|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Current status of diagnosis and treatment of bladder cancer in China-Analyses of Chinese Bladder Cancer Consortium database显示文摘Objective:To investigate current status of diagnosis and treatment of bladder cancer in China.Methods:A database was generated by Chinese Bladder Cancer Consortium(CBCC).From January 2007 to December 2012,14,260 cases from 44 CBCC centers were included.Data of diagnosis,treatment and pathology were collected.Results:The average age was 63.5 year-old and most patients were male(84.3%).The most common histologic types were urothelial carcinoma(91.4%),adenocarcinoma(1.8%),and squamous carcinoma(1.9%).According to 1973 and 2004 WHO grading system,42.0%,41.0%,and 17.0% of patients were grade 1,2,and 3,and 16.0%,48.7%,and 35.3% of patients were papillary urothelial neoplasms of low malignant potential,low,and high grade,respectively.Non-muscle invasive bladder cancer(NMIBC)and muscle invasive bladder cancer(MIBC)were 25.2% and 74.1%,respectively(0.8% not clear).Carcinoma in situ was only 2.4%.Most patients were diagnosed by white-light cystoscopy with biopsy(74.3%).Fluorescence and narrow band imaging cystoscopy had additional detection rate of 1.0% and 4.0%,respectively.Diagnostic transurethral resection(TUR)provided detection rate of 16.9%.Most NMIBCs were treated with TUR(89.2%).After initial TUR,2.6%accepted second TUR,and 45.7%,69.9%,and 58.7% accepted immediate,induced,and maintenance chemotherapy instillation,respectively.Most MIBCs were treated with radical cystectomy(RC,59.7%).Laparoscopic RCs were 35.1%,while open RC 63.4%.Extended and standard pelvic lymph node dissection were 7% and 66%,respectively.Three most common urinary diversions were orthotopic neobladder(44%),ileal conduit(31%),and ureterocutaneostomy(23%).Only 2.3% of patients accepted neo-adjuvant chemotherapy and only 18%of T3 and T4 patients accepted adjuvant chemotherapy.Conclusion:Disease characteristics are similar to international reports,while differences of diagnosis and treatment exist.This study can provide evidences for revisions of the guideline on bladder cancer in China. | Kaiwen Li Tianxin Lin 无 Wei Xue Xin Mu Enci Xu Xu Yang Fubao Chen Guangyong Li Lulin Ma Guoliang Wang Chaozhao Liang Haoqiang Shi Ming Li Mao Tang Xueyi Xue Yisong Lv Yaoliang Deng Chengyang Li Zhiwen Chen Xiaozhou Zhou Fengshuo Jin Xudong Liu Jinxin Wei Lei Shi Xin Gou Weiyang He Liqun Zhou Lin Cai Baiye Jin Guanghou Fu Xiangbo Kong Hongyan Sun Ye Tian Lang Feng Tiejun Pan Yiyi Wu Dongwen Wang Hailong Hao Benkang Shi Yaofeng Zhu Qiang Wei Ping Han Changli Wu Dawei Tian Zhangqun Ye Zheng Liu Zhiping Wang Junqiang Tian Lin Qi Minfeng Chen Wei Li Jinchun Qi Gongxian Wang Longlong Fu Zhaolin Sun Guangheng Luo Zhoujun Shen Zhaowei Zhu Jinchun Xing Zhun Wu Dong Wei Xin Chen Yanqun Na Hongfeng Guo Chunxi Wang Zhihua Lu Chuize Kong Yang Liu Jin Yang Jianyun Hu Xin Gao Jielin Li Changjun Yin Pu Li Shan Chen Zhen Du Jiongming Li Yongji Yan Xu Zhang Shuang Huang Fangjian Zhou Zhiling Zhang Yinghao Sun Shuxiong Zeng Song Cen Jiaquan Zhou Hanzhong Li Jin Wen Jian Huang | 2015 | Asian Journal of Urology2015，2，2： | 21 |
| 2 | Numerical simulation based on two-directional freeze and thaw algorithm for thermal diffusion model显示文摘Freeze-thaw processes significantly modulate hydraulic and thermal characteristics of soil. The changes in the frost and thaw fronts(FTFs) affect the water and energy cycles between the land surface and the atmosphere. Thus, the frozen soil comprising permafrost and seasonally frozen soil has important effects on the land surface hydrology in cold regions. In this study, a two-directional freeze and thaw algorithm is incorporated into a thermal diffusion equation for simulating FTFs. A local adaptive variable-grid method is used to discretize the model. Sensitivity tests demonstrate that the method is stable and FTFs can be tracked continuously. The FTFs and soil temperature at the Qinghai-Tibet Plateau D66 site are simulated hourly from September 1, 1997 to September 22, 1998. The results show that the incorporated model performs much better in the soil temperature simulation than the original thermal diffusion equation,showing potential applications of the method in land-surface process modeling. | Junqiang GAO Zhenghui XIE Aiwen WANG Zhendong LUO | 2016 | Applied Mathematics and Mechanics(English Edition)2016，37，11： | 4 |
| 3 | Construction of recombinant plasmid pEGFP-C2-L539fs/47 and its expression in HEK293 cells显示文摘Objective:To reconstruct pEGFP-C2-L539fs/47,a HERG nonsense mutant in eukaryotic expression plasmid,and observe the fusion protein expressed in HEK293 cells(human embryo kidney cells).Methods:After double digestion of pcDNA3-L539fs/47 and pEGFP-C2-HERG with sbf I and Eco91 I,the small product fragment,from pcDNA3-L539fs/47,was subcloned into the big fragment of pEGFP-C2-HERG under T4 ligase.pEGFP-C2-L539fs/47 was identified by agarose gel electrophoresis and sequencing.pcDNA3-L539fs/47 and pEGFP-C2-L539fs/47 were transiently transfected into HEK293 cells by Lipofect,respectively.The expression of fusion protein in HEK293 cells was detected through immunofluorescence,laser confocal imaging scanning in vivo,Western blot and PCR.Results:Mutation region cDNA fragment(about 1 kb) and target vector fragment(about 7.2 kb) were ligated after purification and gel recovery.Agarose gel electrophoresis and sequencing successfully demonstrated eukaryotic expression plasmid pEGFP-C2-L539fs/47,constructed approximately 8.2 kb,sequencing consistent with template gene.The transfection efficiency of recombinant plasmid by fluorescence microscopy was more than60%.Western blot analysis detected pcDNA3-L539fs/47 expression of the protein size 60 KD,the expression of pEGFP-C2 fusion protein size of approximately 90 KD.The L539fs/47 gene expression in HEK293 cells was significant by PCR analysis.Confocal laser imaging showed that pEGFP-C2-L539fs/47 protein was successfully expressed in cytoplasm and cytomembrane of HEK293 cells.Conclusion:pEGFP-C2-L539fs/47 containing the HERG gene mutant was successfully constructed by double digestion method and expressed fusion protein in HEK293 cells,which laid a foundation for the further study on L539fs/47. | Lue Ying Zhang Aifeng Han Wenqi Li Guoliang Zhang Junbo Gao Jie Pan Junqiang Zhang Yong Sun Chaofeng | 2012 | Journal of Medical Colleges of PLA(China)2012，27，3： | 2 |
| 4 | Design and fabrication of compact Ge-on-SOI coupling structure显示文摘In this paper, we have proposed and demonstrated a simple approach to fabricate vertical integrated structure for coupling between active germanium (Ge) waveguide and silicon-on-insulator (SOI) waveguide. The active Ge waveguide is sputtered after etching the underlying passive silicon (Si) waveguide. This method scuttles away from the difficulty involved in the waveguide fabrication by avoiding the etching process for the Ge waveguide, and thereby the waveguide quality is improved. The influences of the coupling structural parameters on the coupling loss are analyzed and discussed. The optimizing parameters are obtained for the fabrication. The minimal coupling loss is experimentally measured about 2.37 dB, and variation tendency of coupling loss against the structural parameters is consistent with the theoretical result. The proposed approach offers an effective path for vertical coupling between Ge and SOI optical components. | Jianfeng GAO Junqiang SUN Heng ZHOU Jialin JIANG Yang ZHOU | 2019 | Frontiers of Optoelectronics2019，12，3： | 0 |
| 5 | MicroRNAs in tumor stem cells显示文摘Micro RNAs(mi RNAs) are a class of non-coding RNAs that are believed to have a significant role in tumorigenesis and cancer metastasis. Cancer stem cells play a major role in tumor recurrence, metastasis, and drug resistance. Research has shown that mi RNAs can promote or inhibit the stemness of cancer stem cells and regulate the differentiation and self-renewal of cancer stem cells. In this article, the phenotype and regulatory mechanisms of mi RNAs in cancer stem cells will be described, together with an explanation of their potential role in tumor diagnosis and treatment. | Xiaochen Hu Junqiang Yang Ruijie Yang Ruina Yang Xinshuai Wang Shegan Gao | 2015 | The Chinese-German Journal of Clinical Oncology2015，14，2： | 0 |
| 6 | Construction of a HERG mutant L539fs/47-*558W pEGFP vector and the expression of the fusion protein in HEK293 cells显示文摘Objective:To construct a human ether-a-go-go-related gene(HERG)nonsense mutant L539fs/47-*558W into the autonomously fluorescent,eukaryotic expression vector pEGFP-C2,and to verify expression of the reconstruct in human embryonic kidney-293(HEK293)cells.Methods:The mutational fragment was subcloned into pEGFP-C2-HERG by double digestion of SbfⅠ,Eco91Ⅰand rejoining of T4 ligase.After verification,the recombinant pEGFP-C2-L539fs/47-*558W and pEGFP-C2-HERG were respectively transfected into HEK293 cells for 48 h by the Lipofect method to observe the expression location of the fusion protein by laser confocal imaging scanning in vivo.pcDNA3-L539fs/47-*558W and pcDNA3-HERG were transfected to observe the expression location of the HERG protein by immunofluoresceoce.The mutant protein size was determined by Western blotting.Results:The about 1 kb-sized mutation region cDNA fragment from pcDNA3-L539fs/47-*558W and the about 7.2 kb-sized target vector fragment from pcDNA3-HERG were ligated after purification and gel recovery.pEGFP-C2-L539fs/47*-558W,approximately 8.2 kb,was demonstrated successfully been constructed under agarose gel electrophoresis and further sequencing.Laser confocal imaging showed that pEGFP-C2-HERG was mainly expressed in the membrane,whereas truncated mutant-type HERG in the pEGFP-C2 vector was partially located in the cytoplasm,the others were transported to the cell membrane in living HEK293 cells.The same as the immunofluoresceoce results after transfection of pcDNA3-HERG and pcDNA3-L539fs/47-558W.Wild-type HERG-GFP fusion protein expressed 160 and 180 kDa bands.The mutant and mutant-GFP fusion proteins were 70 and 100 kDa,respectively.Conclusion:pEGFP-C2-L539fs/47-*558W was successfully constructed by double digestion method GFP had no effect on its protein expression and trafficking in HEK293 cells,which laid a foundation for the further study on L539fs/47-*558W. | ZHANG Junbo Lü Ying ZHANG Aifeng SUN Chaofeng HAN Wenqi LI Guoliang GAO Jie HUO Jianhua PAN Junqiang ZHOU Xin NIU Xiaolin | 2013 | Journal of Medical Colleges of PLA(China)2013，28，4： | 0 |
| 7 | 乙肝病毒相关肝细胞癌的整合蛋白基因组学特征性研究显示文摘文章简介研究团队利用159例患者手术切除的肿瘤组织和临近的正常组织,首次进行了乙肝病毒相关肝细胞癌的蛋白基因组学特征性研究。通过蛋白基因组学整合分析,我们揭示了乙肝病毒相关肝细胞癌在多组学,关键信号通路激活状态,以及肝脏特异性代谢重编程等方面的诸多一致性和不一致性。 | Qing Gao Hongwen Zhu Liangqing Dong Weiwei Shi Ran Chen Zhijian Song Chen Huang Junqiang Li Xiaowei Dong Yanting Zhou Qian Liu Lijie Ma Xiaoying Wang Jian Zhou Yansheng Liu 8 Emily Boja Ana I.Robles Weiping Ma Pei Wang Yize Li Li Ding Bo Wen Bing Zhang Henry Rodriguez 高大明 周虎 樊嘉 | 2020 | 科学新闻2020，，2： | 0 |
| 8 | Stability of selenium and its speciation analysis in water using automatic system separation and HR-ICP-MS measurement显示文摘A simple and convenient method has been developed for the pre-concentration and separation of inorganic selenium species from environmental water samples using anion exchange chromatographic column combined with high resolution inductively coupled plasma mass spectrometry(HR-ICP-MS)measurement.^(75)Se(Ⅳ) and ^(75)Se(Ⅵ)were prepared and used as tracers during the experiments.The volatility of selenium during solution evaporation was investigated to establish a reliable water samples pretreatment procedure.The parameters which affect the uptake of Se(Ⅳ)and Se(Ⅵ)on Dowex1×8 resin was optimized and the procedure for Se(Ⅳ)and Se(Ⅵ)separation was proposed.Both Se(Ⅳ)and Se(Ⅵ)are retained on the column in natural or alkaline solution with high distribution coefficient.The successive gradient elution of pre-concentrated species of selenium with HNO_(3)solution allows to differentiate between them.Se(Ⅳ)and Se(Ⅵ)finally were eluted with 0.05 mol/L HNO_(3) and 5.0 mol/L HNO_(3),respectively.The proposed method has been successfully verified using the certified reference materials(CRMs)of real water samples,and spiked recoveries for real samples were 98%-104%with 5%relative standard deviations(RSDs).The developed procedure is proved to be reliable and can be used for the rapid determination of selenium species in environmental water samples. | Junqiang Yang Yawen Chen Keliang Shi Kesheng Hu Run Li Xiaoqing Gao Qian Wang Weibo Zhang Yun Zhou Yanyun Wang Jiangang He Tonghuan Liu Xiaolin Hou | 2022 | Chinese Chemical Letters2022，33，7： | 0 |
| 9 | 模拟多年冻土活动层厚度对气候变化的响应显示文摘在气候变化背景下,活动层厚度的变化会对多年冻土区水文,生态,寒区工程等产生较大的影响.本研究利用中科院气候系统模式CAS-FGOALS-g3和陆面过程模式CAS-LSM模拟分析了活动层厚度的变化趋势和相对变化.结果表明:活动层厚度整体上呈现出增加的趋势.1979-2014年,多年冻土区活动层厚度的区域平均为1.08 m,变化趋势为0.33 cm yr-1,其变化趋势与2 m气温变化趋势基本一致,相对变化范围为1%-58%,平均为10.9%.在未来四种不同的气候情景(SSP-2.6,SSP2-4.5,SSP3-7.0和SSP5-8.5)下,到2100年预计活动层厚度的相对变化分别为10.3%,14.6%,30.1%和51%. | Ruichao Li Jinbo Xie Zhenghui Xie Junqiang Gao Binghao Jia Peihua Qin Longhuan Wang Yan Wang Bin Liu Si Chen | 2021 | Atmospheric and Oceanic Science Letters2021，14，1： | 0 |
| 10 | Identification of potential key genes in resveratrol biosynthesis via transcriptional analyses of berry development in grapevine(Vitis spp.)genotypes varying in trans-resveratrol content显示文摘Resveratrol is an important secondary metabolite not only owing to its function as a phytoalexin,but also its potential benefits to human health.In this study,the content of trans-resveratrol was documented in seven accessions of grapevine,in the seed,pulp and skin of berries,and at three developmental stages.The highest amount(2.99μg g^(−1) FW)was found in the skin of berries at the ripe stage from V.amurensis‘Tonghua-3'.Resveratrol was not detected in several samples,including skin of berries at the green hard or véraison stage from V.davidii‘Tangwei'.We carried out transcriptional profiling of developing‘Tonghua-3'and‘Tangwei'berries to identify gene expression patterns that may be linked with the difference in resveratrol content between these accessions.The expression levels of several differentially expressed genes(DEGs)with presumed function in resveratrol biosynthesis,including STILBENE SYNTHASEs(STSs),CINNAMATE 4-HYDROXYLASEs(C4Hs)and 4-COUMARATE-COA LIGASEs(4CLs),were significantly higher in‘Tonghua-3',than in'Tangwei'during the véraison and ripe stages.Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses suggested that these DEGs were enriched for multiple biological processes at the three stages of fruit development.Additionally,we identified a total of 36 transcription factors,including MYBs,WRKYs,ERFs,bHLHs and bZIPs,that were coexpressed with 17 STSs via a weighted gene co-expression network analysis,suggesting roles as regulators of resveratrol biosynthesis.Overall,these findings provide insight into genotypic differences in resveratrol biosynthesis in grapevine,as well as the molecular genetics of its regulation. | Xiuming Zhang Yifei Wu Yichu Zhang Xiangjing Yin Steve van Nocker Junqiang Guo Zhi Li Min Gao Junyang Song Xiping Wang | 2022 | Fruit Research2022，2，1： | 0 |
| 11 | Calculation Model for the Steady-State Vibration Amplitude of a New Type of Cascaded Composite Structure-Based Ultrasonic Transducer显示文摘The steady-state vibration amplitude is an important performance indicator of high-frequency ultrasonic transducers for ultrasonically assisted manipulating,machining,and manufacturing.This work aimed to develop a calculation model for the steady-state vibration amplitude of a new type of dual-branch cascaded composite structure-based ultrasonic transducer that can be used in the packaging of microelectronic chips.First,the steady-state vibration amplitude of the piezoelectric vibrator of the transducer was derived from the piezoelectric equation.Second,the vibration transfer matrices of the tapered ultrasonic horns were obtained by combining the vibration equation,the continuous condition of the displacement,and the equilibrium condition of the force.Calculation models for the steady-state vibration amplitude of the two working ends of the transducer were then developed.A series of exciting trials were carried out to test the performance of the models.Comparison between the calculated and measured results for steady-state vibration amplitude showed that the maximum deviation was 0.0221μm,the minimum deviation was 0.0013μm,the average deviation was 0.0097μm,and the standard deviation was 0.0046μm.These values indicated good calculation accuracy,laying a good foundation for the practical application of the proposed transducer. | Hongjie Zhang Xinyue Gao Xiaochen Liu Junqiang Wu | 2023 | Nanomanufacturing and Metrology2023，6，3： | 0 |
| 12 | Coupling of the Calculated Freezing and Thawing Front Parameterization in the Earth System Model CAS-ESM显示文摘The soil freezing and thawing process affects soil physical properties,such as heat conductivity,heat capacity,and hydraulic conductivity in frozen ground regions,and further affects the processes of soil energy,hydrology,and carbon and nitrogen cycles.In this study,the calculation of freezing and thawing front parameterization was implemented into the earth system model of the Chinese Academy of Sciences(CAS-ESM)and its land component,the Common Land Model(CoLM),to investigate the dynamic change of freezing and thawing fronts and their effects.Our results showed that the developed models could reproduce the soil freezing and thawing process and the dynamic change of freezing and thawing fronts.The regionally averaged value of active layer thickness in the permafrost regions was 1.92 m,and the regionally averaged trend value was 0.35 cm yr–1.The regionally averaged value of maximum freezing depth in the seasonally frozen ground regions was 2.15 m,and the regionally averaged trend value was–0.48 cm yr–1.The active layer thickness increased while the maximum freezing depth decreased year by year.These results contribute to a better understanding of the freezing and thawing cycle process. | Ruichao LI Jinbo XIE Zhenghui XIE Binghao JIA Junqiang GAO Peihua QIN Longhuan WANG Si CHEN | 2023 | Advances in Atmospheric Sciences2023，40，9： | 0 |
| 13 | Doctors' faith显示文摘We make some efforts to find some connections between religion and medical science. Medical science can be regarded as sacred and holy as a religion for doctors to believe. The common points between them are the basis to successfully integrate them into doctors' faith. Furthermore, we explain that doctors need a faith to believe so as to meet the demand of humanitarian traits we should have and to do better in the scientific work and win in the battles with the diseases. | ZHANG Junbo Lü Ying ZHANG Aifeng SUN Chaofeng HAN Wenqi LI Guoliang GAO Jie HUO Jianhua PAN Junqiang ZHOU Xin NIU Xiaolin | 2013 | Journal of Medical Colleges of PLA(China)2013，28，1： | 0 |
| 14 | Differentiation between two healthcare concepts:Person-centered and patient-centered care显示文摘1.Background Engel's biopsychosocial model,which was constructed in 1977,was considered a momentous breakthrough from the traditional biomedical model of medicine that regards the person as a disease-carrier requiring diagnosis and treatment[1]towards recognition of the whole person and furnish customized care.This model argues that health should not be considered solely on biological factors but on a combination of biological,psychological,and social factors[2].This idea of considering individuals as a whole and respecting their rights to self-determination has led to a shift in the care model from disease-centered care to patient-centered or person-centered care.The latter two care models are both abbreviated as PCC.Ensuring that people are involved in and central to the healthcare process is now recognized as a key component in developing high-quality care.Studies have revealed that both concepts of care can help improve people's health condition and lower health service burdens.Thus,countries and healthcare organizations have been paying close attention to the patients'concerns.The National Health Service in England has made person-centered care as one of its seven core principles[3]. | Junqiang Zhao Shangqian Gao Jinfang Wang Xinjuan Liu Yufang Hao | 2016 | International Journal of Nursing Sciences2016，3，4： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员