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| 1 | CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway显示文摘With its high efficiency for site-specific genome editing and easy manipulation,the clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR associated protein 9(CAS9)system has become the most widely used gene editing technology in biomedical research.In addition,significant progress has been made for the clinical development of CRISPR/CAS9 based gene therapies of human diseases,several of which are entering clinical trials.Here we report that CAS9 protein can function as a genome mutator independent of any exogenous guide RNA(gRNA)in human cells,promoting genomic DNA double-stranded break(DSB)damage and genomic instability.CAS9 interacts with the KU86 subunit of the DNA-dependent protein kinase(DNA-PK)complex and disrupts the interaction between KU86 and its kinase subunit,leading to defective DNA-PK-dependent repair of DNA DSB damage via non-homologous end-joining(NHEJ)pathway.XCAS9 is a CAS9 variant with potentially higher fidelity and broader compatibility,and dCAS9 is a CAS9 variant without nuclease activity.We show that XCAS9 and dCAS9 also interact with KU86 and disrupt DNA DSB repair.Considering the critical roles of DNA-PK in maintaining genomic stability and the pleiotropic impact of DNA DSB damage responses on cellular proliferation and survival,our findings caution the interpretation of data involving CRISPR/CAS9-based gene editing and raise serious safety concerns of CRISPR/CAS9 system in clinical application. | Shuxiang Xu Jinchul Kim Qingshuang Tang Qu Chen Jingfeng Liu Yang Xu Xuemei Fu | 2020 | Protein & Cell2020，11，5： | 8 |
| 2 | An experimental study on the durability of high performance shotcrete for permanent tunnel support显示文摘 | Sangpil Lee Donghyun Kim Jonghyun Ryu Sangdon Lee Jinchul Kim Hongsam Kim Myungsik Choi | 2006 | Tunnelling and Underground Space Technology incorporating Trenchless Technology Research2006，，3： | 1 |
| 3 | Isolation and characterization of diazotrophic growth promoting bacteria from rhizosphere of agricultural crops of Korea显示文摘 | Myoungsu Park Chungwoo Kim Jinchul Yang Hyoungseok Lee Wansik Shin Seunghwan Kim Tongmin Sa | 2004 | Microbiological Research2004，，2： | 1 |
| 4 | RNA helicase MTR4 drives tumorigenesis of nasopharyngeal carcinoma by regulating the expression of key cell cycle genes显示文摘Dear Editor,The pathogenesis of nasopharyngeal carcinoma(NPC)is closely associated with the infection of Epstein Barr virus(EBV)(Hau et al.,2020).However,the mechanisms underlying NPC tumorigenesis remain unclear,leading to a lack of therapeutic targets to effectively treat NPC.RNA helicase MTR4,which is a compo-nent of the virus-induced cofactor complex linking viral RNA to exosome for degradation,is overexpressed in NPCs of patients.The expression levels of MTR4 are inversely correlated with the prognosis of NPC patients. | Lili Yu Lei Jiang Meng Wu Wenlong Dou Kaiyuan Ji Jianlong Zhou Jinchul Kim Yang Xu | 2023 | Protein & Cell2023，14，2： | 0 |
| 5 | 野生型p53通过激活PUMA抑制氧化磷酸化而促进肿瘤代谢显示文摘文章简介在一半的人类癌症中,肿瘤抑制因子p53发生了基因突变。然而令人困惑的是,在某些人类癌症如肝癌(HCC)中常常保留有野生型p53。本研究最新研究发现,野生型p53可通过诱导PUMA来抑制丙酮酸驱动的氧化磷酸化,从而产生致癌作用。IκB激酶介导PUMA在位点Ser96/106磷酸化,与线粒体丙酮酸载体(MPC)发生相互作用,进而破坏MPC寡聚化和功能,以抑制线粒体丙酮酸的摄取。 | Jinchul Kim Lili Yu Wancheng Chen Yanxia Xu Meng Wu Dilyana Todorova Qingshuang Tang Bingbing Feng Lei Jiang Jingjin He Guihua Chen 付雪梅 Yang Xu | 2020 | 科学新闻2020，，2： | 0 |
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