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| 1 | Structural and functional aspects of the liver and liver sinusoidal cells in relation to colon carcinoma metastasis显示文摘Nowadays, liver metastasis remains difficult to cure. When tumor cells escape and arrive in the liver sinusoids, they encounter the local defense mechanism specific to the liver. The sinusoidal cells have been widely described in physiologic conditions and in relation to metastasis during the past 30 years. This paper provides an 'overview' of how these cells function in health and in diseases such as liver metastasis. | Katrien Vekemans Filip Braet | 2005 | World Journal of Gastroenterology2005，11，33： | 4 |
| 2 | The hepatic sinusoidal endothelial lining and colorectal liver metastases显示文摘Colorectal cancer (CRC) is a common malignant disease and the severe nature of cases in men and women who develop colorectal cancer makes this an important socio-economic health issue. Major challenges such as understanding and modeling colorectal cancer pathways rely on our understanding of simple models such as outlined in this paper. We discuss that the development of novel standardized approaches of multidimensional (correlative) biomolecular microscopy methods facilitates the collection of (sub) cellular tissue information in the early onset of colorectal liver metastasis and that this approach will be crucial in designing new effective strategies for CRC treatment. The application of X-ray micro-computed tomography and its potential in correlative imaging of the liver vasculature will be discussed. | Filip Braet Keissuke Nagatsuma Masaya Saito Lilian Soon Eddie Wisse Tomokazu Matsuura | 2007 | World Journal of Gastroenterology2007，13，6： | 3 |
| 3 | Multifaceted nature of membrane microdomains in colorectal cancer显示文摘Membrane microdomains or lipid rafts are known to be highly dynamic and to act as selective signal transduction mediators that facilitate interactions between the cell's external and internal environments.Lipid rafts play an important mediating role in the biology of cancer:they have been found in almost all existing experimental cancer models,including colorectal cancer (CRC),and play key regulatory roles in cell migration,metastasis,cell survival and tumor progression.This paper explores the current state of knowledge in this field by highlighting some of the pioneering and recent lipid raft studies performed on different CRC cell lines and human tissue samples.From this literature review,it becomes clear that membrane microdomains appear to be implicated in all key intracellular signaling pathways for lipid metabolism,drug resistance,cell adhesion,cell death,cell proliferation and many other processes in CRC.All signal transduction pathways seem to originate directly from those peculiar lipid islands,thereby orchestrating the colon cancer cells' state and fate.As confirmed by recent animal and preclinical studies in different CRC models,continuing to unravel the structure and function of lipid rafts-including their associated complex signaling pathways-will likely bring us one step closer to better monitoring and treating of colon cancer patients. | Kristina A Jahn Filip Braet | 2011 | World Journal of Gastroenterology2011，17，6： | 2 |
| 4 | Reconstruction of liver organoid using a bioreactor显示文摘瞄准:用一个简历人工的肝为肝机关在试管内的重建开发有效技术。方法:我们以前报导一个光线流动的生物反应器(RFB ) 能提供一个三维的高密度的文化系统。我们目前重建了和老鼠把一根功能的人的肝细胞癌房间线(FLC-5 ) 用作 hepatocytes 的肝或硬鳞鱼使房间(秒) 线 M1 和老鼠使不朽的正弦曲线内皮不朽肝的星形的房间(HSC ) 在 RFB 作为非实质的的房间衬里 A7。二 x 10 (7 ) FLC-5 房间在 RFB 被孵化。在 5 d 以后, 2 x 10 (7 ) A7 房间以 10 的另一增加(7 ) 跟随的一种类似的方式被增加 M1 房间 5 d 以后。在三天灌注以后,有支持者房间的一些纤维素祷告被收获。最后潜伏期与支持者房间一起为 2 h 然后留下的纤维素祷告与 200 nmol/L swinholide A 包括了灌注从 RFB 被收获。房间形态学被传播电子显微镜学(TEM ) 和扫描电子显微镜学(SEM ) 观察。估计 hepatocyte 功能,我们在 RFB 单个类型的文化和 cocultures 与那些相比在单层文化由 FLC-5 为脲周期酶以及白朊合成比较了 mRNA 表示。结果:由传播电子显微镜学, FLC-5, M1,和 A7 在一个像肝的组织在与灌注方面的关系被安排。类似于胆汁 canaliculi 的结构在 FCL-5 房间之间被看见。扫描电子显微镜学在秒表面上表明了窗。当我们介绍了肌动朊绑定代理人 swinholide 时, vesiculo-vacuolar 细胞器(VVO ) 和窗的数字增加了 -- 在为 2h 的 RFB 的 A。关于肝功能,脲在编码 arginosuccinate 合成酶的 mRNAs 的媒介,和表达式被发现,当三种房间类型是在 RFB 的 cocultured 时,精氨酸酶增加了。然而,白朊合成减少了。结论:在 RFB 系统罐头的合作文化戏剧性地改变所有房间类型的结构和功能,包括 hepatocytes 的功能的特征。我们的系统用一个简历人工的肝为一个肝或硬鳞鱼的重建证明有效。 | Masaya Saito Tomokazu Matsuura Takahiro Masaki Tetsuro Suzuki Haruka Maehashi Keiko Shimizu Yoshiaki Hataba Tohru Iwahori Filip Braet | 2006 | World Journal of Gastroenterology2006，12，12： | 2 |
| 5 | Hydrodynamics based transfection in normal and fibrotic rats显示文摘AIM: Hydrodynamics based transfection (HBT), the injection of a large volume of naked plasmid DNA in a short time is a relatively simple, efficient and safe method for in vivo transfection of liver cells. Though used for quite some time, the mechanism of gene transfection has not yet been elucidated. METHODS: A luciferase encoding plasmid was injected using the hydrodynamics based procedure into normal and thioacetamide-induced fibrotic Sprague Dawley rats. Scanning and transmission electron microscopy images were taken. The consequence of a dual injection of Ringer solution and luciferase pDNA was followed. Halofuginone, an anti collagen type I inhibitor was used to reduce ECM load in fibrotic rats prior to the hydrodynamic injection. RESULTS: Large endothelial gaps formed as soon as 10’ following hydrodynamic injection; these gradually returned to normal 10 d post injection. Hydrodynamic administration of Ringer 10 or 30 m prior to moderate injection of plasmid did not result in efficient transfection suggesting that endothelial gaps by themselves are not sufficient for gene expression. Gene transfection following hydrodynamic injection in thioacetamide induced fibrotic rats was diminished coinciding with the level of fibrosis. Halofuginone, a specific collagen typeⅠ inhibitor, alleviated this effect. CONCLUSION: The hydrodynamic pressure formedfollowing HBT results in the formation of large endothelial gaps. These gaps, though important in the transfer of DNA molecules from the blood to the space of Disse are not enough to provide the appropriate conditions for hepatocyte transfection. Hydrodynamics based injection is applicable in fibrotic rats provided that ECM load is reduced. | Rita Yeikilis Shunit Gal Natalia Kopeiko Melia Paizi Mark Pines Filip Braet Gadi Spira | 2006 | World Journal of Gastroenterology2006，12，38： | 1 |
| 6 | Caveolae and caveolin-1 in reptilian liver显示文摘 | Joanna M. Biazik Kristina A. Jahn Filip Braet | 2011 | Micron2011，，6： | 1 |
| 7 | Influence of kavain on hepatic ultrastructure显示文摘AIM: To investigate whether the major kavalactone kavain imposes adverse effects on the liver ultrastructure and function by affecting vascular and microvascular architecture and altering hepatocellular morphology. METHODS: Kavain solution (10 μg/mL or 43.5 μmol/L) was perfused for 2 h in isolated rat livers. After standard fi xation and tissue preparation,the samples were examined by scanning electron microscopy (SEM),transmission electron microscopy (TEM),and light microscopy (LM). RESULTS: LM,SEM,and TEM examinations indicated kavain-treated rat livers (n = 4) displayed severe vascular and endothelial damage compared to control livers (n = 4). CONCLUSION: The data so far support the hypothesis that kavain induces adverse effects on liver; additional investigations with other kavalactones and their effects on liver are urgently needed. | Shuang Fu Emine Korkmaz Filip Braet Quan Ngo Iqbal Ramzan | 2008 | World Journal of Gastroenterology2008，14，4： | 1 |
| 8 | Fixation methods for electron microscopy of human and other liver显示文摘For an electron microscopic study of the liver,expertise and complicated,time-consuming processing of hepatic tissues and cells is needed.The interpretation of electron microscopy(EM) images requires knowledge of the liver fine structure and experience with the numerous artifacts in fixation,embedding,sectioning,contrast staining and microscopic imaging.Hence,the aim of this paper is to present a detailed summary of different methods for the preparation of hepatic cells and tissue,for the purpose of preserving long-standing expertise and to encourage new investigators and clinicians to include EM studies of liver cells and tissue in their projects. | Eddie Wisse Filip Braet Hans Duimel Celien Vreuls Ger Koek Steven WM Olde Damink Maartje AJ van den Broek Bart De Geest Cees HC Dejong Chise Tateno Peter Frederik | 2010 | World Journal of Gastroenterology2010，16，23： | 1 |
| 9 | Structural and functional aspects of liver sinusoidal endothelial cell fenestrae: a review 显示文摘 | Filip Braet Eddie Wisse | 2002 | Comparative Hepatology2002，1，： | 1 |
| 10 | Unlocking the ultrastructure of colorectal cancer cells in vitro using selective staining显示文摘AIM: To characterise differences between three widely used colorectal cancer cell lines using ultrastructural selective staining for glycogen to determine variation in metastatic properties. METHODS: Transmission electron microscopy was used in this investigation to help identify intracellular structures and morphological features which are precursors of tumor invasion. In addition to morphological markers, we used selective staining of glycogen as a marker for neoplastic cellular proliferation and determined whether levels of glycogen change between the three different cell lines. RESULTS: Ultrastructural analysis revealed morphological differences between the cell lines, as well as differentiation into two sub-populations within each cell line. Caco-2 cells contained large glycogen deposits as well as showing the most obvious morphological changes between the two sub-populations. SW480 cells also contained large glycogen stores as well as deep cellular protrusions when grown on porous filter membranes. HT-29 cells had trace amounts of glycogen stores with few cellular projections into the filter pores and no tight junction formation.CONCLUSION: Morphology indicative of metastatic properties coincided with larger glycogen deposits, providing strong evidence for the use of selective staining to determine the neoplastic properties of cells. | Joanna M Biazik Kristina A Jahn Filip Braet | 2010 | World Journal of Gastroenterology2010，16，22： | 0 |
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