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| 1 | YKL40 expression in CD14^+ liver cells in acute and chronic injury显示文摘AIM:To demonstrate that CD14 + cells are an important source of the growth factor YKL40 in acute and chronic liver damage.METHODS:Rats were inoculated with one dose of CCl4 to induce acute damage.Liver biopsies were obtained at 0,6,12,24,48 and 72 h.For chronic damage,CCl4 was administered three days per week for 6 or 8 wk.Tissue samples were collected,and cellular populations were isolated by liver digestion and purified by cell sorting.YKL40 mRNA and protein expression were evaluated by realtime polymerase chain reaction and western blot.RESULTS:Acute liver damage induced a rapid increase of YKL40 mRNA beginning at 12 h.Expression peaked at 24 h,with a 26fold increase over basal levels.By 72 h however,YKL40 expression levels had nearly returned to control levels.On the other hand,chronic damage induced a sustained increase in YKL40 expression,with 7and 9fold higher levels at 6 and 8 wk,respectively.The pattern of YKL40 expression in different subpopulations showed that CD14+cells,which include Kupffer cells,are a source of YKL40 after acute damage at 72 h[0.09 relative expression units(REU)]as well as after chronic injury at 6 wk(0.11 REU).Hepatocytes,in turn,accounted for 0.06 and 0.01 REU after 72 h(acute)or 6 wk(chronic),respectively.The rest of the CD14cells(including T lymphocytes,B lymphocytes,natural killer and natural killer T cells) yielded 0.07 and 0.15 REU at 72 h and 6 wk,respectively.YKL40 protein expression in liver was detected at 72 h as well as 6 and 8 wk,with the highest expression relative to controls(11fold;P≤0.05)seen at 6 wk.Macrophages were stimulated by lipopolysaccharide.We demonstrate that under these conditions,these cells showed maximum expression of YKL40 at 12 h,with P<0.05 compared with controls.CONCLUSION:Hepatic CD14 + cells are an YKL40 mRNA and protein source in acute and chronic liver injury,with expression patterns similar to growth factors implicated in inflammationfibrogenesis. | Oscar Pizano-Martínez Irinea Yaez-Sánchez Pilar Alatorre-Carranza Alejandra Miranda-Díaz Pablo C Ortiz-Lazareno Trinidad García-Iglesias Adrian Daneri-Navarro Mónica Vázquez-Del Mercado Mary Fafutis-Morris Vidal Delgado-Rizo | 2011 | World Journal of Gastroenterology2011，17，33： | 3 |
| 2 | Effect of simultane-ous down-regulation of pectate lyase and endo-(3-l,4-glucanasegenes on strawberry fruit softening显示文摘 | YOUSSEF S M IRAIDA A L^PEZ-ARANDA J M’ RAFAELS VICTORIANO V GIORGIO C ROSARIO B-P FERNAN-DO P-A OUESADA M A MERCADO J A | 2013 | Molecular Breeding2013，31，： | 1 |
| 3 | Effect of salinity acclimation on oxygen consumption of juvenile of the white shrimp Litopenaeus vannamei显示文摘 | ROSAS C LOPEZ N MERCADO P | 2001 | Journal of Crustacean Biology2001，21，4： | 1 |
| 4 | Early and late fistula failure显示文摘 | Mercado C Salman L Krishnamurthy G | 2008 | Clin Nephrol2008，69，2： | 1 |
| 5 | Surgical treatment of non-cirrhotic preinusoidal portal hypertension 显示文摘 | Mercado MA Orozco H Chan C | 2004 | Hepatogas-troenterology2004，51，60： | 1 |
| 6 | The heat-shock protein 90 inhibitor 17-allylamino-17-demethoxygeldanamycin suppresses glial inflammatory responses and ameliorates experimental autoimmune encephalomyelitis显示文摘 | Dello Russo C Polak PE Mercado PR | 2006 | J Neurochem2006，99，5： | 1 |
| 7 | Nucleotide sequence analysis of genome segment A of infectious bursal disease Virus显示文摘 | Jack wood D J Mercado C C | 1990 | J Gen Virol1990，71，： | 1 |
| 8 | Nucleotide sequence analysis of genome segment A of infectious bursal disease virus显示文摘 | KIBENGE F S JACKWOOD D J MERCADO C C | 1990 | J Gen Virol1990，71，3： | 1 |
| 9 | lhylogenet- ie analysis of Verticillium dahliae vegetative compatibility grounps 显示文摘 | Collado R M Mercado B J Olivares G C | 2008 | Phytopathology2008，98，9： | 1 |
| 10 | Quality of care in advanced ovarian cancer : the importance of provider specialty显示文摘 | Mercado C Zingmond D Karlan B Y | 2010 | Gynecol Oncol2010，117，1： | 1 |
| 11 | Largethermoplastic parts quality improvements using moni-torized nozzle显示文摘 | Fernandez A Mercado D Javierre C | 2008 | Strojniski Vestnik-Journal ofMechanical Engineering2008，54，4： | 1 |
| 12 | Surgical treatment of non-cirrhotie presinusoidal portal hypertension 显示文摘 | Mercado M A Orozco H Chan C | 2004 | Hepatogastroenterology2004，51，60： | 1 |
| 13 | Effect of biosynthetic intermediates and citrate on the phenyllactic and hydroxyphenyllactic acids production by Lactobacillus plantarum CRL778显示文摘 | Dallagnol A Catalán C Mercado M | 2011 | Journal of Applied Microbiology2011，111，6： | 1 |
| 14 | Acute bile duct injury, the need for a high repair显示文摘 | Mercado MA Chan C Orozco H | 2003 | Surg Endosc2003，17，9： | 1 |
| 15 | Preparation, Thermal Properties and Flame Retardancy of Phosphorus and Silicon-containing Epoxy Resins显示文摘 | Sponton M Mercado L A Ronda J C | 2008 | Polymer Degradation and Stability2008，93，11： | 1 |
| 16 | Surgical treatment of non-cirrhotic presinusoidal portal hyperteusion显示文摘 | Mercado MA Orozeo H Chan C | 2004 | Hepatngastroenterolngy2004，51，60： | 1 |
| 17 | Is the isolated pentagon rule merely a suggestion for endohedral fullerenes? The structure of a second egg-shaped endohedral fullerene-Gd3 N @ C, ( 39663)-C8z 显示文摘 | Mercado B Q Beavers C M 01instead M M | 2008 | J Am Chem Soc2008，130，25： | 1 |
| 18 | Preparation, thermal properties and flame retardancy of phosphorus - and silicon -containing epoxy resins 显示文摘 | SPONTON M MERCADO L A RONDA J C | 2008 | Polymer Degradation and Stability2008，93，11： | 1 |
| 19 | Prepara- tion, Thermal Properties and Flame Retardancy of Phos- phorus- and Silicon-containing Epoxy Resins显示文摘 | Sponton M Mercado L A Ronda J C | 2008 | Polymer Degradation and Stability2008，93，11： | 1 |
| 20 | N-acetylcysteine and con- trast-induced nephropathy:A meta-analysis of 13 randomized trials 显示文摘 | Zagler A Azadpour M Mercado C | 2006 | Am Heart J2006，15，1： | 1 |
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