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| 1 | Association between Helicobacterpylori, Epstein-Barr virus, human papillomavirus and gastric adenocarcinomas显示文摘AIM To correlate Helicobacter pylori(H. pylori), Epstein-Barr virus(EBV) and human papillomavirus(HPV) with gastric cancer(GC) cases in Pará State, Brazil.METHODS Tissue samples were obtained from 302 gastric adenocarcinomas. A rapid urease test was used to detect the presence of H. pylori, and the presence of the cagA gene in the HP-positive samples was confirmed by PCR. An RNA in situ hybridization test designed to complement Eber1 RNA was used to detect the presence of EBV in the samples, and the L1 region of HPV was detected using nested PCR. Positive HPV samples were genotyped and analyzed for E6 and E7 viral gene expression. Infections were also correlated with the clinical and pathological characteristics of the patients.RESULTS The majority of the 302 samples analyzed were obtained from men(65%) aged 55 years or older(67%) and were classified as the intestinal subtype(55%). All three pathogens were found in the samples analyzed in the present study(H. pylori : 87%, EBV: 20%, HPV: 3%). Overall, 78% of the H. pylori-positive(H. pylori+) samples were cag A +(H. pylori-cag A+), and there was an association between the cytotoxic product of this gene and EBV. Coinfections of H. pylori-cagA+ and EBV were correlated with the most advanced tumor stages. Although only 20% of the tumors were positive for EBV, infection with this virus was associated with distant metastasis. Only the HPV 16 and 18 strains were found in the samples, although no expression of the E6 and E7 oncoproteins was detected. The fundus of the stomach was the region least affected by the pathogens.CONCLUSION HPV was not involved in gastric tumorigenesis. Prophylactic and therapeutic measures against H. pylori and EBV may prevent the development of GC, especially the more aggressive forms. | Carolina Rosal Teixeira de Souza Marcelli Carolini Alves Almeida Andre Salim Khayat Emerson Lucena da Silva Paulo Cardoso Soares Luiz Claudio Chaves Rommel Mario Rodriguez Burbano | 2018 | World Journal of Gastroenterology2018，24，43： | 6 |
| 2 | Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development显示文摘The Dof(DNA-binding with one finger)protein family spans a group of plant transcription factors involved in the regulation of several functions,such as plant responses to stress,hormones and light,phytochrome signaling and seed germination.Here we describe the Dof-like gene family in grapevine(Vitis vinifera L.),which consists of 25 genes coding for Dof.An extensive in silico characterization of the VviDofL gene family was performed.Additionally,the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray(cv Corvina)and real-time PCR(cv Pinot Noir)analyses.The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis,tomato,poplar and already described Dof genes in other species allowed us to identify several duplicated genes.The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles.Furthermore,distinct expression patterns were identified between samples analyzed,corroborating such hypothesis.Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower,berry and seed development,highlighting their importance for grapevine growth and production.The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir. | Danielle Costenaro da Silva Vítor da Silveira Falavigna Marianna Fasoli Vanessa Buffon Diogo Denardi Porto Georgios Joannis Pappas Jr Mario Pezzotti Giancarlo Pasquali Luís Fernando Revers | 2016 | Horticulture Research2016，3，1： | 5 |
| 3 | Evidence of endometrial amino acid metabolism and transport modulation by peri-ovulatory endocrine profiles driving uterine receptivity显示文摘Background: In beef cattle, changes in the periovulatory endocrine milieu are associated with fertility and conceptus growth. A large preovulatory follicle(POF) and the resulting elevated concentrations of progesterone(P4) during diestrus positively affect pregnancy rates. Amino acids(AA) are important components of maternally derived secretions that are crucial for embryonic survival before implantation. The hypothesis is that the size of the POF and the concentration of P4 in early diestrus modulate the endometrial abundance of SLC transcripts related to AA transport and metabolism and subsequently impact luminal concentrations of AA. The follicle growth of Nelore cows was manipulated to produce two experimental groups: large POF and CL(LF-LCL group) and small POF and CL(SF-SCL group). On Day 4(D4; Experiment 1) and Day 7(D7; Experiment 2) after Gn RH-induced ovulation(Gn RH treatment = D0), the animals were slaughtered and uterine tissues and uterine washings were collected. q RT-PCR was used to evaluate the expression levels of AA transporters in D4 and D7 endometrial tissues.The concentrations of AA were quantified in D4 and D7 uterine washings by HPLC.Results: Transcript results show that, on D4, SLC6 A6, SLC7 A4, SLC17 A5, SLC38 A1, SLC38 A7 and SCLY and on D7 SLC1 A4, SLC6 A1, SLC6 A14, SLC7 A4, SLC7 A7, SLC7 A8, SLC17 A5, SLC38 A1, SLC38 A7, SLC43 A2 and DDO were more abundant in the endometria of cows from the LF-LCL group(P < 0.05). In addition, concentrations of AA in the uterine lumen were influenced by the endocrine profiles of the mother. In this context, D4 uterine washings revealed that greater concentrations of taurine, alanine and α-aminobutyric acid were present in SF-SCL(P < 0.05).In contrast, lower concentrations of valine and cystathionine were quantified on D7 uterine washings from SF-SCL cows(P < 0.05).Conclusion: The present study revealed an association between the abundance of transcripts related to AA transport and metabolism in the endometrium and specific periovulatory endocrine profiles related to the receptive status of the mother. Such insights suggest that AAs are involved in uterine function to support embryo development. | Moana Rodrigues Franca Maressa Izabel Santos da Silva Guilherme Pugliesi Veerle Van Hoeck Mario Binelli | 2017 | Journal of Animal Science and Biotechnology2017，8，4： | 4 |
| 4 | The Preoperative Manometric Pattern Predicts the Outcome of Surgical Treatment for Esophageal Achalasia显示文摘 | Renato Salvador Mario Costantini Giovanni Zaninotto Tiziana Morbin Christian Rizzetto Lisa Zanatta Martina Ceolin Elena Finotti Loredana Nicoletti Gianfranco Da Dalt Francesco Cavallin Ermanno Ancona | 2010 | Journal of Gastrointestinal Surgery2010，，11： | 2 |
| 5 | Calcium potentiates the effect of estradiol on PGF2α production in the bovine endometrium显示文摘Background: Estradiol(E2) is required for luteolysis in cows and its injection stimulates prostaglandin F2α(PGF2α)release. The main goal of our study was to investigate the ability of endometrial explants and cells treated with E2 and the calcium ionophore(CI) A23187 to synthesize PGF2α.Results: Treatment with E2 in vivo resulted in a 48.4% increase of PGF2α production by endometrial explants treated in vitro with A23187. Production of PGF2α was better stimulated with A23187 at concentrations of 10-6and10-5mol/L compared with other concentrations used. The concentration of PGF2α for untreated bovine endometrial cell cultures was 33.1 pg/m L, while for cultures treated with E2, A23187, or a combination of E2 and A23187, the PGF2α concentration was 32.5, 92.4 and 145.6 pg/m L, respectively.Conclusions: Treatment with A23187 tended to stimulate PGF2α production. In the presence of E2, A23187 significantly stimulated PGF2α synthesis. It appears that A23187 potentiates the effects of E2 with respect to synthesis of endometrial PGF2α in cattle. | Claudia Maria Bertan Membrive Pauline Martins da Cunha Flávio Vieira Meirelles Mario Binelli | 2015 | Journal of Animal Science and Biotechnology2015，6，2： | 2 |
| 6 | In vitro activities of antifungal agents alone and in combination against fluconazole-susceptible and-resistant strains of Candida dubliniensis显示文摘 | Scheid LA Mario DA Kubica TF | 2012 | Braz J Infect Dis2012，16，1： | 1 |
| 7 | Novel design methodology and comparative analysis for ZVT PWM converters with resonant auxiliary circuit 显示文摘 | Mario Lucio da Silva Martins Jumar Luis Russi Helio Leaes Hey | 2006 | IEEE Transactions on Industry Applications2006，42，3： | 1 |
| 8 | Use of thrombectomy devices in primary percutaneous coronary intervention: a systematic re- view and meta-analysis 显示文摘 | Costopoulos C Gorog DA Di Mario C | 2013 | Int J Cardiol2013，163，3： | 1 |
| 9 | Investigation of surface properties of Ru-based oxide electrodes containing Ti, Ce and Nb 显示文摘 | Mario H P Santana Leonardo M Da Silva Luiz A De Faria | 2003 | Electrochimica Acta2003，48，： | 1 |
| 10 | Use of thrombectomy devices in primary percutaneous coronary intervention: A systematic review and meta-analysis显示文摘 | Costopoulos C Gorog DA Di Mario C | 2013 | Int J Cardiol2013，163，3： | 1 |
| 11 | Magnification chromoendoscopy for the diagnosis of gastric intestinal metaplasia and dysplasia显示文摘 | Mario DR Altamiro da CP Carlos L | 2003 | Gastrointest Endosc2003，57，4： | 1 |
| 12 | Improving detection of celiac disease patients: a prospective study in iron-deficient blood donors without anemia in north Italy显示文摘 | Renato Cannizzaro Alessandro Da Ponte Maria Tabuso Mario Mazzucato Valli De Re Laura Caggiari Mara Fornasarig Stefania Maiero Enrico Orzes Vincenzo Canzonieri | 2014 | European Journal of Gastroenterology & Hepatology2014，，7： | 1 |
| 13 | Magnification chromoendoscopy for the diagnosis of gastric intestinal metaplasia and dysplasia 显示文摘 | Mario DR Altamiro da CP Carlos L | 2003 | Gastrointest Endosc2003，57，4： | 1 |
| 14 | 短跑运动员血浆中脑源性神经营养因子水平较普通人群显著升高(英文)显示文摘目的目前研究发现锻炼能增强健康人血液中脑源性神经营养因子(BDNF)的水平。BDNF水平的改变常发现于耐力运动而非力量锻炼。本研究旨在探讨厌氧型活动(如短跑)是否能改变人血浆中BDNF的浓度。方法对22名巴西100米短跑运动员,包括14名过去4年间参加过国际级奥林匹克及户外世锦赛的运动员(国际级)和8名只参加过本国比赛的运动员(国内级),以及15名此前从未参加过任何运动比赛的健康对照人群,用ELISA法检测其血浆中BDNF的水平。结果与对照组相比,国际级和国内级短跑运动员血浆中BDNF的水平均显著升高。此外,国际级短跑运动员血浆中BDNF的水平显著高于国内级运动员。结论血浆中BDNF水平的升高可能与运动加强有关。 | Paulo Roberto Correia Fulvio Alexandre Scorza Sérgio Gomes da Silva Aline Pansani Michelle Toscano-Silva Antonio Carlos de Almeida Ricardo Mario Arida | 2011 | Neuroscience Bulletin2011，27，5： | 1 |
| 15 | Effect of the Cymbopogon citratus , Maytenus ilicifolia and Baccharis genistelloides extracts against the stannous chloride oxidative damage in Escherichia coli显示文摘 | Suzana de F. Melo Scheila F Soares Rogério F da Costa Claudia R da Silva Marcia B.N de Oliveira Roberto J.A.C Bezerra Adriano Caldeira-de-Araújo Mario Bernardo-Filho | 2001 | MutRes-Genetic Toxicology and Environmental Mutagenesis2001，，1： | 1 |
| 16 | Use of thrombectomy devices in primary percutaneous coronary intervention:a systematic review and meta-analysis显示文摘 | Costopoulos C Gorog DA Di Mario C | 2013 | Int J Cardiol2013，163，： | 1 |
| 17 | An Evolutionary Approach to Technological Innovation in Agriculture- some preliminary remarks 显示文摘 | Mario Luiz Possas Sergio Salles - Filho Jos Maria da Silveira | 1996 | Research Policy1996，25，6： | 1 |
| 18 | History and Development of Breast Implants显示文摘 | Mario Rictjcns Mareo Aur6 lio da Costa Vieira Ci cero Urban | 2013 | Oncoplast Rconstr Breast Surg2013，，： | 1 |
| 19 | Human Serotonin Transporter Expression During Megakaryocytic Differentiation of MEG-01 Cells显示文摘 | Gino Giannaccini Laura Betti Lionella Palego Lara Schmid Laura Fabbrini Caterina Pelosini Claudia Gargini Ylenia Da Valle Mario Lanza Alessandro Marsili Margherita Maffei Ferruccio Santini Paolo Vitti Aldo Pinchera Antonio Lucacchini | 2010 | Neurochemical Research2010，，4： | 1 |
| 20 | Diagnosis of Helicobacter pylori Infection by Means of Reduced-Dose 13C-Urea Breath Test and Early Sampling of Exhaled Breath显示文摘 | Sylmara L.M. Pacheco Silvio K. Ogata Rodrigo S. Machado Francy Reis da Silva Patrício Mario L.E. Pardo Elisabete Kawakami | 2013 | Journal of Pediatric Gastroenterology and Nutrition2013，，5： | 1 |
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