| 1 | 干旱胁迫的水稻根高效酵母双杂交体系建立显示文摘采用SMART技术在酵母菌株AH109中构建了干旱胁迫下水稻根部全长cDNA文库,采用改进的滤膜杂交方法建立了高效酵母杂交体系,以抗旱相关水稻转录调控因子OsWRKY71基因作为诱饵对该方法进行检验。实验获得的酵母杂交文库容量为4.9×106,平均插入片段为800bp,达到了基因分离和克隆等后续研究的标准。同时采用滤膜杂交法将文库的杂交效率提高到了6.9%,是液体杂交法的6倍以上。该方法不需要特殊的设备,且具有低消耗和高效率的特点,可用于高通量酵母双杂交分析。 | 付坚 Linkun GU 郭怡卿 Liyuan ZHANG 王玲仙 李定琴 王波 Jeff Qingxi SHEN 程在全 | 2013 | 中国水稻科学2013，27，2： | 2 |
| 2 | Collaborative Offloading Method for Digital Twin Empowered Cloud Edge Computing on Internet of Vehicles显示文摘Digital twinning and edge computing are attractive solutions to support computing-intensive and servicesensitive Internet of Vehicles applications.Most of the existing Internet of Vehicles service offloading solutions only consider edge–cloud collaboration,but the collaboration between small cell eNodeB(SCeNB)should not be ignored.Service delays far lower than offloading tasks to the cloud can be obtained through reasonable collaborative computing between nodes.The proposed framework realizes and maintains the simulation of collaboration between SCeNB nodes by constructing a digital twin that maintains SCeNB nodes in the central controller,thereby realizing user task offloading positions,sub-channel allocation,and computing resource allocation.Then an algorithm named AUC-AC is proposed,based on the dominant actor–critic network and the auction mechanism.In order to obtain a better command of global information,the convolutional block attention mechanism(CBAM)is used in the digital twin of each SCeNB node to observe its environment and learn strategies.Numerical results show that our experimental scheme is better than several baseline algorithms in terms of service delay. | Linjie Gu Mengmeng Cui Linkun Xu Xiaolong Xu | 2023 | Tsinghua Science and Technology2023，28，3： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员