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| 1 | DAPT Enhances the Apoptosis of Human Tongue Carcinoma Cells显示文摘Aim To investigate the effect of DAPT(γ-secretase inhibitor) on the growth of human tongue carcinoma cells and to determine the molecular mechanism to enable the potential application of DAPT to the treatment of tongue carcinoma. Methodology Human tongue carcinoma Tca8113 cells were cultured with DAPT.Cell growth was determined using Indigotic Reduction method.The cell cycle and apoptosis were analyzed by flow cytometry.Real-time PCR and Immuno-Fluorescence(IF) were employed to determine the intracellular expression levels. Results DAPT inhibited the growth of human tongue carcinoma Tca8113 cells by inducing G_0-G_1 cell cycle arrest and apoptosis.The mRNA levels of Hairy/Enhancer of Split-1(Hes-1),a target of Notch activation,were reduced by DAPT in a dose-dependent manner.Coincident with this observation,DAPT induced a dose-dependent promotion of constitutive Caspase-3 in Tca8113 cells. Conclusion DAPT may have a therapeutic value for human tongue carcinoma.Moreover,the effects of DAPT in tumor inhibition may arise partly via the modulation of Notch-1 and Caspase-3. | Brian E. Grottkau Xi-rui Chen Claudia C. Friedrich Xing-mei Yang Wei Jing Yao Wu Xiao-xiao Cai Yu-rong Liu Yuan-ding Huang Yun-feng Lin | 2009 | International Journal of Oral Science2009，1，2： | 7 |
| 2 | Osteogenesis of Adipose-Derived Stem Cells显示文摘Current treatment options for skeletal repair,including immobilization,rigid fixation,alloplastic materials and bone grafts,have significant limitations.Bone tissue engineering offers a promising method for the repair of bone deficieny caused by fractures,bone loss and tumors.The use of adipose derived stem cells(ASCs) has received attention because of the self-renewal ability,high proliferative capacity and potential of osteogenic differentiation in vitro and in vivo studies of bone regeneration.Although cell therapies using ASCs are widely promising in various clinical fields,no large human clinical trials exist for bone tissue engineering.The aim of this review is to introduce how they are harvested,examine the characterization of ASCs,to review the mechanisms of osteogenic differentiation,to analyze the effect of mechanical and chemical stimuli on ASC osteodifferentiation,to summarize the current knowledge about usage of ASC in vivo studies and clinical trials,and finally to conclude with a general summary of the field and comments on its future direction. | Brian E. Grottkau Yunfeng Lin | 2013 | Bone Research2013，1，2： | 6 |
| 3 | Comparison of Effects of Mechanical Stretching on Osteogenic Potential of ASCs and BMSCs显示文摘Mechanical forces play critical roles in the development and remodeling processes of bone.As an alternative cell source for bone engineering,adipose-derived stem cells(ASCs)should be fully investigated for their responses to mechanical stress.Similarly,the osteogenic potential,stimulated by mechanical stress,should be compared with bone marrow stromal cells(BMSCs),which have been clinically used for bone tissue engineering.In this study,ASCs and BMSCs were osteogenic-induced for 48 hours,and then subjected to uniaxial mechanical stretching for 2 or 6 hours.Cell orientation,osteogenic regulatory genes,osteogenic genes and ALP activities were measured and compared between ASCs and BMSCs.ASCs could align in a perpendicular way to the direction of stretching stress,while BMSCs did not present a specific alignment.Both 2 and 6 hours mechanical stretching could enhance the mRNA expression of Osx and Runx2 in BMSCs and ASCs,while OCN mRNA only increased in ASCs after 6 hours mechanical loading.Mechanical stretching enhanced the BMP-2 mRNA expression in ASCs,while only after 6 hours of mechanical loading significantly increased the BMP-2 gene expression in BMSCs.Significant differences only exist between ASCs and BMSCs loaded at 2 hours of mechanical stretching.It is concluded that ASCs are more rapid responders to mechanical stress,and have greater potential than BMSCs in osteogenesis when stimulated by mechanical stretching,indicating their usefulness for bone study in a rat model. | Brian E. Grottkau Xingmei Yang Liang Zhang Ling Ye Yunfeng Lin | 2013 | Bone Research2013，1，3： | 4 |
| 4 | Compartment syndrome in children and adolescents显示文摘 | Grottkau BE | 2005 | J Pediatr Surg2005，40，： | 1 |
| 5 | MDGT of the elbow in pediatric patients with posttraumatic elbow effusions 显示文摘 | Chapman V Grottkau B Albright M | 2006 | AJR2006，187，3： | 1 |
| 6 | Discoid lateral meniscus in children 显示文摘 | Hart ES Kalra KP Grottkau BE | 2008 | Orthop Nurs2008，3，： | 1 |
| 7 | MDCT of the elbow in pediatric patients with posttraumatic elbow effusions显示文摘 | Chapman V Grottkau B Albright M | 2006 | AJR Am J Roentgenol2006，187，3： | 1 |
| 8 | MDCT of the elbow in pediatric patients with posttraumatic elbow effusions显示文摘 | Chapman V Grottkau B Albright M | 2006 | Am J Roentgenol2006，187，3： | 1 |
| 9 | Coaptive Film Versus Subcuticular Suture: Comparing Skin Closure Time Following Identical, Single-session, Bilateral Limb Surgery in Children显示文摘 | Gleeson Rebello Ravi Parikh Brian Grottkau | 2009 | Journal of Pediatric Orthopaedics2009，，6： | 1 |
| 10 | Current concepts in the treatment of hallux valgus显示文摘 | Hart ES deAsla RJ Grottkau BE | 2008 | Orthop Nurs2008，27，5： | 1 |
| 11 | DAPT enhances th apoptosis of human tongue carcinoma ceils 显示文摘 | Grottkau BE Chen XR Friedrich CC | 2009 | Int J Oral Sci2009，1，2： | 1 |
| 12 | The newborn foot: diagnosis and management of common conditions显示文摘 | Hart ES Grottkau BE Rebello GN | 2005 | Orthop Nurs2005，24，5： | 1 |
| 13 | Muhide- tector computed tomography of pediatric lateral condylar fractures 显示文摘 | Chapman VM Grottkau BE Albright M | 2005 | J Comput Assist Tomogr2005，29，6： | 1 |
| 14 | Muitidetector computed tomography of fracture - separation of the distai humeraI epiphysis 显示文摘 | Chapman VM AIbright M Grottkau BE | 2005 | J Comput Assist Tomogr2005，29，： | 1 |
| 15 | MDCT of the elbow in pediatric patients with posttraumatic elbow effusions显示文摘 | Chapman V Grottkau B Albright M et aI | 2006 | Am J Roentgenol2006，187，3： | 1 |
| 16 | Broken bones: common pediatric lower extremity fractures-Part Ⅲ 显示文摘 | Hart ES Luther B Grottkau BE | 2006 | Orthop Nurs2006，25，6： | 1 |
| 17 | DAPT enhances the apoptosis of human tongue carcinoma cells显示文摘 | Grottkau BE Chen XR Friedrich CC Yang XM Jing W Wu Y Cai XX Liu YR Huang YD Lin YF | | 0，，2： | 1 |
| 18 | MDCT of the elbow in pediatric patients with posttraumatic elbow effusions 显示文摘 | Chapman V Grottkau B Albright M | 2006 | Am J Roentgenol2006，187，3： | 1 |
| 19 | Bone marrow derived pluripotent cells are pericytes which contribute to vascularization显示文摘 | Cai X Lin Y Friedrich CC Neville C Pomerantseva I Sundback CA Zhang Z Vacanti JP Hauschka PV Grottkau BE | | 0，，： | 1 |
| 20 | Adipose stem cells originate from perivascular cells显示文摘 | Cai X Lin Y Hauschka PV Grottkau BE | | 0，，： | 1 |
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