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| 1 | 定点突变提高过氧化氢酶热稳定性和催化效率显示文摘过氧化氢酶催化过氧化氢分解形成水和氧,主要作用是保护细胞免受活性氧(ROS)的损伤,尽管其在工业上被广泛使用,但因热稳定的不足限制了它的使用前景。本研究以Bacillus pumilus ML413来源的血红素过氧化氢酶(KatX2)为研究对象,采用定点突变技术提高其热稳定性及催化效率。利用PoPMuSiC algorithm软件计算并预测了其潜在突变位点的折叠自由能,根据预测的结果,选择了其中自由能最低的3个位点(K117V,K117I和K117M)进行突变。结果发现,对K117位点进行突变,可以有效提高KatX2的热稳定性,突变体K117V在60℃下半衰期比野生型KatX2提高38 min,催化效率较突变前提高31.7%。根据结构分析发现,该点突变并未改变KatX2的二级结构。突变体K117V具有良好的工业应用潜力。 | MUGISHA Samson 杨套伟 徐美娟 张显 ULIHO Alphonse 钱海峰 王立 饶志明 | 2020 | 食品与生物技术学报2020，39，3： | 2 |
| 2 | Annual trends of human brucellosis in pastoralist communities of south-western Uganda: a retrospective ten-year study显示文摘Background:Human brucellosis is prevalent in both rural and urban Uganda,yet most cases of the disease in humans go unnoticed and untreated because of inaccurate diagnosis,which is often due to the disease not manifesting in any symptoms.This study was undertaken to describe trends in laboratory-confirmed human brucellosis cases at three health facilities in pastoralist communities in South-western,Uganda.Methods:Data were collected retrospectively to describe trends of brucellosis over a 10-year period(2003–2012),and supplemented with a prospective study,which was conducted from January to December 2013.Two public health facilities and a private clinic that have diagnostic laboratories were selected for these studies.Annual prevalence was calculated and linearly plotted to observe trends of the disease at the health facilities.A modified Poisson regression model was used to estimate the risk ratio(RR)and 95%confidence intervals(CIs)to determine the association between brucellosis and independent variables using the robust error variance.Results:A total of 9,177 persons with suspected brucellosis were identified in the retrospective study,of which 1,318(14.4%)were confirmed cases.Brucellosis cases peaked during the months of April and June,as observed in nearly all of the years of the study,while the most noticeable annual increase(11–23%)was observed from 2010 to 2012.In the prospective study,there were 610 suspected patients at two public health facilities.Of these,194(31.8%)were positive for brucellosis.Respondents aged 45–60 years(RR=0.50;CI:0.29–0.84)and those that tested positive for typhoid(RR=0.68;CI:0.52–0.89)were less likely to have brucellosis.Conclusions:With the noticeable increase in prevalence from 2010 to 2012,diagnosis of both brucellosis and typhoid is important for early detection,and for raising public awareness on methods for preventing brucellosis in this setting. | Catherine Kansiime Elizeus Rutebemberwa Benon BAsiimwe Fredrick Makumbi Joel Bazira Anthony Mugisha | 2015 | Infectious Diseases of Poverty2015，4，1： | 2 |
| 3 | Economic viability of biogas energy production from family-sized digesters in U- ganda显示文摘 | P N Walekhwa D Lars J Mugisha | 2014 | Biomass and Bioenergy2014，70，0： | 1 |
| 4 | The health systemcost of post - abortion care in Uganda 显示文摘 | Vlassoff M Mugisha F Sundaram A | 2014 | Health Policy andPlanning2014，29，1： | 1 |
| 5 | Health sys- tem barriers affecting the implementation of collaborative TB -HIV services in Uganda 显示文摘 | Okot-Chono R Mugisha F Adatu F | 2009 | Int J Tubere Lung Dis2009，13，： | 1 |
| 6 | The health system cost of post-abortion care in Uganda显示文摘 | Vlassoff M Mugisha F Sundaram A | 2012 | Health Policy Plan2012，29，1： | 1 |
| 7 | The Integration of Field Survey and Remote Sensing for Biodiversity Assessment: A Case Study in the Tropical Forests and Wetlands of Sango Bay,Uganda显示文摘 | Fuller R M Groom G B Mugisha S | 1998 | Biological Conservation1998，86，3： | 1 |
| 8 | Determinants of mortality and loss to follow-up among adults enrolled in HIV care services in Rwanda显示文摘 | Mugisha V Teasdale CA Wang C | 2014 | PLoS One2014，9，85： | 1 |
| 9 | Doing qualitative research on suicide in a developing country 显示文摘 | Mugisha J Knizek BL Kinyanda E | 2011 | Crsis2011，32，1： | 1 |
| 10 | Mathematical analysis of the dynamics of visceral leishmaniasis in the Sudan显示文摘 | Elmojtaba I M Mugisha J Y T Hashim M H A | | 0，，： | 1 |
| 11 | The integration of field survey and remote sensing for biodiversity assessment: a case study in the tropical forests and wetlands of Sango Bay, Uganda显示文摘 | Fuller R M Groom G B Mugisha S | 1998 | Biological conservation1998，86，3： | 1 |
| 12 | Willingness to pay for sound waste management in urban and peri-urban areas of the Lake Victoria crescent region Uganda 显示文摘 | Ekere W Mugisha J Drake L | 2010 | International Journal of Muhidisciplinary Research2010，，2： | 1 |
| 13 | Knowledge and perceptions of brucellosis in the pastoral communities adjacent to Lake Mburo National Park, Uganda 显示文摘 | KANSIIME C MUGISHA A MAKUMBI F | 2014 | BMC Public Health2014，14，2： | 1 |
| 14 | Comparison of Single-Stage and Staged Progression Models for HIV/AIDS Transmission 显示文摘 | Baryarama F Mugisha JY | 2007 | International Journal of Mathematics and Mathematical Sciences2007，10，： | 1 |
| 15 | A mathematical model for the dynamics of HIV/AIDS with gradual behaviour change显示文摘 | Mugisha JYT Luboobi LS | 2006 | Computational and Mathematical Methods in Medicine2006，7，1： | 1 |
| 16 | Mathematical analysis of the dynamics of visceral leishmaniasis in the Sudan显示文摘 | ELmojtaba I M Mugisha J Y T Hashim M H A | 2010 | Applied Mathematics and Computation2010，217，6： | 1 |
| 17 | winingnessto-pay for community based insurance in Burkina Faso 显示文摘 | Dong H Kouyate B Calms J Mugisha F Sanerbom R | 2003 | Health Economics2003，12，10： | 1 |
| 18 | Oral Manifestations in HIV/AIDS - Infected Chil- dren显示文摘 | Charles Mugisha Rwenyonyi Annet Kutesa Louis Muwa- zi | 2011 | Eur J Dent2011，5，3： | 1 |
| 19 | Reliability of a perinatal out- comes measure: the optimality index- US 显示文摘 | Seng JS Mugisha E Miller JM | 2008 | Midwifery Womens Health2008，53，2： | 1 |
| 20 | Knowledge and perceptions of brucellosis in the pastoral communities adjacent to Lake Mburo Na- tional Park, Uganda显示文摘 | Kansiime C Mugisha A Makumbi F | 2014 | BMC Pub Health2014，14，： | 1 |
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