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| 1 | Isolation and characterization of two degradation products derived from the peptide antibiotic nisin显示文摘 | Chan WC Bycroft BW Lian LY | 1989 | FEBS Lett1989，252，： | 1 |
| 2 | Evaluation of real-time reverse transcriptase PER and real-time loop-mediated amplification assays for severe acute respiratory syndrome cororlavirus detection显示文摘 | Pooh LL Wong BW Chan KH | 2005 | J Clin Microbiol2005，43，7： | 1 |
| 3 | Fibrinolytic response to venous occlusion is decreased in patients after Kawasaki disease显示文摘 | Albisetti M Chan AK McCrindle BW | 2003 | Blood Coagul Fibrinolysis2003，14，2： | 1 |
| 4 | Fribrinolytic response to venous occlusion is decreased in patients after Kawasaki disease 显示文摘 | Albisetti M Chan AK McCrindle BW | 2003 | Blood Coagul Fibrinolysis2003，14，2： | 1 |
| 5 | Depiction of pelvic fractures using 3D volumetric holography: comparision of plain X-ray and CT显示文摘 | Robertson DD Sutherland CJ Chan BW | 1995 | Comput Assist Tomogr1995，19，6： | 1 |
| 6 | Soluble MUC1 secreted by human epithelial cancer cells mediates immune suppression by blocking T- cell activation显示文摘 | Chan AK Lockhart DC Von BW | 1999 | Int J Cancer1999，82，： | 1 |
| 7 | Fibrinolytic response to venous occlusion is decreased in patients after kawasaki disease显示文摘 | Albisetti M Chan AK McCrindle BW et al | 2003 | Bood Coagul Fibrinolysis2003，14，： | 1 |
| 8 | Impaiment of the fi- bfinolytic system is presnt and is a marker for endothelial dysfunc- tion in adolescents after disease 显示文摘 | McCrindle BW Albisetti M Chan AKC | 2003 | J Pediatric Res2003，53，： | 1 |
| 9 | Depiction of pelvic fractures using 3D volumetric holography:comparison of plain X-ray and CT显示文摘 | Robertson DD Sutherland CJ Chan BW | 1995 | J Comput Assist Tomogr1995，19，6： | 1 |
| 10 | Fibrinolytic response to venous occlusion is decreased in patients after Kawasaki disease显示文摘 | Albisetti M Chan AK McCrindle BW et el | 2003 | Blood Coagul Fibrinolysis2003，14，2： | 1 |
| 11 | Torsional strength estimates of femoral diaphyses with endosteal lytic lesions: dual-energy X-ray absorptiometry study 显示文摘 | Robertson DD Beck T J Chan BW et aI | 2007 | Journal of orthopaedic research2007，25，10： | 1 |
| 12 | Controlling Inelastic Light Scattering Quantum Pathways in Graphene显示文摘 | Chan C F CH Park BW Boudouris | 2011 | Nature2011，471，： | 1 |
| 13 | Activation of p38mapk, MKK3, and MKK4 by TNF - alpha in mouse bone marrow - de- rived macrophages 显示文摘 | Winston BW Chan ED Johnson GL | 1997 | J Immunol1997，159，9： | 1 |
| 14 | Use of an electronic barcode system for patient identification during blood transfusion: 3-year experience in a regional hospital 显示文摘 | Chan JC Chu RW Young BW | 2004 | Hong Kong Med J2004，10，3： | 1 |
| 15 | Evaluation of the role of mitogen-activated protein kinases in the expression of inducible nitric oxide synthase by IFN-gamma and TNF-alpha in mouse macrophages 显示文摘 | Winston BW Uh ST | 1999 | J Immunol1999，162，： | 1 |
| 16 | Conversion of cyclosporine to tacrolimus for refractory or persistent myocardial rejection显示文摘 | Chan MC Kwok BW Shiba N | 2002 | Transplant Proc2002，34，： | 1 |
| 17 | Fibrinolytie response to venous occlusion is decreased in patients after Kawasaki disease 显示文摘 | Albisetti M Chan AK McCrindle BW | 2003 | Blood Coagul Fibrinolysis2003，14，2： | 1 |
| 18 | Spiral CT during arterial portography:Technique and applications显示文摘 | Bluemke DA Soyer PA Chan BW | 1995 | RadioGraphics1995，15，： | 1 |
| 19 | Comparison ofpyrosequencing,Sanger sequencing,and melting curveanalysis for detection of low-frequency macrolide-resistantMycoplasma pneumoniae quasispecies in respiratory specimens显示文摘 | Chan KH To KK Chan BW | 2013 | J Clin Microbiol2013，51，8： | 1 |
| 20 | Green tea extract as a cryoprotectant additive to preserve the motility and DNA integrity of human spermatozoa显示文摘Cryopreservation impairs sperm quality and functions,including motility and DNA integrity.Antioxidant additives in sperm freezing media have previously brought improvements in postthawed sperm quality.Green tea extract(GTE)is widely considered as an excellent antioxidant,and its beneficial role has been proven in other human cells.This study aims to evaluate the GTE as a potential additive in cryopreservation media of human spermatozoa.In part one,the semen of 20 normozoospermic men was used to optimize the concentration of GTE that maintains sperm motility and DNA integrity against oxidative stress,induced by hydrogen peroxide(H_(2)O_(2)).Spermatozoa were treated with GTE at different concentrations before incubation with H_(2)O_(2).In part two,the semen of 45 patients was cryopreserved with or without 1.0 ng ml^(-1)GTE.After 2 weeks,the semen was thawed,and the effect on sperm motility and DNA fragmentation was observed.Our data showed that GTE significantly protected sperm motility and DNA integrity against oxidative stress induced by H_(2)O_(2)when added at a final concentration of 1.0 ng ml^(-1).We found that the addition of 1.0 ng ml^(-1)GTE to cryopreservation media significantly increased sperm motility and DNA integrity(both P<0.05).More interestingly,patients with high sperm DNA damage benefited similarly from the GTE supplementation.However,there was no significant change in the reactive oxygen species(ROS)level.In conclusion,supplementing sperm freezing media with GTE has a significant protective effect on human sperm motility and DNA integrity,which may be of clinical interest. | Odai AM Alqawasmeh Mingpeng Zhao Carol PS Chan Maran BW Leung Ki C Chow Nikunj Agarwal Jennifer SM Mak Chi C Wang Chi P Pang Tin C Li Wai K Chu David YL Chan | 2021 | Asian Journal of Andrology2021，23，2： | 0 |
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