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| 1 | Apolipoprotein E, angiotensin-converting enzyme and kallikrein gene polymorphisms and the risk of Alzheimer's disease and vascular dementia显示文摘 | Wang HK Fung HC Hsu WC Wu YR Lin JC Ro LS Chang KH Hwu FJ Hsu Y Huang SY Lee-Chen GJ Chen CM | 2006 | 中国生物学文摘2006，20，8： | 8 |
| 2 | Novel functional proteins interact with midkine in hepatic cancer cells显示文摘BACKGROUND: Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. Patients with high tumor midkine expression frequently have a worse prognosis than those with low expression. The present study was designed to investigate the interaction network of midkine in hepatic cancer cells, and to elucidate its role in hepatocellular carcinoma. METHODS: DNA encoding full-length midkine was cloned into pDBLeu vector to serve as bait in yeast two-hybrid screening to identify interacting proteins. Candidate proteins were examined on SC-Leu-Trp-His+3-AT (20 mmol/L) plates and assayed for X-gal activity, then sequenced and classified according to the GenBank. Finally, identified proteins were expressed by the in vitro expression system pCMVTnT, and protein interactions were confirmed by co-immunoprecipitation. RESULTS: Using the yeast two-hybrid system, we found 6 proteins that interacted with midkine: NK-kappa-B inhibitor alpha (I-κ-B-α), Dvl-binding protein naked cuticle 2, granulin, latent active TGF-β binding protein 3, latent active TGF-β binding protein 4, and phospholipid scramblase 1. In vitro co-immunoprecipitation demonstrated that all identified proteins directly interacted with midkine.CONCLUSION: The identification of midkine-interacting proteins in hepatic cancer cells indicates that midkine is a multifunctional factor that may participate in cell migration, differentiation, and proliferation, and is also associated with the multicellular response feedback during the development of hepatocellular carcinoma. | Qiang Yan, Hui-Lian Huang, Xing Yao, Jing Li, Li-Qin Li, Jing Zhong, Li-Shan Min, Li-Cheng Dai and Shu-Sen Zheng Department of Hepatobiliary and Pancreatic Surgery, Huzhou Central Hospital (Yan Q and Yao X) Huzhou Key Laboratory of Molecular Medicine, Affiliated Central Hospital of Huzhou Teachers College (Huang HL, Li J, Li LQ, Zhong J, Min LS and Dai LC), Huzhou 313000, China Division of Hepatobiliary and Pancreatic Surgery, First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China (Yan Q and Zheng SS) | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，3： | 7 |
| 3 | Influence of lead acetate on soil microbial biomass and community structure in two different soils with the growth of Chinese cabbage (Brassica chinensis)显示文摘 | Liao M Chen CL Zeng LS Huang CY | 2007 | 中国生物学文摘2007，21，7： | 5 |
| 4 | Partitioning regularity of nonionic organic mixtures in organic phase/water system显示文摘The partitioning regularity of nonionic organic mixtures in organic phase/water system is revealed. The equation for calculating the partition coefficients of mixtures (KMD), together with the determination model, is derived from the equilibrium partitioning models (EPMs). Based on these derived equations, the KMD values of 20 mixtures containing halogenated benzenes are obtained. The results show that stronger hydrophobidty of an individual chemical in the mixture results in the stronger hydrophobidty of the mixture and the greater the proportion of this chemical, the stronger the hydrophobidty of the mixture will be. This partitioning regularity is helpful to the study of the toxicity for mixtures and the environmental behavior, such as transfer or accumulation, for mixed organic pollutants. | Lin, ZF Yu, HX Huang, LM Feng, JF Wang, LS | 2001 | Chinese Science Bulletin2001，46，17： | 2 |
| 5 | High hepatitis B viral load predicts recurrence of small hepatocellular carcinoma after curative resection显示文摘 | Qu LS Jin F Huang XW | 2010 | J Gastrointest Surg2010，14，7： | 1 |
| 6 | Attenuation of oxidative stress after varicocelectomy in subfertile patients with varicocele 显示文摘 | Chen SS Huang WJ Chang LS | 2008 | J Urol2008，179，2： | 1 |
| 7 | miR-205 targets PTEN and PHLPP2 to augment ANT signaling and drive malig- nant phenotypes in Non-Small cell lung caneer显示文摘 | Cai JC Fatg LS Huang YB | 2013 | Cancer Res2013，73，17： | 1 |
| 8 | Antigenic diversity of Neisse- ria meningitidis isolated in Taiwan between 1995 and 2002 显示文摘 | Yang CY Lee YS Huang LS | 2006 | Scand J Infect Dis2006，38，4： | 1 |
| 9 | Impairment of cardiac insulin signaling and myocardial contractile performance in highcholesterol/fructose-fed rats显示文摘 | DENG JY HUANG JP LU LS et ol | 2007 | Am J Physiol Heart Circ Physiol2007，293，2： | 1 |
| 10 | Truncation by Glu180 nonsense mutation results in complete loss of slow skeletal muscle troponin T in a lethal nemaline myopathy显示文摘 | Jin JP Brotto MA Hossain MM Huang QQ Brotto LS Nosek TM | 2003 | J Biol Chem2003，278，26： | 1 |
| 11 | Diabetes adversely affects macrophages during atherosclerotic plaque regression in miee显示文摘 | Parathath 5 Grauer L Huang LS | 2011 | Diabetes2011，60，6： | 1 |
| 12 | Production and characterization of bioactive recombinant resistin in Escherichia coli显示文摘 | Juan CC Kan LS Huang CC | 2003 | J Biotech- nol2003，103，2： | 1 |
| 13 | A clinical study of Hirayama disease in Taiwan显示文摘 | HUANG YC RO LS CHANG HS | | 0，，05： | 1 |
| 14 | Verification of linear range ofdeter-mination of platelet by XE2100显示文摘 | Huang FD Yan ZB Liao LS | | 0，，01： | 1 |
| 15 | Targeting sphingosine kinase1 attenuates bleomycin-induced pulmonary fibrosis显示文摘 | Huang LS Berdyshev E Mathew B Fu P Gorshkova IA He D | 2013 | FASEB J2013，27，4： | 1 |
| 16 | Interferon-alpha therapy after curative resection prevents early recurrence and improves surviv- al in patients with hepatitis B virus-related hepatocellular carci- noma显示文摘 | Qu LS Jin F Huang XW | 2010 | J Surg Oncol2010，102，7： | 1 |
| 17 | Conjugated linoleic acid in-duces apoptosis through estrogen receptor alpha in human breast tissue显示文摘 | Wang LS Huang Yw Liu SL | 2008 | BMC Cancer2008，8，： | 1 |
| 18 | Subharmonics and vortex merging in mixing layers显示文摘 | Ho CM Huang LS | | 0，，： | 1 |
| 19 | Visual field defects in patients with pituitary adenomas显示文摘 | Huang WC Lee LS | 1997 | Zhonghua Yi Xue Za Zhi (Taipei)1997，60，： | 1 |
| 20 | A simple, high sensitivity mutation screening using Ampligase mediated T7 endonuclease I and Surveyor nuclease with microfluidic capillary electrophoresis显示文摘 | Huang MC Cheong WC Lim LS | 2012 | Electrophoresis2012，33，5： | 1 |
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