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| 1 | Proteomic response analysis of endothelial cells of human coronary artery to stimulation with carbachol显示文摘AIM: To identify the molecular basis of the endothelial target for acetylcholine (ETA). METHODS: Proteomic methods were used to monitor changes in protein expression in the first 10h following the stimulation of human coronary endothelial cells with carbachol 100μmol/L. Thirty proteins showing the largest changes were identified by mass spectrometry. RESULTS: Based on analysis with Image Master 2-D Elite software, about 623 protein spots were detected in control cells and 825 protein spots in carbachol-treated cells, the matching rate was 68.1%. Among all the detected spots, 39 were up-regulated and 29 were down-regulated, showing detectable changes varied from 1.7-3.8 folds. Forty one spots in the peptide mass fingerprints were successfully obtained. The most interesting feature was that all the four newly synthesized proteins belonged to the heat shock protein family.CONCLUSION: These identified proteins played key roles in the molecular mechanism of ETA. | MinYU Dong-meiCHEN GangHU HaiWANG | 2004 | Acta Pharmacologica Sinica2004，25，9： | 3 |
| 2 | 6-Hydroxydopamine-induced glutathione alteration occurs via glutathione enzyme system in primary cultured astrocytes显示文摘Aim: To define the role of enzymes involved in glutathione metabolism in 6-hydroxydopamine (6-OHDA)-induced glutathione alteration in primary cultured astrocytes. Methods: Total glutathione (GSx) levels were determined using the modified enzymatic microtiter plate assay. The mRNA levels of T-glutamylcyste inesynthetase (γGCS), T-glutamyltransferase (γGT), glutathione peroxidase (GPx), GR (glutathione reductase), and glutathione transferases (GST) were determined using RT-PCR. γGT activity was determined using γGT assay kits. Results: In primary cultured astrocytes, 6-OHDA induced a significant elevation of cellular GSx levels after treatment for 24 h. However, the GSx levels decreased after 24 h and the values were even lower than the value in the control group without 6-OHDA at 48 h. RT-PCR data showed that the mRNA levels of γGCS, the ratelimiting enzyme of γ-L-glutamyl-L-cysteinylglycine (GSH) synthesis, were increased by 6-OHDA after treatment for 24 h and 48 h; the mRNA levels of GPx, GR, and GST did not alter in 6-OHDA-treated astrocytes after treatment for 24 h and 48 h; and 6-OHDA increased the mRNA levels and the activity of γGT after treatment for 48 h, which induced a decrease in GSx levels, despite the up-regulation of γGCS aftere xposure to 6-OHDA for 48 h. Conclusion: The change in γGCS correlated with the increase in GSH levels induced by 6-OHDA after treatment for 24 h. GSx levels decreased because of increased γGT mRNA levels and γGT activity induced by 6-OHDA after treatment for 48 h. | JiZHANG JunHU Jian-huaDING Hong-hongYAO GangHU | 2005 | Acta Pharmacologica Sinica2005，26，7： | 2 |
| 3 | ATP‐sensitive potassium channels: A promising target for protecting neurovascular unit function in stroke显示文摘 | Xiu‐LanSun GangHu | 2010 | Clinical and Experimental Pharmacology and Physiology2010，，： | 1 |
| 4 | Hepatecyte regeneration after partial liver irradiation in rots显示文摘 | Zhao Jiandong Guo Liangjiang Wei Ganghu | 2009 | Experimental and Toxicologic Pathology2009，61，： | 1 |
| 5 | Inhibitory effects of 1-methyl-4-phenylpyridinium on glutamate uptake into cultured C6 glioma cells显示文摘AIM: To investigate the effect of 1-methyl-4-phenylpyridinium (MPP+) on the glutamate uptake into cultured C6 glioma cells. METHODS: The glutamate uptake into C6 glioma cells was measured by radio-ligand binding assay method. The effect of MPP+ on the morphology of C6 glioma cells was observed under phase contrast microscopy; apoptosis of C6 glioma cells were measured by FITC-labeled Annexin V staining and flow cytometry. Cell viability was measured by MTT method. RESULTS: MPP+inhibited glutamate uptake into C6 glioma cells. However, MPP+ failed to induce any morphological changes of C6 glioma cells, and exposure to MPP+ had no effect on the viability and the apoptotic percentage of C6 glioma cells. Incubation with 12-O-tetradecanoylphorbol -13-acetate (TPA), a protein kinase C activator, caused a significant increase in glutamate uptake and completely reversed MPP+-induced inhibitory effect on glutamate uptake. CONCLUSION: The present results indicate that glutamate transporters mayhave important pathogeneticimplications in Parkinson disease. MPP+-inducedinhibition ofglutamate uptake was due to the dysfunction of glutamate transporters; TPA enhanced glutamate uptake and completely reversed the inhibitory effect of MPP+. | Hong-hongYAO Jian-huaDING Hai-rongHE GangHU | 2004 | Acta Pharmacologica Sinica2004，25，7： | 1 |
| 6 | Analysis of direct action fuzzy PID controller structures显示文摘 | MANN G K I BAO Ganghu GOSINE R G | 1999 | Sys- tems Man and Cybernetics Part B:Cybemeties IEEE Transactions on1999，29，3： | 1 |
| 7 | Activation of astroglial group Ⅱ and Ⅲ metabotropic glutamate receptors protects midbrain neurons against LPS or MPP^+ -induced neurotoxicity显示文摘AIM: Activation of glial metabotropic glutamate receptors (mGluRs) may be proved to play a critical role for neuroprotection in neurodegenerative diseases. Excess glutamate induced-excitoxicity is implicated in the initiation or progression of the neurodegenerative process. Glutamate accumulation in the central nervous system mediated by inhibiting | Hong-HongYao FangWang FangZhou Li-FangHu TaoSun Jian-HuaDing GangHu | 2004 | 中国药理通讯2004，21，2： | 0 |
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