|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Combined detection and subclass characteristics analysis of CTCs and CTECs by SE-iFISH in ovarian cancer显示文摘Objective:Hematogenous metastasis is essential for the progression of ovarian cancer(OC),and circulating tumor cells(CTCs)are part of the metastatic cascade.However,the detection rate of CTC is low due to the use of less sensitive detection methods.Therefore,this study aimed to detect CTCs and circulating tumorigenic endothelial cells(CTECs)in patients with OC using subtraction enrichment and immunostaining and fluorescence in situ hybridization(SE-iFISH).Methods:We enrolled a total of 56 subjects,including 20 OC patients and 36 ovarian benign tumor patients.CTCs and CTECs were captured by subtraction enrichment(SE)and counted and classified according to immunofluorescence staining of tumor markers(TMs)carbohydrate antigen 125(CA125)and human epididymis protein 4(HE4)combined with fluorescence in situ hybridization(iFISH)of chromosome 8(Chr8)aneuploidy.The diagnostic value and subtype characteristics of CTCs and CTECs were investigated.Results:The detection rate of CTCs by SE-iFISH was high.Compared with CA125 and HE4,Chr8 aneuploidy was the major identification feature of CTC.CTC counts in OC were statistically higher than those in benign groups.CTC and CTEC with≥pentaploidy were detected in both groups,illustrating the poor diagnostic value of CTC or CTEC.Distributions of triploid and tetraploid CTC subtypes were significantly different,and combined detection of triploid and tetraploid CTCs showed the best diagnostic value.In contrast,the distribution of CTECs in the OC and benign groups had no statistically significant difference.Small CTCs accounted for over 1/3 of the total CTC count.We also found that small CTCs and CTECs primarily comprised triploid cells,while large CTCs and CTECs mainly comprised pentaploidy and beyond.Conclusions:The application of SE-iFISH offered a more comprehensive understanding of heterogeneous CTCs and CTECs in OC.Analysis of subclass characteristics of the CTCs and CTECs according to Chr8 aneuploidy and cell size may broaden their potential clinical utility and deepen mechanistic studies in OC. | Hongyan Cheng Shang Wang Wenqing Luan Xue Ye Sha Dou Zhijian Tang Honglan Zhu Peter Ping Lin Yi Li Heng Cui Xiaohong Chang | 2021 | Chinese Journal of Cancer Research2021，33，2： | 3 |
| 2 | Anti-synchronization of Two Different Hyperehaotie Systems via Active and Adaptive Control 显示文摘 | ZHANG Xuebing ZHU Honglan | 2008 | International Journal of Nonlinear Science2008，6，3： | 1 |
| 3 | A clinicopathological study of 39 cases of atypical polypoid adenomyoma of the uterus显示文摘Objective:To clarify the clinical and pathological characteristics of atypical polypoid adenomyoma(APA)in order to improve the criteria used to diagnose and treat this disease.Study design:In 39 cases of APA,retrospective analysis was performed of theclinical data,pathological characteristics,and the follow-up findings in patients admitted to the Peking University People's Hospital from 2007 to 2019.Results:The median age was 39.6 years(range 24–60 years).Thirty seven patients were premenopausal(i.e.94.9%)and eighteen patients were nullipara(i.e.46.2%).Abnormal uterine bleeding was the most common presenting symptom.Hysteroscopic transcervical resection(i.e.TCR)identified lesions in 35 cases whereas:(a)hysterectomy identified them in 3 cases;(b)dilatation and curettage(D&C)identified them in 1 case.Immunohistochemical analysis was performed on 24 samples.In the glandular component,hormone receptors were positively expressed.In all cases,Ki67 expression was detectable in approximately 50%of the cases.In those samples,its presence was definitive in eighteen of the nineteen cases(94.7%,18/19),p53 positive expression was present in most cases(68.8%,11/16),and PTEN positive expression was detected in 40%(4/10).Stroma immunophenotype expression was exhibited as follows:(a)CD10-(12/12),Desminþ(6/7);(b)Vimentinþ(4/4);(c)α-SMAþ(3/3)and;d p16 positve staining in of 80%(4/5).The concurrent amount of atypical endometrial hyperplasia with atypical polypoid adenomyoma was 23.1%(9/39),while its concurrent level of endometrial carcinoma with atypical polypoid adenomyoma was 7.7%(3/39).Fertility preserving treatments were performed in 20 patients with strong childbearing desires.Their pregnancy success was 15%(3/20)and the live birth frequency was 10%(2/10).Follow-up findings were available in 36 cases(92.3%)with a medial follow-up of 48.1 months(range 4–122 months).Its pathological recurrence and frequency of progression into endometrial carcinoma were both 5%(1/20).One case died of another type of malignancy,while the other patients were alive.Conclusions:APA occurs mostly during the years of a women's reproductive period.Its diagnosis is based on the analysis of pathological and immunohistochemical findings.Individuals diagnosed with APA are at risk to coexist with endometrial carcinoma and atypical endometrial hyperplasia.For those individuals who desire retaining fertility,the treatment strategy involves performing TCR completely remove the lesions and close follow-up for surveillance of possible progressive APA recurrence.For those individuals who have no fertility desire,hysterectomy may be a preferred option. | Honglan Zhu Taji Bai Zhao Tian Shanshan Lu Dongmei Bao Heng Cui | 2021 | Gynecology and Obstetrics Clinical Medicine2021，1，3： | 1 |
| 4 | Quality evaluation of Po- lygala japonica through simulataneous determination of six bioactive triterpenoid saponins by HPLC -ELSD 显示文摘 | Wang Honglan Gao Jie Zhu Danni | 2007 | J Pharmaceutical and Bi- omedical Analysis2007，43，4： | 1 |
| 5 | Quality evaluation of Polygala japonica through simultaneous determination of six bioactive triterpenoid saponins by HPLC-ELSD显示文摘 | Honglan Wang Jie Gao Danni Zhu Boyang Yu | 2006 | Journal of Pharmaceutical and Biomedical Analysis2006，，4： | 1 |
| 6 | Broad-spectrum and powerful neutralization of bacterial toxins by erythroliposomes with the help of macrophage uptake and degradation显示文摘Anti virulence strategy has been considered as one of the most promising approaches to combat drug-rcesistant bacterial infections.Porc-forming toxins(PFTs)are the largest class of bacterial toxins,inficting their virulence ffect through creating pores on the cell membrane.However,curent solutions for eliminating PFTs are mostly designed based on their molecular structure,requiring customized design for different interactions.In the present study,we employed erythroliposome(denoted as RM-PL),a biomimetic platform constructed by artificial lipid membranes and natural erythrocyle membranes,to necutralize different hemolytic PFTs regardless of their molecular structure.When tested with model PFTs,including a-hemolysin,listeriolysin O,and streptolysin O,RM-PL could completely inhibit toxin-induced hemolysis in a concentration-dependent manner.In vivo studies further confirmed that RM-PL.could eficiently neuralize various toxins and save animals'lives without causing damage to 0rgans or tissues.In addition,we explored the underlying mechanisms of this efficient detoxification ability and found that it was mainly macrophages in the spleen and the liver that took up RM-PL-absorbed toxins through a variety of endocytosis pathways and digested them in lysosomes.In summary,the biomimetic RM-PL presented a promising system for broad-spectrum and powerful toxin neutralization with a mech-anism of lysosome-mediaed loxin degradation. | Chunying Liu Shuangrong Ruan Ying He Xuejing Li Yuefei Zhu Honglan Wang Hanwei Huang Zhiqing Pang | 2022 | Acta Pharmaceutica Sinica B2022，12，11： | 1 |
| 7 | The persistent prevalence and evolution of cross-family recombinant coronavirus GCCDC1 among a bat population:a two-year follow-up显示文摘Bats are connected with the increasing numbers of emerging and re-emerging viruses that may break the species barrier and spread into the human population. Coronaviruses are one of the most common viruses discovered in bats, which were considered as the natural source of recent human-susceptible coronaviruses, i.e. SARS-COV and MERS-CoV. Our previous study reported the discovery of a bat-derived putative cross-family recombinant coronavirus with a reovirus gene p10, named as Ro-BatCoV GCCDC1. In this report, through a two-year follow-up of a special bat population in one specific cave of south China, we illustrate that Ro-BatCoV GCCDC1 persistently circulates among bats. Notably, through the longitudinal observation, we identified the dynamic evolution of Ro-BatCoV GCCDC1 in bats represented by continuously recombination events. Our study provides the first glimpse of the virus evolution in one longitudinally observed bat population cohort and underlines the surveillance and pre-warning of potential interspecies transmittable viruses in bats. | Joseph O.Obameso Hong Li Hao Jia Min Han Shiyan Zhu Canping Huang Yuhui Zhao Min Zhao Yu Bai Fei Yuan Honglan Zhao Xia Peng Wen Xu Wenjie Tan Yingze Zhao Kwok-Yung Yuen William J.Liu Lin Lu George F.Gao | 2017 | Science China(Life Sciences)2017，60，12： | 1 |
| 8 | Spontaneous Vesicle formation in aqueous mixtures of cationic surfactants and partially hydrolyzed polyacrylamide显示文摘 | Huang Jianbin Zhu Ying Zhu Buyao Li Runkai Fu Honglan | 2001 | Journal of Colloid and Interface Science2001，236，： | 1 |
| 9 | Structure and Physicochemical properties in mixed aqueous solution of sodium alkylcarboxylate-alkyltrimethylammonium bromide显示文摘 | Huang Jianbin Zhao Guoxi He Xu Zhu Buyao Fu Honglan | 2000 | Chinese Journal of Chemistry2000，18，3： | 1 |
| 10 | An experimental study on autologous transplantation of fresh ovarian tissue in sheep显示文摘Objective:To investigate current autologous transplantation methods and sites of ovarian tissues in sheep.Methods:Sheep ovaries were resected.Ovarian cortices were sliced and transplanted orthotopically into the ovarian mesangial latum and heterotopically into the greater omentum and under groin skin.The grafts were removed two months after transplantation and examined to evaluate the survival of follicles(hematoxylin-eosin staining)and help determining feasible graft sites and transplantation methods.Results:Graft nodules were found in the transplanted sites.HE staining of the grafts showed that multiple primordial follicles were able to survive in the grafts on both sides of the ovarian mesangial latum,the right side of the greater omentum,and the left inguinal subcutaneous tissue.Secondary or cystic follicles were found in almost all of the grafts.Conclusion:The ovarian mesangial latum,the greater omentum and the inguinal subcutaneous tissue can be used as autologous transplantation sites,where sheep ovarian tissue can survive and the follicles grow and develop in good condition. | Hongyan Cheng Xue Ye Honglan Zhu Yi Li Xudong liang Xiaohong Chang Heng Cui | 2021 | Gynecology and Obstetrics Clinical Medicine2021，1，2： | 0 |
| 11 | Big bright black eyes显示文摘 | Zhu Honglan | 2014 | Chinese Medical Journal2014，，21： | 0 |
| 12 | Nanoplateletsomes for rapid hemostasis performance显示文摘Uncontrollable hemorrhage remains staple trouble in surgical procedures and a leading cause after major trauma.The bleeding issue may trigger various pathologic scenarios that can lead to tissue morbidities and mortalities,and currently available on-site hemostatic agents are confined to a narrow therapeutic index and may carry the risk of immunogenicity.Inspired by the crucial role of platelets in the process of thrombus,a platelet-mimetic plateletsome with wound targeting and blood coagulation properties is developed for hemorrhage control.Plateletsome is formulated by integrating platelet membranes with functionalized synthetic liposomes and exhibits superior wound targeting and effective hemostasis properties.It presents less blood loss and shorter hemostasis time than the platelet membrane vehicles or the conventional liposomes in the mouse tail transection model.The strong homing of the biomimetic plateletsome to the thrombus was also confirmed,demonstrating the potential of this engineered cell membrane vesicle as a biomimetic hemostat for bleeding treatment. | Honglan Wang Yuefei Zhu Longlong Zhang Huiwen Liu Chunying Liu Bo Zhang Yanan Song Yu Hu Zhiqing Pang | 2022 | Chinese Chemical Letters2022，33，6： | 0 |
| 13 | Correspondence Aggressive angiomyxoma of the vagina显示文摘To the editor:Aggressive angiomyxoma (AA) is a very rare soft tissue tumor which affects mainly the soft tissues of the pelvis and perineum in women.As a slow-growing mesenchymal neoplasm,AA tends to infiltrate locally and recur) Here we reported a case of AA of the vagina.The patient was a 36-year-old woman,who presented with a 10 cm×5 cm×5 cm myxoid,well-circumscribed,rubbery mass arising within the vaginal wall and extending to the perineum.Besides a routine clinical work-up,further examinations including ultrasonography and computed tomography (CT) were performed before the operation to evaluate the extent of the disease and reveal the location,relationship and degree of infiltration between tumor and pelvic organs. | Zhu Honglan Wang Shijun | 2014 | Chinese Medical Journal2014，，5： | 0 |
| 14 | Optimal control of a diffusive eco-epidemiological predator-prey model显示文摘In this study,we investigate the optimal control problem for a diffusion eco-epidemiological predator-prey model.We applied two controllers to this model.One is the separation control,which separates the uninfected prey from the infected prey population,and the other is used as a treatment control to decrease the mortality caused by the disease.Then,we propose an optimal problem to minimize the infected prey population at the final time and the cost cause by the controls.To do this,by the operator semigroup theory we prove the existence of the solution to the controlled system.Furthermore,we prove the existence of the optimal controls and obtain the first-order necessary optimality condition for the optimal controls.Finally,some numerical simula-tions are carried out to support the theoretical results. | Xuebing Zhang Guanglan Wang Honglan Zhu | 2020 | International Journal of Biomathematics2020，13，7： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员