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| 1 | Development and optimization of thermal contrast amplification lateral flow immunoassays for ultrasensitive HIV p24 protein detection显示文摘Detection of human immunodeficiency virus(HIV)p24 protein at a single pg/ml concentration in point-of-care(POC)settings is important because it can facilitate acute HIV infection diagnosis with a detection sensitivity approaching that of laboratory-based assays.However,the limit of detection(LOD)of lateral flow immunoassays(LFAs),the most prominent POC diagnostic platform,falls short of that of laboratory protein detection methods such as enzyme-linked immunosorbent assay(ELISA).Here,we report the development and optimization of a thermal contrast amplification(TCA)LFA that will allow ultrasensitive detection of 8 pg/ml p24 protein spiked into human serum at POC,approaching the LOD of a laboratory test.To achieve this aim,we pursued several innovations as follows:(a)defining a new quantitative figure of merit for LFA design based on the specific to nonspecific binding ratio(BR);(b)using different sizes and shapes of gold nanoparticles(GNPs)in the systematic optimization of TCA LFA designs;and(c)exploring new laser wavelengths and power regimes for TCA LFA designs.First,we optimized the blocking buffer for the membrane and running buffer by quantitatively measuring the BR using a TCA reader.The TCA reader interprets the thermal signal(i.e.,temperature)of GNPs within the membrane when irradiated by a laser at the plasmon resonance wavelength of the particle.This process results in higher detection and quantitation of GNPs than in traditional visual detection(i.e.,color intensity).Further,we investigated the effect of laser power(30,100,200 mW),GNP size and shape(30 and 100 nm gold spheres,150 nm gold-silica shells),and laser wavelength(532,800 nm).Applying these innovations to a new TCA LFA design,we demonstrated that 100nm spheres with a 100 mW 532 nm laser provided the best performance(i.e.,LOD=8pg/ml).This LOD is significantly better than that of the current colorimetric LFA and is in the range of the laboratory-based p24 ELISA.In summary,this TCA LFA for p24 protein shows promise for detecting acute HIV infection in POC settings. | Li Zhan Timothy Granade Yilin Liu Xierong Wei Ae Youngpairoj Vickie Sullivan Jeff Johnson John Bischof | 2020 | Microsystems & Nanoengineering2020，6，1： | 3 |
| 2 | Boardsof directors:a review and research agenda显示文摘 | JOHNSON JL DAILY CM ELLSTRAND AE | 1996 | Journal of Management1996，22，3： | 1 |
| 3 | Clinical experience of lymphangioleiomyomatosis in the UK显示文摘 | Johnson SR Tattersfield AE | 2000 | Thorax2000，55，： | 1 |
| 4 | Decline in lung function in lymphangioleiomyomatosis: relation to menopause and progesterone treatment显示文摘 | Johnson SR Tattersfield AE | 1999 | Am J Respir Crit Care Med1999，160，： | 1 |
| 5 | CDC42 And CDC43 ,two additional genes involved in budding and the estab- lishment of cell polarity in the yeast Saccharomyces cerevisiae显示文摘 | Alison AE Johnson DI Longneeker RM | 1990 | J Cell Bio11990，111，1： | 1 |
| 6 | Analysis of the PINK1 gene in a large cohort of cases with Parkinson disease显示文摘 | Johnson J Lang AE | 2004 | Arch Neuro12004，61，： | 1 |
| 7 | Accelerated thrombolysis and reperfusion in a canine model of myocardial infarction by liposomal encapsulation of strptokinase显示文摘 | NGUYEN PD OREAR EA JOHNSON AE | 1990 | Circ Res1990，66，： | 1 |
| 8 | Occupational asthma in greenhouse tomato growing显示文摘 | Erlam AE Johnson AJ Wiley KN | 1996 | Occup Med1996，46，2： | 1 |
| 9 | Intraventricular penicillin:a note of warning显示文摘 | Johnson HC Walker AE | 1945 | JAMA1945，27，4： | 1 |
| 10 | Guidelines for classification and interpretation of group blood and urine data collected as part of the Na- tional Nutrition Survey显示文摘 | O' Neal RM Johnson OC Schaefer AE | 1970 | Pediatr Res1970，4，1： | 1 |
| 11 | Occupational asthma in greenhouse tomato growing 显示文摘 | FZrlam AE Johnson AJ Wiley KN | 1996 | Occup Med1996，46，2： | 1 |
| 12 | Clinical experience of lymphangioleiomyomatosis in the UK显示文摘 | Johnson SR Tattersfield AE | 2000 | Thorax2000，55，： | 1 |
| 13 | Use of the posteriorly based lateral tongue flap in the repair of palatal fistulae 显示文摘 | Johnson PA Banks P Brown AE | 1992 | Int J Oral Maxillofac Surg1992，21，： | 1 |
| 14 | Measuring polymerization shrinkage of photo activatel restorative materials by a water filled显示文摘 | LaiJH Johnson AE | 1993 | Dent Matr1993，9，： | 1 |
| 15 | Analysis of the PINK1 gene in a large cohort of cases with Parkinson disease显示文摘 | Rogaeva E Johnson J Lang AE | | 0，，12： | 1 |
| 16 | Maternal feeding practices and beliefs and their relationships to overweight in early childhood显示文摘 | Baughcum AE Powers SW Johnson SB | 2001 | J Dev Behav Pediatr2001，22，6： | 1 |
| 17 | Nutrition as- sessment and management in advanced liver disease 显示文摘 | Johnson TM Overgard EB Cohen AE | 2013 | Nutr Clin Pract2013，28，1： | 1 |
| 18 | Anode biofilm transcriptomics reveals outer surface components essential for high density current production in Geobacter sulfurreducens fuel cells 显示文摘 | Nevin KP Kim BC Glaven RH Johnson JP Woodard TL Meth6 BA DiDonato Jr RJ Covalla SF Franks AE Liu A | 2009 | PLoS One2009，4，5： | 1 |
| 19 | Obstetric complica- tions of ureteroseopy during pregnancy 显示文摘 | Johnson EB Krambeek AE White WM | 2012 | Urol2012，188，1： | 1 |
| 20 | Decline in lung function in lymphangioleiomyornatosis , relation to menopause and progesterone treatment 显示文摘 | Johnson SR Tattersfield AE | 1999 | Am J Respir Crit Care Med1999，160，： | 1 |
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