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| 1 | 超声波辅助农杆菌介导CP基因转化番小瓜(Carioca papaya L.)的有效方法显示文摘本研究探索了通过农杆菌介导,超声波辅助处理,转化番木瓜胚性愈伤组织,获得转基因植株的有效方法。分别将含有日本PLDMV 外壳蛋白基因(PTi-Epj-TL-PLDMV)和含有台湾PRSV 菌株、美国夏威夷PRSV 菌株、泰国PRSV 菌株及日本PLDMV 菌株的多元外壳蛋白基因编码序列(PTi-NP-YKT)插入双元载体质粒pGA482G,借助于农杆菌系LBA4404将双元载体上的外壳蛋白基因和新霉素磷酸转移酶基因(nptⅡ)转移到番木瓜品种Sunset 的胚性愈伤组织中,从而获得抗卡那霉素的转化再生植株。试验着重在转化方法上进行探索。结果表明,农杆菌过夜培养后,用高渗透压培养液(1/2 MS+6%蔗糖+1%葡萄糖,pH 5.7)调整至光密度OD_(600(?)m)=0.15-0.20,然后用该菌液感染材料30min,其间辅以超声波处理,可以大大提高转化效率。用15ml 无菌离心管装载胚性愈伤材料进行15s 的超声波处理,在80块被转化的胚性愈伤中获得21个CP 基因G 转化系(26.3%),而在对照处理64块胚性愈伤中仅获得1个转化系(1.6%);在经过15s 的超声波处理48块被转化的胚性愈伤中获得8个CP 基因B 转化系(16.7%),而在对照处理25块胚性愈伤中未出现转化系。上述操作方法用在两种CP 基因转化上均表现出相似的效果。试验还表明:120mg/L 是卡那霉素抗性筛选的最佳浓度。抗性筛选9个月后,在421块胚性愈伤组织中产生了42个抗卡那霉素的转化系。所获得的转基因植株分别用PCR 和Southern 印迹杂交进行了鉴定。 | 姜玲 MAOKA Tetsuo KOMORI Sadao FUKAMACHI Hiroshi KATO Hidenori OGAWA Kazunori | 2004 | 实验生物学报2004，37，3： | 11 |
| 2 | Siphonaxanthin, a marine carotenoid from green algae, effectively induces apoptosis in human leukemia (HL-60) cells显示文摘 | Ponesakki Ganesan Kenji Noda Yuki Manabe Takeshi Ohkubo Yukihisa Tanaka Takashi Maoka Tatsuya Sugawara Takashi Hirata | 2011 | BBA - General Subjects2011，，： | 1 |
| 3 | 2,2’-β-Hydroxylase(CrtG)isinvolved in carotenogenesis of both nostoxanthin and 2-hydroxymyxol 2’-fucoside in Thermosynechococcus elongatus strain BP-1显示文摘 | IWAI M MAOKA T IKEUCHI M | 2008 | Plant CellPhysiol2008，49，： | 1 |
| 4 | The occurrence of Potato virus Y on potato in Syria显示文摘 | Chikh Ali M Katayama K Maoka T | 2006 | Jpn J Trop Agric2006，50，： | 1 |
| 5 | The occurrence of enantiomeric and meso-astaxanthin in aquatic animals显示文摘 | MATSUNO T MAOKA T KATSUYAMA M | | 0，，： | 1 |
| 6 | Characterization and singlet oxygen quenching activity of (3R)-3-hydroxy-4-ketotorulene and (3R)-3-hydroxy-4-keto-),-carotene from the yeast Xanthophyllomyces dendrorhrous显示文摘 | MAOKA T ETOH T OSAWA A | 2012 | Journal of Oleo Science2012，61，7： | 1 |
| 7 | Stereochemical investigation of carotenoids in the Antarctic krill Euphausia superb显示文摘 | MAOKA T KATSUYAMA M KANEKO N | 1985 | Bulletin of the Japanese Society of Scientific Fisheries1985，51，10： | 1 |
| 8 | Carotenoids and their metabolism in the goldfish Carassius auratus(Hibuna)显示文摘 | Ohkubo M Tsushima M Maoka T | 1999 | Comp Biochem Physiol1999，，124： | 1 |
| 9 | Carotenoids in the Japanese stick insect Neophirasea japonica显示文摘 | MATSUNO T MAOKA T TORIIMINAMI Y | | 0，，： | 1 |
| 10 | Stereochemical investigation of carotenoids in the antarctic krill Euphausia superba显示文摘 | MAOKA T KATSUYAMA M KANEKO N | | 0，，： | 1 |
| 11 | A new approach to chemoe -mbolization therapy for hepatoma using ethiodized oil,cisplation and gelatin sponge显示文摘 | Sasaki Y Maoka Kasugai H | 1987 | Cancer1987，60，5： | 1 |
| 12 | Novel diterpenes, excoeearins M and N from the resinous wood of Excoecaria agallocha 显示文摘 | Konishi T Konoshima T Maoka T | 2000 | Tetrahedron Lett2000，41，34： | 1 |
| 13 | The complete nucleotide sequence and biotype variability of Papaya leaf distortion mosaic virus显示文摘 | Maoka T Hataya T | 2005 | Phytopathology2005，95，2： | 1 |
| 14 | 3-β-Glucosyl-3'-β-Quinovosyl Zeaxanthin, a Novel Carotenoid Glycoside Synthe-sized by Escherichia Coli Ceils Expressing the Pantoea Ananatis Carotenoid Biosynthesis Gene Cluster显示文摘 | Choi S K Osawa A Maoka T | 2013 | Applied M crobiology and Biotechnology2013，97，19： | 1 |
| 15 | A point mutation changes the serotype of a potato virus Y isolate; genomic determination of the serotype of PVY strains 显示文摘 | Chikh A M Maoka T Natsuaki K T | 2007 | Virus Genes2007，35，2： | 1 |
| 16 | Major Carotenoid Isolated from Paracoccus Schoinia NBRC 1006377 is Adonix anthin Diglucoside显示文摘 | Takaichi S Maoka T Akimoto N | 2006 | Journal of natural products2006，69，12： | 1 |
| 17 | Dihydrobenzofuran lignans from Boreava orientalis 显示文摘 | Sakushima A Coskun M Maoka T etal | 1996 | Phytochemistry1996，43，6： | 1 |
| 18 | Carotenids with a 5,6-dihydro-5,6-dihydroxy-β-end group,from yellow sweet potato“Benimasari”,Ipomoea batatas Lam显示文摘 | MAOKA T AKIMOTO N ISHIGURO K | 2007 | Phytochemistry2007，68，13： | 1 |
| 19 | Recent progress in structural studies ofcarotenoids in animals and plants显示文摘 | MAOKA T | 2009 | Archives ofbiochemistry and biophysics2009，483，2： | 1 |
| 20 | Isolation of a series of apocarotenoids from the fruit of the red paprika Capsicum annuum L显示文摘 | Maoka T Fujiwara Y Hashimoto K | 2001 | J Agric Food Chem2001，49，： | 1 |
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