|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Anti-tumor necrosis factor α therapy associates to type 17 helper T lymphocytes immunological shift and significant microbial changes in dextran sodium sulphate colitis显示文摘BACKGROUND Anti-tumor necrosis factor α(TNFα) represents the best therapeutic option to induce mucosal healing and clinical remission in patients with moderate-severe ulcerative colitis. On the other side gut microbiota plays a crucial role in pathogenesis of ulcerative colitis but few information exists on how microbiota changes following anti-TNFα therapy and on microbiota role in mucosal healing.AIM To elucidate whether gut microbiota and immune system changes appear following anti TNFα therapy during dextran sulfate sodium(DSS) colitis.METHODS Eighty C57 BL/6 mice were divided into four groups: 'No DSS', 'No DSS + antiTNFα', 'DSS' and 'DSS + anti-TNFα'. 'DSS' and 'DSS + anti-TNFα' were treated for 5 d with 3% DSS. At day 3, mice whithin 'No DSS+anti-TNFα' and'DSS+anti-TNFα' group received 5 mg/kg of an anti-TNFα agent. Forty mice were sacrificed at day 5, forty at day 12, after one week of recovery post DSS. The severity of colitis was assessed by a clinical score(Disease Activity Index), colon length and histology. Bacteria such as Bacteroides, Clostridiaceae, Enterococcaceae and Fecalibacterium prausnitzii(F. prausnitzii) were evaluated by quantitative PCR.Type 1 helper T lymphocytes(Th1), type 17 helper T lymphocytes(Th17) and CD4+ regulatory T lymphocytes(Treg) distributions in the mesenteric lymph node(MLN) were studied by flow cytometry.RESULTS Bacteria associated with a healthy state(i.e., such as Bacteroides, Clostridiaceae and F. prausnitzii) decreased during colitis and increased in course of anti-TNFαtreatment. Conversely, microorganisms belonging to Enterococcaceae genera,which are linked to inflammatory processes, showed an opposite trend.Furthermore, in colitic mice treated with anti-TNFα microbial changes were associated with an initial increase(day 5 of the colitis) in Treg cells and a consequent decrease(day 12 post DSS) in Th1 and Th17 frequency cells. Healthy mice treated with anti-TNFα showed the same histological, microbial and immune features of untreated colitic mice. 'No DSS + anti-TNFα' group showed a lymphomononuclear infiltrate both at 5 th and 12 th d at hematoxylin and eosin staining, an increase of in Th1 and Th17 frequency at day 12, an increase of Enterococcaceae at day 5, a decrease of Bacteroides and Clostridiaceae at day 12.CONCLUSION Anti-TNFα treatment in experimental model of colitis improves disease activity but it is associated to an increase in Th17 pathway together with gut microbiota alteration. | Valentina Petito Cristina Graziani Loris R Lopetuso Marco Fossati Alessandra Battaglia Vincenzo Arena Domenico Scannone Gianluca Quaranta Andrea Quagliariello Federica Del Chierico Lorenza Putignani Luca Masucci Maurizio Sanguinetti Alessandro Sgambato Antonio Gasbarrini Franco Scaldaferri | 2019 | World Journal of Gastroenterology2019，25，12： | 2 |
| 2 | Randomized study of adjuvant chemotherapy for completly resected stage Ⅰ,Ⅱ,or Ⅲ A non-smallcell Lung cancer显示文摘 | Scagliotti GV Fossati R Torri V | 2003 | J Natl Cancer Inst2003，95，19： | 1 |
| 3 | Cognitive control andbrain resources in major depression : an fMRI study using the n-back task显示文摘 | Harvey PO Fossati R Poehon JB | 2005 | Neuroimage2005，26，3： | 1 |
| 4 | Psyehologieal str- ess in nurses' relationships with H1V-infeeted patients:the risk of burmout syndrome显示文摘 | Visintini R Campanlni E Fossati A | 1996 | AIDS Care1996，8，2： | 1 |
| 5 | The lupus-prone BXSB strain:the Yaa gene model of systemic lupus erythematosus显示文摘 | Merino R Fossati L Izui S | 1992 | Springer Semin lmmunopathol1992，14，2： | 1 |
| 6 | Randomized study of adjuvant chemotherapy for completely resected stage Ⅰ,Ⅲ,or Ⅲ A non-small-cell lung cancer显示文摘 | Scagliotti GV Fossati R Torri V | 2003 | Natl Cancer Inst2003，95，19： | 1 |
| 7 | The Neural System that Bridges Reward and Cognition in Humans:an fMRI Study显示文摘 | Pochon JB Levy R Fossati P | 2002 | Proc Natl Acad Sci USA2002，99，8： | 1 |
| 8 | Use of simulated intestirtal fluid for Caco - 2 permeability assay of lipophilic drugs 显示文摘 | Fossati L Deehaume R Hardillier E | 2008 | International Journal of Pharmaceutics2008，360，12： | 1 |
| 9 | Psychological stress in nurses' relationships with HIVinfected patients:the risk of burnout syndrome显示文摘 | VISINTINI R CAMPANINI E FOSSATI A | 1996 | AIDS Care1996，8，2： | 1 |
| 10 | Randomized trial of neoadjuvant chemotherapy comparing paclitaxel, ifosfamide, and cisplatin with ifosfamide and cisplatin followed by radical surgery in patients with locally advanced squamous cell cervical carcinoma: the SNAP01 ( Studio Neo-Adjuvante Portio ) Italian Collaborative Study 显示文摘 | Buda A Fossati R Colombo N | 2005 | J Clin Oncol2005，23，18： | 1 |
| 11 | Randomized study of adjuvant chemotherapy for completely resected stage Ⅰ,Ⅱ,or Ⅲ A non-small cell lung cancer显示文摘 | Scagliotti GV Fossati R Torri V | 2003 | J Natl Cancer Inst2003，95，： | 1 |
| 12 | Psychological stress in nurses'relationships with HIV 2 in fected patients:The risk of burn- out syndrome 显示文摘 | Visintini R Camapanini E Fossati A | 1996 | AIDS Care1996，8，2： | 1 |
| 13 | Psychologicalstress in nurses'relationshps with HIV 2in fected patients: the risk of burnout syndrome显示文摘 | Visintini R Campanini E Fossati A | 1996 | Care1996，8，2： | 1 |
| 14 | Randomized trial of neoadjuvant chemotherapy comparing paclitaxel, ifosfamide, and cisplatin with ifosfamide and cisplatin followed by radical surgery in patients with locally advanced squamous cell cervical carcinoma: the SNAP01 (Studio Neo-Adjuvante Portio) Italian Collaborative Study 显示文摘 | Buda A Fossati R Colombo N | 2005 | J Clin Oncol2005，23，18： | 1 |
| 15 | Randomized study of adjuvant chemotherapy for completely resected stage I , Ⅱ or Ⅲ A non-small cell lung cancer 显示文摘 | Scaglitotti GV Fossati R Torri V | 2003 | J Natl Cancer Inst2003，95，： | 1 |
| 16 | Executive functions and updating of the contents of working memory in unipolar depression显示文摘 | Harvey P.O Le Bastard G Pochon J.B Levy R Allilaire J.F Dubois B & Fossati P | | 0，，06： | 1 |
| 17 | Psychological stress in nurses'relationshops with HIV infected patients:the risk of burnout syndrome显示文摘 | Visintini R Campanini E Fossati A | 1996 | AIDS Care1996，8，2： | 1 |
| 18 | Randomized study of adjuvant chemotherapy for completely resected stage I , Ⅱ , or II1 A non-small-cell lung cancer显示文摘 | Scagliotti GV Fossati R Toni V | 2003 | J Natl Cancer Inst2003，95，19： | 1 |
| 19 | Psychological stress in nurses' relationships with HIV - infected patients: The risk of burnout syndrome 显示文摘 | Visinitini R Campanini E Fossati A | 1996 | AIDS Care1996，，8： | 1 |
| 20 | Pediatric medullobiastoma: toxicity of current treatment and potential role of protontherapy 显示文摘 | Fossati P Ricardi U Orecchia R | 2009 | Cancer Treat Rev2009，351，： | 1 |
您所在的机构暂未开通此部分的功能，请联系管理员