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1Injectable hydrogels for cartilage and bone tissue engineering显示文摘Tissue engineering has become a promising strategy for repairing damaged cartilage and bone tissue. Among the scaffolds for tissue-engineering applications, injectable hydrogels have demonstrated great potential for use as three-dimensional cell culture scaffolds in cartilage and bone tissue engineering, owing to their high water content, similarity to the natural extracellular matrix(ECM), porous framework for cell transplantation and proliferation, minimal invasive properties, and ability to match irregular defects. In this review, we describe the selection of appropriate biomaterials and fabrication methods to prepare novel injectable hydrogels for cartilage and bone tissue engineering. In addition, the biology of cartilage and the bony ECM is also summarized. Finally, future perspectives for injectable hydrogels in cartilage and bone tissue engineering are discussed.Mei Liu Xin Zeng Chao Ma Huan Yi Zeeshan Ali Xianbo Mou Song Li Yan Deng Nongyue He 2017Bone Research2017,5,2:54
2Intraoperative endoscopic sphincterotomy for common bile duct stones during laparoscopic cholecystectomy显示文摘INTRODUCTION The advent of laparoscopic cholecystectomy(LC)inthe late 1980s gained widespread acceptance withina short period of time and has become the preferredtreatment for symptomatic gallstones,but themanagement of coexisting gallbladder and commonbile duct(CBD)stones has remained controversialbecause the various strategies proposed have theirlimitations.In fact,choledocholithiasisDe Fei Hong Ming Gao Urs Bryner Xiu Jun Cai Yi Ping Mou 2000World Journal of Gastroenterology2000,6,3:19
3Drug-resistant gene based genotyping for Acinetobacter baumannii in tracing epidemiological events and for clinical treatment within nosocomial settings显示文摘背景 Acinetobacter baumannfi 在全世界作为与严重感染和医院的爆发有关的重要病原体出现了。经常使用的方法,然而,脉搏地胶化电气泳动(PFGE ) 和在 Acinetobacter baumannii genotyping 的放大碎片长度多型性(AFLP ) 缺乏药抵抗的直接分子的证明。这研究被进行基于药建立一个打字方法与在从2005年1月的医院的流行病或到2005年10月的 outbreak.Methods 的经期的传统的 PFGE 和 AFLP 相对照的抵抗基因鉴定,来自特别护理单位的 Acinetobacter 种类的 27 紧张,在 Ningbo 的第二所隶属于的医院被孤立,包括流行、分散的事件。危险性测试, PFGE, AFLP 和打字的药抵抗基因(DRGT ) 在外面是 carded 证实药抵抗并且分别地分析 genotyping。PFGE 被用作一本参考书评估 Acinetobacter 的 27 紧张显示了的 DRGT 和 AFLP.Results 的 typeability 多重抗菌素抵抗和药抵抗基因,和 -lactamase 基因在 85.2% 紧张被检测。尽管小差别存在, DRGT 的结果比得上在有不同的药抵抗的 Acinetobacter 紧张的 PFGE,并且甚至建议了不同药抵抗的紧张的一堂分子的进化功课。AFLP 显示出在紧张之间的大多型性并且在区分比作 AFLP 和 PFGE 的药 resistance.Conclusion 有弱能力, DRGT 是有用的分析医院的感染和爆发的局部性的分子的传染病学,它将有益于临床的诊断和治疗。JIN Hui XU Xiao-min MI Zu-huang MOU Yi LIU Pei 2009Chinese Medical Journal2009,,3:18
4A Study of the Technique of Western Blot for Diagnosis of Lyme Disease caused by Borrelia afzelii in China显示文摘Objective To study the technique of Western blot for the diagnosis of Lyme disease caused by Borrelia afzelii in China and to establish the standard criteria by operational procedure. Methods FP1, which is the representative strain of B. afzelii in China, was analyzed by SDS‐PAGE, electro transfer and immunoblotting assays. The molecular weights of the protein bands of FP1 were analyzed by Gel‐Pro analysis software. In a study using 451 serum samples (159 patients with Lyme disease and 292 controls), all observed bands were recorded. The accuracy of the WB as a diagnostic test was established by using the ROC curve and Youden index. Results Criteria for a positive diagnosis of Lyme disease were established as at least one band of P83/100, P58, P39, OspB, OspA, P30, P28, OspC, P17, and P14 in the IgG test and at least one band of P83/100, P58, P39, OspA, P30, P28, OspC, P17, and P41 in the IgM test. For IgG criteria, the sensitivity, specificity and Youden index were 69.8%, 98.3%, and 0.681, respectively; for IgM criteria, the sensitivity, specificity and Youden index were 47%, 94.2%, and 0.412, respectively. Conclusion Establishment of WB criteria for B. afzelii is important in validating the diagnostic assays for Lyme disease in China.LIU Zhi Yun HAO Qin HOU Xue Xia JIANG Yi GENG Zhen WU Yi Mou WAN Kang Lin 2013Biomedical and Environmental Sciences2013,26,3:7
5Generation of tryptophan hydroxylase 2 gene knockout pigs by CRISPR/Cas9-mediated gene targeting显示文摘Unbalanced brain serotonin(5-HT) levels have implications in various behavioral abnormalities and neuropsychiatric disorders. The biosynthesis of neuronal 5-HT is regulated by the rate-limiting enzyme, tryptophan hydroxylase-2(TPH2). In the present study, the clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated(Cas) system was used to target the Tph2 gene in Bama mini pig fetal fibroblasts. It was found that CRISPR/Cas9 targeting efficiency could be as high as 61.5%, and the biallelic mutation efficiency reached at38.5%. The biallelic modified colonies were used as donors for somatic cell nuclear transfer(SCNT) and 10 Tph2 targeted piglets were successfully generated. These Tph2 KO piglets were viable and appeared normal at the birth.However, their central 5-HT levels were dramatically reduced, and their survival and growth rates were impaired before weaning. These Tph2 KO pigs are valuable large-animal models for studies of 5-HT deficiency induced behavior abnomality.Ze Li Hai-Yuan Yang Ying Wang Man-Ling Zhang Xiao-Rui Liu Qiang Xiong Li-Ning Zhang Yong Jin Li-Sha Mou Yan Liu Rong-Feng Li Yi Rao Yi-Fan Dai 2017The Journal of Biomedical Research2017,31,5:4
6MC simulation of a PGNAA system for on-line cement analysis显示文摘A prompt gamma neutron activation analysis system with a 252Cf neutron source for on-line cement analysis has been simulated with the MCNP code.The results indicate that the optimum arrangement is a Bi shield of 20-mm thickness,a polyethylene moderator of 50-mm thickness,a source-to-sample distance of 70 mm,and cement samples of 1200 mm×600 mm×170 mm.To absorb thermal neutrons and suppress low-energy γ-rays,the optimum-sized sheets are 150 mm×7 mm Cd,and 150 mm×15 mm Pb.YANG Jianbo TUO Xianguo LI Zhe MU Keliang CHENG Yi MOU Yunfeng 2010Nuclear Science and Techniques2010,21,4:4
7Lipid-associated membrane proteins of Mycoplasma penetrans induce production of proinflammatory cytokines in human monocytic cells显示文摘The aim of this study is to explore potential pathogenicity of Mycoplasma penetrans, and to investigate whether M.penetrans lipid-associated membrane proteins (LAMPs) could induce human monocytic cell line (THP-1) to produce some proinflammatory cytokines in vitro, including interleukin-1β(IL-1β) , tumor necrosis factor alpha (TNF-α) , and IL-8. THP-1 was stimulated with different concentrations of M .penetrans LAMPs and at different time to analyze the production of human IL-1β, TNF-αand IL-8. The protein levels of human IL-1β, TNF-αand IL-8 were measured by enzyme-linked immunoadsorbent assay (ELISA) and the mRNA levels of these proinflamrnatory cytokines were detected by reverse transcriptase -PCR (RT-PCR). It was demonstrated in the present study that the production of IL-1β, TNF-αand IL-8 increased in dose- and time-dependent manner after stimulation with M. penetrans LAMPs in THP-1 cells. M.penetrans LAMPs also induced the expression of IL-1β, TNF-αand IL-8 mRNA. The production of IL-1β, TNF-αand IL-8 and the expression of mRNA were down-regulated by pyrrolidine dithiocarbamate (PDTC). This study demonstrated that M. penetrans LAMPs can induce the production of proinflammatory cytokines in human monocytic cells in vitro, thus suggesting that it may be an important etiological factor.YI MOU WU MIN JUN YU LI ZHI TAN XIAO XING YOU 2006Journal of Microbiology and Immunology2006,4,4:3
8Cystic meconium peritonitis with jejunoileal atresia:Is it associated with unfavorable outcome?显示文摘AIM To compare the outcome between patients with jejunoileal atresia(JIA) associated with cystic meconium peritonitis(CMP) and patients with isolated JIA(JIA without CMP).METHODS A retrospective study was conducted for all neonates with JIA operated in our institute from January 2005 to January 2016.Demographics including the gestation age,sex,birth weight,age at operation,the presence of associated syndrome was recorded.Clinical outcome including the type of operation performed,operative time,the need for reoperation and mortality were studied.The demographics and the outcome between the 2 groups were compared.RESULTS During the study period,53 neonates had JIA underwent operation in our institute.Seventeen neonates(32%) were associated with CMP.There was no statistical difference on the demographics in the two groups.Patients with CMP had earlier operation than patients with isolated JIA(mean 1.4 d vs 3 d,P = 0.038).Primaryanastomosis was performed in 16 patients(94%) with CMP and 30 patients(83%) with isolated JIA(P = 0.269).Patients with CMP had longer operation(mean 190 min vs 154 min,P = 0.004).There were no statistical difference the need for reoperation(3 vs 6,P = 0.606) and mortality(2 vs 1,P = 0.269) between the two groups.CONCLUSION Primary intestinal anastomosis can be performed in94% of patients with JIA associated with CMP.Although patients with CMP had longer operative time,the mortality and reoperation rates were low and were comparable to patients with isolated JIA.Kin Wai Edwin Chan Kim Hung Lee Hei Yi Vicky Wong Siu Yan Bess Tsui Yuen Shan Wong Kit Yi Kristine Pang Jennifer Wai Cheung Mou Yuk Him Tam 2017World Journal of Clinical Pediatrics2017,6,1:3
9Focal nodular hyperplasia of the liver: pathological analysis of 11 cases显示文摘BACKGROUND: Focal nodular hyperplasia (FNH) is a benign tumor-like lesion of the liver, predominantly affect- ing women. Its etiology is obscure and its pathogenesis is poorly understood. FNH should be differentiated from oth- er benign and malignant hepatic lesions. The aim of this study was to explore the pathological characteristics of FNH of the liver. METHODS: Eleven patients with FNH were studied retro- spectively by using hematoxylin and eosin, immunohisto- chemical and histochemical staining. RESULTS: In 8 female and 3 male FNH patients aged 19 to 54 years (mean 32), most of lesions showed central scars macroscopically. Microscopically 8 patients were found of classical type, 2 were of telangiectic type, and 1 was of mixed type. CONCLUSION: FNH is an uncommon benign hyperplastic lesion of the liver. It should be differentiated from hepato- cellular adenoma, alpha-fetoprotein negative hepatocellular carcinoma, and fibrolamellar carcinoma.Yi Pan, Zhao-Ming Wang, Ling-Jun Mou, Xiao-Dong Teng, Zhou-Jun Zheng and Li-Xiong Ying Hangzhou, ChinaDepartments of Pathology and General Surgery First Affilia- ted Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China 2004Hepatobiliary & Pancreatic Diseases International2004,3,2:3
10Laparoscopic excision of Meckel's diverticulum in children:What is the current evidence?显示文摘Complications aroused from Meckel's diverticulum tend to developed in children.Children presented with abdominal pain,intestinal obstruction,intussusception or gastrointestinal bleeding may actually suffered from complicated Meckel's diverticulum.With the advancement of minimally invasive surgery(MIS) in children,the use of laparoscopy in the diagnosis and subsequent laparoscopic excision of Meckel's diverticulum has gained popularity.Recently,single incision laparoscopic surgery(SILS) has emerged as a new technique in minimally invasive surgery.This review offers the overview in the development of MIS in the management of children suffered from Meckel's diverticulum.The current evidence in different laparoscopic techniques,including conventional laparoscopy,SILS,the use of special laparoscopic instruments,intracorporeal diverticulectomy and extracorporeal diverticulectomy in the management of Meckel's diverticulum in children were revealed.Kin Wai Edwin Chan Kim Hung Lee Hei Yi Vicky Wong Siu Yan Bess Tsui Yuen Shan Wong Kit Yi Kristine Pang Jennifer Wai Cheung Mou Yuk Him Tam 2014World Journal of Gastroenterology2014,20,41:2
11Development and Evaluation of a MAb-Based ELISA for Detection of Chlamy- dophila pneumoniae Infection with Variable Domain 2 and 3 of the Major Outer Membrane protein显示文摘Objective This paper aims to develop a monoclonal antibodies (MAbs)- based ELISA for detecting Chlamydophila pneumoniae (C. pneumoniae) antigens in humans with the variable domains (VD) 2 and 3 of the major outer membrane protein (MOMP VD2-VD3 ) and to assess its sensitivity and specificity by comparing with a widely used MAb that is able to recognize the elementary bodies of C. pneumoniae. Methods MOMP VD2-VD3 were overexpressed in Escherichia coli and purified by affinity chromatography. Mice were immunized with the recombinant antigen, and hybridomas secreting MAbs were screened. Three stable hybridomas clones were selected and named 5D6, 7G3, and 8C9. The MAbs-based ELISA was scrutinized for species-specific recognition with a number of human throat swab samples from Group I (156 patients with typical respiratory illness clinically confirmed before) and Group II (57 healthy donors). Results In Group I, 55 positive cases were detected by anti-EB MAb-based ELISA, 51 cases were positive by MAbs 5D6-based ELISA, and 33 and 38 cases were positive by MAb 8C9 and 7G3-based ELISA respectively. Of the 57 samples from Group II 'healthy donors', 5 were positive and 52 were negative with both anti-EB and 5D6-based tests, while 2 and 3 positive cases were identified by the other two MAb-based ELISAs respectively. Conclusion The novel MOMP VD2-VD3 MAb-based assay may have higher specificity than the anti-EB MAb, which may possibly be used as an alternative tool for the diagnosis of C. pneumoniae infection.ZHOU Zhou WU Yi Mou CHEN Li Li LIU Guang Chao LIU Liang Zhuan ZHOU An Wen ZHANG Jun Hua 2012Biomedical and Environmental Sciences2012,25,6:2
12Binding pancreaticojejunostomy: 150 consecutive cases without leakage显示文摘Shu You Peng M.D. Yi Ping Mou M.D. Yin Bin Liu M.D. Ying Su M.D. Cheng Hong Peng M.D. Xiu Jun Cai M.D. Yu Lian Wu M.D. Lin Hua Zhou M.D 2003Journal of Gastrointestinal Surgery2003,,7:2
13Progression rate to primary angle closure following laser peripheral iridotomy in primary angle-closure suspects: a randomised study显示文摘AIM:To report the progression rate(PR)to primary angle closure(PAC)following laser peripheral iridotomy(LPI)in PAC suspects(PACS).METHODS:Prospective,randomized controlled interventional clinical trial conducted at the Handan Eye Hospital,China.Totally 134 bilateral PACS,defined as non-visibility of the posterior trabecular meshwork for≥180 degrees on gonioscopy were randomly assigned to undergo LPI in one eye.Gonioscopy and Goldmann applanation tonometry were performed prior to,on day 7 and 12 mo post LPI.RESULTS:Eighty of 134 patients(59.7%)could be followed up at one year.The mean intraocular pressure(IOP)in treated eyes was 15.9±2.6 mm Hg at baseline,15.4±3.0 mm Hg on day 7;16.5±2.9 mm Hg at one month,and 15.5±2.9 mm Hg at 12 mo;the IOP in untreated eyes was similar(P=0.834).One or more quadrants of the angle opened in 93.7%of the LPI treated eyes,but 67.0%(53/79)remained closed in two or more quadrants.The PR to PAC in untreated eyes was 3.75%and one developed acute angleclosure glaucoma(AACG);the PR to PAC in treated eyes was 2.5%and none had developed peripheral anterior synechia(PAS)or AACG.CONCLUSION:LPI can open some of the occludable angle in the majority of eyes with PACS,but 67%continue to have non-visibility of the trabecular meshwork for over 180 degrees.Da-Peng Mou Yuan-Bo Liang Su-Jie Fan Yi Peng Ning-Li Wang Ravi Thomas 2021International Journal of Ophthalmology(English edition)2021,14,8:2
14CaWRKY40, a WRKY protein of pepper, plays an important role in the regulation of tolerance to heat stress and resistance to Ralstonia solanacearum infection显示文摘FENG‐FENG DANG YU‐NA WANG LU YU THOMAS EULGEM YAN LAI ZHI‐QIN LIU XU WANG AI‐LIAN QIU TING‐XIU ZHANG JING LIN YAN‐SHENG CHEN DE‐YI GUAN HAN‐YANG CAI SHAO‐LIANG MOU SHUI‐LIN HE 2012Plant, Cell & Environment2012,,:2
15Eukaryotic expression of outer membrane protein Gpd from Treponema pallidum and preliminary studies on its immune response in rabbits显示文摘The Gpd gene was amplified from the genomic DNA of Treponema pallidum and cloned into the appropriate site of pcDNA3.1(+) vector. The expression of pcDNA3.1(+)-Gpd in HeLa cells was tested with Western blotting and technology of immunocytochemistry. New Zealand rabbits were immunized with the eukaryotic expression recombinant pcDNA3.1(+)-Gpd. A fusion protein of Gpd with 4.1 kDa has been effectively expressed in HeLa cells, which were detected by Western blotting and the immunocytochemistry techniques. The New Zealand rabbits were able to elicit the specific antibody after immunization with the nucleic acid vaccine. The antibody titer could reach as high as 1∶1024 after 2 weeks of the third injection; and the splenocytes proliferated evidently due to the Gpd protein stimulation. Both the antibody titer and the splenocytes proliferation were higher substantially than those of controls (P<0.01). All above data will contribute to an experimental basis of further study of the biological function of Gpd protein as well as DNA vaccine for syphilis.FEI JUN ZHAO YI Mou WU XIAO HONG ZHANG SnUANG QUAN LIU MIN JUN YU LI SHENG ZHAN 2005Journal of Microbiology and Immunology2005,3,2:2
16Synthesis and evaluation of 2-cyano-3, 12-dioxooleana-1, 9(11)-en-28-oate-13β, 28-olide as a potent anti-inflammatory agent for intervention of LPS-induced acute lung injury显示文摘The present study was designed to synthesize 2-Cyano-3, 12-dioxooleana-1, 9(11)-en-28-oate-13β, 28-olide(1), a lactone derivative of oleanolic acid(OA) and evaluate its anti-inflammatory activity. Compound 1 significantly diminished nitric oxide(NO) production and down-regulated the m RNA expression of iNOS, COX-2, IL-6, IL-1β, and TNF-α in lipopolysaccharide(LPS)-stimulated RAW264.7 cells. Further in vivo studies in murine model of LPS-induced acute lung injury(ALI) showed that 1 possessed more potent protective effects than the well-known anti-inflammatory drug dexamethasone by inhibiting myeloperoxidase(MPO) activity, reducing total cells and neutrophils, and suppressing inflammatory cytokines expression, and thus ameliorating the histopathological conditions of the injured lung tissue. In conclusion, compound 1 could be developed as a promising anti-inflammatory agent for intervention of LPS-induced ALI.MOU Yi JIAN Yan-Lin CHEN Tong HUANG Zhang-Jian QIAO Yi-Xue PENG Si-Xun ZHANG Da-Yong JI Hui ZHANG Yi-Hua 2017Chinese Journal of Natural Medicines2017,15,5:2
17Use of laparoscopy as the initial surgical approach of impalpable testes:10-year experience显示文摘AIM: To review the experience in the management of impalpable testes using laparoscopy as the initial approach and the need for inguinal exploration.METHODS: From January 2004 to June 2014, 339 patients with undescended testes underwent operation in our institute. Fifty patients(15%) had impalpable testes. All children with impalpable testes underwent initial laparoscopy. A retrospective review was conducted on this group of patients and the outcome was analyzed.RESULTS: Forty children had unilateral impalpable testis. Ten children had bilateral impalpable testes. Thirty-one children(78%) in the unilateral group underwent subsequent inguinal exploration while 4 children(40%) in the bilateral group underwent inguinal exploration(P < 0.05). Orchidopexy was performed in 16 children(40%) in the unilateral group and 9 children(90%) in the bilateral group(P < 0.05). Regarding the 24 children with unilateral impalpable testis and underwent orchidectomy for testicular nubbin(n = 19) or atrophic testes(n = 2) or has vanishing testes(n = 3); contralateral testicular hypertrophy was noticed in 10(41%). No intra-operative complication was encountered. Two children after staged Fowler-Stephensprocedure and 1 child after inguinal orchidopexy had atrophic testes.CONCLUSION: The use of laparoscopy in children with impalpable testes is a safe procedure and can guide the need for subsequent inguinal exploration. Children with unilateral impalpable testis were associated with an increased need for inguinal exploration after laparoscopy. Orchidopexies could be performed successfully in 90% of children with bilateral impalpable testes.Kin Wai Edwin Chan Kim Hung Lee Hei Yi Vicky Wong Siu Yan Bess Tsui Yuen Shan Wong Kit Yi Kristine Pang Jennifer Wai Cheung Mou Yuk Him Tam 2015World Journal of Clinical Pediatrics2015,4,4:2
18Preparation and analysis of immunocompetence of recombinant fusion protein of the immunodominant region in chlamydial protease-like activity factor from Chlamydophila pneumoniae and its application in serodiagnosis显示文摘To clone the gene coding the immunodominant region in the chlamydial protease-like activity factor(CPAF)from Chlamydophila pneumoniae,to analyze immunocompetence of the expressed protein, and to evaluate its value in serodiagnosis,the CPAF immunodominant region gene was amplified,ligated into a pGEX6p-2 vector,and then the expressed recombinant protein was purified with glutathione S- transferase(GST)agarose gel FF after renaturation,then identified by SDS-PAGE and Western blot.A new indirect ELISA was developed with the purified protein as coating antigen.The immunogenicity of the recombinant protein was evaluated by immunization to New Zealand rabbits,and its immunoreactivity was analyzed by reacting with anti-C,pneumoniae antibody.300 clinical sera samples were respectively de- tected by microimmunofluorescence(MIF)as reference method and the indirect ELISA,and the differ- ence between the two methods was analyzed.Cross-reactivity against Chlamydia trachomatis was investi- gated with the indirect ELISA to detect anti-C,trachomatis positive antisera.The results indicated that a 51.3 kDa recombinant protein was obtained.Western blot assay proved that the recombinant protein could merely specifically react with human anti-C.pneumoniae antisera.The titers of the specific IgG an- tibodies in the immunized New Zealand rabbits were above 1:16 000.Anti-C.pneumoniae IgG positive and negative reference sere were detected with the indirect ELISA,and the concordance rate of negative and positive results were both 100%(40/40).The sensitivity and specificity of the indirect ELISA in comparison with MIF were 93.8%(45/48)and 100%(252/252)separately by detecting 300 clinical sera samples,and the concordance rate between the two methods was 99.0%.No cross reaction against C.trachomatis was found with the indirect ELISA to detect anti-C,trachomatis positive antisera.In con- clusion,the prepared recombinant protein of the CPAF immunodominant region shows excellent immuno- competence and can be used to develop a new indirect ELISA as a method to detect anti-C.pneumoniae antibody for diagnosis of C.pneumoniae infection.JIANG HUA ZHENG YI MOU WU TAO DING LI LICHEN JIA QIANG LIU SHUANG QUAN LIU 2007Journal of Microbiology and Immunology2007,5,2:2
19The Efficacy of Second‐Line Anti‐ Helicobacter pylori Therapy Using an Extended 14‐Day Levofloxacin/Amoxicillin/Proton‐Pump Inhibitor Treatment – A Pilot Study显示文摘Seng‐Kee Chuah Wei‐Chen Tai Pin‐I Hsu Deng‐Chyang Wu Keng‐Liang Wu Chung‐Mou Kuo Yi‐Chun Chiu Ming‐Luen Hu Yeh‐Pin Chou Yuan‐Hung Kuo Chih‐Ming Liang King‐Wah Chiu Tsung‐Hui Hu 2012Helicobacter2012,,5:2
20Successful en bloc resection of papillary neuroendocrine tumors by duodenoscope using en- doscopic submucosal dissection method 显示文摘Yi H Wu C Mou Y 2012Clin Res Hepa- tol Gastroentero12012,36,5:1
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