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| 1 | Virulence factors of Enterococcus strains isolated from patients with inflammatory bowel disease显示文摘AIM: To determine the features of Enterococcus that contribute to the development and maintenance of the inflammatory process in patients with inflammatory bowel disease (IBD). METHODS: Multiplex polymerase chain reaction (PCR) was applied to assess the presence of genes that encode virulence factors [surface aggregating protein (asa1), gelatinase (gelE), cytolysin (cylA), extracellular surface protein (esp) and hyaluronidase (hyl)] in the genomic DNA of 28 strains of Enterococcus isolated from the intestinal tissues of children with IBD (n =16) and of children without IBD (controls; n = 12). Additionally, strains with confirmed presence of the gelE gene were tested by PCR for the presence of quorum sensing genes (fsrA, fsrB, fsrC) that control the gelatinase production. Gelatinase activity was tested on agar plates containing 1.6% gelatin. We also analysed the ability of Enterococcus strains to release and decompose hydrogen peroxide (using Analytical Merckoquant peroxide test strips) and tested their ability to adhere to Caco-2 human gut epithelium cells and form biofilms in vitro. RESULTS: A comparison of the genomes of Enterococcus strains isolated from the inflamed mucosa of patients with IBD with those of the control group showed statistically significant differences in the frequency of theasa1 gene and thegelE gene. Furthermore, the cumulative occurrence of different virulence genes in the genome of a single strain ofEnterococcus isolated from the IBD patient group is greater than in a strain from the control group, although no significant difference was found. Statistically significant differences in the decomposition of hydrogen peroxide and adherence to the Caco-2 epithelial cell line between the strains from the patient group and control group were demonstrated. The results also showed that profuse biofilm production was more frequent amongEnterococcus strains isolated from children with IBD than in control strains. CONCLUSION: Enterococcus strains that adhere strongly to the intestinal epithelium, form biofilms and possess antioxidant defence mechanisms seem to have the greatest influence on the inflammatory process. | Edyta Golińska Anna Tomusiak Tomasz Gosiewski Gra yna Wiecek Agnieszka Machul Diana Mikoajczyk Magorzata Bulanda Piotr B Heczko Magdalena Strus | 2013 | World Journal of Gastroenterology2013，19，23： | 8 |
| 2 | Paternal involvement with children: The influence of gender ideologies 显示文摘 | Bulanda RE | 2004 | J Marriage Faro2004，66，1： | 1 |
| 3 | Resistance and capacity in thethallus of Marchanitia polymorpha显示文摘 | Paszewski A Bulanda W Dziubinska H | 1982 | Physiologia Plantarum1982，54，： | 1 |
| 4 | Immunohistochemical investigation of selected endothelial markers in pulmonary epithelioid haemangioendothelioma显示文摘 | Biabas M Papla B Bulanda A | | 0，，： | 1 |
| 5 | Gender, marital power, and marital quality in lat- er life显示文摘 | Bulanda JR | 2011 | J Women Aging2011，23，1： | 1 |
| 6 | Perceived parent - child relations and adolescent self-esteem 显示文摘 | BULANDA R E MAJUMDAR D | 2009 | Journal of Child and Family Studies2009，18，2： | 1 |
| 7 | Predicting motor outcome at pre- school age for infants tested at 7,30,60,and 90 days after term age using the Test of Infant Motor Performance显示文摘 | Kolobe TH Bulanda M Susman L | 2004 | Phys Ther2004，84，12： | 1 |
| 8 | Bayesian wavelet estimation from seismic and well data显示文摘 | BulandA Omre H | | 0，，： | 1 |
| 9 | Muhilocus se- quence types of invasive and colonizing neonatal group B strep- tococci in Poland 显示文摘 | Brzychczy-Wloch M Gosiewski T Bulanda M | 2014 | Med Princ Praet2014，23，4： | 1 |
| 10 | Predicting motor outcome atpreschool age for infants tested at 7,30,60,and 90 days afterterm age using the Test of Infant Motor Performance 显示文摘 | Kolobe TH Bulanda M Lkof S | 2004 | 84(12) :1144-11562004，84，12： | 1 |
| 11 | Perceived parent-child relations and adolescent self-esteem 显示文摘 | BULANDA RE MAJUMDAR D | 2009 | J Child Fam Stud2009，18，2： | 1 |
| 12 | BRE银行大楼,比得哥什,波兰显示文摘 | Bulanda i Mucha Architekci | 2005 | 世界建筑2005，，5： | 0 |
| 13 | 旧造纸厂改建,康斯坦钦-耶焦尔纳,波兰显示文摘 | Bulanda i Mucha Architekci | 2005 | 世界建筑2005，，5： | 0 |
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