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| 1 | Correlation between rpoB gene mutation in Mycobacterium avium subspecies paratuberculosis and clinical rifabutin and rifampicin resistance for treatment of Crohn’s disease显示文摘AIM: To investigate overlapping regions of the rpoB gene previously involved with rifamycin resistance in M. tuberculosis and seek correlation between rpoB mutations in clinical MAP strains with susceptibility to RIF and RFB. METHODS: We designed a molecular-based PCR method for the evaluation of rifabutin (RFB) and rifampicin (RIF) resistance based on probable determinant regions within the rpoB gene of MAP, including the 81 bp variable site located between nucleotides 1363 and 1443. The minimum inhibitory concentration (MIC) for RIF was also determined against 11 MAP isolates in attempt to seek correlation with rpoB sequences. RESULTS: We determined that MAP strain 18 had an MIC of > 30 mg/L and ≤ 5 mg/L for RIF and RFB respectively, and a significant and novel rpoB mutation C1367T, compared to an MIC of ≤ 1.0 mg/L for both drugs in the wild type MAP. The 30-fold increase in the MIC was a direct result of the rpoB mutation C1367T, which caused an amino acid change Thr456 to Ile456 in the drug’s binding site. In addition, MAP strain 185 contained five silent rpoB mutations and exhibited an MIC comparable to the wild-type. Moreover, our in vitroselected mutation in MAP strain UCF5 resulted in the generation of a new resistant strain (UCF5-RIF16r) that possessed T1442C rpoB mutation and an MIC > 30 mg/L and > 10 mg/L for RIF and RFB respectively. Sequencing of the entire rpoB gene in MAP strains UCF4, 18, and UCF5-RIF16r revealed an rpoB mutation A2284C further downstream of the 81 bp variable region in UCF4, accounting for observed slight increase in MIC. In addition, no other significant mutations were found in strains 18 and UCF-RIF16r. CONCLUSION: The data clearly illustrates that clinical and in vitro-selected MAP mutants with rpoB mutations result in resistance to RIF and RFB, and that a single amino acid change in the beta subunit may have a significant impact on RIF resistance. Unconventional drug susceptibility testing such as our molecular approach will be beneficial for evaluation of antibiotic effectiveness. This molecular approach may also serve as a model for other drugs used for treatment of MAP infections. | Daniel R Beckler Sammer Elwasila George Ghobrial John F Valentine Saleh A Naser | 2008 | World Journal of Gastroenterology2008，14，17： | 2 |
| 2 | Deep Nondestructive testing using a bulk high T,rf-SQUID显示文摘 | Naser S Alzayed Fan Changxin Lu D F | 1994 | Ieee Transaction on Applied Superconductivity1994，4，2： | 1 |
| 3 | Urodynamic study in women with pure stress urinary incontinence显示文摘 | Valdevenito JP Aguila F Naser M | 2015 | Actas Urol Esp2015，39，2： | 1 |
| 4 | Valproate, weight gain and carbohydrate craving : A gender study 显示文摘 | El-Khatib F Rauchenzauner M Lechleitner M Hoppichlcr F Naser A Waldmann M | 2007 | Seizure2007，16，3： | 1 |
| 5 | CPW-fed compact slot antenna for WLAN operation in a laptop computer 显示文摘 | Naser M M Sadeghi F R Danideh A | 2010 | Microwave and Optical Technology Letters2010，52，6： | 1 |
| 6 | A chaotic secure communication scheme using fractional chaotic systems based on an extended fractional Kalman filter显示文摘 | B A Kiani F Kia P Naser | 2009 | Communications in Nonlinear Science and Numerical Simulation2009，14，3： | 1 |
| 7 | Application of multilocus sequence analysis(MLSA) for rapid identification of Enterococcus species based on rpoA and pheS genes显示文摘 | NASER S M THOMPSON F L HOSTE B | 2005 | Microbiology2005，151，： | 1 |
| 8 | Protective and susceptible HLA Class I gene in patients with End-stage renal failure 显示文摘 | Peyman PD Naser F Mohammad N | 2008 | Res J Biol Sci2008，3，11： | 1 |
| 9 | Deep Norldestructive Testing using a Bulk High Tc rf SQUID显示文摘 | Naser S A Chang xin Fan Lu D F | 1994 | IEEE Transactions on Applied Superconductivity1994，4，2： | 1 |
| 10 | Numerical simulations of the reactive mixing in a commercially operated stirred ethoxylation reactor 显示文摘 | Chiu Y N Naser J Ngian K F | 2008 | Chemical Engineering Science2008，63，： | 1 |
| 11 | Computational fluid dynamics study of vehicle A-pillar aero-acoustics 显示文摘 | Murad N Naser J Alam F | 2013 | Applied Acoustics2013，74，： | 1 |
| 12 | Phylogeny and identi- fication of enterococci by atpA gene sequence analysis显示文摘 | NASER S THOMPSON F L HOSTE B | 2005 | Journal of Clinical Microbiology2005，43，5： | 1 |
| 13 | Protective and Susceptible HLA Class I Genes in Patients with End-Stage Renal Disease 显示文摘 | Peyman PD Naser F Mohammad N | 2008 | Res J Biosci2008，3，11： | 1 |
| 14 | Quadratic stabilization of counties time systems with state-delay and norm-bounded time-varying uncertainties显示文摘 | MAGDI S MAHMOUD NASER F AL MUTHAIRI | 1994 | IEEE Trans on Auto Control1994，39，10： | 1 |
| 15 | A chaotic secure communication scheme using fractional chaotic systems based on an extended fractional Kalman filter 显示文摘 | Arman K B Kia F Naser P | 2009 | Communications in Nonlinear Science and Numerical Simulation2009，14，3： | 1 |
| 16 | Culture of Mycobacterium avium subspecies paratuberculosis from the blood of patients with Crohn’s disease显示文摘 | Saleh A Naser George Ghobrial Claudia Romero John F Valentine | 2004 | The Lancet2004，，9439： | 1 |
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