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| 1 | Bax在普萘洛尔诱导人血管瘤内皮细胞凋亡中的作用显示文摘目的:通过不同浓度的普萘洛尔干预体外培养的血管瘤内皮细胞,探讨Bax基因在普萘洛尔诱导血管瘤内皮细胞凋亡中的作用。方法:体外培养人增殖期血管瘤内皮细胞,分别加入不同浓度的普萘洛尔药物干预,选择适宜的研究浓度及观察时间。构建Bax基因高表达及低表达组,检测其表达差异对于血管瘤内皮细胞凋亡的影响。结果:经普萘洛尔干预后细胞呈凋亡形态学改变,且随着药物浓度和药物作用时间的增加,细胞凋亡率也相应增加,其中300μmol/L的普萘洛尔干预24h时细胞凋亡率为28.49%,此为本实验中普萘洛尔诱导血管瘤内皮细胞凋亡的最佳药物浓度和时间点。Bax高表达组细胞构建成功后,经普萘洛尔干预,BAM7+普萘洛尔组>DMSO+普萘洛尔组>普萘洛尔组>BAM7组>空白对照组。Bax低表达组构建成功后,经普萘洛尔干预,BaxsiRNA+普萘洛尔组>N.CsiRNA+普萘洛尔组>转染试剂+普萘洛尔组>普萘洛尔组>空白对照组。结论:300μmol/L的普萘洛尔干预24h,是普萘洛尔诱导血管瘤内皮细胞凋亡的最佳药物浓度和时间点。Bax与普萘洛尔诱导血管瘤内皮细胞凋亡密切相关,Bax表达量越高,普萘洛尔诱导血管瘤内皮细胞凋亡的能力越强,Bax表达量降低,血管瘤内皮细胞凋亡无明显变化。 | 姚天华 Krishna Regmi 杜婧婷 史婧怡 虎小毅 李立峰 屠军波 | 2019 | 中国美容医学2019，28，1： | 2 |
| 2 | P53-MDM2在普萘洛尔诱导血管瘤内皮细胞凋亡中的作用显示文摘目的初步研究P53、MDM2与普萘洛尔诱导血管瘤内皮细胞凋亡的关系,为血管瘤的治疗提供新的思路和靶点。方法体外培养血管瘤内皮细胞,实验设计为2组,加药组和空白对照组,加药组以100umol/L的普萘洛尔处理细胞,空白对照组不予以特殊处理。倒置显微镜、电镜下观察形态学变化,流式细胞术检测细胞凋亡率,RTPCR、Western-blot检测MDM2、P53 mRNA水平和蛋白水平上的变化。结果用普萘洛尔处理后,大部分细胞形态有紧密排列的'铺路石'形态逐渐变圆。透射电镜观察到细胞呈现凋亡相,对照组无明显变化;加药组细胞凋亡率为14.67%,空白对照组4.87%,具有统计学差异。RT-PCR、Western-blot结果一致,与空白对照组相比,P53、MDM2均升高,具有统计学差异。结论 100umol/L普萘洛尔能够诱发体外培养的血管瘤内皮细胞凋亡,能促使体外培养的血管瘤内皮细胞中P53与MDM2呈现高表达。 | 姚天华 李全雁 李立峰 杜婧婷 Krishna Regmi 葛苏蒙 屠军波 | 2018 | 现代口腔医学杂志2018，32，1： | 1 |
| 3 | Foot-and-Mouth Disease Outbreak at Bageshwori Gaushala,Chitwan,Nepal显示文摘Cow being the national animal of Nepal has special place in the society.Religious institutions have since prehistoric time reared and cared for cattle herds and the tradition still continues.The Bageshwori Gaushala(cow shelter),initiated by the Hindu Rastraguru Yogi Narhaharinath in mid-1990s is one of the largest of such nucleus indigenous cattle herds in Nepal.Indigenous cattle conservation strategy of Nepal has recognized the importance of this nucleus herd,kept by grazing in the surrounding forest.Ethnoveterinary approaches of managing the herd health is in practice and vaccination was never done.Occasional outbreak did happen in past but overall the herd never suffered severe impact.In April 2018,an outbreak of foot-and-mouth disease(FMD)occurred at Bageshwori,Gaushala,Devghat,Bharatpur Metropolitan-1,Chitwan district of Nepal.In the bovine herd studied,80(53.33%)out of 150 affected animals presented characteristics FMD symptoms as vesicular lesions,drooling salivation,foot lameness,high fever and weakness.Following prompt supportive treatment,the condition improved.The complete recovery of infected herd was observed after 45 d.Laboratory analysis showed the presence of Aphthovirus serotype O in the clinical sample.The present study attempts to provide fresh insights into the recent FMD outbreak,clinical signs,their effective treatment protocol and preventive measures that must be practiced following FMD outbreak. | Milan Kandel Sachin Regmi Bablu Thakur Ramchandra Acharya Krishna Kaphle | 2018 | Journal of Agricultural Science and Technology(A)2018，8，6： | 0 |
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