维普期刊资源整合服务平台（V6.5）

维普中文期刊产品整合服务

4篇 您的检索式：作者名="Jochen Ringe"

选中清除导出查看参考文献查看引证文献引用追踪共1页首页上一页第1页下一页末页 /1 跳转

		题名	作者	年代	出处	被引量
	1	Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic me- dium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reac- tion (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo.	ZHENG Yi-xiong RINGE Jochen LIANG Zhong LOCH Alexander CHEN Li SITTINGER Michael	2006	Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，10：	8
	2	Chitosan‐based injectable hydrogel as a promising in situ forming scaffold for cartilage tissue engineering显示文摘	Hojjat Naderi‐Meshkin Kristin Andreas Maryam M. Matin Michael Sittinger Hamid Reza Bidkhori Naghmeh Ahmadiankia Ahmad Reza Bahrami Jochen Ringe	2014	Cell Biol Int2014，，1：	1
	3	Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘	Yi-xiong Zheng Jochen Ringe Zhong Liang Alexander Loch Li Chen Michael Sittinger	2006	Journal of Zhejiang University SCIENCE B2006，，10：	1
	4	Emulsion-based synthesis of PLGA-microspheres for the in vitro expansion of porcine chondrocytes显示文摘	Franziska Gabler Simone Frauenschun Jochen Ringe	2007	Biomolecular Engineering2007，，05：	1

返回顶部每页显示：

共1页首页上一页第1页下一页末页 /1 跳转

goolge学术搜索-维普

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费