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| 1 | The adult human testis transcriptional cell atlas显示文摘Human adult spermatogenesis balances spermatogonial stem cell (SSC) self-renewal and differentiation,alongside complex germ cell-niche interactions,to ensure long-term fertility and faithful genome propagation. Here,we performed single-cell RNA sequencing of -6500 testicular cells from young adults. We found five niche/somatic cell types (Leydig,myoid,Sertoli,endothelial,macrophage),and observed germline-niche interactions and key human-mouse differences. Spermatogenesis,including meiosis,was reconstructed computationally,revealing sequential coding,non-coding,and repeat-element transcriptional signatures. Interestingly,we identified five discrete transcriptional/developmental spermatogonial states,including a novel early SSC state,termed State 0. Epigenetic features and nascent transcription analyses suggested developmental plasticity within spermatogonial States. To understand the or丨gin of State 0,we profiled testicular cells from infants,and identified distinct similarities between adult State 0 and infant SSCs. Overall,our datasets describe key transcriptional and epigenetic signatures of the normal adult human testis,and provide new insights into germ cell developmental transitions and plasticity. | Jingtao Guo Edward J. Grow Hana Mlcochova Geoffrey J. Maher Cecilia Lindskog Xichen Nie Yixuan Guo Yodai Takei Jina Yun Long Cai Robin Kim Douglas T. Carrell Anne Goriely James M. Hotaling Bradley R. Cairns | 2018 | Cell Research2018，28，12： | 12 |
| 2 | A comparison of the relative efficiency of ICSI and extended culture with epididymal sperm versus testicular sperm in patients with obstructive azoospermia显示文摘This is a retrospective cohort study comparing blastocyst transfer outcomes following intracytoplasmic sperm injection utilizing epididymal versus testicular sperm for men with obstructive azoospermia.All cases at a single center between 2012 and 2016 were included.Operative approach was selected at the surgeon’s discretion and included microepididymal sperm aspiration or testicular sperm extraction.Blastocyst culture was exclusively utilized prior to transfer.The primary outcome was live birth rate.Secondary outcomes included fertilization rate,blastulation rate,euploidy rate,and implantation rate.A mixed effects model was performed.Seventy-six microepididymal sperm aspiration cases and 93 testicular sperm extraction cases were analyzed.The live birth rate was equivalent(48.6%vs 50.5%,P=0.77).However,on mixed effects model,epididymal sperm resulted in a greater likelihood of fertilization(adjusted OR:1.37,95%CI:1.05–1.81,P=0.02)and produced a higher blastulation rate(adjusted OR:1.41,95%CI:1.1–1.85,P=0.01).As a result,the epididymal sperm group had more supernumerary blastocysts available(4.3 vs 3,P<0.05).The euploidy rate was no different.Pregnancy rates were no different through the first transfer cycle.However,intracytoplasmic sperm injection following microepididymal sperm aspiration resulted in a greater number of usable blastocysts per patient.Thus,the true benefit of epididymal sperm may only be demonstrated via a comparison of cumulative pregnancy rates after multiple transfers from one cohort. | Scott J Morin Brent M Hanson Caroline R Juneau Shelby A Neal Jessica N Landis Richard T Scott Jr James M Hotaling | 2020 | Asian Journal of Andrology2020，22，2： | 2 |
| 3 | Clinical genetic testing for male factor infertility: current applications and future directions 显示文摘 | Hotaling J Carrell DT | 2014 | Andrology2014，2，3： | 1 |
| 4 | Deep neck infections显示文摘 | Hotaling A J | 1996 | Am J Otolaryngol1996，17，5： | 1 |
| 5 | The Role of Tonsillectomy in Pediatric IgA Nephropathy 显示文摘 | Mariotti A J Agrawal R Hotaling AJ | 2009 | Arch Otolaryngol Head Neck Surg2009，135，1： | 1 |
| 6 | Comparison of PCR,virus isolation,and indirect fluorescent antibody staining in the detection of naturally occurring feline herpesvirus infections显示文摘 | Burgesser K M Hotaling S Schiebel A | 1999 | J Vet Diagn Invest1999，11，2： | 1 |
| 7 | Effect of collecting duct histol- ogy on renal cell cancer outcbme 显示文摘 | Wright JL Risk MC Hotaling J | 2009 | J Urol2009，182，6： | 1 |
| 8 | Drooling 显示文摘 | Lal D Hotaling A J | 2006 | Curr Opin Otolaryngol Head Neck Surg2006，14，6： | 1 |
| 9 | Long-term NSAID use and incident urothelial cell carcinoma in the VITamins and Lifestyle (VITAL) study显示文摘 | Cheryl Shih James M. Hotaling Jonathan L. Wright Emily White | 2012 | Urologic Oncology: Seminars and Original Investigations2012，，： | 1 |
| 10 | Persistence of urothelial carcinoma of the bladder risk among former smokers: Results from a contemporary, prospective cohort study显示文摘 | Christopher J. Welty Jonathan L. Wright James M. Hotaling Parveen Bhatti Michael P. Porter Emily White | 2012 | Urologic Oncology: Seminars and Original Investigations2012，，： | 1 |
| 11 | Multipath propagation measurements on manufacturing floors at 910 MHz显示文摘 | PAHLAVAN K GANESH R HOTALING T | | 0，，03： | 1 |
| 12 | Male infertility:a risk factor for testicular cancer显示文摘 | Hotaling JM Walsh TJ | | 0，，： | 1 |
| 13 | Risk factors for renal cell carcinoma in the VITAL study显示文摘 | Macleod LC Hotaling JM Wright JL | 2013 | J Urol2013，190，5： | 1 |
| 14 | Risk Factors for Renal Cell Carcinoma in the VITAL Study显示文摘 | Macleod LC Hotaling JM Wright JL | | 0，，05： | 1 |
| 15 | Prostate-speeific antigen (PSA) and PSA velocity for prostate cancer detection in men aged <50 years 显示文摘 | Sun L Moul JW Hotaling ]M | 2007 | BJU Int2007，99，4： | 1 |
| 16 | Predictors of Upper Tract Urothelial Cell Carcinoma After Primary Bladder Cancer: A Population Based Analysis显示文摘 | Jonathan L. Wright James Hotaling Michael P. Porter | 2009 | The Journal of Urology2009，，3： | 1 |
| 17 | An analysis of risk markers in husband to wife violence the current state of knowledge显示文摘 | HOTALING GT SUGARMAN DB | 1986 | Violence Vict1986，1，： | 1 |
| 18 | Predictors of upper tract urothelial cell carcinoma after primary bladder cancer: a population based analysis显示文摘 | Wright JL Hotaling J Porter MP | 2009 | J Urol2009，181，3： | 1 |
| 19 | Age adjusted prostate specific antigen and prostate specific antigen velocity cut points in prostate cancer screening显示文摘 | Moul JW Sun L Hotaling JM | 2007 | J Urol2007，177，2： | 1 |
| 20 | Risk Factors for Renal Cell Carcinoma in the Vitamin and Lifestyle (VITAL) Study显示文摘 | Liam C. Macleod James M. Hotaling Jonathan L. Wright Michael T. Davenport John L. Gore Jonathan Harper Emily White | 2013 | The Journal of Urology2013，，： | 1 |
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