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| 1 | Multipotent stromal cells stimulate liver regeneration by influencing the macrophage polarization in rat显示文摘AIM To investigate the influence of the umbilical cordderived multipotent stromal cells(MSCs) on recovery of the liver after the subtotal resection, that is, removal of 80% of the organ mass, a renowned model of the small-for-size liver remnant syndrome. METHODS The MSCs were obtained from the intervascular tissue of umbilical cords, dissected from rat fetuses, by the explant culture technique. The vital labeling of MSCs with РКН26 was carried out on the 3 rd passage. The subtotal resection was performed on male Sprague-Dawley rats. The experimental group animals received a transplant 106 MSCs infused into the spleen. Hepatocyte proliferation was assessed by counting of either mitotic figures or Ki67-positive cells in microscopic images. MSC differentiation was assessed with antibodies to hepatocyte-specific marker cytokeratin 18(CK18), cholangiocyte-specific protein CK19, smooth muscle cell-specific protein α-SMA, the endothelial cell marker CD31, or the active fibroblast marker FAPα. Total macrophages of the liver were selectively stained in cryosections incubated with antiCD68 antibodies(1:100, Abcam), while the M2 a and M2 c macrophage populations were selectively stained with anti-CD206 antibodies. Expression of interleukin and growth factor genes was evaluated with PCR-RT.RESULTS Intrasplenic allogeneic transplantation of the umbilical cord-derived multipotent stromal cells stimulates reparative processes within the residual liver tissue after subtotal resection(removal of 80% of the organ mass), as indicated by increased rates of hepatocyte proliferation and accelerated organ mass recovery. These effects may result from paracrine influence of the transplanted cells on the resident macrophage population of the liver. The transplantation favors polarization of macrophages to M2 phenotype(the M2-polarized macrophages specifically express CD206; they are known to suppress inflammation and support tissue repair). No differentiation of the transplanted cells into any of the liver cell types have been observed in the study.CONCLUSION We found no direct evidence for the paracrine effect of MSCs on liver regeneration after the subtotal liver resection in rats. However, the paracrine mechanism of the therapeutic activity of transplanted MSC is indirectly indicated by a decrease in the total number of CD68 + macrophages and an increase in the proportion of M2 pro-repair macrophages in the regenerating liver as compared to animals in which the transplantation was only mimicked. | Andrey Elchaninov Timur Fatkhudinov Natalia Usman Irina Arutyunyan Andrey Makarov Anastasia Lokhonina Irina Eremina Viktor Surovtsev Dmitry Goldshtein Galina Bolshakova Valeria Glinkina Gennady Sukhikh | 2018 | World Journal of Hepatology2018，10，2： | 2 |
| 2 | Renal impairment among post- menopausal women with osteoporosis from a large health plan in Israel显示文摘 | Yu'J Goldshtein I Shalev V | 2015 | Arch Osteoporos2015，10，1： | 1 |
| 3 | Agglomeration of submicrometer particles in weak periodic shock waves显示文摘 | Shuster K Fichman M Goldshtein A | 2002 | Physics of Fluids2002，14，5： | 1 |
| 4 | Isolation and phenotypical characterization fo mesenchymal stem cells from human fetal thymus显示文摘 | Rzhaninova AA Gornostaeva SN Goldshtein DV | 2005 | Bull Exp Biol Med2005，139，1： | 1 |
| 5 | Renal impairment among postmenopausal women with osteoporosis from a large health plan in Israel显示文摘 | Yu J Goldshtein I Shalev V | 2015 | Archives of Osteoporosis2015，10，1： | 1 |
| 6 | Europ Polym J 显示文摘 | Jenny Goldshtein Shlomo Margel | 2009 | 45(11):2987-29952009，45，11： | 1 |
| 7 | Some peculiarities of the structure of high boron steels显示文摘 | Goldshtein Ya E and Mizin V G | 1989 | Metal Science and Heat Treatment1989，30，7： | 1 |
| 8 | Expression ofNMDA receptors in multipotent stromal cells of human adipose tissueunder conditions of retinoic acid-induced differentiation 显示文摘 | Kulikov AV Rzhaninova AA Goldshtein DV | 2007 | BullEjcpBiol Med2007，144，4： | 1 |
| 9 | Advanced multiphase silicon-based anodes for high-energy-density Li-ion batteries显示文摘 | Goldshtein K Freedman K Schneier D | 2015 | Journal of the Electrochemical Society2015，162，6： | 1 |
| 10 | Pathways of Bone Marrow Mononuclear Cell Differentiation after Transplantation into Postinfarction Heart显示文摘 | J. P. Baikova T. Kh. Fatkhudinov G. B. Bolshakova T. B. Buharova T. K. Dubovaya L. V. Kaktursky D. V. Goldshtein | 2011 | Bulletin of Experimental Biology and Medicine2011，，1： | 1 |
| 11 | Synthesis and characterization of new flame-retardant microspheres by dispersion polymerization of pentabromobenzyl acrylate 显示文摘 | Jenny Goldshtein Shlomo Margel | 2009 | European Polymer Journal2009，45，11： | 1 |
| 12 | Clinical study of the efficiency of combined cell transplant on the basis of multipotent mesenchymal stromal adipose tissue cells in patients with pronounced deficit of the maxillary and mandibulary bone tissue 显示文摘 | KULAKOV AA GOLDSHTEIN DV GRIGORYAN AS et al | 2008 | Bull Exp Biol Med2008，146，4： | 1 |
| 13 | Isolation and phe- notypical characterization of mesenchymal stem cells from human fe- tal thymus 显示文摘 | Rzhaninova AA Gomostaeva SN Goldshtein DV | 2005 | Bull ExpBiol Med2005，139，11： | 1 |
| 14 | System decomposition technique for spray modeling in CFD codes 显示文摘 | Bykov V Goldfarb I Goldshtein V | 2007 | Computers Fluids2007，36，： | 1 |
| 15 | Some peculiarities of the stucture high boron steels显示文摘 | Goldshtein Ya E Mizln V G | 1989 | Metal Science and Heat Treatment1989，30，7： | 1 |
| 16 | Some peculiarities of the structure of high boron steels 显示文摘 | Goldshtein Ya E Mizin V G | 1989 | Metal Science and Heat Treatment1989，30，7： | 1 |
| 17 | Fractal cracks 显示文摘 | Goldshtein R V Mosolov A B | 1992 | Journal Applied Mathematics and Mechanics1992，56，4： | 1 |
| 18 | Clinical study of the efficiency of combined cell transplant on the basis of multipotent mesenchymM stromal adipose tissue cells in patients with pronounced deficit of the maxillary and mandibulary bone tissue显示文摘 | Kulakov AA Goldshtein DV Grigoryan AS | 2008 | Bull Exp Biol Med2008，146，4： | 1 |
| 19 | Some peculiarities of the structure of high boron steels显示文摘 | GOLDSHTEIN Y E MIZIN V G | 1989 | Metal Science and Heat Treatment1989，30，78： | 1 |
| 20 | Clinical Study of the Efficiency of Combined Cell Transplant on the Basis of Multipotent Mesenchymal Stromal Adipose Tissue Cells in Patients with Pronounced Deficit of the Maxillary and Mandibulary Bone Tissue显示文摘 | A. A. Kulakov D. V. Goldshtein A. S. Grigoryan A. A. Rzhaninova I. S. Alekseeva I. V. Arutyunyan A. V. Volkov | 2008 | Bulletin of Experimental Biology and Medicine2008，，4： | 1 |
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