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| 1 | Short-DNA Specific Blocker PCR for Efficient and Simple Enrichment of Cell Free Fetal DNAs with Short Lengths显示文摘As an inn ate characteristic,DNA length has attracted more and more atte ntion.The length of some import ant biomarkers such as ctDNA(Circulating tumor DNA)and cffDNA(Cell free fetal DNA)is shorter than that of cell-free DNA.Researchers have utilized the differe nee in length to in crease the abunda nee of ctDNA or cffDNA in total cell-free DNA to overcome the difficulties in detecti on due to their low abundance. | Yangwei Liao Xiaofeng Tang Zhihao Ming Lida Ren Wei Zhang Xianjin Xiao | 2021 | Chinese Journal of Chemistry2021，39，8： | 2 |
| 2 | Shared-probe system:An accurate,low-cost and general enzyme-assisted DNA probe system for detection of genetic mutation显示文摘Enzyme assisted DNA probes are powerful tools in molecular diagnostics for their simplicity,rapidity,and low detection limit.However,cost of probes,difficulty in optimization and disturbance of secondary structure hindered the wider application of enzyme assisted DNA probes.To solve the problems,we designed a new system named shared-probe system.By introducing two unlabeled single stranded DNA named Sh1 and Sh2 as the bridge between probe and the substrate,the same sequence of dually labeled probe with stable performance was shared for different mutations,thus sparing the expense and time cost on designing,synthesizing and optimizing corresponding probes.Besides,the hybridization between Sh1 and the substrate could overcome secondary structures,which guaranteed the detection of different substrates.The performance and generality of the design were tested by low abundance detection in synthetic single DNA samples and the limit of detection was 0.05%for PTENR130 Q,EGFR-L858 R and 0.02%for BRCA1-NM007294.3.In genomic DNA samples,the limit of detection of 0.1%can be achieved for EGFR-L858 R,demonstrating the potential of clinical application in our design. | Lida Ren Zhihao Ming Wei Zhang Yangwei Liao Xiaofeng Tang Bei Yan Huimin Lv Xianjin Xiao | 2022 | Chinese Chemical Letters2022，33，6： | 0 |
| 3 | The crystallographic texture and dependent mechanical properties of the CrCoNi medium-entropy alloy by laser remelting strategy显示文摘Laser remelting(LR)has attracted widespread attention in recent years as an effective method to reduce internal defects and improve the surface quality of additively manufactured(AM)parts.In the present study,three different LR inter-layer scanning strategies(LR0,LR90 and LR45)and their effects on the porosity,microstructure,crystallographic texture and related mechanical properties of parts have been studied.Optical microscope,X-ray diffraction,and scanning electron microscope were used as characterization tools.In the LR90 sample,it shows obvious{111}<110>texture and strong<111>preferred orientation along the scanning direction(SD),while the 0°offset and the 45°rotation of LR scanning strategy form a finer microstructure and weak crystallographic texture.Meanwhile,the mechanical properties of the LR sample are improved compared with the sample only by laser metal deposition(LMD),and a combination of higher strength and optimal uniform elongation is obtained in the LR45 sample.The overall results show that a reasonable LR scanning strategy can reduce the anisotropy of AM parts and improve their mechanical properties. | Pengsheng Xue Lida Zhu Jinsheng Ning Peihua Xu Shuhao Wang Zhichao Yang Yuan Ren Guiru Meng | 2022 | Journal of Materials Science & Technology2022，，16： | 0 |
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