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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | Biological therapy in inflammatory bowel diseases:Access in Central and Eastern Europe显示文摘Biological drugs opened up new horizons in the management of inflammatory bowel diseases(IBD).This study focuses on access to biological therapy in IBD patients across 9 selected Central and Eastern European(CEE)countries,namely Bulgaria,the Czech Republic,Estonia,Hungary,Latvia,Lithuania,Poland,Romania and Slovakia.Literature data on the epidemiology and disease burden of IBD in CEE countries was systematically reviewed.Moreover,we provide an estimation on prevalence of IBD as well as biological treatment rates.In all countries with the exception of Romania,lower biological treatment rates were observed in ulcerative colitis(UC)compared to Crohn’s disease despite the higher prevalence of UC.Great heterogeneity(up to 96-fold)was found in access to biologicals across the CEE countries.Poland,Bulgaria,Romania and the Baltic States are lagging behind Hungary,Slovakia and the Czech Republic in their access to biologicals.Variations of reimbursement policy may be one of the factors explaining the differences to a certain extent in Bulgaria,Latvia,Lithuania,and Poland,but association with other possible determinants(differences in prevalence and incidence,price of biologicals,total expenditure on health,geographical access,and cost-effectiveness results)was not proven.We assume,nevertheless,that healthdeterioration linked to IBD might be valued differently against other systemic inflammatory conditions in distinct countries and which may contribute to the immense diversity in the utilization of biological drugs for IBD.In conclusion,access to biologicals varies widely among CEE countries and this difference cannot be explained by epidemiological factors,drug prices or total health expenditure.Changes in reimbursement policy could contribute to better access to biologicals in some countries. | Fanni Rencz Márta Péntek Martin Bortlik Edyta Zagorowicz Tibor Hlavaty Andrzej Sliwczyński Mihai M Diculescu Limas Kupcinskas Krisztina B Gecse László Gulácsi Peter L Lakatos | 2015 | World Journal of Gastroenterology2015，21，6： | 4 |
| 3 | Determination of dopamine by flow - injection analysis coupled with luminol- hexacyanoferrate(Ⅲ) chemiluminescence detection 显示文摘 | Edyta N R Rosa B Anatol K | 2004 | J Pharma and Biomed Anal2004，36，1： | 1 |
| 4 | Application of multiplex FISH, CGH and MSSCP techniques for cytogenetic and molecular a- nalysis of transitional cell carcinoma ( TCC ) cells in voided urine specimens显示文摘 | Maria C Aleksandra B K Edyta B | 2006 | J Appl Genet2006，47，3： | 1 |
| 5 | Determination of dopamine by flow-injection analysis coupled with luminol-hexacyanoferrate(III) chemiluminescence detection显示文摘 | EDYTA N ROSA B R ANATOL K | | 0，，01： | 1 |
| 6 | Determination of dopamine by flow-injection analysis coupled with luminolhexacyanoferrate(Ⅲ) chemiluminescence detection显示文摘 | EDYTA N ROSA B R ANATOL K | 2004 | Journal of Pharmaceutical and Biomedical Analysis2004，36，1： | 1 |
| 7 | Microbial transformations of flavanone by Aspergillus niger and Peni- cillium chermesinum cultures 显示文摘 | Edyta K S Jadwiga D G Agata B | 2008 | Journal of Molecular Catalysis B : Enzymatic2008，，5253： | 1 |
| 8 | Determination of dopamine by flow-injection analysis coupled with luminol-hexacyano- ferrate(III) chemiluminescence detection显示文摘 | Edyta N Rosa R B Anatol K | 2004 | J Pharm Bi- omed Anal2004，36，1： | 1 |
| 9 | The role of cytokines in the pathogenesis of rheuma- toid arthritis - Practical and potential application of cytokines as bio- markers and targets of personalized therapy 显示文摘 | Edyta B Ewa B | 2015 | Cytokine2015，8，： | 1 |
| 10 | Application of multiplex FISH, CGH and MSSCP techniques for cytogenetic and molecular analysis of transitional cell carcinoma (TCC) cells in voided urine specimens显示文摘 | Maria C Aleksandra B K Edyta B | 2006 | J Appl Genet2006，47，3： | 1 |
| 11 | Determination of dopamine by flow - injection analysis coupled with luminol -hexacyanoferrate(III) chemiluminescence detection 显示文摘 | Edyta N R Rosa B Anatol K | 2004 | J Pharma and Biomed Anal2004，36，1： | 1 |
| 12 | Notchl as a potential therapeutic target in cutaneous T-cell Lymphoma 显示文摘 | Maria RK Lise MR Edyta B | 2010 | Blood2010，116，14： | 1 |
| 13 | Application of multiplex FISH, CGH and MSSCP techniques for cytogenetic and molecular analysis of transitional cell carcinoma (TCC) cells 显示文摘 | Maria C Aleksandra B K Edyta B | 2006 | J Appl Genet2006，47，3： | 1 |
| 14 | Prevalence of human papillomavirus DNA in difference histological subtypes of cervical adenocarcinoma显示文摘 | Edyta C Pirog Kleter B | 2000 | Am J Cancer2000，157，4： | 1 |
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