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| 1 | Role of quorum sensing in bacterial infections显示文摘Quorum sensing(QS) is cell communication that is widely used by bacterial pathogens to coordinate the expression of several collective traits, including the production of multiple virulence factors, biofilm formation, and swarming motility once a population threshold is reached. Several lines of evidence indicate that QS enhances virulence of bacterial pathogens in animal models as well as in human infections; however, its relative importance for bacterial pathogenesis is still incomplete. In this review, we discuss the present evidence from in vitro and in vivo experiments in animal models, as well as from clinical studies, that link QS systems with human infections. We focus on two major QS bacterial models, the opportunistic Gram negative bacteria Pseudomonas aeruginosa and the Gram positive Staphylococcus aureus, which are also two of the main agents responsible of nosocomial and wound infections. In addition, QS communication systems in other bacterial, eukaryotic pathogens, and even immune and cancer cells are also reviewed, and finally, the new approaches proposed to combat bacterial infections by the attenuation of their QS communication systems and virulence are also discussed. | Israel Castillo-Juárez Toshinari Maeda Edna Ayerim Mandujano-Tinoco María Tomás Berenice Pérez-Eretza Silvia Julieta García-Contreras Thomas K Wood Rodolfo García-Contreras | 2015 | World Journal of Clinical Cases2015，3，7： | 15 |
| 2 | Congener profile assessment of polychlorinated biphenyls in dol- and tuna collected from Italian coastal waters显示文摘 | CORSOLINI and toxicity phins sharks Mar Environ S FOARDI S KANNAN K | 1995 | Res1995，40，1： | 1 |
| 3 | Time-domain parameter estimation algorithm for structures Ⅱ: Nemerical simulation studies 显示文摘 | Banan MaR Hjelmstad K D | 1995 | Eng Mech1995，121，3： | 1 |
| 4 | A multi-ob-jective model for locating fire stations 显示文摘 | MASOOD A B MAR K M COLONEL A A | 1998 | European Jour-nal of Operational Research1998，110，： | 1 |
| 5 | A comparison between adipose tissue and dental pulp as sources of MSCs for tooth regeneration显示文摘 | Hung CN Mar K Chang HC | 2011 | Biomaterials2011，32，29： | 1 |
| 6 | Synchronous recruitment of epigenetic modifiem to endotoxin synergistically activated Tnf-a gene in acute kidney injury显示文摘 | Bomsztyk K Flanagin S Mar D | 2013 | PLoS One2013，8，7： | 1 |
| 7 | Fish Classification Based on Robust Features Extraction From Color Signature Using BackPropagation Classifier显示文摘 | Alsmadi Mutasem K mar Khairuddin B Noah Shahrul A.M | | 0，，： | 1 |
| 8 | Expression of matrix metalloproteinases in benign and malignant follicular thyroid lesions显示文摘 | Cho Mar K Eimoto T Tateyama H | 2006 | Histopathology2006，48，3： | 1 |
| 9 | Impaired vascular growth in late adolescence after intrauterine growth restriction显示文摘 | Brodszki J Lnne T Marsál K | 2011 | Circulation2011，111，20： | 1 |
| 10 | Genetic,biochemical,and molecular characterization of the polypeptide transportassociated domain of Escherichia coli BamA显示文摘 | Workman P Heide K Giuliano N Lee N Mar J Vuong P Bennion D Misra R | | 0，，13： | 1 |
| 11 | Mapping mine wastes and analyzingareas affected by selenium-rich water runoff in southeastIdaho using AVIRIS imagery and digital elevation data 显示文摘 | Mars J C Crowley J K | 2003 | Remote Sensing of Environment2003，84，： | 1 |
| 12 | Isolation, chemical investigation and antiviral activity of polysaccharides from Gracilaria corticata (Gracilariaceae, Rhodophyta)显示文摘 | Sutapa Mazumder Prodyut K Ghosal Carlos A Pujol Mar??a J Carlucci Elsa B Damonte Bimalendu Ray | 2002 | International Journal of Biological Macromolecules2002，，1： | 1 |
| 13 | Mapping mine wastes and analyzing areas affected by elenium - rich water runoff in southeast I- daho using AVIRIS imagery and digital elevation data 显示文摘 | John C Mars James K Crowley | 2003 | Remote Sensing of Environment2003，，84： | 1 |
| 14 | A novel class of inhibitors for steroid 5α-reductase:Synthesis and evaluation of umbelliferone derivatives显示文摘 | Fan G J Mar W Park M K | 2001 | Bioorg Med Chem Lett2001，11，17： | 1 |
| 15 | Expression of matrix metalloproteinases in benign and malignant follicular thyroid lesions显示文摘 | Cho Mar K Eimoto T Tateyama H | 2006 | Histopathology2006，48，3： | 1 |
| 16 | A nodified extraction of DNA from paraffin-embedded tissue samples used in identity testing 显示文摘 | Chiriboga E Mar K | 1999 | Can Soc Forens Sci J1999，32，： | 1 |
| 17 | Localization of mRNAs encoding acetylcholinesterase and butyrylcholinesterase in the rat spinal cord by nonradioactive in situ hybridization显示文摘 | Mis K Mars T Jevsek M | 2003 | J Histochem Cytochem2003，51，： | 1 |
| 18 | Recurrent thrombosis prevention with intravenous immunogIobulin and hydroxychloroquine dur- ing pregnancy in a patient with history of catastrophic antiphos- pholipid syndrome and pregnancy loss 显示文摘 | Mar N Kosowicz R Hook K | 2014 | J Thromb Thromboly- sis2014，38，： | 1 |
| 19 | Fabrication of tapered conicalshaped titania nanotubes 显示文摘 | Mar G K Varghese 0 K Paulose M | 2003 | Materials Research2003，18，11： | 1 |
| 20 | HP0953-hypothetical virulence factor overexpresion and localization during Helicobacter pylori infection of gastric epithelium显示文摘BACKGROUND The high prevalence and persistence of Helicobacter pylori(H. pylori) infection, as well as the diversity of pathologies related to it, suggest that the virulence factors used by this microorganism are varied. Moreover, as its proteome contains 340hypothetical proteins, it is important to investigate them to completely understand the mechanisms of its virulence and survival. We have previously reported that the hypothetical protein HP0953 is overexpressed during the first hours of adhesion to inert surfaces, under stress conditions, suggesting its role in the environmental survival of this bacterium and perhaps as a virulence factor.AIM To investigate the expression and localization of HP0953 during adhesion to an inert surface and against gastric(AGS) cells.METHODS Expression analysis was performed for HP0953 during H. pylori adhesion. HP0953 expression at 0,3, 12, 24, and 48 h was evaluated and compared using the Kruskal-Wallis equality-of-populations rank test. Recombinant protein was produced and used to obtain polyclonal antibodies for immunolocalization. Immunogold technique was performed on bacterial sections during adherence to inert surfaces and AGS cells, which was analyzed by transmission electron microscopy. HP0953 protein sequence was analyzed to predict the presence of a signal peptide and transmembrane helices, both provided by the ExPASy platform, and using the GLYCOPP platform for glycosylation sites. Different programs, via, I-TASSER, RaptorX, and HHalign-Kbest, were used to perform three-dimensional modeling.RESULTS HP0953 exhibited its maximum expression at 12 h of infection in gastric epithelium cells.Immunogold technique revealed HP0953 localization in the cytoplasm and accumulation in some peripheral areas of the bacterial body, with greater expression when it is close to AGS cells.Bioinformatics analysis revealed the presence of a signal peptide that interacts with the transmembrane region and then allows the release of the protein to the external environment. The programs also showed a similarity with the Tip-alpha protein of H. pylori. Tip-alpha is an exotoxin that penetrates cells and induces tumor necrosis factor alpha production, and HP0953 could have a similar function as posttranslational modification sites were found;modifications in turn require enzymes located in eukaryotic cells. Thus, to be functional, HP0953 may necessarily need to be translocated inside the cell where it can trigger different mechanisms producing cellular damage.CONCLUSION The location of HP0953 around infected cells, the probable posttranslational modifications, and its similarity to an exotoxin suggest that this protein is a virulence factor. | Nancy K Arteaga-Resendiz Gerardo E Rodea Rosa María Ribas-Aparicio Alma L Olivares-Cervantes Juan Arturo Castelán-Vega Joséde Jesús Olivares-Trejo Sandra Mendoza-Elizalde Edgar O López-Villegas Christian Colín Pamela Aguilar-Rodea Alfonso Reyes-López Marcela Salazar García Norma Velázquez-Guadarrama | 2022 | World Journal of Gastroenterology2022，28，29： | 1 |
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