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| 1 | Endostatin enhances antitumor effect of tumor antigen-pulsed dendritic cell therapy in mouse xenograft model of lung carcinoma显示文摘Objective: To investigate the antitumor effect of endostatin combined with tumor antigen-pulsed dendritic cell(DC)-T cell therapy on lung cancer.Methods: Transplanted Lewis lung cancer(LLC) models of C57BL/6 mice were established by subcutaneous injection of LLC cells in left extremity axillary. Tumor antigen-pulsed DC-T cells from spleen cells and bone of mice were cultured in vitro. Tumor-bearing mice were randomly divided into three groups, including DCT+endostatin group, DC-T group, and phosphate-buffered saline(PBS) control group. Microvessel density(MVD) of tumor tissue in tumor-bearing mice was determined by immunohistochemistry(IHC). The expressions of vascular endothelial growth factor(VEGF) and hypoxia-inducible factor-1α(HIF-1α) were determined by Western blotting and IHC staining. The proportions of CD8+ T cells, mature dendritic cells(m DC), tumor-associated macrophages [TAM(M1/M2)], and myeloid-derived suppressor cells(MDSC) in suspended cells of tumor tissue were determined by flow cytometry. The expressions of interleukin(IL)-6, IL-10, IL-17, transforming growth factor-β(TGF-β) and interferon-γ(IFN-γ) in suspended cells of tumor tissue were detected by enzyme-linked immune sorbent assay(ELISA).Results: DC-T cells combined with endostatin remarkably suppressed tumor growth. MVD of mice in DCT+endostatin group was significantly lower than that of the control group and DC-T monotherapy group. The expressions of VEGF, IL-6 and IL-17 in tumors were markedly decreased, but IFN-γ and HIF-1α increased after treating with DC-T cells combined with endostatin, compared to control group and DC-T group. In the DCT+endostatin group, the proportions of MDSC and TAM(M2 type) were significantly decreased, m DC and TAM(M1 type) were up-regulated, and CD8+ T cells were recruited to infiltrate tumors, in contrast to PBS control and DC-T monotherapy. DC-T cells combined with endostatin potently reduced the expressions of IL-6, IL-10, TGF-β and IL-17 in tumor tissue, and enhanced the expression of IFN-γ.Conclusions: The study indicated the synergic antitumor effects between endostatin and tumor antigen-pulsed DC-T cells, which may be a prospective therapy strategy to achieve potent antitumor effects on lung cancer. | ring Liang Xiaolin Liu Qi Xie Guoling Chen Xingyu Li Yanrui Jia Beibei Yin Xun Qu Yan Li | 2016 | Chinese Journal of Cancer Research2016，28，4： | 8 |
| 2 | Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘The use of periosteum-derived progenitor cells (PCs) combined with bioresorbable materials is an attractive approach for tissue engineering. The aim of this study was to characterize the osteogenic differentiation of PC in 3-dimensional (3D) poly-lactic-co-glycolic acid (PLGA) fleeces cultured in medium containing allogeneic human serum. PCs were isolated and expanded in monolayer culture. Expanded cells of passage 3 were seeded into PLGA constructs and cultured in osteogenic me- dium for a maximum period of 28 d. Morphological, histological and cell viability analyses of three-dimensionally cultured PCs were performed to elucidate osseous synthesis and deposition of a calcified matrix. Furthermore, the mRNA expression of type I collagen, osteocalcin and osteonectin was semi-quantitively evaluated by real-time reverse transcriptase-polymerase chain reac- tion (RT-PCR). The fibrin gel immobilization technique provided homogeneous PCs distribution in 3D PLGA constructs. Live-dead staining indicated a high viability rate of PCs inside the PLGA scaffolds. Secreted nodules of neo-bone tissue formation and the presence of matrix mineralization were confirmed by positive von Kossa staining. The osteogenic differentiation of PCs was further demonstrated by the detection of type I collagen, osteocalcin and osteonectin gene expression. The results of this study support the concept that this tissue engineering method presents a promising method for creation of new bone in vivo. | ZHENG Yi-xiong RINGE Jochen LIANG Zhong LOCH Alexander CHEN Li SITTINGER Michael | 2006 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2006，7，10： | 8 |
| 3 | The Clinical Correlations of Helicobacter pylori Virulence Factors and Chronic Spontaneous Urticaria显示文摘 | Yi-Chun Chiu Wei-Chen Tai Seng-Kee Chuah Ping-I Hsu Deng-Chyang Wu Keng-Liang Wu Chao-Cheng Huang Ji-Chen Ho Johannes Ring Wen-Chieh Chen Chao-Hung Kuo | 2013 | <journal-title>Gastroenterology Research and Practice2013，，： | 2 |
| 4 | Stabilization and processing of aqueous BaTiO3 suspension with polyacrylic acid显示文摘 | CHEN Z C RING T A LEMAITRE L | 1992 | J Am Ceram Soc1992，75，12： | 2 |
| 5 | HDS reactivities of dibenzothiophenic compounds in a LC-finer LGO and H2S/NH3 inhibition effect显示文摘 | CHEN J RING Z | 2004 | Fuel2004，83，3： | 1 |
| 6 | Effect of aromatics on deep hydrodesulfurization of dibenzothiophene and 4, 6-dimethyldibenzothiophene over NiMo/Al2O3 catalyst显示文摘 | SONG T ZHANG Z CHEN J RING Z YANG H ZHENG Y | 2006 | Energy Fuels2006，20，6： | 1 |
| 7 | HDS, HDN, HDA, and hydrocracking of model compounds over Mo-Ni catalysts with various acidities显示文摘 | DING L ZHENG Y ZHANG Z RING Z CHEN J | 2007 | Appl Catal A: Gen2007，319，1： | 1 |
| 8 | Contribution of hydrogen spillover to the hydrogenation of naphthalene over diluted Pt/RHO catalysts显示文摘 | CHEN H YANG H OMOTOSO O DING L BRIKER Y ZHENG Y RING Z | 2009 | Appl Catal A: Gen2009，358，2： | 1 |
| 9 | Hydrodesulfurization of dibenzothiophenic compounds in a light cycle oil显示文摘 | CHEN J TE M YANG H RING Z | 2003 | Petrol Sci Technol2003，21，56： | 1 |
| 10 | Inhibition of nitrogen compounds on the hydrodesulfurization of substituted dibenzothiophenes in light cycle oil显示文摘 | over YANG H CHEN J W FAIRBRIDGE C BRIKER Y ZHU Y J RING Z | 2004 | Fuel Process Technol2004，85，12： | 1 |
| 11 | Terrible Triad Injuries of the Elbow显示文摘 | Neal C. Chen David Ring | 2015 | Journal of Hand Surgery2015，，： | 1 |
| 12 | Shape selective and hydrogen spillover approach in the design of sulfur-tolerant hydrogenation catalysts显示文摘 | YANG H CHEN H CHEN J OMOTOSO O RING Z | 2006 | J Catal2006，243，1： | 1 |
| 13 | Incorporating platinum precursors into a NaA-zeolite synthesis mixture promoting the formation of nanosized zeolite显示文摘 | YANG H CHEN H DU H HAWKINS R CRAIG F RING Z OMOTOSO O MUNOZ V MIKULA R | 2009 | Microprous Mesprous Mater2009，117，1： | 1 |
| 14 | An overview of the analysis of next generation sequencing data显示文摘 | Gogol-D?ring A Chen W | | 0，，： | 1 |
| 15 | Osteogenic potential of human periosteum-derived progenitor cells in PLGA scaffold using allogeneic serum显示文摘 | Yi-xiong Zheng Jochen Ringe Zhong Liang Alexander Loch Li Chen Michael Sittinger | 2006 | Journal of Zhejiang University SCIENCE B2006，，10： | 1 |
| 16 | Shape selective and hydrogen spillover approach in the design of sulfur-tolerant hydrogenation catalysts显示文摘 | YANG H CHEN H L CHEN J W OMOTOSO O RING Z | 2006 | J Catal2006，243，1： | 1 |
| 17 | HDS kinetics study of dibenzothiophenic compounds in LCO 显示文摘 | CHEN J YANG H RING Z | 2004 | Catalysis Today2004，98，1： | 1 |
| 18 | Study of intra-particle diffusion effect on hydrodesulphurization of dibenzothiophenic compounds 显示文摘 | CHEN J W YANG H RING Z | 2005 | Catalysis Today2005，109，14： | 1 |
| 19 | Solvent - free aerobic oxidation of benzyl alcohol over Pd monometallic and Au - Pd bimetallic catalysts supported on SBA -16 mesoporous molecular sieves 显示文摘 | CHEN Yuan - ring LIM Hui - min TANG Qing - hu | 2010 | Applied Catalysis A: General2010，380，： | 1 |
| 20 | HDS, HDN, HAD and hydrocracking of model compounds over Mo-Ni catalysts with various acidities显示文摘 | DING L H ZHENG Y ZHANG Z S RING Z CHEN J W | 2007 | Appl Catal A2007，319，1： | 1 |
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