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| 1 | Research on Isolation and Clone of Embryonic Stem Cell-Like in Bovine显示文摘Bovine embryonic stem cell would be invaluable for researching the aspect of animal cloning, production transgenic animal and discussion of gene function in vitro. With the object of establishing an effective culture system for isolation and clone of bovine pluripotent stem cell, we cultured bovine embryos and mouse embryos including morula blastula and hatached blastula and obtained animal ICM on Primary marine embryonic fibroblast (Primary murine embryonic fibroblast, PMEF) feeder layer with tissue medium(DMEM supplemented with 15ml/100ml NBS ,0.1μmol/L Na2SeO3, 0. 1mmol/L β-mercaptoethanol, 1 000ng/ml LIF,10 ng/ml IGF, 1mmol/L necessary amino acid and 1mmol/L L-glutamine), then, we obtained mouse ICM and bovine ICM. Moreover, we isolated and cloned the 6 passage bovine ES like cells(12 cell lines) and 9 passage marine ES like cells (52 cell lines) deriving from bovine ICM and murine ICM respectively on the feeder layer of PMEF by disaggregating ICM and ES cell clones of bovine and murine into smaller clumps through digesting with 0. 125g/100ml trypsin and 0.02g/100ml EDTA and scattering with a glass needle. The pluripotency of both murine and bovine ES like cells was identified with morphological character, histochemistry identification, karyotype analysis and differentiation of ES cells in vitro or in vivo. This result showed that bovine embryonic stem cell and murine embryonic stem cell had developmental pluripotency. | AN Li-long, YANG Qi, XIAO Mei, FENG Xiu-Liang, YANG Chun-rong, LEI An-min, GAO Zhi-min, DOU Zhong-ying and QIU Huai( Agriculture College of Zhanjiang Ocean University, Zhanjiang 524008 , P. R . China Key laboratory of AnimalReproductive Endocrinology and Embryo Biotechnology of Agricultural Ministry of China , Northwestern Scienceand Technology University of Agriculture and Forestry, yangling 712100 , P.R. China) | 2002 | Agricultural Sciences in China2002，1，4： | 2 |
| 2 | Porphyry copper-gold mineralization at Yulong, China, pro- moted by decreasing redox potential during magnetite altera- tion显示文摘 | Liang Huaying Sun Weidong Su Wenchao Zartman R E | 2009 | Econ Geol2009，104，4： | 1 |
| 3 | Multiple discrete energy levels and the bistable state of weak anchoring NLC cells显示文摘 | Yang G C Guan R H Huai J X | 2003 | Liquid Crystals2003，30，10： | 1 |
| 4 | Effect of treatment on the tribological behavior of 2D carbon/carbon composites 显示文摘 | Luo R Y Huai X L Qu J W | 2003 | Carbon2003，41，： | 1 |
| 5 | Vasorelaxing and antihy- pertensive effects of 7,8-dihydroxyflavone显示文摘 | HUAI R HAN X WANG B | 2014 | Am J Hyper tens2014，27，5： | 1 |
| 6 | Distinct strategies to make nucleosomal DNA accessible 显示文摘 | Fan Huaying He Xi Kingston R E | 2003 | Molecular Cell2003，11，5： | 1 |
| 7 | Effects of molecular weight, heat- ing rate, synthetic temperature and sintering duration on electro- chemical properties of a LiFePO4/C cathode material pyrolyzed from lithium polyacrylate 显示文摘 | YANG K R HU X B HUAI Y J | 2012 | Journal of Solid State Electroche- mistry2012，，16： | 1 |
| 8 | Adequacy of the in situ observing system in the satellite era of climate SST 显示文摘 | ZHANG HUAI MIN REYNOLDS R W SMITH T M | 2006 | Journal of Atmospheric and Oceanic Technology2006，23，： | 1 |
| 9 | Polymer/colloid dualphase electrolyte membrane for rechargeable lithium batter ies显示文摘 | Huai Y J Deng J M Li R G | 2013 | Journal of Solid State Electrochemistry2013，17，1： | 1 |
| 10 | Correlation of fibrinogen-like protein 2 with disease progression in patients with severe acute pancreatitis显示文摘 | Ye X Huai J Chen R | 2014 | Exp & Therap Med2014，7，1： | 1 |
| 11 | Aryl hydrocarbon receptor nega- tively regulates NLRP3 inflammasome activity by inhibiting NL- RP3 transcription 显示文摘 | Huai W Zhao R Song H | 2014 | Nat Commun2014，205，： | 1 |
| 12 | Probable limited person-to-person transmission of highly pathogenic avian influenza A(H5N1)virus in China显示文摘 | Wang H Feng Z Shu Y Yu H Zhou L Zu R Huai Y Dong J Bao C Wen L Wang H Yang P Zhao W Dong L Zhou M Liao Q Yang H Wang M Lu X Shi Z Wang W Gu L Zhu F Li Q Yin W Yang W Li D Uyeki T M Wang Y | 2008 | Lancet2008，371，9622： | 1 |
| 13 | Numerical Simulation on Enhancement of Natural Convection Heat Transfer by Acoustic Cavitation in a Square Enclosure显示文摘 | CAI J HUAI X L YAN R S | 2009 | Applied Thermal Engineering2009，29，10： | 1 |
| 14 | Risk factors for human illness with avian influenza A(H5N1)virus infection in China显示文摘 | Zhou L Liao Q Dong L Huai Y Bai T Xiang N Shu Y Liu W Wang S Qin P Wang M Xing X Lv J Chen R Y Feng Z Yang W Uyeki T M Yu H | 2009 | J Infect Dis2009，199，12： | 1 |
| 15 | Resource management in the next generation DS-CDMA cellular networks显示文摘 | ZHANG J H HUAI J P XIAO R Y LI Bo | 2004 | IEEE Communications Magazine2004，，： | 1 |
| 16 | Aryl hydrocarbon receptor negatively regulates NLRP3 inflammasome activity by inhibiting NLRP3 transcription显示文摘 | Huai W Zhao R Song H | 2014 | Nat Commun2014，5，： | 1 |
| 17 | 7,8-二羟基黄酮的舒血管和降压作用显示文摘虽已有研究证明7,8-二羟基黄酮[7,8-dihydroxyflavone,(7,8-DHF)]具有有效的神经保护作用,但其对血管功能的作用还不是很明确。方法:通过从正常大鼠分离的主动脉环,检测7,8-DHF对由肾上腺素诱导的动脉收缩的影响。应用阻断剂、Western blot和原代培养血管平滑肌细胞研究其影响机制。用尾套法测量大鼠的血压。结果:7,8-DHF剂量依赖性扩张肾上腺素预收缩的内皮细胞完整的主动脉环,50%最大效应(EC50)浓度约24μmol/L。左旋硝基精氨酸甲酯盐酸盐(一氧化氮合酶抑制剂)和ODQ(1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one,可溶性鸟苷酸环化酶受体阻滞剂)均显著降低7, | Huai R Han X Wang B Li C Niu Y Li R Qu Z | 2014 | 中华高血压杂志2014，22，8： | 0 |
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