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| 1 | MiR-375 frequently downregulated in gastric cancer inhibits cell proliferation by targeting JAK2显示文摘新兴的证据与肿瘤开发和前进显示出异常地表示的 microRNAs (miRNAs ) 的协会。然而,很少在胃的 carcinogenesis 对 miRNAs 的潜在的角色被知道。这里,我们执行了 miRNA microarray 屏蔽在配对的胃的癌症和他们的邻近的 nontumor 纸巾表示并且发现 miR-375 是的 miRNAs 差别极大地在胃的癌症纸巾的 downregulated。量的即时 PCR 分析证实那 miR-375 表情显著地从经历胃的切除术的病人与他们的 nontumor 对应物相比在超过 90% 主要胃的癌症被减少。miR-375 的 Overexpression 显著地在 vitro 并且在 vivo 禁止了胃的癌症房间增长。在胃的癌症房间的 miR-375 的强迫的表示显著地减少了 Janus kinase 的蛋白质水平 2 ( JAK2 )并且镇压带 JAK2 的 3 鈥? untranslated 区域的一个酶记者的活动,被预言的 miR-375-binding 地点的变化废除,显示那 JAK2 可以是 miR-375 目标基因。由由 RNAi 的 JAK2 的 AG490 或 silencing 的 JAK2 活动的任何一个抑制压制了类似于 miR-375 overexpression 的胃的癌症房间增长。而且, JAK2 的宫外的表示能部分颠倒 miR-375 引起的房间增长的抑制。最后,我们在胃的癌症发现了在 miR-375 表示和 JAK2 蛋白质水平之间的重要反的关联。因此,这些数据建议 miR-375 可以作为肿瘤 suppressor 工作由指向 JAK2 oncogene 潜在地调整胃的癌症房间增长,含有在胃的癌症的致病的 miR-375 的一个角色。 | Ling Ding Yanjun Xu Wei Zhang Yujie Deng Misi Si Ying Du Haomi Yao Xuyan Liu Yuehai Ke Jianmin Si Tianhua Zhou | 2010 | Cell Research2010，20，7： | 59 |
| 2 | Induction of the LRP16 gene by estrogen promotes the invasive growth of Ishikawa human endometrial cancer cells through the downregulation of E-cadherin显示文摘LRP16 以前在乳癌房间作为导致雌激素的基因被识别。到在子宫内膜的癌症(EC ) 的雌激素和它的功能的效果的 LRP16 的应答的海角房间仍然是不清楚的。这里,我们证明 LRP16 基因的信使 rna 水平和倡导者活动被 17beta-estradiol (E2 ) 显著地在雌激素受体高山增加哈(嗯高山哈)-positiveIshikawa 人 EC 房间。尽管 Ishikawa 细胞的生长率没被 LRP16 的宫外的表示显然影响, Transwell 试金的结果显示出 LRP16-overexpressing 细胞的侵略能力的近似 one-thirdincrease。由于分子的屏蔽,我们观察到 E-cadherin 的表示,与肿瘤转移联系的一个必要粘附分子,被 LRP16 镇压。进一步的倡导者分析以一种剂量依赖者方式表明了那 LRP16 inhibitedE-cadherin transactivation。然而,抑制被雌激素剥夺废除,显示由 LRP16requires 的 E-cadherin 抄写的 down 规定嗯高山哈调停。染色质免疫降水分析表明到 E-cadherin 倡导者的 ERalpha 的绑定被 LRP16 反对,建议那 LRP16 能防碍嗯调停 alpha 的抄写。这些结果建议起来由雌激素的 LRP16 的规定能被在人的 EC 调整 E-cadherin 的 down 涉及侵略生长。 | Yuan Guang Meng Wei Dong Han Ya Li Zhao Ke Huang Yi Ling Si Zhi Qiang Wu Yi Ming Mu | 2007 | Cell Research2007，17，10： | 27 |
| 3 | Expressions of ICAM-1 and its mRNA in sera and tissues of patients with hepatocellular carcinoma显示文摘INTRODUCTIONThe increased expression of ICAM-1 on a widerange of cells and in the sera of patients withmalignancies, chronic liver diseases andinflammation diseases has been described since thelate 1980s[1-22]. Recently rapid progress in studieson expression of ICAM-1 in patients withhepatocellular carcinoma ( HCC ) have beenachieved, including clinical and experimentalresearches[23-31]. | Jing Xu1 Ming Hui Mei1 Si En Zeng2 Qing Fen Shi3 Yong Ming Liu4 Li Ling Qin3 1Department of Hepatobiliary Surgery2Department of Pathology3Institute of Hepatobiliary Surgery4Department of Biochemistry, Guilin 541001, Guangxi Province, China | 2001 | World Journal of Gastroenterology2001，7，1： | 14 |
| 4 | Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells显示文摘Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy(CADASIL)is a rare hereditary cerebrovascular disease caused by a NOTCH3 mutation.However,the underlying cellular and molecular mechanisms remain unidentified.Here,we generated non-integrative induced pluripotent stem cells(iPSCs)from fibroblasts of a CADASIL patient harboring a heterozygous NOTCH3 mutation(c.3226C>T,p.R1076C).Vascular smooth muscle cells(VSMCs)differentiated from CADASIL-specific iPSCs showed gene expression changes associated with disease phenotypes,including activation of the NOTCH and NF-kB signaling pathway,cytoskeleton disorganization,and excessive cell proliferation.In comparison,these abnormalities were not observed in vascular endothelial cells(VECs)derived from the patients iPSCs.Importantly,the abnormal upregulation of NF-kB target genes in CADASIL VSMCs was diminished by a NOTCH pathway inhibitor,providing a potential therapeutic strategy for CADASIL.Overall,using this iPSCbased disease model,our study identified clues for studying the pathogenic mechanisms of CADASIL and developing treatment strategies for this disease. | Chen Ling Zunpeng Liu Moshi Song Weiqi Zhang Si Wang Xiaoqian Liu Shuai Ma Shuhui Sun Lina Fu Qun Chu Juan Carlos Izpisua Belmonte Zhaoxia Wang Jing Qu Yun Yuan Guang-Hui Liu | 2019 | Protein & Cell2019，10，4： | 7 |
| 5 | Comparative genomic and transcriptomic analyses uncover the molecular basis of high nitrogen-use efficiency in the wheat cultivar Kenong 9204显示文摘Studying the regulatory mechanisms that drive nitrogen-use efficiency(NUE)in crops is important for sustainable agriculture and environmental protection.In this study,we generated a high-quality genome assembly for the high-NUE wheat cultivar Kenong 9204 and systematically analyzed genes related to nitrogen uptake and metabolism.By comparative analyses,we found that the high-affinity nitrate transporter gene family had expanded in Triticeae.Further studies showed that subsequent functional differentiation endowed the expanded family members with saline inducibility,providing a genetic basis for improving the adaptability of wheat to nitrogen deficiency in various habitats.To explore the genetic and molecular mechanisms of high NUE,we compared genomic and transcriptomic data from the high-NUE cultivar Kenong 9204(KN9204)and the low-NUE cultivar Jing 411 and quantified their nitrogen accumulation under high-and low-nitrogen conditions.Compared with Jing 411,KN9204 absorbed significantly more nitrogen at the reproductive stage after shooting and accumulated it in the shoots and seeds.Transcriptome data analysis revealed that nitrogen deficiency clearly suppressed the expression of genes related to cell division in the young spike of Jing 411,whereas this suppression of gene expression was much lower in KN9204.In addition,KN9204 maintained relatively high expression of NPF genes for a longer time than Jing 411 during seed maturity.Physiological and transcriptome data revealed that KN9204 was more tolerant of nitrogen deficiency than Jing 411,especially at the reproductive stage.The high NUE of KN9204 is an integrated effect controlled at different levels.Taken together,our data provide new insights into the molecular mechanisms of NUE and important gene resources for improving wheat cultivars with a higher NUE trait. | Xiaoli Shi Fa Cui Xinyin Han Yilin He Long Zhao Na Zhang Hao Zhang Haidong Zhu Zhexin Liu Bin Ma Shusong Zheng Wei Zhang Jiajia Liu Xiaoli Fan Yaoqi Si Shuiquan Tian Jianqing Niu Huilan Wu Xuemei Liu Zhuo Chen Deyuan Meng Xiaoyan Wang Liqiang Song Lijing Sun Jie Han Hui Zhao Jun Ji Zhiguo Wang Xiaoyu He Ruilin Li Xuebin Chi Chengzhi Liang Beifang Niu Jun Xiao Junming Li Hong-Qing Ling | 2022 | Molecular Plant2022，15，9： | 6 |
| 6 | S100A8 promotes epithelial-mesenchymal transition and metastasis under TGF-β/USF2 axis in colorectal cancer显示文摘Background:The transforming growth factor-β(TGF-β)pathway plays a pivotal role in inducing epithelial-mesenchymal transition(EMT),which is a key step in cancer invasion and metastasis.However,the regulatory mechanism of TGF-βin inducing EMT in colorectal cancer(CRC)has not been fully elucidated.In previous studies,it was found that S100A8 may regulate EMT.This study aimed to clarify the role of S100A8 in TGF-β-induced EMT and explore the underlying mechanism in CRC.Methods:S100A8 and upstream transcription factor 2(USF2)expression was detected by immunohistochemistry in 412 CRC tissues.Kaplan-Meier survival analysis was performed.In vitro,Western blot,and migration and invasion assays were performed to investigate the effects of S100A8 and USF2 on TGF-β-induced EMT.Mouse metastasis models were used to determine in vivo metastasis ability.Luciferase reporter and chromatin immunoprecipitation assay were used to explore the role of USF2 on S100A8 transcription.Results:During TGF-β-induced EMT in CRC cells,S100A8 and the transcription factor USF2 were upregulated.S100A8 promoted cell migration and invasion and EMT.USF2 transcriptionally regulated S100A8 expression by directly binding to its promoter region.Furthermore,TGF-βenhanced the USF2/S100A8 signaling axis of CRC cells whereas extracellular S100A8 inhibited the USF2/S100A8 axis of CRC cells.S100A8 expression in tumor cells was associated with poor overall survival in CRC.USF2 expression was positively related to S100A8 expression in tumor cells but negatively related to S100A8-positive stromal cells.Conclusions:TGF-βwas found to promote EMT and metastasis through the USF2/S100A8 axis in CRC while extracellular S100A8 suppressed the USF2/S100A8 axis.USF2 was identified as an important switch on the intracellular and extracellular S100A8 feedback loop. | Si Li Jun Zhang Senmi Qian Xuesong Wu Liang Sun Tianyi Ling Yao Jin Wenxiao Li Lichao Sun Maode Lai Fangying Xu | 2021 | Cancer Communications2021，41，2： | 5 |
| 7 | Apoptosis of pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid in combination with horseradish peroxidase显示文摘AIM: To explore the mechanisms underlying the apoptosis of human pancreatic cancer BXPC-3 cells induced by indole-3-acetic acid (TAA) in combination with horseradish peroxidase (HRP).METHODS: BXPC-3 cells derived from human pancreatic cancer were exposed to 40 or 80 μmol/L IAA and 1.2 μg/mL HRP at different times. Then, MTT assay was used to detect the cell proliferation. Flow cytometry was performed to analyze cell cycle. Terminal deoxynucleotidyl transferasemediated dUTP nick end labeling assay was used to detect apoptosis. 2,7-Dichlorofluorescin diacetate uptake was measured by confocal microscopy to determine free radicals. Level of malondialdehyde (MDA) and activity of superoxide dismutase (SOD) were measured by biochemical methods.RESULTS: IAA/HRP initiated growth inhibition of BXPC-3 cells in a dose- and time-dependent manner. Flow cytometry revealed that the cells treated for 48 h were arrested at G1/G0. After exposure to 80 μmol/L IAA plus 1.2 μg/mL HRP for 72 h, the apoptosis rate increased to 72.5‰,which was nine times that of control. Content of MDA and activity of SOD increased respectively after treatment compared to control. Meanwhile, IAA/HRP stimulated the formation of free radicals.CONCLUSION: The combination of IAA and HRP can inhibit the growth of human pancreatic cancer BXPC-3 cells in vitro by inducing apoptosis. | Chen Huang Li-Ying Liu Tu-Sheng Song Lei Ni Ling Yang Xiao-Yan Hu Jing-Song Hu Li-Ping Song Yu Luo Lu-Sheng Si | 2005 | World Journal of Gastroenterology2005，11，29： | 5 |
| 8 | Efficacy and economic analysis of Ex- PRESS implantation versus trabeculectomy in uncontrolled glaucoma: a systematic review and Meta-analysis显示文摘AIM:To systematically review the current evidence based on the efficacy and cost of Ex-PRESS implantation and trabeculectomy(Trab)for uncontrolled glaucoma.·METHODS:Clinical trials were identified by electronic databases(Pub Med,EMBASE,ISI Web of science and Cochrane library),and data,such as intraocular pressure(IOP),the complete and qualified success rate,the postoperative complications and the cost,were exacted from these relevant studies.Weighted mean difference(WMD),odds ratio(OR)and 95%confidence intervals(CIs)were calculated and were pooled using a randomeffects model.·RESULTS:Eleven relevant publications and two abstracts met the inclusion criteria.The efficacy of ExPRESS was similar to that of Trab in the percentage of IOP reduction(IOPR%)at 1,2y(WMD:-2.01;95%CI:-7.92-3.90;P=0.50 and WMD:2.89;95%CI:-8.05-13.83;P=0.60,respectively).Ex-PRESS possessed a significant higher complete and qualified success rate(OR:1.59;95%CI:1.07-2.35;P=0.02 and OR:1.74;95%CI:1.06-2.86;P=0.03,respectively).Moreover,Ex-PRESS exerted a significantly lower frequency of hypotony and hyphema than Trab(OR:0.39;95%CI:0.21-0.72;P=0.003 and OR:0.27;95%CI:0.10-0.69;P=0.003,respectively).However,there was no consistent result on the cost between the two groups according to the previous three studies.·CONCLUSION:Both Trab and Ex-PRESS have equivalent efficacy in lowering IOP,yet Ex-PRESS had a lower risk of hypotony and hyphema than Trab.Nevertheless,whether the cost of Ex-PRESS was less than that of Trab should be further investigated to ensure evidence-based conclusion in the long run. | Ling Wang Fang Sha Da-Dong Guo Hong-Sheng Bi Jun-Kang Si Yu-Xiang Du Kai Tang | 2016 | International Journal of Ophthalmology(English edition)2016，9，1： | 5 |
| 9 | Association between intestinal microbiota and inflammatory bowel disease显示文摘Inflammatory bowel disease(IBD),which includes Crohn’s disease(CD)and ulcerative colitis(UC),has emerged as a global disease with high incidence,long duration,devastating clinical symptoms,and low curability(relapsing immune response and barrier function defects).Mounting studies have been performed to investigate its pathogenesis to provide an ever-expanding arsenal of therapeutic options,while the precise etiology of IBD is not completely understood yet.Recent advances in highthroughput sequencing methods and animal models have provided new insights into the association between intestinal microbiota and IBD.In general,dysbiosis characterized by an imbalanced microbiota has been widely recognized as a pathology of IBD.However,intestinal microbiota alterations represent the cause or result of IBD process remains unclear.Therefore,more evidences are needed to identify the precise role of intestinal microbiota in the pathogenesis of IBD.Herein,this review aims to outline the current knowledge of commonly used,chemically induced,and infectious mouse models,gut microbiota alteration and how it contributes to IBD,and dysregulated metabolite production links to IBD pathogenesis. | Yunchang Zhang Xuemeng Si Ling Yang Hui Wang Ye Sun Ning Liu | 2022 | Animal Models and Experimental Medicine2022，5，4： | 3 |
| 10 | Prevention of Osteopenia and Dyslipidemia in Rats after Ovariectomy with Combined Aspirin and Low-dose Diethylstilbestrol显示文摘Objective To study whether effect of aspirin plus low-dose diethylstilbestrol is more effective and safer than high diethylstilbestrol dose alone on prevention of ovariectomy-induced osteopenia and dyslipidemia.Methods Thirty-eight 4-month-old female SD rats were divided into baseline(BAS)group(n=6),sham operation group(n=8)and ovariectomy(OVX)group(n=24).The OVX group was further divided into vehicle treatment group(n=8),diethylstilbestrol(30 μg/kg·d)treatment group(OVX+D30 group,n=8),and aspirin(9 mg/kg·d)plus diethylstilbestrol(10 μg/kg·d)treatment group(OVX+A-D10 group,n=8).Their left tibiae were collected for the bone histomorphometric analysis in undecalcified sections.Left femurs were collected for the bone mineral density measurement.Results The body weight and serum cholesterol were increased,while uterine weight and cancellous bone mass were decreased in OVX rats compared with the SHAM group.Cancellous bone mass was significantly increased,while body weight and bone resorption parameters were decreased in both A-D10 and D30 treatment group compared with OVX group.The rats treated with A-D10 showed significantly increased in bone formation parameters and decreased in serum triglyceride compared with the D30-treated rats.Conclusion Aspirin plus low-dose diethylstilbestrol can effectively prevent osteopenia by reducing bone resorption,and is thus a better treatment modality for preventing dyslipidemia than high-dose diethylstilbestrol alone. | LIN Si En HUANG Jian Ping WU Ling Zhi WU Tie CUI Liao | 2013 | Biomedical and Environmental Sciences2013，26，4： | 3 |
| 11 | Flexible Three-Dimensional Graphene Hydrogels with Superior Conductivity and Excellent Electrochemical Performance for Supercapacitor Electrodes显示文摘三维的多孔的 graphene hydrogels 作为一个助手由一块草地和灵巧却很有效的途径使用葡萄糖被准备了。与象反应时间和温度那样的最佳的试验性的条件把热水的反应基于一出一步舞, graphene hydrogels 展览没有任何文件夹,优异电的传导性( 95.3 S/m )和罐头被用作 supercapacitor 电极或进行添加剂,但是在 1 A/g 的当前的密度显示出 384.6 F/g 的一个高特定的电容。结果证明葡萄糖的增加不能仅仅极大地减少反应温度而且弄短反应时间。为 supercapacitor 的电极在精力存储设备的地里建议它的有希望的潜力的三维的多孔的 graphene hydrogels 的优异表演。 | Juan Zhang Bo Zhou Bo Zhao Ling Si Xiaoqing Jiang | 2017 | Chinese Journal of Chemistry2017，35，10： | 3 |
| 12 | The origin of Mosuo people as revealed by mtDNA and Y chromosome variation显示文摘The Mosuo, living in the Lugu Lake area in northwest Yunnan Province, China, is the only matriarchal population in China. The Mosuo was officially identified as Naxi nationality although its relationship with Naxi remains controversial. We studied the genetic relationship between the Mosuo and five other ethnic groups currently residing in northwest Yunnan, i.e. Naxi, Tibetan, Bai, Yi and Pumi, by typing the genetic variations in mtDNA HVS1 and 21 Y chromosome markers (13 SNPs & 8 STR markers). We showed that the maternal lineages of the Mosuo bear the strongest resemblance with those found in Naxi while its paternal lineages are more similar to those that are prevalent in Yunnan Tibetan. The marked difference between paternal and maternal lineages may be attributable to the genetic history, matriarchal structure, and visiting marriage. | WEN Bo, SHI Hong, REN Ling, XI Huifeng, LI Kaiyuan, ZHANG Wenyi, SU Bing, SI Shiheng, JIN Li & XIAO ChunjieJoined Laboratory of Human Population Genetics of Yunnan University and Fudan University, Kunming 650091, China Key Laboratory of Bioresources Conservation and Utilization & Human Genetics Center of Yunnan University, Kunming 650091, China National Key Laboratory of Genetic Engineering & Center for Anthropological Studies, School of Life Sciences, Fudan University, Shanghai 200433, China Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650031, China Center for Genome Information, Department of Environmental Health, University of Cincinnati, Cincinnati 45267, USA The Central Hospital of Ninglang County, Ninglang 674300, China | 2004 | Science China(Life Sciences)2004，47，1： | 3 |
| 13 | High-efficiency acetaldehyde removal during solid-state polycondensation of poly(ethylene terephthalate) assisted by supercritical carbon dioxide显示文摘The concentration of acetaldehyde(AA) is the main quality index of poly(ethylene terephthalate)(PET) used in food and drink packaging.A new method for AA removal has been developed by using supercritical carbon dioxide(sc CO2) during the solid-state polycondensation of PET.The influence factors of AA removal including the temperature,pressure,reaction time and the size of pre-polymer particles are systematically studied in this work.The results indicate that it is a highly efficient way to obtain high molecular weight PET with relative low concentration of AA.Correspondingly,the polymerization degree of PET could increase from 27.9 to 85.6 while the concentration of AA reduces from 0.229 × 10^(-6) to 0.055 × 10^(-6) under the optimal operation conditions of 230 °C,8 MPa and size of 0.30–0.45 mm.Thermodynamic performance tests show the increasing extent of PET crystallinity due to the fact that the plasticization of sc CO_2 is not obvious with extended reaction time,therefore the increasing crystallinity has no significant influence on AA removal.SEM observations reveal that the effects of sc CO_(2-) induced plasticization and swelling on PET increase significantly with the decrease of prepolymer size,and the surface of PET becomes more loose and porous in favor of the AA removal. | Zhenhao Xi Tian Liu Wei Si Fenglei Bi Zhimei Xu Ling Zhao | 2018 | Chinese Journal of Chemical Engineering2018，26，6： | 2 |
| 14 | 2020 Roadmap on two-dimensional nanomaterials for environmental catalysis显示文摘Environmental catalysis has drawn a great deal ofattention due to its clean ways to produce useful chemicals or carry out some chemical processes.Photocatalysis and electrocatalysis play important roles in these fields.They can decompose and remove organic pollutants from the aqueous environment,and prepare some fine chemicals.Moreover,they also can carry out some important reactions,such as 02 reduction reaction(ORR),O2 evolution reaction(OER),H2 evolution reaction(HER),CO2 reduction reaction(C02 RR),and N2 fixation(NRR).For catalytic reactions,it is the key to develop high-performance catalysts to meet the demand fortargeted reactions.In recentyears,two-dimensional(2 D) materials have attracted great interest in environmental catalysis due to their unique layered structures,which offer us to make use of their electronic and structural characteristics.Great progress has been made so far,including graphene,black phosphorus,oxides,layered double hydroxides(LDHs),chalcogenides,bismuth-based layered compounds,MXenes,metal organic frameworks(MOFs),covalent organic frameworks(COFs),and others.This content drives us to invite many famous groups in these fields to write the roadmap on two-dimensional nanomaterials for environmental catalysis.We hope that this roadmap can give the useful guidance to researchers in future researches,and provide the research directions. | Yulu Yang Mingguang Wu Xingwang Zhu Hui Xu Si Ma Yongfeng Zhi Hong Xia Xiaoming Liu Jun Pan Jie-Yinn Tang Siang-Piao Chai Leonardo Palmisano Francesco Parrino Junli Liu Jianzhong Ma Ze-Lin Wang Ling Tan Yu-Fei Zhao Yu-Fei Song Pardeep Singh Pankaj Raizada Deli Jiang Di Li R.A.Geioushy Jizhen Ma Jintao Zhang Song Hu Rongjuan Feng Gang Liu Minghua Liu Zhenhua Li Mingfei Shao Neng Li Jiahe Peng Wee-Jun Ong Nikolay Kornienko Zhenyu Xing Xiujun Fan Jianmin Ma | 2019 | Chinese Chemical Letters2019，30，12： | 2 |
| 15 | Two new steroidal glucosides from Ophiopogon japonicus (L.f.) Ker-Gawl显示文摘二新 steroidal 配糖物,26-O-尾- d-glucopyranosyl ( 25S ) -furost-5-ene-1尾,3尾,22伪,26-tetraol 1-O-尾-d-xylopyranosyl-( 1 鈫? 3 ) -[伪-l-rhamnopyranosyl-(1 鈫? 2 )] -尾-d-fucopyranoside 并且( 25R ) spirost-5-ene-3 尾, 14 伪-diol-3-尾-O-尾- l 鈥搑h amnopyranosyl ( 1 鈫? 2 ) -[尾-d-xylopyranosyl(1 鈫? 4 )]-尾-d-glucopyranoside,从 Ophiopogon japonicus ( L.f )被孤立Ker地上的低陷处。他们的结构被分光镜的方法阐明。 | Ya Jiuan Xu Tun Hai Xu Ling Zhu Hao Hong Feng Zhao Sheng Xu Xie Yun Shan Si Dong Han Dong Ming Xu | 2008 | Chinese Chemical Letters2008，19，7： | 2 |
| 16 | Spatial variability of cyanobacterial community composition in Sanya Bay as determined by DGGE fingerprinting and multivariate analysis显示文摘The cyanobacterial communities in the surface and bottom waters of Sanya Bay were investigated on April 24 and 25,2010.Flow cytometry showed that the total cyanobacterial abundance in the surface and bottom layers ranged from 0.7×10 4 to 2.38×10 4 cells mL-1 and from 1×10 4 to 1.8×10 4 cells mL-1,respectively.Cyanobacterial diversity was analyzed using a molecular fingerprinting technique called denaturing gradient gel electrophoresis(DGGE),followed by DNA sequencing.The results were then interpreted through multivariate statistical analysis.Differences in the compositions of cyanobacterial communities were observed in the surface and bottom waters at the same station,with some bands obtained from both the surface and bottom layers,whereas some bands were present only in one layer.The predominant cyanobacterial species of the excised DGGE bands were related to Synechococcus or Synechococcus-like species(56.2%).Other phylogenetic groups identified included Chroococcidiopsis(6.3%),Cyanobium(6.3%) and some unclassified cyanobacteria(31.2%).A redundancy analysis(RDA) was conducted to reveal the relationships between the cyanobacterial community composition and environmental factors.Analysis results showed that the spatial variations in the cyanobacterial community composition in surface waters was significantly related to chlorophyll a(Chla),the biochemical oxygen demand(BOD),nitrate and phosphate(P<0.05).Meanwhile,the spatial variations in the bottom waters was significantly affected by nitrate,nitrite,and phosphate(P<0.05).Environmental parameters could explain 99.3% and 58.3% of the variations in the surface and bottom layers,respectively. | LING Juan ZHANG YanYing DONG JunDe WANG YouShao HUANG Hui CHEN Lei HUANG XiaoFang LONG LiJuan ZHANG Si | 2013 | Chinese Science Bulletin2013，58，9： | 2 |
| 17 | Panda X-III: Searching for neutrinoless double beta decay with high pressure ^(136)Xe gas time projection chambers显示文摘Searching for the neutrinoless double beta decay(NLDBD)is now regarded as the topmost promising technique to explore the nature of neutrinos after the discovery of neutrino masses in oscillation experiments.Panda X-III(particle and astrophysical xenon experiment III)will search for the NLDBD of136Xe at the China Jin Ping Underground Laboratory(CJPL).In the first phase of the experiment,a high pressure gas Time Projection Chamber(TPC)will contain 200 kg,90%136Xe enriched gas operated at10 bar.Fine pitch micro-pattern gas detector(Microbulk Micromegas)will be used at both ends of the TPC for the charge readout with a cathode in the middle.Charge signals can be used to reconstruct the electron tracks of the NLDBD events and provide good energy and spatial resolution.The detector will be immersed in a large water tank to ensure~5 m of water shielding in all directions.The second phase,a ton-scale experiment,will consist of five TPCs in the same water tank,with improved energy resolution and better control over backgrounds. | Xun Chen Chang Bo Fu Javier Galan Karl Giboni Franco Giuliani Ling Hui Gu Ke Han Xiang Dong Ji Heng Lin Jiang Lai Liu Kai Xiang Ni Hiroki Kusano Xiang Xiang Ren Shao Bo Wang Yong Yang Dan Zhang Tao Zhang Li Zhao Xiang Ming Sun Shou Yang Hu Si Yu Jian Xing Long Li Xiao Mei Li Hao Liang Huan Qiao Zhang Ming Rui Zhao Jing Zhou Ya Jun Mao Hao Qiao Si Guang Wang Ying Yuan Meng Wang Amir N.Khan Neill Raper Jian Tang Wei Wang Jia Ning Dong Chang Qing Feng Cheng Li Jian Bei Liu Shu Bin Liu Xiao Lian Wang Dan Yang Zhu Juan F.Castel Susana Cebri'an Theopisti Dafni Javier G.Garza Igor G.Irastorza Francisco J.Iguaz Gloria Luz ′on Hector Mirallas Stephan Aune Eric Berthoumieux Yann Bedfer Denis Calvet Nicole d'Hose Alain Delbart Maria Diakaki Esther Ferrer-Ribas Andrea Ferrero Fabienne Kunne Damien Neyret Thomas Papaevangelou Franck Sabatie Maxence Vanderbroucke AnDi Tan Wick Haxton Yuan Mei Chinorat Kobdaj Yu-Peng Yan | 2017 | Science China(Physics,Mechanics & Astronomy)2017，60，6： | 2 |
| 18 | LMP2-DC Vaccine Elicits Specific EBV-LMP2 Response to Effectively Improve Immunotherapy in Patients with Nasopharyngeal Cancer显示文摘Objective To evaluate the safety and effectiveness of a vaccine based on latent membrane protein 2(LMP2)modified dendritic cells(DCs)that boosts specific responses of cytotoxic T lymphocytes(CTLs)to LMP2 before and after intradermal injection in patients with nasopharyngeal carcinoma(NPC).Methods DCs were derived from peripheral blood monocytes of patients with NPC.We prepared LMP2-DCs infected by recombinant adenovirus vector expressing LMP2(rAd-LMP2).NPC patients were immunized with 2×105 LMP2-DCs by intradermal injection at week 0 and after the second and fourth weeks.Specific responses to LMP2 were detected by enzyme-linked immunospot(ELISPOT)assay at week 0 and at the fifth and eighth weeks.Local clinicians performed the follow-up and tracking of patients.Results We demonstrated that DCs derived from monocytes displayed typical DC morphologies;the expression of LMP2 in the LMP2-DCs vaccine was confirmed by immunocytochemical assay.Twenty-nine patients with NPC were enrolled in this clinical trial.The LMP2-DCs vaccine was well tolerated in all of the patients.Boosted responses to LMP2 peptide sub-pools were observed in 18 of the 29 patients with NPC.The follow-up data of 29 immunized patients from April,2010 to April 2015 indicated a five-year survival rate of 94.4%in responders and 45.5%in non-responders.Conclusion In this pilot study,we demonstrated that the LMP2-DCs vaccine is safe and effective in patients with NPC.Specific CTLs responses to LMP2 play a certain role in controlling and preventing the recurrence and metastasis of NPC,which warrants further clinical testing. | ZENG Yi SI Yong Feng LAN Gui Ping WANG Zhan ZHOU Ling TANG Min Zhong SJ O`Brien LAN Jiao ZHOU Xiang Yang WANG Yong Li TANG Juan ZHOU Zhi Xiang DU Hai Jun LIN Hui | 2020 | Biomedical and Environmental Sciences2020，33，11： | 2 |
| 19 | Facile synthesis of hypercrosslinked resin via photochlorination of p-xylene and succedent alkylation polymerization显示文摘p 二甲苯和随后的 FriedelCrafts 完化聚合的 photochlorination 的联合第一在 hypercrosslinked 的准备被使用吸附的树脂。当 p 二甲苯是 photochlorinated 时, GCMS 和 GC 的数据证明一系列 chlorizates 被生产。Hypercrosslinked 树脂能被 copolymerization, chlorizates 的自我聚合或柱子 crosslinking 反应综合。多孔的树脂的化学结构和 micromorphology 被赌注,英尺红外, SEM 和基本分析(EA ) 描绘。结果证明新奇吸附的树脂从 0.5 ~ 1.2 cm3/g 近拥有高赌注表面到 1038 m2/g 和大毛孔卷范围。 | Ling Ling Bai Yong Hao Zhou Xiu Li Wang Si Guo Yuan Xian Li Wu | 2011 | Chinese Chemical Letters2011，22，9： | 2 |
| 20 | Review of randomized trials of traditional Chinesedicine 显示文摘 | Tang Jin Ling Zhan Si Yan Edzard Ernst | 1999 | BMJ1999，319，： | 1 |
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