filled for every pregnant HBs Ag positive female in addition to at least 2 pregnant HBs Ag negative females for each positive case.Infants of HBs Ag positive women were offered passive and active immunoprophylaxis within the 1st 48 h after birth,in addition to 2nd and 3rd doses of HBV vaccine after1 and 6 mo respectively.Infants were tested for HBs Ag and hepatitis B surface antibodies(HBs Ab)at six months of age.RESULTS:HBs Ag was confirmed positive in 1.2%of tested pregnant women.Risk factors significantly associated with HBV positivity were;history of injections(OR=5.65),history of seeking medical advice in a clinic(OR=7.02),history of hospitalization(OR=6.82),history of surgery(OR=4)and family history of hepatitis(OR=3.89)(P<0.05).Dropout rate was 28%for HBs Ag women whose rapid test was not confirmed and could not be reached to provide immunoprophylaxis for thier newborns.Immunoprophylaxis failure was detected in only one newborn(3.7%)who tested positive for HBs Ag at 6 mo of age;and vaccine failure(seronegative to HBs Ab after 4 doses of the vaccine)was detected in another one(3.7%).The success rate of the immunoprophylaxis regimen was 92.6%.CONCLUSION:This pilot study shows that a successful national program for prevention of perinatal transmission of HBV needs to be preceded by an awareness campaign to avoid a high dropout rate. | Hanaa M El-Karaksy Lamiaa M Mohsen Doa'a A Saleh Mona S Hamdy Noha A Yassin Mohamed Farouk Mohamed E Salit Mortada H El-Shabrawi | 2014 | World Journal of Gastroenterology2014，20，45： | 1 |
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