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| 1 | The spatial pattern of farming and factors influencing it during the Peiligang culture period in the middle Yellow River valley, China显示文摘The Peiligang culture(ca.7000–5000 cal BC)is the earliest Neolithic culture in the middle Yellow River valley and represents the emergence of cereal farming in the region[1].It has long been considered representative of millet farming[2],which requires a dry field,a certain amount of rainfall,and minimal crop management,but new records reveal that mixed farming of millet and rice(Oryza sp.)took place during this period[3].Therefore,it is possible | Can Wang Houyuan Lu Wanfa Gu Naiqin Wu Jianping Zhang Xinxin Zuo Fengjiang Li Daojing Wang Yajie Dong Songzhi Wang Yanfeng Liu Yingjian Bao Yayi Hu | 2017 | Science Bulletin2017，62，23： | 6 |
| 2 | Hic74,a novel alanine and glycine rich matrix protein related to nacreous layer formation in the mollusc Hyriopsis cumingii显示文摘Organic matrix proteins play an important role in the biomineralization of mollusc shells.We have identified and characterized a novel matrix protein,namely hic74,isolated from the mantle of the freshwater mussel Hyriopsis cumingii.The hic74 gene encodes a 850 amino acid protein that is rich in alanine(ala,30.8%),glycine(gly,25.8%)and serine(ser,10.6%),with ala mainly existing in poly-ala forms within the ala/gly-rich regions.Quantitative PCR expression analysis demonstrated that hic74 was specifically amplified from the mantle and in situ hybridization showed a strong signal in the epithelial cells in the pallial region of the mantle.Lower levels of expression were detected during the early stages of pearl sac formation but increased and remained constant during nacreous layer formation suggesting that hic74 might be involved in this process. | Xiaojun Liu Can Jin Leiming Wu Shaojian Dong Shimei Zeng Jiale Li | 2017 | Aquaculture and Fisheries2017，2，3： | 6 |
| 3 | Microstructure and dry sliding wear behavior of laser clad AlCrNiSiTi multi-principal element alloy coatings显示文摘The approximately equimolar ratio AlCrNiSiTi multi-principal element alloy(MPEA) coatings were fabricated by laser cladding on Ti-6A1-4V(Ti64) alloy.Scanning electron microscopy(SEM),equipped with an energy-dispersive spectroscopy(EDS),and X-ray diffraction(XRD) were used to characterize the microstructure and composition.Investigations show that the coatings consist of(Ti,Cr)_5Si_3 and NiAl phases,formed by in situ reaction.The phase composition is initially explicated according to obtainable binary and ternary phase diagrams,and the formation Gibbs energy of Ti_5Si_3,V_5Si_3 and Cr_5Si_3.Dry sliding reciprocating friction and wear tests of the AlCrNiSiTi coating and Ti64 alloy substrate without coating were evaluated.A surface mapping profiler was used to evaluate the wear volume.The worn surface was characterized by SEM-EDS.The hardness and wear resistance of the AlCrNiSiTi coating are well compared with that of the basal material(Ti64).The main wear mechanism of the AlCrNiSiTi coating is slightly adhesive transfer from GCr15 counterpart,and a mixed layer composed of transferred materials and oxide is formed. | Can Huang Yi-Zhou Tang Yong-Zhong Zhang An-Ping Dong Jian Tu Lin-Jiang Chai Zhi-Ming Zhou | 2017 | Rare Metals2017，36，7： | 5 |
| 4 | Influence of Blade Outlet Angle on Inner Flow Field of Centrifugal Pump Transporting Salt Aqueous Solution显示文摘During transportation of salt aqueous solutions with centrifugal pump, crystallization phenomenon is frequently encountered. For this kind of two-phase flow, it is difficult to be accurately modeled since there are various medium properties and phase change characteristics. In view of experiment, several problems are hampering the implementation of precise measurement. Influences of blade outlet angle and medium temperature on crystallization rate were studied. Sodium sulfate solution was applied to simulate practical fluid in chemical industry. Particle image velocimetry(PIV) was employed to measure velocity distributions in rotating impeller. Crystallization processes in three impellers with different blade outlet angles were investigated. Relations among crystallization and flow parameters such as temperature and velocity were obtained. With the same blade wrap angle, when blade outlet angle is larger, diffusion of single flow passage gets stronger, relative velocity at blade outlet decreases and large scale vortex tends to appear near the blade working surface. For the impact of volume effect of particle phase on fluid viscosity, both liquid and solid phase velocities decrease with continual forming and growing of crystal particles. Velocity of solid phase is greater than that of liquid phase and its direction leans more closely to blade working surface. Solid particles tend to move towards blade working surface, as is more obvious in the impeller with large blade outlet angle. Therefore, collision between solid particles with stern part of blade working surface is more intensive in impeller with large blade outlet angle. Concerning transportation of salt aqueous solution, accurate PIV measurement is conducted in centrifugal impellers with different blade outlet angles. The results are useful and instructive in relevant engineering design and operation. | YANG Minguan KANG Can DONG Xiang LIU Dong | 2009 | Chinese Journal of Mechanical Engineering2009，22，6： | 5 |
| 5 | A new Multilocus Sequence Analysis Scheme for Mycobacterium tuberculosis显示文摘Objective Tuberculosis remains one of the most serious infectious diseases in the world. In this study, a scheme of Mycobacterium tuberculosis (M. tuberculosis) multilocus sequence analysis (MLSA) was established for the phylogenetic and epidemiology analysis. Methods To establish the scheme of M. tuberculosis MLSA, the genome of H37Rv, CCDC5079 and CCDC5180 were compared, and some variable genes were chosen to be the MLSA typing scheme. 44 M. tuberculosis clinical isolates were typed by MLSA, IS6110-RFLP, and soligotyping, to evaluate the MLSA methods. Results After comparison of the genome, seven high discrimination gene loci (recX, rpsL, rmlC, rpmG1, mprA, gcvH, ideR) were chosen to be the MLSA typing scheme finally. 11 variable SNP sites of those seven genes were found among the 44 M. tuberculosis isolate strains and 11 sequence types (STs) were identified. Based on the Hunter-Gaston Index (HGI), MLSA typing was not as good for discrimination at the strain level as IS6110-RFLP, but the HGI was much better than that of spoligotyping. In addition, the MEGA analysis result of MLSA data was similar to spoligotyping/PGG lineage, showing a strong phylogenetic signal in the modern strains of M. tuberculosis. The MLSA data analysis by eBURST revealed that 4 sequence types (ST) came into a main cluster, showing the major clonal complexes in those 44 strains. Conclusion MLSA genotyping not only can be used for molecular typing, but also is an ideal method for the phylogenetic analysis for M. tuberculosis. | LU Bing DONG Hai Yan ZHAO Xiu Qin LIU Zhi Guang LIU Hai Can ZHANG Yuan Yuan JIANG Yi WAN Kang Lin | 2012 | Biomedical and Environmental Sciences2012，25，6： | 4 |
| 6 | Phosphorylation inhibition of protein-tyrosine phosphatase 1B tyrosine-152 induces bone regeneration coupled with angiogenesis for bone tissue engineering显示文摘A close relationship has been reported to exist between cadherin-mediated cell-cell adhesion and integrin-mediated cell mobility,and protein tyrosine phosphatase 1B(PTP1B)may be involved in maintaining this homeostasis.The stable residence of mesenchymal stem cells(MSCs)and endothelial cells(ECs)in their niches is closely related to the regulation of PTP1B.However,the exact role of the departure of MSCs and ECs from their niches during bone regeneration is largely unknown.Here,we show that the phosphorylation state of PTP1B tyrosine-152(Y152)plays a central role in initiating the departure of these cells from their niches and their subsequent recruitment to bone defects.Based on our previous design of a PTP1B Y152 region-mimicking peptide(152RM)that significantly inhibits the phosphorylation of PTP1B Y152,further investigations revealed that 152RM enhanced cell migration partly via integrinαvβ3 and promoted MSCs osteogenic differentiation partly by inhibiting ATF3.Moreover,152RM induced type H vessels formation by activating Notch signaling.Demineralized bone matrix(DBM)scaffolds were fabricated with mesoporous silica nanoparticles(MSNs),and 152RM was then loaded onto them by electrostatic adsorption.The DBM-MSN/152RM scaffolds were demonstrated to induce bone formation and type H vessels expansion in vivo.In conclusion,our data reveal that 152RM contributes to bone formation by coupling osteogenesis with angiogenesis,which may offer a potential therapeutic strategy for bone defects. | Yong Tang Keyu Luo Yin Chen Yueqi Chen Rui Zhou Can Chen Jiulin Tan Moyuan Deng Qijie Dai Xueke Yu Jian Liu Chengmin Zhang Wenjie Wu Jianzhong Xu Shiwu Dong Fei Luo | 2021 | Bioactive Materials2021，6，7： | 3 |
| 7 | Particle size distributions, PM2.5 concentrations and water- soluble inorganic ions in different public indoor environments: a case study in Jinan, China显示文摘In this study, we collected particles with aerodynamic diameter≤2.5μm (PM2.5) from three different public indoor places (a supermarket, a commercial office, and a university dining hall) in Jinan, a medium-sized city located in northern China. Water- soluble inorganic ions of PM2.5 and particle size distributions were also measured. Both indoor and outdoor PM2.5 levels (102.3-143.8 μg·m- 3 and 160.2-301.3 μg·m-3, respectively) were substantially higher than the value recommended by the World Health Organization (25 I.μg·m-3), and outdoor sources were found to be the major contributors to indoor pollutants. Diurnal particle number size distributions were different, while the maximum volume concentrations all appeared to be approximately 300nm in the three indoor locations. Concentrations of indoor and outdoor PM2.5 were shown to exhibit the same variation trends for the supermarket and dining hall. For the office, PM2.5 concentrations during nighttime were observed to decrease sharply. Among others, SO4^2-, NH4+ and NO3- were found to be the dominant water-soluble ions of both indoor and outdoor particles. Concentrations of NO3- in the supermarket and office during the daytime were observed to decrease sharply, which might be attributed to the fact that the indoor temperature was much higher than the outdoor temperature. In addition, domestic activities such as cleaning, water usage, cooking, and smoking also played roles in degraded indoor air quality. However, the results obtained here might be negatively impacted by the small number of samples and short sampling durations. | Can DONG Lingxiao YANG Chao YAN Qi YUAN Yangchun YU Wenxing WANG | 2013 | Frontiers of Environmental Science & Engineering2013，7，1： | 3 |
| 8 | Hundred-Joule-level,nanosecond-pulse Nd:glass laser system with high spatiotemporal beam quality显示文摘A 100-J-level Nd:glass laser system in nanosecond-scale pulse width has been constructed to perform as a standard source of high-fluence-laser science experiments. The laser system, operating with typical pulse durations of 3–5 ns and beam diameter 60 mm, employs a sequence of successive rod amplifiers to achieve 100-J-level energy at 1053 nm at3 ns. The frequency conversion can provide energy of 50-J level at 351 nm. In addition to the high stability of the energy output, the most valuable of the laser system is the high spatiotemporal beam quality of the output, which contains the uniform square pulse waveform, the uniform flat-top spatial fluence distribution and the uniform flat-top wavefront. | Sensen Li Yulei Wang Zhiwei Lu Lei Ding Yi Chen Pengyuan Du Dexin Ba Zhenxing Zheng Xin Wang Hang Yuan Chengyu Zhu Weiming He Dianyang Lin Yongkang Dong Dengwang Zhou Zhenxu Bai Zhaohong Liu Can Cui | 2016 | High Power Laser Science and Engineering2016，4，1： | 3 |
| 9 | Species,engineering and characterizations of defects in TiO2-basedphotocatalyst显示文摘Light absorption, charge separation and surface reaction are considered as the main processes of photocatalysis on one semiconductor, and all of them are demonstrated to be related to the defect states of photocatalysts. This paper will choose TiO_2 as model photocatalyst to introduce some basic concepts and strategies related to defects and methods developed to characterize defects in the past decades.Meanwhile, such strategies as hydrogenation and metal/nonmetal doping into TiO_2 will be introduced to extend utilization of solar spectrum and/or to provide active sites. On the contrary, the unfavorable effect of defects such as acting as recombination centers of photogenerated carriers will also be introduced.Some typical methods to characterize the properties of defects are summarized, which contain electron paramagnetic resonance(EPR), photoluminescence technique(PL), positron annihilation spectroscopy(PAS), and so on. We do hope that this review will make a revealing effect on understanding to the functions of defects as well as construction of efficient photocatalytic systems in the future. | Beibei Dong Taifeng Liu Can Li Fuxiang Zhang | 2018 | Chinese Chemical Letters2018，29，5： | 3 |
| 10 | Cell-based drug delivery systems for biomedical applications显示文摘 | Teng Li He Dong Can Zhang Ran Mo | 2018 | Nano Research2018，11，10： | 3 |
| 11 | Short- and Long-term Therapeutic Efficacies of Intravenous Transplantation of Bone Marrow Stem Cells on Cardiac Function in Rats with Acute Myocardial Infarction:A Meta-analysis of Randomized Controlled Trials显示文摘Objective To investigate the short- and long-term therapeutic efficacies of intravenous transplantation of bone marrow stem cells(MSCs) in rats with experimental myocardial infarction by metaanalysis.Methods Randomized controlled trials were systematically searched from Pub Med,Science Citation Index(SCI),Chinese journal full-text database(CJFD) up to December 2014.While the experimental groups(MSCs groups) were injected MSCs intravenously,the control groups were injected Delubecco's minimum essential medium(DMEM) or phosphate buffered saline(PBS).Subgroup analysis for each outcome measure was performed for the observing time point after the transplantation of MSCs.Weighted mean differences(WMD) and 95% confidence intervals(CI) were calculated for outcome parameters including ejection fraction(EF) and fractional shortening(FS),which were measured by echocardiogram after intravenous injection and analyzed by Rev Man 5.2 and STATA 12.0.Results Data from 9 studies(190 rats) were included in the meta-analysis.As compared to the control groups,the cardiac function of the experimental groups were not improved at day 7(EF:WMD=0.08,95%CI-1.32 to 1.16,P>0.01; FS:WMD=-0.12,95%CI-0.90 to 0.65,P>0.01) until at day 14 after MSCs' transplantation(EF:WMD=10.79,95%CI 9.16 to 12.42,P<0.01; FS:WMD=11.34,95%CI 10.44 to 12.23,P<0.01),and it lasted 4 weeks or more after transplantation of MSCs(EF:WMD=13.94,95%CI 12.24 to 15.64,P<0.01; FS:WMD=9.64,95%CI 7.98 to 11.31,P<0.01).Conclusion The therapeutic efficacies of MSCs in rats with myocardid infarction become increasing apparent as time advances since 2 weeks after injection. | Can Jiang Dong Zheng Yun-lu Feng Jun Guo Hai-rui Li Ai-dong Zhang | 2016 | Chinese Medical Sciences Journal2016，31，3： | 2 |
| 12 | Overview to the Hard X-ray Modulation Telescope (Insight-HXMT) Satellite显示文摘As China’s first X-ray astronomical satellite, the Hard X-ray Modulation Telescope (HXMT), which was dubbed as Insight-HXMT after the launch on June 15, 2017, is a wide-band(1-250 ke V) slat-collimator-based X-ray astronomy satellite with the capability of all-sky monitoring in 0.2-3 Me V. It was designed to perform pointing, scanning and gamma-ray burst(GRB)observations and, based on the Direct Demodulation Method (DDM), the image of the scanned sky region can be reconstructed.Here we give an overview of the mission and its progresses, including payload, core sciences, ground calibration/facility, ground segment, data archive, software, in-orbit performance, calibration, background model, observations and some preliminary results. | Shuang-Nan Zhang TiPei Li FangJun Lu LiMing Song YuPeng X u CongZhan Liu Yong Chen XueLei Cao QingCui Bu Zhi Chang Gang Chen Li Chen TianXiang Chen YiBao Chen YuPeng Chen Wei Cui WeiWei Cui JingKang Deng YongWei Dong Yuan Yuan Du MinXue Fu GuanHua Gao He Gao Min Gao MingYu Ge YuDong Gu Ju Guan Can Gungor ChengCheng Guo DaWei Han Wei Hu Yue Huang Jia Huo ShuMei Jia LuHua Jiang WeiChun Jiang Jing Jin YongJie Jin Bing Li ChengKui Li Gang Li MaoShun Li Wei Li Xian Li XiaoBo Li XuFang Li YanGuo Li ZiJian Li ZhengWei Li XiaoHua Liang JinYuan Liao GuoQing Liu HongWei Liu ShaoZhen Liu XiaoJing Liu Yuan Liu YiNong Liu Bo Lu XueFeng Lu Tao Luo Xiang Ma Bin Meng Yi Nang JianYin Nie Ge Ou JinLu Qu Na Sai RenCheng Shang GuoHong Shen Liang Sun Ying Tan Lian Tao YouLi Tuo Chen Wang ChunQin Wang GuoFeng Wang HuanYu Wang Juan Wang WenShuai Wang YuSa Wang XiangYang Wen BaiYang Wu BoBing Wu Mei Wu GuangCheng Xiao ShaoLin Xiong LinLi Yan JiaWei Yang Sheng Yang YanJi Yang QiBin Yi Bin Yuan AiMei Zhang ChunLei Zhang ChengMo Zhang Fan Zhang HongMei Zhang Juan Zhang Qiang Zhang ShenYi Zhangs Shu Zhang Tong Zhang WanChang Zhang Wei Zhang WenZhao Zhang Yi Zhang YiFei Zhang YongJie Zhang Yue Zhang Zhao Zhang Zhi Zhang ZiLiang Zhang HaiSheng Zhao XiaoFan Zhao ShiJie Zheng JianFeng Zhou YuXuan Zhu Yue Zhu RenLin Zhuang | 2020 | Science China(Physics,Mechanics & Astronomy)2020，63，4： | 2 |
| 13 | EUS-guided celiac ganglion irradiation with iodine-125 seeds for pain control in pancreatic carcinoma: a prospective pilot study显示文摘 | Kai-Xuan Wang Zhen-Dong Jin Yi-Qi Du Xian-Bao Zhan Duo-Wu Zou Yan Liu Dong Wang Jie Chen Can Xu Zhao-Shen Li | 2012 | Gastrointestinal Endoscopy2012，，5： | 2 |
| 14 | The Current Status and Challenges Regarding Tuberculosis Infection Control in Health Care Facilities in China显示文摘Health care facilities are a high-risk environment for generating and spreading respiratory infectious diseases such as tuberculosis(TB).The TB prevention and treatment staff in health care facilities are responsible for the identification,diagnosis,treatment,supervision,and management of | ZHAO Fei CHENG Jun CHENG Shi Ming ZHANG Hui ZHAO Yan Lin ZHANG Can You HU Dong Mei FAN Hai Ying HUANG Fei QU Yan HE Guang Xue WANG Li Xia | 2015 | Biomedical and Environmental Sciences2015，28，11： | 2 |
| 15 | Diagnostic and economic value of carcinoembryonic antigen,carbohydrate antigen 19-9,and carbohydrate antigen 72-4 in gastrointestinal cancers显示文摘BACKGROUND The diagnostic and economic value of carcinoembryonic antigen(CEA),carbohydrate antigen 19-9(CA19-9)and CA72-4 for gastrointestinal malignant tumors lacked evaluation in a larger scale.AIM To reassess the diagnostic and economic value of the three tumor biomarkers.METHODS A retrospective analysis of all 32857 subjects who underwent CEA,CA19-9,CA72-4,gastroscopy and colonoscopy from October 2006 to May 2018 was conducted.Then,we assessed the discrimination and clinical usefulness.Total cost,cost per capita and cost-effectiveness ratios were used to evaluate the economic value of two schemes(gastrointestinal endoscopy for all people without blood tests vs both gastroscopy and colonoscopy when blood tests were positive).RESULTS The analysis of 32857 subjects showed that CEA was a qualified biomarker for colorectal cancer(CRC),while the diagnostic efficiencies of CA72-4 were catastrophic for all gastrointestinal cancers(GICs).Regarding early diagnosis,only CEA could be used for early CRC.The combination of biomarkers didn’t greatly increase the area under the curve.The economic indicators of CEA were superior to those of CA19-9,CA72-4 and any combination.At the threshold of 1.8μg/L to 10.4μg/L,all four indicators of CEA were lower than those in the scheme that conducted gastrointestinal endoscopy only.Subgroup analysis implied that the health checkup of CEA for people above 65 years old was economically valuable.CONCLUSION CEA had qualified diagnostic value for CRC and superior economic value for GICs,especially for elderly health checkup subjects.CA72-4 was not suitable as a diagnostic biomarker. | Hai-Ning Liu Can Yao Xiao-Fan Wang Ning-Ping Zhang Yan-Jie Chen Dong Pan Guo-Ping Zhao Xi-Zhong Shen Hao Wu Tao-Tao Liu | 2023 | World Journal of Gastroenterology2023，29，4： | 2 |
| 16 | Occupational Health Risk Assessment of Benzene,Toluene,and Xylene in Shanghai显示文摘Objective This study was designed to conduct a retrospective and systematic occupational health risk assessment(OHRA)of enterprises that used benzene,toluene,and xylene(BTX)in Shanghai,China.Methods All data for the study were obtained from 1,705 occupational health examination and evaluation reports from 2013 to 2017,and a semiquantitative model following Chinese OHRA guidelines(GBZ/T 298-2017)was applied for the assessment.Results The selected enterprises using BTX were mainly involved in manufacturing of products.Using the exposure level method,health risk levels associated with exposure to BTX were classified as medium,negligible,or low.However,the risk levels associated with benzene and toluene were significantly different according to job types,with gluers and inkers exhibiting greater health risks.For the same job type,the health risk levels assessed using the comprehensive index method were higher than those using the exposure level method.Conclusion Our OHRA reveals that workers who are exposed to BTX still face excessive health risk.Additionally,the risk level varied depending on job categories and exposure to specific chemicals.Therefore,additional control measures recommended by OHRA guidelines are essential to reduce worker exposure levels. | WANG Tong Shuai SONG Bo SUN Qing Hua LIN Yong Xin SUN Yuan SUN Pin JIA Xiao Dong William W.Au MEI Can Hua XIA Zhao Lin | 2021 | Biomedical and Environmental Sciences2021，34，4： | 2 |
| 17 | A synchronization approach to trajectory tracking of multiple mobile robots while maintaining time-varying formations 显示文摘 | SUN Dong WANG Can SHANG Wen FENG Guang | 2009 | IEEE Transactions on Robotics2009，25，10： | 1 |
| 18 | Detecion and location of Heli--cobacter pylori in human gastric carcinomas显示文摘 | Tang Y L Can R L Dong B H | 2005 | World J Gastroenterol2005，11，9： | 1 |
| 19 | First Report in China on the Identification and Drug Sensitivity of Mycobacterium elephantis Isolated from the Milk of a Cow with Mastitis显示文摘Objective In this study, milk from a cow with mastitis was analyzed to determine the presence of mycobacterial infection. Milk quality and security problems pertaining to the safe consumption of dairy products were also discussed in this study. Methods Milk was preprocessed with 4% Na OH. Then, mycobacteria were isolated from the milk sample on L-J medium. The isolate was identified using multiple loci Polymerase Chain Reaction(PCR) and multi-locus sequence analysis with 16 S r RNA, sod A, hsp65, and ITS genes. The drug sensitivity of the isolate to 27 antibiotics was tested through alamar blue assay. Results Smooth, moist, pale yellow colonies appeared on the L-J medium within a week after inoculation. Based on the results of multiple loci PCR analysis, the isolate was preliminarily identified as non-tuberculous mycobacteria. The 16 S r RNA, Sod A, hsp65, and ITS gene sequences of the isolate exhibited 99%, 99%, 99%, and 100% similarities, respectively, with those of the published reference strains of Mycobacterium elephantis(M. elephantis). The drug sensitivity results showed that the strain is resistant to isoniazid, p-aminosalicylic acid, and trimesulf but is sensitive to ofloxacin, rifampicin, amikacin, capreomycin, moxifloxacin, kanamycin, levofloxacin, cycloserine, ethambutol, streptomycin, tobramycin, rifabutin, ciprofloxacin, linezolid, cefoxitin, clarithromycin, and minocycline. Conclusion To the best of our knowledge, this study is initially to report the isolation of M. elephantis from the milk of a cow with mastitis in China. | JI Ling Yun XU Dong Lei YIN Shu Peng LIU Hai Can LI Gui Lian JIANG Yi WEI Jian Hao ZENG Hao LOU Yong Liang LYU Jian Xin WAN Kang Lin | 2017 | Biomedical and Environmental Sciences2017，30，7： | 1 |
| 20 | Molecular cloning and functional characterization of a soybean GmG MP1 gene reveals its involvement in ascorbic acid biosynthesis and multiple abiotic stress tolerance in transgenic plants显示文摘L-Ascorbic acid(As A) plays an important role in plants and animals. In plants, GDP-D-mannose pyrophosphorylase(GMP) is essential in the As A biosynthetic pathway. However, little is known about the genes encoding GMP in soybean and here we report genetic and functional analysis of the Gm GMP1(Glycine max GDP-D-mannose pyrophosphorylase 1) gene in this species. Gm GMP1 encoded a GDP-mannose pyrophosphorylase and exhibited higher transcript levels in the leaf than in the root, stem, flower, and seed. Transcript of this gene was ubiquitous in the vegetative and reproductive organs, and was induced by abiotic stress and light. Increasing expression of Gm GMP1 in Arabidopsis and soybean through an overexpressing approach caused pronounced enhancement of As A content, and was implicated in lowering the superoxide anion radical content and lipid peroxidation levels in Arabidopsis, and conferring tolerance to osmotic and high salt stresses during seed germination. The present study represents the first systematic determination of soybean genes encoding GDP-mannose pyrophosphorylase and provides useful evidence for the functional involvement of Gm GMP1 in control of As A content and conferring tolerance to osmotic and salt stress. | XUE Chen-chen XU Jin-yan WANG Can GUO Na HOU Jin-feng XUE Dong ZHAO Jin-ming XING Han | 2018 | Journal of Integrative Agriculture2018，17，3： | 1 |
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