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| 1 | Key residues of the receptor binding motif in the spike protein of SARS-CoV-2 that interact with ACE2 and neutralizing antibodies显示文摘Coronavirus disease 2019(COVID-19),caused by the novel human coronavirus SARS-CoV-2,is currently a major threat to public health worldwide.The viral spike protein binds the host receptor angiotensin-converting enzyme 2(ACE2)via the receptor-binding domain(RBD),and thus is believed to be a major target to block viral entry.Both SARS-CoV-2 and SARS-CoV share this mechanism.Here we functionally analyzed the key amino acid residues located within receptor binding motif of RBD that may interact with human ACE2 and available neutralizing antibodies.The in vivo experiments showed that immunization with either the SARS-CoV RBD or SARS-CoV-2 RBD was able to induce strong clade-specific neutralizing antibodies in mice;however,the cross-neutralizing activity was much weaker,indicating that there are distinct antigenic features in the RBDs of the two viruses.This finding was confirmed with the available neutralizing monoclonal antibodies against SARS-CoV or SARS-CoV-2.It is worth noting that a newly developed SARS-CoV-2 human antibody,HA001,was able to neutralize SARS-CoV-2,but failed to recognize SARS-CoV.Moreover,the potential epitope residues of HA001 were identified as A475 and F486 in the SARS-CoV-2 RBD,representing new binding sites for neutralizing antibodies.Overall,our study has revealed the presence of different key epitopes between SARS-CoV and SARSCoV-2,which indicates the necessity to develop new prophylactic vaccine and antibody drugs for specific control of the COVID-19 pandemic although the available agents obtained from the SARS-CoV study are unneglectable. | Chunyan Yi Xiaoyu Sun Jing Ye Longfei Ding Meiqin Liu Zhuo Yang Xiao Lu Yaguang Zhang Liyang Ma Wangpeng Gu Aidong Qu Jianqing Xu Zhengli Shi Zhiyang Ling Bing Sun | 2020 | Cellular & Molecular Immunology2020，17，6： | 13 |
| 2 | Optimized design and test for a pendulum suspension of the crop spray boom in dynamic conditions based on a six DOF motion simulator显示文摘Spray deposit distribution from a field sprayer is mainly affected by the boom movements when the tractor is driven over a rough soil surface,the pendulum suspension that used to reduce and control the movement of spray boom by isolating the boom from vibrations of the tractor will directly enhance uniform deposition of chemicals.However,how to match the parameters of the suspension with the properties of the boom is the key problem.The dynamic rigid-flexible coupling model of the virtual prototype of the spray boom suspension system was established by using ADAMS and ABAQUS software.An optimization of the suspension parameters for a large spay boom was carried out based on the optimal Latin hypercube design,radial basis function neural network,and multi-objective genetic algorithm NSGA-II.After modified parameters of the suspension,the travel of the sprayer on a typical field motion track was simulated based on a six DOF motion simulator,and the dynamic behavior of the boom suspension was measured.The results show that RMS of the measured boom roll angle and the boom center displacement for optimized solution were reduced by 14.76%and 12.43%compared with the original suspension.Finally,the inertial measurement unit(IMU)was used to measure the movements of the sprayer vehicle during the pesticide application on the Hongze Lake Farm,the experiment of field condition reproduced by using the six DOF motion simulator,the standard deviation of the roll angle and vibration displacement for the optimized sprayer boom are only 0.6382°and 62.279 mm respectively.The research provides theoretical basis and experimental method for parameter optimization of large scale boom suspension. | Longfei Cui Hanping Mao Xinyu Xue Suming Ding Baiyu Qiao | 2018 | International Journal of Agricultural and Biological Engineering2018，11，3： | 9 |
| 3 | Design and experiment of electro hydraulic active suspension for controlling the rolling motion of spray boom显示文摘Boom sprayer is one of the most commonly used plant protection machinery for spraying pesticide.Studies have shown that the efficiency of chemicals is highly correlated with the uniformity of spray distribution patterns.As the boom is a large and flexible structure,boom rolling leads to overlapping and leakage of the pesticides.In order to improve spray uniformity,the boom attitude should be kept parallel to the ground slope or to the crop canopy beneath the boom.Passive suspension can attenuate frequencies above its resonance frequency,but nothing can be done to align the boom to the sloping ground.Therefore,an active suspension system is designed,which includes DSP-based controller,a servo valve,a hydraulic cylinder,two ultrasonic sensors,one inertial attitude sensor,and the developed control procedures.In order to prevent the wrong response of the control system caused by the high frequency component due to uneven crop canopy or rough ground.A special signal processing algorithm was proposed,including the limiting filter,smoothing algorithm and data fusion algorithm based on optimal weight.The transient and steady-state performances of the boom control system using velocity feedforward PID algorithm were tested on a six DOF motion simulator.It can be seen that the low-frequency tracking performance of the boom was greatly improved after the electro-hydraulic active suspension was added.At the resonance frequency,the peak angle of active suspension and passive suspensions are 0.72°and 1.29°respectively,and the resonance peak is greatly reduced.The controller was implemented on a self-propelled boom sprayer and validated under field conditions,the standard deviation of the roll angle of the boom with active suspension is 0.40°,compared with 1.04°of the sprayer chassis.Experimental results show that the active suspension control system can effectively reduce the effect of ground excitation disturbance on the application process,and has good tracking performance for low frequency terrain change. | Longfei Cui Xinyu Xue Feixiang Le Hanping Mao Suming Ding | 2019 | International Journal of Agricultural and Biological Engineering2019，12，4： | 3 |
| 4 | Piezo-phototronic and pyro-phototronic effects to enhance Cu(In, Ga)Se2 thin film solar cells显示文摘 | Laipan Zhu Pei Lin Baodong Chen Longfei wang Libo Chen Ding Li Zhong Lin Wang | 2018 | Nano Research2018，11，7： | 2 |
| 5 | The Crosstalk of mTOR/S6K1 and Hedgehog Pathways显示文摘 | Yan Wang Qingqing Ding Chia-Jui Yen Weiya Xia Julie G. Izzo Jing-Yu Lang Chia-Wei Li Jennifer L. Hsu Stephanie A. Miller Xuemei Wang Dung-Fang Lee Jung-Mao Hsu Longfei Huo Adam M. LaBaff Dongping Liu Tzu-Hsuan Huang Chien-Chen Lai Fuu-Jen Tsai Wei-Chao Ch | 2012 | Cancer Cell2012，，3： | 2 |
| 6 | A multiplex reverse transcription-PCR assay for the detection of influenza A virus and differentiation of the HI, H3, H5 and H9 subtypes 显示文摘 | Wu Ling Ding Longfei Pei Zenglin | 2013 | J Virol Methods2013，188，12： | 1 |
| 7 | Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry显示文摘Drug adulteration and contamination are serious threats to human health therefore,their accurate monitoring is very important.Allopurinol(Alp)and theophylline(Thp)are commonly used drugs for the treatment of gout and bronchitis,while their isomers hypoxanthine(Hyt)and theobromine(Thm)have no effect and affect the efficacy of the drug.In this work,the drug isomers of Alp/Hyt and Thp/Thm are simply mixed withα-,β-,γ-cyclodextrin(CD)and metal ions and separated using trapped ion mobility spectrometry-mass spectrometry(TIMS-MS).TIMS-MS results showed that Alp/Hyt and Thp/Thm isomers could interact with CD and metal ions and form corresponding binary or ternary complexes to achieve their TIMS separation.Different metal ions and CDs showed different separation effect for the isomers,among which Alp and Hyt could be successfully distinguished from the complexes of[Alp/Hyt+γ-CD+Cu–H]^(+)with separation resolution(RP–P)of 1.51;whereas Thp and Thm could be baseline separated by[Thp/Thm+γ-CD+Ca–H]^(+)with RP–P of 1.96.Besides,chemical calculations revealed that the complexes were in the inclusion forms,and microscopic interactions were somewhat different,making their mobility separation.Moreover,relative and absolute quantification was investigated with an internal standard to determine the precise isomers content,and good linearity(R^(2)>0.99)was obtained.Finally,the method was applied for the adulteration detection where different drugs and urine were analyzed.In addition,due to the advantages of fast speed,simple operation,high sensitivity,and no chromatographic separation required,the proposed method provides an effective strategy for the drug adulteration detection of isomers. | Zhigang Liang Huanhuan Wang Fangling Wu Longfei Wang Chenwei Li Chuan-Fan Ding | 2023 | Journal of Pharmaceutical Analysis2023，13，3： | 0 |
| 8 | Predicting the propensity for thermally activated β events in metallic glasses via interpretable machine learning显示文摘The elementary excitations in metallic glasses(MGs),i.e.,β processes that involve hopping between nearby sub-basins,underlie many unusual properties of the amorphous alloys.A high-efficacy prediction of the propensity for those activated processes from solely the atomic positions,however,has remained a daunting challenge.Recently,employing well-designed site environment descriptors and machine learning(ML),notable progress has been made in predicting the propensity for stress-activated β processes(i.e.,shear transformations)from the static structure. | Qi Wang Jun Ding Longfei Zhang Evgeny Podryabinkin Alexander Shapeev Evan Ma | 2020 | npj Computational Materials2020，，1： | 0 |
| 9 | A non-human primate derived anti-P-selectin glycoprotein ligand-1 antibody curtails acute pancreatitis by alleviating the inflammatory responses显示文摘Acute pancreatitis(AP)is a devastating disease characterized by an inflammatory disorder of the pancreas.P-selectin glycoprotein ligand-1(PSGL-1)plays a crucial role in the initial steps of the adhesive at process to inflammatory sites,blockade of PSGL-1 might confer potent anti-inflammatory effects.In this study,we generated two non-human primate derived monoclonal antibodies capable of efficiently targeting human PSGL-1,RH001-6 and RH001-22,which were screened from immunized rhesus macaques.We found that RH001-6,can effectively block the binding of P-selectin to PSGL-1,and abolish the adhesion of leukocytes to endothelial cells in vitro.In vivo,we verified that RH001-6 relieved inflammatory responses and pancreatic injury in both caerulein and L-arginine induced AP models.We also evaluated the safety profile after RH001-6 treatment in mice,and verified that RH001-6 did not cause any significant pathological damages in vivo.Taken together,we developed a novel non-human primate derived PSGL-1 blocking antibody with high-specificity,named RH001-6,which can interrupt the binding of PSGL-1 and P-selectin and attenuate inflammatory responses during AP.Therefore,RH001-6 is highly potential to be further developed into therapeutics against acute inflammatory diseases,such as AP. | Yuhan Li Xiangqing Ding Xianxian Wu Longfei Ding Yuhui Yang Xiaoliang Jiang Xing Liu Xu Zhang Jianrong Su Jianqing Xu Zhiwei Yang | 2023 | Acta Pharmaceutica Sinica B2023，13，11： | 0 |
| 10 | Design and implementation of a nonlinear robust controller based on the disturbance observer for the active spray boom suspension显示文摘Spray boom vibrations are one of the main causes of the uneven distribution of agrochemicals.Using active suspension to maintain the correct height of nozzles is critical for obtaining a uniform spray pattern and minimizing the possibility of spray drift.However,the electro-hydraulic active pendulum boom suspension has nonlinear uncertain factors such as parameter uncertainties,external disturbances,model error,etc.,which complicate the design of the controller.Therefore,this paper proposes a nonlinear robust feedback control method with disturbances compensation,which integrates a robust controller and disturbance observers through the backstepping method.Initially,to verify the performance of the controller,the Lyapunov stability theory is used to prove that the proposed controller can guarantee the given transient performance and the final tracking accuracy.Furthermore,taking the active suspension of a 28 m wide boom driven by a single-rod hydraulic actuator as an implementation case,the proposed NRCDC controller was compared with a variety of control schemes through a rapid control prototype of a pendulum active suspension.Finally,the proposed control scheme is implemented on a self-propelled sprayer with a boom of 12 m in length.The field test results show that all the performance indicators of the NRCDC controller are better than the other three conventional controllers.Both laboratory and field tests have verified the effectiveness and high performance of the proposed controller. | Longfei Cui Xinyu Xue Wei Kong Suming Ding Wei Gu Feixiang Le | 2023 | International Journal of Agricultural and Biological Engineering2023，16，1： | 0 |
| 11 | Human IFN-k Inhibited Respiratory RNA Virus Replication Dependent on Cell-to-Cell Interaction in the Early Phase显示文摘Background:Interferon kappa(IFN-k)is a type I interferon(IFN-I)that inhibits virus replication by evoking interferon-stimulated genes(ISGs).However,as an evolutionarily ancient interferon,IFN-k may function differently from the later emerged interferon-a and b.Methods:Conventional molecular biology methods were used to determine the localization of IFN-k and its structure and function.In addition,we employed RT-PCR,western blot,and RNA-Seq technologies to characterize the ISGs expression profile and antiviral activities exerted by IFN-k or IFN-a2.Results:Human IFN-k exists in two forms upon ectopic expression,one located on the cell membrane and the other secreted outside the cells.The membrane-anchored IFN-k showed the ability to induce ISGs and curtail RNA virus replication,whereas the secreted IFN-k failed to do so.Structural analyses indicated that 1-27aa at the N-terminus was the signal peptide,and 28-37aa was predicted as the transmembrane region.However,our data demonstrated that both of them were not associated with membrane localization of IFN-k;the former influenced the expression and secretion of IFN-k,and the latter had an impact on the induction of ISGs.In addition,prokaryotic purified soluble mature human IFN-k was also capable of inducing ISGs and inhibiting RNA virus replication.Importantly,human IFN-k induced a faster ISG response but with a lower intensity and a shorter half-life than the response of IFN-a2.In contrast,IFN-a2 started to function later but was stronger and more durable than IFN-k.Conclusions:Human IFN-k-induced ISG response and inhibited respiratory RNA virus replication dependent on cell-to-cell interactions.In addition,compared with IFN-a2,IFN-k exerted effects more rapidly in the early phase,with less intensity and a shorter half-life.Therefore,IFN-k may constitute the first line of IFN-I against respiratory virus infections. | Weihui Fu Peng Sun Jun Fan Longfei Ding Songhua Yuan Guanxing Zhai Miaomiao Zhang Chenli Qiu Shuye Zhang Xiaoyan Zhang Jianqing Xu | 2022 | Infectious Diseases & Immunity2022，2，2： | 0 |
| 12 | Dual-sized hollow particle incorporated fibroin thermal insulating coatings on catheter for cerebral therapeutic hypothermia显示文摘Selective endovascular hypothermia has been used to provide cooling-induced cerebral neuroprotection,but current catheters do not support thermally-insulated transfer of cold infusate,which results in an increased exit temperature,causes hemodilution,and limits its cooling efficiency.Herein,air-sprayed fibroin/silica-based coatings combined with chemical vapor deposited parylene-C capping film was prepared on catheter.This coating features in dual-sized-hollow-microparticle incorporated structures with low thermal conductivity.The infusate exit temperature is tunable by adjusting the coating thickness and infusion rate.No peeling or cracking was observed on the coatings under bending and rotational scenarios in the vascular models.Its efficiency was verified in a swine model,and the outlet temperature of coated catheter(75μm thickness)was 1.8-2.0◦C lower than that of the uncoated one.This pioneering work on catheter thermal insulation coatings may facilitate the clinical translation of selective endovascular hypothermia for neuroprotection in patients with acute ischemic stroke. | Ming Li Yuan Gao Miaowen Jiang Hongkang Zhang Yang Zhang Yan Wu Wenhao Zhou Di Wu Chuanjie Wu Longfei Wu Luzi Bao Xiaoxiao Ge Zhengfei Qi Ming Wei Ang Li Yuchuan Ding Jicheng Zhang Guangzhen Pan Yu Wu Yan Cheng Yufeng Zheng Xunming Ji | 2023 | Bioactive Materials2023，，8： | 0 |
| 13 | Piezoelectric Zinc Oxides with High Polar Facets Ratios for Mechanically Controlled RAFT Polymerization显示文摘Mechanoredox chemistry that uses highly polarized piezoelectric materials as mechanoredox catalysts to promote redox reactions has emerged recently.It provides an alternative approach alongside the existing polymerization methods.Despite recent accomplishments,determining the quantitative relationship between the structure of ZnO and its catalytic performance for polymerization is still challenging.Herein,we prepared various ZnO crystals with different polar facets ratios to achieve efficient mechanically induced reversible addition-fragmentation chain transfer polymerization(mechano-RAFT).ZnO prepared from Zn(NO3)2 showed a high polar facet ratio of 1.66 and offered the highest catalytic activity among all ZnO samples.A near-quantitative initiator efficiency of 99.5%and narrow molecular weight distribution were achieved for the polymerization of n-butyl acrylate.Furthermore,the high chain-end fidelity and chain extension capability were also evidenced by MALDI-TOF MS and GPC analysis.This work highlighted the significant contribution of polar facets in ZnO to its catalytic activity and will guide the design of mechanoredox catalysis with superior catalytic performance in the future. | Chengqiang Ding Ziye Ren Jian Wang Longfei Zhang Yuhan Yan Danming Wu Zhao Wang Zhengbiao Zhang | 2023 | Chinese Journal of Chemistry2023，41，20： | 0 |
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