| 1 | Nondestructive and Fast Vibration Phenotyping of Plants显示文摘The frequencies of free oscillations of plants,or plant parts,depend on their geometries,stiffnesses,and masses.Besides direct biomechanical interest,free frequencies also provide insights into plant properties that can usually only bemeasured destructively or with low-throughput techniques(e.g.,change inmass,tissue density,or stiffness over development or with stresses).We propose here a new high-throughput method based on the noncontact measurements of the free frequencies of the standing plant.The plant is excited by short air pulses(typically 100ms).The resulting motion is recorded by a high speed video camera(100 fps)and processed using fast space and time correlation algorithms.In less than aminute the mechanical behavior of the plant is tested over several directions.The performance and versatility of this method has been tested in three contrasted species:tobacco(Nicotiana benthamian),wheat(Triticum aestivum L.),and poplar(Populus sp.),for a total ofmore than 4000 data points.In tobaccowe show that water stress decreased the free frequency by 15%.In wheat we could detect variations of less than 1 g in the mass of spikes.In poplar we could measure frequencies of both the whole stem and leaves.The work provides insight into new potential directions for development of phenotyping. | E.de Langre O.Penalver P.Hémon J.-M.Frachisse M.-B.Bogeat-Triboulot B.Niez E.Badel B.Moulia | 2019 | Plant Phenomics2019，1，1： | 1 |
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