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| 1 | Mechanical properties of hepatocellular carcinoma cells显示文摘AIM: To study the viscoelastic properties of humanhepatocytss and helatocellulsr carcinoma (HCC) cellsunder cytoslelstal perturbation, and to further to study theviscoelastic properties and the adhesive properties of mousehepatorna cells (HTC) in different cell cycls.METHODS: Micropipette aspiration technique was adopted tomeasure viscoelastic coefficients and adhesion force tocollagen coated surface ofthe cells. Three kinds ofcytoskeleton perturbing agents, colchiclnes (Col),cytochalssin D (CD) and vinblastine (VBL), were used totreat HCC cells and hepatocytes and the effects of thesetreatent on cell viscoelastic coefficients were investigated.The experimental results were analyzed with a thres-elsmentstandard linear solid. Further, the viscoelastic properties ofHTC cells and the adhesion force of different cycle HTC cellswere also investigated. The synchronous G1 and S phasecells were achieved through thymine-2-desoryriboside andcolchicines sequential blockage method and thymine-2-desoryriboside blockage method respectively.RESULTS: The elastic coefficients, but not viscouscoefficient of HCC cells (k1 = 103.6± 12.6N.m-2, k2 =42.5±10.4N. m-2, μ = 4.5 ± 1.9Pa. s), were significantly higherthan the corresponding value for hepatocytes (K1 = 87.5 ±12.1N.m-2, k2 =33.3± 10.3N.m-2, μ=5.9±3.0Pa. s, P<0.01). Upon treatment with CD, the viscoelastic coefficients ofboth hepotocytes and HCC cells decreased consistently,with magnitudes for the decrease in elastic coefficients ofHCC cells (k1: 68.7 N.m-2 to 81.7N.m-2, 66.3 % to 78.9 %;k2: 34.5 N.m-2 to37.1N.m-2, 81.2% to 87.3 %, P<0.001)larger than those for normal hepatocytes (k1: 42.6N. m-2 to49.8N.nt-2, 48.7% to56.9 %; k2: 17.2N.m-2 to 20.4N.m-2,51.7 % to 61.3 %, P< 0.001). There was a little decrease inthe vlscous coefficient of HCC cells (2.0 to 3.4Pa. s, 44.4 to75.6 %, P<0.001) than that for hepatocytes (3.0 to 3.gPa.s, 50.8to 66.1% P<0.001). Upon trastment with Col andVBL, the elastic coefficients of hepatocytes generallyincreased or tended to increase while those of HCC cellsdecreased. HTC cells with 72.1% ofG1 phase and 98. 9 % ofS phase were achieved and high K1, k2 value and low μvalue were the general characlteristics of HTC cells. G1phase cells had higher K1 value and lower tμ value than Sphase cells had, and G1 phase HTC cells had strongeradhesive forces [(275.9±232.8) x 10-10N] than S phase cells[(161.2± 120.4) x l0-10N, P<0.001).CONCLUSION: The difference in both the pattern and themagnitude of the effect of cytoskeletal perturbing agent onthe viscoelastic properties between HCC cells andhepatocytes may reflect differencss in the state of thecytusieleton structure and function and in the sensitivity toperturbing agent treatment between trinse two types of cells.Change in the viscoelastic properties of cancer cells mayaffect significantly tumor cell invasion and metastasis as wellas interactions between tumor cells and their micro-mechanical environments. | Gang Zhang,Department of Pathophysiology,The Third Military Medical University,Chongqing 400038,China Mian Long Zhe-Zhi Wu Wei-Qun Yu,College of Bioengineer,Chongqing university,Chongqing 400044,China | 2002 | World Journal of Gastroenterology2002，8，2： | 19 |
| 2 | Effectiveness of Different Waist Circumference Cut-off Values in Predicting Metabolic Syndrome Prevalence and Risk Factors in Adults in China显示文摘Objective To study the effectiveness of waist circumference cut-off values in predicting the prevalence of metabolic syndrome(MetS) and risk factors in adults in China. Methods A cross-sectional survey was condcuted in 14 provinces(autonomous region, municipality) in China. A total of 47 325 adults aged ≥20 years were selected by multistage stratified sampling, and questionnaire survey and physical and clinical examination were conducted among them. MetS was defined according to the International Diabetes Federation(IDF) criteria and modified IDF criteria.Results The age-standardized prevalence of MetS was 24.2%(22.1% in men and 25.8% in women) and 19.5%(22.1% in men and 18.0% in women) according to the IDF criteria and modified IDF criteria respectively. The age-standardized prevalence of pre-MetS was 8.1%(8.6% in men and 7.8% in women) according to the modified IDF criteria. The prevalence of MetS was higher in urban residents than rural residents and in northern China residents than in southern China residents. The prevalence of central obesity was about 30% in both men and women according to the ethnicity-specific cut-off values of waist circumference for central obesity(90 cm for men and 85 cm for women). Multivariate regression analysis revealed no significant difference in risk factors between the two MetS definitions. Conclusion Using both the modified IDF criteria and ethnicity-specific cut-off values of waist circumference can provide more useful information about the prevalence of MetS in China. | ZHOU Hai Cheng LAI Ya Xin SHAN Zhong Yan JIA Wei Ping YANG Wen Ying LU Ju Ming WENG Jian Ping JI Li Nong LIU Jie TIAN Hao Ming JI Qiu He ZHU Da Long CHEN Li GUO Xiao Hui ZHAO Zhi Gang Li Qiang ZHOU Zhi Guang GE Jia Pu SHAN Guang Liang | 2014 | Biomedical and Environmental Sciences2014，27，5： | 17 |
| 3 | Recent advances in gel polymer electrolyte for high-performance lithium batteries显示文摘Lithium batteries (LBs) have become increasingly important energy storage systems in our daily life. However, their practical applications are still severely plagued by the safety issues from liquid electrolyte, especially when the batteries are exposed to mechanical, thermal, or electrical abuse conditions. Gel polymer electrolytes (GPEs) are being considered as an effective solution to replace currently available organic liquid electrolyte for building safer LBs. This review provides recent advancements in GPEs applied for high-performance LBs. On the one hand, from the environmental and economic point of view, the skeletons of GPEs changed from traditional polymer to renewable and degradable polymer. On the other hand, in addition to being as a component with good electrochemical and physical characterizations, the GPEs also need to provide some functions for addressing the concerns of lithium (Li) dendrites, unstable cathode electrolyte interface, dissolution and migration of transition metal ions,'shuttle effect' of polysulfides, and so on. Finally, to synchronously meet the challenges from the advanced cathode and Li metal anode, the bio-based GPEs with multi-functionality are proposed to develop high-energy/powerdensity batteries in the future. | Ming Zhu Jiaxin Wu Yue Wang Mingming Song Lei Long Sajid Hussain Siyal Xiaoping Yang Gang Sui | 2019 | Journal of Energy Chemistry2019，28，10： | 17 |
| 4 | Expression and localization of CKLFSF2 in human spermatogenesis显示文摘瞄准:调查表示并且代替细胞的本地化象 ofchemokine 一样因素总科 2 (CKLFSF2 ) 在在精子发生的人的睾丸和它的潜在的角色。方法:对 CKLFSF2 的特定的 polyclonal 抗体被提起。表示和在生精的小管的 CKLFSF2 的细胞的本地化是检查 byimmu-nohistochemistry 方法。另外,在 situ,杂交被使用本地化 mRNAdistribution。EGFP-CKLFSF2 熔化蛋白质在 COS-7 房间被表示在 vitro 本地化 itssubcellular 地点。另外,在有男不孕的病人的睾丸的 CKLFSF2 的反常表示是由反向的抄写聚合酶链反应(RT-PCR ) 的 assayed andimmunohistochemistry 方法。结果:与精子发生有靠近的关联背叛, CKLFSF2 明确地在 meiotic 和 post-meiotic 细菌房间被表示,它对 endoplasmic 蜂窝胃局部性(嗯) 在 Golgi 仪器附近。结论:CKLFSF2 能在成熟分裂和精子形式的过程起重要作用,并且可能在 endoplasmic 蜂窝胃涉及小囊的运输或膜并置事件。 | Gang Liu Zhong-Cheng Xin Liang Chen Long Tian Yi-Ming Yuan Wei-Dong Song Xue-Jun Jiang Ying-Lu Guo | 2007 | Asian Journal of Andrology2007，9，2： | 16 |
| 5 | NVP-BEZ235, a novel dual PI3K/mTOR inhibitor, enhances the radiosensitivity of human glioma stem cells in vitro显示文摘 | Wen-juan WANG Lin-mei LONG Neng YANG Qing-qing ZHANG Wen-jun JI Jiang-hu ZHAO Zheng-hong QIN ZhongWANG Gang CHEN Zhong-qin LIANG | 2013 | Acta Pharmacologica Sinica2013，34，5： | 14 |
| 6 | Cox7a2 mediates steroidogenesis in TM3 mouse Leydig cells显示文摘瞄准:为了在 steroidogenesis 和机制上调查 Cox7a2 的规章的功能,在 TM3 老鼠 Leydig 房间包含了。方法:Cox7a2 的 cDNA 从 TM3 老鼠 Leydig 房间被克隆。它是短暂基因 transfection 在表达式上为 Cox7a2 克隆到回来的 pDsRed-Express-N1 和 transfected 进 TM3 鼠标 Leydig 房间的潜水艇。Steroidogenesis 影响了由在表示 Cox7a2 上被 ELISA 学习。得到这效果的机制, steroidogenic 的表示尖锐规章(星) 蛋白质和反应的氧种类(ROS ) 被西方的污点和氟量计分别地检验。结果:Cox7a2 (249 bp ) 的 cDNA 从 Leydig 房间被克隆并且由定序的 DNA 证实了。在构造 pDsRed-Express-N1-Cox7a2 是回来的 transfected 进 TM3 老鼠 Leydig 房间以后, Cox7a2 禁止了导致的 luteinizing (LH ) 不仅睾丸激素的分泌物而且星蛋白质的表示。同时, Cox7a2 在 TM3 老鼠 Leydig 房间增加了 ROS 的活动。结论:Cox7a2 禁止了导致 LH 的星蛋白质表示,和作为结果的睾丸激素生产,至少部分地,由在 TM3 鼠标 Leydig 房间增加 ROS 活动。 | Liang Chen Zhong-Cheng Xin Xin Li Long Tian Yi-Ming Yuan Gang Liu Xue-Jun Jiang Ying-Lu Guo | 2006 | Asian Journal of Andrology2006，8，5： | 13 |
| 7 | Laparoscopic hepaticoplasty using gallbladder as a subcutaneous tunnel for hepatolithiasis显示文摘AIM:To investigate the feasibility,efficacy and safety of laparoscopic hepaticoplasty using gallbladder as subcutaneous tunnel and sphincter-of-Oddi preservation for hepatolithiasis.METHODS:From January 2010 to July 2013,six patients with hepatolithiasis were treated at our institution.All the patients underwent laparoscopic surgery.The procedures included common hepatic duct exploration,stone clearance by fiberoptic choledochoscopy,hilar bile duct hepaticoplasty with preservation of the sphincter of Oddi,anastomosis between the hilar bile duct and neck of the gallbladder,and establishment of a subcutaneous tunnel with the gallbladder.Two patients underwent left lateral hepatectomy simultaneously.Clinical data including operation time,intraoperative blood loss,operative morbidity,hospital mortality,stone clearance,and recurrence rate were analyzed.RESULTS:All patients successfully completed laparoscopic surgery.The mean length of hospital stay was4.5±0.9 d(range:3-6 d).The mean blood loss of the hepatectomy was 450 mL(range:200-700 mL),and the blood loss of the other four was 137±151 mL (range:50-400 mL).The mean operative time was 318±68 min(range:236-450 min).The operative morbidity and hospital mortality were zero.The immediate stone clearance rate was 100%.All patients were followed up for an average of 17 mo(range:7-36 mo).One of the six patients had abdominal mass with pain,and subcutaneous tunnel cholangiography showed severe gallbladder-biliary anastomotic stricture at 4 mo postoperatively.There was no stone recurrence and no cholangitis during follow-up.CONCLUSION:Laparoscopic hepaticoplasty using gallbladder with a subcutaneous tunnel and preserving the sphincter of Oddi is feasible,safe and effective for hepatholithiasis. | Long Cui Zhi Xu Xiao-Feng Ling Li-Xin Wang Chun-Sheng Hou Gang Wang Xiao-Si Zhou | 2014 | World Journal of Gastroenterology2014，20，12： | 13 |
| 8 | A study on arsenic trioxide inducing in vitro apoptosis of gastric cancer cell lines显示文摘INTRODUCTION Cell apoptosis,which involves the biologic regulation of the numbers and vital activity of cells,is an important metaboloc process in both normal cells and tumor cells. | Qin Long Gu Ning Li Li Zheng Gang Zhu Hao Ran Yin Yan Zhen Lin | 2000 | World Journal of Gastroenterology2000，6，3： | 12 |
| 9 | Recent Progress in Dual-Polarization Radar Research and Applications in China显示文摘Dual-polarization(dual-pol)radar can measure additional parameters that provide more microphysical information of precipitation systems than those provided by conventional Doppler radar.The dual-pol parameters have been successfully utilized to investigate precipitation microphysics and improve radar quantitative precipitation estimation(QPE).The recent progress in dual-pol radar research and applications in China is summarized in four aspects.Firstly,the characteristics of several representative dual-pol radars are reviewed.Various approaches have been developed for radar data quality control,including calibration,attenuation correction,calculation of specific differential phase shift,and identification and removal of non-meteorological echoes.Using dual-pol radar measurements,the microphysical characteristics derived from raindrop size distribution retrieval,hydrometeor classification,and QPE is better understood in China.The limited number of studies in China that have sought to use dual-pol radar data to validate the microphysical parameterization and initialization of numerical models and assimilate dual-pol data into numerical models are summarized.The challenges of applying dual-pol data in numerical models and emerging technologies that may make significant impacts on the field of radar meteorology are discussed. | Kun ZHAO Hao HUANG Mingjun WANG Wen-Chau LEE Gang CHEN Long WEN Jing WEN Guifu ZHANG Ming XUE Zhengwei YANG Liping LIU Chong WU Zhiqun HU Sheng CHEN | 2019 | Advances in Atmospheric Sciences2019，36，9： | 12 |
| 10 | The enhanced X-ray Timing and Polarimetry mission—eXTP显示文摘In this paper we present the enhanced X-ray Timing and Polarimetry mission—eXTP. eXTP is a space science mission designed to study fundamental physics under extreme conditions of density, gravity and magnetism. The mission aims at determining the equation of state of matter at supra-nuclear density, measuring effects of QED, and understanding the dynamics of matter in strong-field gravity. In addition to investigating fundamental physics, eXTP will be a very powerful observatory for astrophysics that will provide observations of unprecedented quality on a variety of galactic and extragalactic objects. In particular, its wide field monitoring capabilities will be highly instrumental to detect the electro-magnetic counterparts of gravitational wave sources.The paper provides a detailed description of:(1) the technological and technical aspects, and the expected performance of the instruments of the scientific payload;(2) the elements and functions of the mission, from the spacecraft to the ground segment. | ShuangNan Zhang Andrea Santangelo Marco Feroci YuPeng Xu FangJun Lu Yong Chen Hua Feng Shu Zhang Sφren Brandt Margarita Hernanz Luca Baldini Enrico Bozzo Riccardo Campana Alessandra De Rosa YongWei Dong Yuri Evangelista Vladimir Karas Norbert Meidinger Aline Meuris Kirpal Nandra Teng Pan Giovanni Pareschi Piotr Orleanski QiuShi Huang Stephane Schanne Giorgia Sironi Daniele Spiga Jiri Svoboda Gianpiero Tagliaferri Christoph Tenzer Andrea Vacchi Silvia Zane Dave Walton ZhanShan Wang Berend Winter Xin Wu Jean J.M.in't Zand Mahdi Ahangarianabhari Giovanni Ambrosi Filippo Ambrosino Marco Barbera Stefano Basso Jörg Bayer Ronaldo Bellazzini Pierluigi Bellutti Bruna Bertucci Giuseppe Bertuccio Giacomo Borghi XueLei Cao Franck Cadoux Francesco Ceraudo TianXiang Chen Yu Peng Chen Jerome Chevenez Marta Civitani Wei Cui WeiWei Cui Thomas Dauser Ettore Del Monte Sergio Di Cosimo Sebastian Diebold Victor Doroshenko Michal Dovciak YuanYuan Du Lorenzo Ducci QingMei Fan Yannick Favre Fabio Fuschino JoséLuis Ga'lvez Min Gao MingYu Ge Olivier Gevin Marco Grassi QuanYing Gu YuDong Gu DaWei Han Bin Hong Wei Hu Long Ji ShuMei Jia WeiChun Jiang Thomas Kennedy Ingo Kreykenbohm Irfan Kuvvetli Claudio Labanti Luca Latronico Gang Li MaoShun Li Xian Li Wei Li ZhengWei Li Olivier Limousin HongWei Liu XiaoJing Liu Bo Lu Tao Luo Daniele Macera Piero Malcovati Adrian Martindale Malgorzata Michalska Bin Meng Massimo Minuti Alfredo Morbidini Fabio Muleri Stephane Paltani Emanuele Perinati Antonino Picciotto Claudio Piemonte JinLu Qu Alexandre Rachevski Irina Rashevskaya Jerome Rodriguez Thomas Schanz ZhengXiang Shen LiZhi Sheng JiangBo Song LiMing Song Carmelo Sgro Liang Sun Ying Tan Phil Uttley Bo Wang DianLong Wang GuoFeng Wang Juan Wang LangPing Wang YuSa Wang Anna L.Watts XiangYang Wen Jörn Wilms ShaoLin Xiong JiaWei Yang Sheng Yang YanJi Yang Nian Yu WenDa Zhang Gianluigi Zampa Nicola Zampa Andrzej A.Zdziarski AiMei Zhang ChengMo Zhang Fan Zhang Long Zhang Tong Zhang Yi Zhang XiaoLi Zhang ZiLiang Zhang BaoSheng Zhao ShiJie Zheng Yu Peng Zhou Nicola Zorzi J.Frans Zwart | 2019 | Science China(Physics,Mechanics & Astronomy)2019，62，2： | 11 |
| 11 | Entrance Effect on Load Capacity of Orifice Compensated Aerostatic Bearing with Feeding Pocket显示文摘The current research of the aerostatic thrust bearing mainly focuses on the porous material bearing and inherent compensated air bearing, which aims at obtaining small physical dimension and large load capacity. Although porous material bearing appears larger load capacity, materials anisotropy itself and void content distortion caused in heat-treating, and machining processes add greater complexity to internal flow transfer process. Inherent compensated air bearing has the advantages of simple structure and good stability, but its load capacity and static stiffness is not worth somewhat. In this paper, based on hydrostatic lubrication theory, finite volume method is presented for taking entrance effects into account in computing pressure distribution, load capacity and mass flow rates of circular aerostatic thrust bearings. Technical analysis, numerical simulations and laboratory demonstration tests of influence of pocket diameter and pocket depth on loading capacity of aerostatic thrust bearing are carried out on simple orifice compensated air bearings with feeding pockets. The static parameters, such as air consumption and pressure distributions, are measured as a function of supply pressure and air gap height for several different orifices and pockets size. Entrance effects are described in term of typical throttling types, and the effect of pocket diameter and pocket depth on load capacity is systematically described respectively. The proposed research results uncover the causation of throttling action of the orifice compensated air bearing with feed pocket and further develop and improve the design theory of air bearing. | LONG Wei BAO Gang | 2010 | Chinese Journal of Mechanical Engineering2010，23，4： | 11 |
| 12 | Proportion of Uterine Malignant Tumors in Patients with Laparoscopic Myomectomy: A National Multicenter Study in China显示文摘 | Hua Yang Xiao-Chuan Li Chen Yao Jing-He Lang Hang-Mei Jin Ming-Rong Xi Gang Wang Lu-Wen Wang Min Hao Yan Ding Jie Chen Jian-Qing Zhang Lu Han Cheng-Xiu Guo Xiang Xue Yan Li Jian-Hua Zheng Man-Hua Cui Huai-Fang Li Guang-Shi Tao Long Chen Su-Min Wang An-Wei LU Ze-Hua Huang Qing Liu Ya-Li Zhuang Xiang-Hua Huang Gen-Hai Zhu Ou-Ping Huang Li-Na Hu Mu-Jun Li Hong-Lin Zhou Jing-Hui Song Lan Zhu | 2017 | Chinese Medical Journal2017，，22： | 10 |
| 13 | Pre-impact trajectory planning for minimizing base attitude disturbance in space manipulator systems for a capture task显示文摘Aimed at capture task for a free-floating space manipulator, a scheme of pre-impact trajectory planning for minimizing base attitude disturbance caused by impact is proposed in this paper.Firstly, base attitude disturbance is established as a function of joint angles, collision direction and relative velocity between robotic hand and the target.Secondly, on the premise of keeping correct capture pose, a novel optimization factor in null space is designed to minimize base attitude disturbance and ensure that the joint angles do not exceed their limits simultaneously.After reaching the balance state, a desired configuration is achieved at the contact point.Thereafter, particle swarm optimization(PSO) algorithm is employed to solve the pre-impact trajectory planning from its initial configuration to the desired configuration to achieve the minimized base attitude disturbance caused by impact and the correct capture pose simultaneously.Finally, the proposed method is applied to a 7-dof free-floating space manipulator and the simulation results verify the effectiveness. | Zhang Long Jia Qingxuan Chen Gang Sun Hanxu | 2015 | Chinese Journal of Aeronautics2015，28，4： | 9 |
| 14 | Detumbling strategy based on friction control of dual-arm space robot for capturing tumbling target显示文摘The rotational motion of a tumbling target brings great challenges to space robot on successfully capturing the tumbling target.Therefore,it is necessary to reduce the target's rotation to a rate at which capture can be accomplished by the space robot.In this paper,a detumbling strategy based on friction control of dual-arm space robot for capturing tumbling target is proposed.This strategy can reduce the target's rotational velocity while maintaining base attitude stability through the establishment of the rotation attenuation controller and base attitude adjustment controller.The rotation attenuation controller adopts the multi-space hybrid impedance control method to control the friction precisely.The base attitude adjustment controller applies the dual-arm extended Jacobian matrix to stabilize the base attitude.The main contributions of this paper are as follows:(1)The compliant control method is adopted to achieve a precise friction control,which can reduce the target angular velocity steadily;(2)The dual-arm extended Jacobian matrix is applied to stabilize the base attitude without affecting the target capture task;(3)The detumbling strategy of dualarm space robot is designed considering base attitude stabilization,realizing coordinated planning of the base attitude and the arms.The strategy is verified by a dual-arm space robot with two 7-DOF(degrees of freedom)arms.Simulation results show that,target with a rotation velocity of 20(°)/s can be effectively controlled to stop within 30 s,and the final deflection of the base attitude is less than 0.15°without affecting the target capture task,verifying the correctness and effectiveness of the strategy.Except to the tumbling target capture task,the control strategy can also be applied to other typical on-orbit operation tasks such as space debris removal and spacecraft maintenance. | Gang CHEN Yuqi WANG Yifan WANG Ji LIANG Long ZHANG Guangtang PAN | 2020 | Chinese Journal of Aeronautics2020，33，3： | 9 |
| 15 | Effects of yeast and yeast cell wall polysaccharides supplementation on beef cattle growth performance, rumen microbial populations and lipopolysaccharides production显示文摘This experiment was conducted to investigate the effects of live yeast and yeast cell wall polysaccharides on growth performance,rumen function and plasma lipopolysaccharides(LPS) content and immunity parameters of beef cattle.Forty Qinchuan cattle were randomly assigned to one of four treatments with 10 replicates in each treatment.The dietary treatments were: control diet(CTR),CTR supplemented with 1 g live yeast(2×10^10 live cell g^-1 per cattle per day(YST1),CTR supplemented with 2 g live yeast per cattle per day(YST2) and CTR supplemented with 20 g of yeast cell wall polysaccharides(30.0%≤β-glucan≤35.0%,and 28.0%≤mannanoligosaccharide≤32.0%) per cattle per day(YCW).The average daily gain was higher(P=0.023) and feed conversion ratio was lower(P=0.042) for the YST2 than the CTR.The digestibility of neutral detergent fiber(P=0.039) and acid detergent fiber(P=0.016) were higher in yeast supplemented groups.The acetic acid:propionic acid of the YST2 was lower compared with the CTR(P=0.033).Plasma LPS(P=0.032),acute phase protein haptoglobin(P=0.033),plasma amyloid A(P=0.015) and histamine(P=0.038) were lower in the YST2 compared with the CTR.The copies of fibrolytic microbial populations such as Fibrobacter succinogenes S85,Ruminococcus albus 7 and Ruminococcus flavefaciens FD-1 of the YST2 were higher(P<0.001),while the copies of typical lactate producing bacteria Streptococcus bovis JB1 was lower(P<0.001) compared with the CTR.Little differences were observed between the CTR,YST1 and YCW in growth performance,ruminal fermentation characteristics,microbial populations,immunity indices and total tract nutrient digestibility.It is concluded that the YST2 could promote fibrolytic microbial populations,decrease starch-utilizing bacteria,reduce LPS production in the rumen and LPS absorption into plasma and decrease inflammatory parameters,which can lead to an improvement in growth performance in beef cattle. | PENG Quan-hui CHENG Long KANG Kun Tian Gang Mohammad Al-Mamun XUE Bai WANG Li-zhi ZOU Hua-wei Mathew Gitau Gicheha WANG Zhi-sheng | 2020 | Journal of Integrative Agriculture2020，19，3： | 9 |
| 16 | Protective Effects of Quercetin on Cadmium-induced Cytotoxicity in Primary Cultures of Rat Proximal Tubular Cells显示文摘Objective To investigate the protective effects of quercetin on cadmium-induced cytotoxicity in primary cultures of rat proximal tubular(rPT)cells.Methods Primary cultures of rPT cells undergoing exponential growth were incubated with 1.0 μg/mL quercetin and/or cadmium(2.5,5.0 μmol/L),in a serum-free medium at 37 ℃ at different time intervals.Commercial kits were used and flow cytometric analyses were performed on rPT cell cultures to assay apoptosis and oxidative stress.Results Exposure of rPT cells to cadmium acetate(2.5,5.0 μmol/L)induced a decrease in cell viability,caused an increase in apoptotic rate and apoptotic morphological changes.Simultaneously,elevation of intracellular reactive oxygen species,malondialdehyde and calcium levels,depletion of mitochondrial membrane potential and intracellular glutathione,and inhibition of Na +,K +-ATPase,Ca 2+-ATPase,glutathione peroxidase(GSH-Px),catalase(CAT),and superoxide dismutase(SOD)activities were revealed during the cadmium exposure of rPT cells.However,simultaneous supplementation with 1 μg/mL quercetin protected rPT cells against cadmium-induced cytotoxicity through inhibiting apoptosis,attenuating lipid peroxidation,renewing mitochondrial function and elevating the intracellular antioxidants(non-enzymatic and enzymic)levels.Conclusion The present study has suggested that quercetin,as a widely distributed dietary antioxidant,contributes potentially to prevent cadmium-induced cytotoxicity in rPT cells. | WANG Lin LIN Shu Qian HE Yuan Long LIU Gang WANG Zhen Yong | 2013 | Biomedical and Environmental Sciences2013，26，4： | 8 |
| 17 | Estimation of reproduction numbers of COVID-19 in typical countries and epidemic trends under different prevention and control scenarios显示文摘The coronavirus disease 2019(COVID-19)has become a life-threatening pandemic.The epidemic trends in different countries vary considerably due to different policy-making and resources mobilization.We calculated basic reproduction number(R0)and the time-varying estimate of the effective reproductive number(Rt)of COVID-19 by using the maximum likelihood method and the sequential Bayesian method,respectively.European and North American countries possessed higher (R0)and unsteady Rt fluctuations,whereas some heavily affected Asian countries showed relatively low (R0)and declining Rt now.The numbers of patients in Africa and Latin America are still low,but the potential risk of huge outbreaks cannot be ignored.Three scenarios were then simulated,generating distinct outcomes by using SEIR(susceptible,exposed,infectious,and removed)model.First,evidence-based prompt responses yield lower transmission rate followed by decreasing Rt.Second,implementation of effective control policies at a relatively late stage,in spite of huge casualties at early phase,can still achieve containment and mitigation.Third,wisely taking advantage of the time-window for developing countries in Africa and Latin America to adopt adequate measures can save more people’s life.Our mathematical modeling provides evidence for international communities to develop sound design of containment and mitigation policies for COVID-19. | Chen Xu Yinqiao Dong Xiaoyue Yu Huwen Wang Lhakpa Tsamlag Shuxian Zhang Ruijie Chang Zezhou Wang Yuelin Yu Rusi Long Ying Wang Gang Xu Tian Shen Suping Wang Xinxin Zhang Hui Wang Yong Cai | 2020 | Frontiers of Medicine2020，14，5： | 8 |
| 18 | Establishment and evaluation of the system of extracorporeal liver perfusion in pigs显示文摘BACKGROUND; In recent years, extracorporeal liver per- fusion (ECLP) has been regarded as a treatment of acute liver failure ( ALF ); but the system of ECLP has many problems. The purpose of this experiment was to detect the factors affecting the system of ECLP and to establish a sta- ble and effective system of ECLP. METHODS; Livers were harvested from health pigs, ac- cording to the different styles of perfusion and oxygena- tion, which were randomly divided into 3 groups. The liv- ers in group A (n =4) were subjected to single portal vein perfusion, oxygenating perfusion blood; the livers in group B (n =4) to dual ( portal vein and hepatic artery) vessel perfusion, oxygenating blood, together; and the livers in group C ( n = 4) to dual (portal vein and hepatic artery) vessel perfusion, but oxygenating blood, separately. The perfusion time, the data of bile production, and hemody- namic parameters of extracorporeal livers in each group were tested. The histological examination of liver tissues from each group was performed at the end of perfusion. RESULTS: The perfusion time of the liver in group A is significantly shorter than in groups B and C (P<0.05). At 1 , 3 , 6 hours after perfusion, the data of bile production and hemodynamic parameters of livers in group A were sta- tistically different from those of livers in groups B and C (P<0.05). At 1, 3, 6 hours after perfusion, the data of group B were not statistically different from those of livers in group C (P>0.05). But at 12 hours after perfusion, the data of group B were statistically different from those of liv- ers in group C (P<0.01). CONCLUSION: The system of ECLP, which is performed by dual ( portal vein and hepatic artery) vessel perfusionand oxygenating blood separately, is more stable and effec- tive to keep the function of extracorporeal liver. | Jin Gong, Xi-Mo Wang, Gang Long, Zhong-Tao Guo, Tao Jiang and Shi Chen Wuhan, ChinaInstitute of Organ Transplantation, Tongji Hospital, Tongji Medical College , Huazhong University of Science and Technology, Wuhan 430030, China Department of Surgery, Tianjin People’s Hospital, Tianjin 300120, China | 2005 | Hepatobiliary & Pancreatic Diseases International2005，4，1： | 7 |
| 19 | Primary evidence for involvement of IP_(3) in heat-shock signal transduction in Arabidopsis显示文摘热吃惊(HS ) 表明的在 transducing 的肌醇 1,4,5-trisphosphate (IP (3 )) 的角色在 Arabidopsis 被检验。整个植物的 IP (3 ) 水平在 37 度 C 在 HS 的 1 min 以内增加了。在 HS 的 3 min 以后, IP (3 ) 水平到达了最大值 2.5 褶层增加。用有 AtHsp18.2 promoter-beta-glucuronidase ( GUS )的转基因的 Arabidopsis 植物熔化基因, GUS 活动的水平在 non-HS 和 HS 温度由将关入笼中的 IP ( 3 )的增加是起来调整的并且由 phospholipase C ( PLC )禁止者是下面调整的,这被发现{1-[6-(( 17beta-3-Methoxyestra-1,3,5 ( 10 ) -trien-17-yl)amino)hexyl]-2,5-pyrro lidinedione }( U-73122 )。细胞内部免费的钙离子集中([Ca (2+)](i)) 在表示 apoaequorin 的暂停有教养的 Arabidopsis 房间在 37 度 C 在 HS 期间增加了。有 U-73122 的处理阻止了增加[Ca (2+)](i) 到某程度。上面的结果在更高的植物在 HS 信号 transduction 为 IP (3 ) 的可能的参与提供了主要证据。 | Hong Tao Liu Fei Gao Shu Juan Cui Jin Long Han Da Ye Sun Ren Gang Zhou | 2006 | Cell Research2006，16，4： | 7 |
| 20 | Methane aromatization in the absence of oxygen over extruded and molded MoO_3/ZSM-5 catalysts:Influences of binder and molding method显示文摘The influences of binder and molding method on the catalytic performance of methane aromatization in the absence of O2 over MoO3/ZSM-5 catalysts were investigated.SEM,NH3-TPD,FT-IR of adsorbed pyridine,N2 adsorption-desorption,cyclohexane adsorption and XPS were employed to characterize the physical and chemical properties of the catalysts.It was found that SiO2 was a suitable binder for the catalyst due to its appropriate weak acidity.The laminar catalyst comprising of an inert spherical core and a MoO3/ZSM-5 laminar shell with 0.1 0.2 mm in thickness showed a better catalytic performance than the extruded catalyst.The improved activity of the laminar catalyst could be attributed to the easy carbonization of Mo species and the quick removal of reaction products from the catalyst surface. | Dongmei Ren Xiangsheng Wang Gang Li Xiaojing Cheng Huayun Long Lidong Chen | 2010 | Journal of Natural Gas Chemistry2010，19，6： | 7 |
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