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1A Comparison Between Northern Blotting and Quantitative Real-Time PCR as a Means of Detecting the Nutritional Regulation of Genes Expressed in Roots of Arabidopsis thaliana显示文摘Quantitative real-time PCR (qRT-PCR) has become a routine and robust technique for measuring the expression of genes of interest, validating microarray experiments and monitoring biomarkers. However, concerns have been raised over the accuracy of qRT-PCR in China as well as in the rest of the world. We have previously used qRT-PCR to study the response of ANR1 and other root-expressed MADS-box genes to fluctuations in the supply of nitrate, phosphate and sulphate under hydroponic growth conditions. In this study, we have used both Northern blotting and qRT-PCR analyses to confirm the nutritional regulation of MADS-box genes in Arabidopsis thaliana and test whether both technologies produce the same results. The information obtained indicated that the qRT-PCR results are consistent with those obtained by Northern blotting hybridization for all the tested root-expressed MADS-box genes, in response to different nitrate, phosphate and sulphate growth conditions. Furthermore, our novel results showed that the expressions of AGL12, AGL18, and AGL19 were all down regulated in response to S and P re-supply in both qRT-PCR and Northern blotting analyses.GAN Yin-bo ZHOU Zhong-jing AN Li-jun BAO Sheng-jie Brian G Forde 2011Agricultural Sciences in China2011,10,3:4
2高危心血管病老年人血压变异性的相关因素显示文摘诊问血压变异性是心血管病的独立预测因素。该研究探讨老年人血压变异的相关因素。方法:在普伐他汀干预高危老年人的前瞻性研究( pravastatin inelderly individuals at risk of vascular disease, PROS-PER)中,分析70~82岁有心血管病史或危险因素的男性和女性者3794人。Poortvliet RK Lloyd SM Ford I Sattar N de Craen AJ Wijsman LW Mooijaart SP Westendorp G Jukema JW de Ruijter W Gussekloo J Stott DJ 2015中华高血压杂志2015,23,10:3
3An arabidopsis MADs box gene that controls nutrient - induced changes in root architecture显示文摘Zhang H M and Forde B G 1998Science1998,279,:2
4Regulation of Arabidopsis root development by nitrateavailability显示文摘Zhang H M Forde B G 2000Journal of Experimental Botany2000,51,342:2
5Nitrate transporters in plants:Structure,function and regulation显示文摘FORDE B G 2000Biochim Biophys Acta2000,1465,:1
6The nutritional control of root development显示文摘Forde B G Lorenzo H 2001Plant Soil2001,232,:1
7The role of the surgeon in the evolution of flexible endoscopy显示文摘Morgenthal C B Richards W O Dunkin B J Forde K A Vitale G Lin E 2007Surg Endosc2007,21,6:1
8Munumbicins, wide-spectrum antibiotics produced by Streptomyces NRRL 30562, endophytic on Kennedia nigriscans 显示文摘Castillo U F Strobel G A Ford E J 2002Microbiology2002,148,9:1
9Munumbicins, wide spectrum antibiotics produced by Streptomyces endophytic on Kennedia nigriscans显示文摘CASTILLO U F STROBEL G A FORD E J 2002Microbiology2002,148,:1
10Cardiovascular and oral disease interactions: what is the evidence显示文摘Ford P J Yamazaki K Seymour G J 2007? Prim Dent Care2007,14,2:1
11Separation and analysis of the diastereomers and enantiomers of cypermethrin and related compounds显示文摘Edwards D P Ford M G 1997Journal of Chromatography A1997,777,2:1
12Acin: a 1, 3-di- hydroisobenzofuran from Pestalotiopsis microspora possessing an- tioxidant and antimycotic activities显示文摘Harper J K Ford E J Strobe1 G A 2003Tetrahedron2003,59,:1
13Neural processes associated with antisaccade task performance investigated with event- related fMRI显示文摘FORD K A GOLTZ H C BROWN M R G 2005Journal of Neurophysiology2005,94,1:1
14Nitrate and ammonium nutrition of plants:physiological and molecular perspectives显示文摘FORDE B G CLARKSON D T 1999Adv Bot Res1999,30,:1
15Munumbicins,Wide-spectrum Antibiotics Produced by Streptomyces NRRL 30562,Endophyfic on Kennedia nigfiscans显示文摘CASTILLO U F STROBEL G A FORD EJ 2002Microbiology2002,148,:1
16CHl-IIPS(Controlling Hypertension and I-Iypotension Immediately Post-Stroke)Pilot Trial: rationale and desigaa 显示文摘Potter J Robinson T Ford G 2005J Hypertens2005,23,:1
17Nature Biotechnology 显示文摘Forde G M 200523(9): 1059-10622005,23,9:1
18Independent associations of serum concentrations of 25-hydroxyvitamin D and parathyroid hormone with blood pressure among US adults显示文摘Zhao G Ford ES Li C 2010J Hypertens2010,28,9:1
19Canopy transpiration using time series analysis: A case study illustrating the effect of soil moisture deficit on Pinus taeda 显示文摘Ford C R Carol E G Robert J M 2005Journal of Agricultural and Forest Meteorology2005,130,:1
20Transient Ground Potential Rise in Gas Insulated Subs- tations - Experimental Studies 显示文摘FUJIMOTO N DICK E P BOGGS S A FORD G L 1982IEEE Trans on Power Apparatus and Systems1982,101,1:1
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