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| 1 | Brazilian biosafety law and the new breeding technologies显示文摘Globally,the area of land cultivated with genetically modified(GM)crops has increased a thousand-fold over the last two decades.Although this technology has become important for food production,the regulatory frameworks that underpin these outcomes are based on a list of requirements for a risk assessment that differ from country to country.In recent years,policymakers have had the opportunity to learn from the controversies over transgenics to create effective regulatory milestones for emerging technologies,allowing them to reach their potential for a more sustainable agriculture,ensuring food security.In Brazil,Law No.11.105 of 24 March 2005 established a framework with four main organizations responsible for risk assessment and management.However,most of new breeding technologies did not exist at that time and were not considered in this law.In2016,Normative Resolution No.16 of the National Biosafety Technical Commission(CTNBio)was established to address this gap based on the evaluation of the products obtained through these techniques(termed Innovative Precision Improvement Techniques in the resolution),in a case-by-case consultation system.Briefly,if the product is designated to be a GM,the developer will have to go through the biosafety requirements and will be approved only after CTNBio risk assessment.If the product is designated not to be GM(for the purposes of the legislation),then it can be registered using the existing procedures.Currently,152 GM products are commercially approved in Brazil.In 2018,CTNBio assessed the first consultation on commercial release of plants generated using the new breeding technologies and has subsequently approved six products.It is expected that many institutions would be able to participate in Brazilian and world markets,developing and introducing new biotechnological solutions and products through a more sustainable approach and without facing public disapproval,a common issue for GM crops. | Alexandre Lima NEPOMUCENO Renata FUGANTI-PAGLIARINI Maria Sueli Soares FELIPE Hugo Bruno Correa MOLINARI Edivaldo Domingues VELINI Eduardo Romano de Campos PINTO Maria Lucia Zaidan DAGLI Galdino ANDRADE FILHO Patricia Machado Bueno FERNANDES | 2020 | Frontiers of Agricultural Science and Engineering2020，7，2： | 5 |
| 2 | 适时提高出雏器中二氧化碳的水平可生产出高质量的健康雏鸡显示文摘人们一直认为孵化后期较高的二氧化碳浓度会影响胚胎的发育,其实不然。研究发现,适时提高出雏器中二氧化碳的水平有助于入孵种蛋的胚胎从绒毛尿囊膜呼吸转为肺呼吸,就像在自然孵化状态下一样。最终的孵化性能也证实,出壳期周围环境中二氧化碳的水平为0.80%时种蛋的孵化率提高了0.50%,而且雏鸡的质量更高。 | 孙丹彤(译) 王晶晶(校) Bruno Machado Eduardo Romanini 何闪(制图表) | 2020 | 国外畜牧学(猪与禽)2020，40，8： | 2 |
| 3 | Parathyroid ultrasonography and bone metabolic profile of patients on dialysis with hyperparathyroidism显示文摘AIM To evaluate the parathyroid ultrasonography and define parameters that can predict poor response to treatment in patients with secondary hyperparathyroidism due to renal failure.METHODS This cohort study evaluated 85 patients with chronic kidney disease stage V with parathyroid hormone levels above 800 pg/mL. All patients underwent ultrasonography of the parathyroids and the following parameters were analyzed: Demographic characteristics(etiology of chronic kidney disease, gender, age, dialysis vintage, vascular access, use of vitamin D), laboratory(calcium, phosphorus, parathyroid hormone, alkaline phosphatase, bone alkaline phosphatase), and the occurrence of bone changes, cardiovascular events and death. The χ~2 test were used to compare proportions or the Fisher exact test for small sample frequencies. Student t-test was used to detect differences between the two groups regarding continuous variables.RESULTS Fifty-three patients(66.4%) had parathyroid nodules with higher levels of parathyroid hormone, calcium and phosphorus. Sixteen patients underwent parathyroidectomy and had higher levels of phosphorus and calcium × phosphorus product(P = 0.03 and P = 0.006, respectively). They also had lower mortality(32% vs 68%, P = 0.01) and lower incidence of cardiovascular or cerebrovascular events(27% vs 73%, P = 0.02). Calcium × phosphorus product above 55 mg^2/dL^2 [RR 1.48(1.06, 2.08), P = 0.03], presence of vascular calcification [1.33(1.01, 1.76), P = 0.015] and previous occurrence of vascular events [RR 2.25(1.27, 3.98), P < 0.001] were risk factors for mortality in this population. There was no association between the occurrence of nodules and mortality.CONCLUSION The identification of nodules at ultrasonography strengthens the indication for parathyroidectomy in patients with secondary hyperparathyroidism due to renal failure. | Cláudia Ribeiro Maria Goretti Moreira Guimaraes Penido Milena Maria Moreira Guimaraes Marcelo de Sousa Tavares Bruno das Neves Souza Anderson Ferreira Leite Leonardo Martins Caldeira de Deus Lucas Joséde Campos Machado | 2016 | World Journal of Nephrology2016，5，5： | 2 |
| 4 | Synergistic effect between few layer graphene and carbon nanotube supports for palladium catalyzing electrochemical oxidation of alcohols显示文摘Few layer graphene (FLG), multi-walled carbon nanotubes (CNTs) and a nanotube-graphene composite (CNT-FLG) were used as supports for palladium nanoparticles. The catalysts, which were characterized by transmission electron microscopy, Raman spectroscopy and X-ray diffraction, were used as anodes in the electrooxidation of ethanol, ethylene glycol and glycerol in half cells and in passive direct ethanol fuel cells. Upon Pd deposition, a stronger interaction was found to occur between the metal and the nanotube-graphene composite and the particle size was significantly smaller in this material (6.3 nm), comparing with nanotubes and graphene alone (8 and 8.4 nm, respectively). Cyclic voltammetry experiments conducted with Pd/CNT, Pd/FLG and Pd/CNT-FLG in 10 wt% ethanol and 2 M KOH solution, showed high specific currents of 1.48, 2.29 and 2.51 mA μg-1 Pd , respectively. Moreover, the results obtained for ethylene glycol and glycerol oxidation highlighted the excellent electrocatalytic activity of Pd/CNT-FLG in terms of peak current density (up to 3.70 mA μg-1 Pd for ethylene glycol and 1.84 mA μg-1 Pd for glycerol, respectively). Accordingly, Pd/CNT-FLG can be considered as the best performing one among the electrocatalysts ever reported for ethylene glycol oxidation, especially considering the low metal loading used in this work. Direct ethanol fuel cells at room temperature were studied by obtaining power density curves and undertaking galvanostatic experiments. The power density outputs using Pd/CNT, Pd/FLG and Pd/CNT-FLG were 12.1, 16.3 and 18.4 mW cm2 , respectively. A remarkable activity for ethanol electrooxidation was shown by Pd/CNT-FLG anode catalyst. In a constant current experiment, the direct ethanol fuel cell containing Pd/CNT-FLG could continuously deliver 20 mA cm2 for 9.5 h during the conversion of ethanol into acetate of 30%, and the energy released from the cell was about 574 J. | Bruno F. Machado Andrea Marchionni Revathi R. Bacsa Marco Bellini Julien Beausoleil Werner Oberhauser Francesco Vizza Philippe Serp | 2013 | Journal of Energy Chemistry2013，22，2： | 1 |
| 5 | A multi-agent model system for land-use change simulation显示文摘 | Célia G. Ralha Carolina G. Abreu Cássio G.C. Coelho Alexandre Zaghetto Bruno Macchiavello Ricardo B. Machado | 2013 | Environmental Modelling and Software2013，，： | 1 |
| 6 | Transesterification of castor oil in a solvent-free medium using the lipase from Burkholderia cepacia LTEBll immobilized on a hydrophobic support显示文摘 | Alessandra Machado Baron Nathalie Barouh Bruno Barea Pierre Villeneuve David Alexander Mitchell Nadia Krieger | 2014 | Fuel Part A2014，117，： | 1 |
| 7 | Platinum supported on TiO 2 as a new selective catalyst on heterogeneous hydrogenation ofα,β-unsaturated oxosteroids显示文摘 | Rui M D Nunes Bruno F Machado Mariette M Pereira | | 0，，： | 1 |
| 8 | Acute Myocardial Infarction due to Coronary Artery Embolism in a Patient with Mechanical Aortic Valve Prosthesis显示文摘 | Marcelo A. Nakazone Bruno G. Tavares Maurício N. Machado Lilia N. Maia Grigorios Korosoglou | 2010 | Case Reports in Medicine2010，，： | 1 |
| 9 | Carbon xerogel supported noble metal catalysts for fine chemical applications显示文摘 | Bruno F Machado Helder T Gomes Philippe Serp | | 0，，3： | 1 |
| 10 | Catalytic properties of carbon materials for wet oxidationof aniline显示文摘 | Gomes Helder T Machado Bruno F Ribeiro Andreia etal | 2008 | J Hazard Mater2008，159，23： | 1 |
| 11 | Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain显示文摘Objective:To identify whether Canova medication changes TNF-α and IL-10 serum levels in mice infected with Trypanosoma cruzi Y strain.Methods:Animals were divided into five groups:non-treated infected animals(I); benznidazole-treated infected animals(Bz; 100 mg/kg body weight,single daily dose by gavage); Canova medication(CM) treated infected animals(CM;0.2 mL/animal,single daily dose by gavage); benznidazole- and Canova medication–treated infected animals with the above-mentioned dose(Bz+CM);and non-infected animals(C).TNF-α and IL-10 levels were determined in serum aliquots after 4,7,10,13,and 29 days of infection.An ELISA technique was employed with R&D System Inc.antibody pairs.Results:A high increase in TNF-α and IL-10 levels occurred in the infected and CM-treated groups within the treatment employed on the 10 th day after infection,coupled with a IL-10 decrease on the 13 th day after infection when compared with the other experimental groups.Conclusions:CM may change the balance between plasma cytokine levels(TNF-α and IL-10) in mice infected with Y strain T.cruzi,with important consequences leading towards a more severe infection. | áurea Regina Telles Pupulin Lívia Bracht Márcia Machado de Oliveira Dalalio Francielli Maria de Souza Silva-Comar Bruno Ambrósio da Rocha Franciele Queiroz Ames Roberto Kenji Nakamura Cuman Ciomar Aparecida Bersani-Amado | 2016 | Asian Pacific Journal of Tropical Medicine2016，9，9： | 0 |
| 12 | Surface Finish Evaluation of AISI P100 Steel after Grinding with MQL Technique with Different Flow Rates显示文摘Grinding operation is a finishing process often employed when high precision and narrow geometric tolerances are required.These requirements can be achieved only if cutting conditions are properly selected,especially the cooling-lubrication technique.In general,grinding is performed in presence of cutting fluid,however,due to the environmental impacts and costs of the conventional coolant delivery technique(flow rates from 4 L/min to 300 L/min),alternative cooling-lubrication techniques have been developed on restriction of the coolants use.Among the several techniques,MQL(minimum quantity of lubricant)technique has received special attention from machining users because of its advantages in terms of surface quality of workpiece and drastic reduction in use of coolant.In this context,this paper evaluated the performance of the MQL technique as compared to the flood coolant in peripheral surface grinding of AISI P100(VP100)steel with conventional aluminum oxide grinding wheel in relation to the surface roughness(Ra and Rz).Input parameters tested were equivalent chip thickness(0.09μm,0.18μm and 0.27μm)and flow rate of the cutting fluid(60 mL/h,150 mL/h and 240 mL/h)of the MQL system.Results showed that the grinding with MQL technique provided lower surface roughness values compared to conventional flood cooling,especially when machining under the intermediary cutting conditions.Also,with exception of heq of 0.09 m,the MQL technique resulted in lower values of Rz parameter as compared to the conventional coolant technique,regardless of the flow rate tested. | Bruno Souza Abrão Mayara Fernanda Pereira Cleudes Guimarães Lurian Souza Vieira da Silva Rodrigo deSouza Ruzzi Rosemar Batista da Silva Eduardo Carlos Bianchi Alisson Rocha Machado | 2020 | Journal of Mechanics Engineering and Automation2020，10，2： | 0 |
| 13 | Semen lactoferrin promotes CCL20 production by epithelial cells: Involvement in HIV transmission显示文摘AIM: To study the effect of seminal plasma on Chemokine(C-C motif) ligand 20(CCL20) production by epithelial cells and its relationship with lactoferrin.METHODS: HEC-1A cells, a cell line derived from a monostratified endocervical epithelium, were incubated with samples of seminal plasma(diluted 1:10 in culture medium) recovered from human immunodeficiency virus(HIV) seronegative(HIV-) or HIV seropositive(HIV+) subjects. Recombinant human interleukin 1 beta(IL-1β) was used as positive control, and culture medium only as negative control. The measurement of CCL20 production in the supernatants of HEC-1A cells and of lactoferrin in seminal plasma was determined by enzyme-linked immunosorbent assay techniques. A fractionation of seminal plasma proteins was performed by ion exchange chromatography on a pool of seminal plasma specimens from HIV- subjects. Each fraction was tested for its ability to stimulate the production of CCL20 by HEC-1A cells and for its lactoferrin concentration. The HIV viral load in seminal plasma samples from HIV+ patients was measured using the HIV-Monitor kit(Roche Diagnostic Systems, Branchburg, NJ, United States).RESULTS: The positive control IL-1β was responsible for an increase of 11.36 ± 3.36 times in the production of CCL20. Stimulation of HEC-1A cells was performed in 34 seminal plasma samples(22 from HIV+ subjects and 12 from HIV- subjects). The mean production of CCL20 by HEC-1A in presence of seminal plasma from HIV- and HIV+ subjects was respectively 5.38 ± 0.91 and 7.57 ± 3.26 times higher than that obtained with the untreated cells(P < 0.05 between the two groups). Using the same 34 specimens of seminal plasma, no correlation was observed between the concentration of total proteins in seminal plasma and their ability to stimulate the secretion of CCL20 by HEC-1 cells. In contrast, the ability to produce CCL20 by HEC-1A cells correlated to the concentration of lactoferrin in the seminal plasma samples(r coefficient = 0.56; CI: 0.26-0.76; P < 0.001). After fractionation by ion exchange chromatography, the seminal plasma fractions exhibiting the highest concentrations of lactoferrin were responsible for the greatest stimulation of CCL20 production by HEC-1A cells(r coefficient = 0.89; CI: 0.78-0.95; P <0.0001). CONCLUSION: Lactoferrin present in seminal plasma correlated with an increased production of CCL20 by HEC-1A cells and therefore could facilitate HIV entry through the genital mucosa. | Alan Grupioni Loureno Marilena Chinalli Komesu Alcyone Artioli Machado Silvana Maria Quintana Thomas Bourlet Bruno Pozzetto Olivier Delézay | 2014 | World Journal of Virology2014，3，2： | 0 |
| 14 | Radiographic and tomographic description of marlin sucker Remora osteochir, Pisces: Echeneidae—preliminary data of one specimen显示文摘Remoras are commensal fish of various marine species,such as sharks,swordfishes,turtles,dolphins,manta rays and whales.One specimen of marlin sucker Remora osteochir was evaluated using computed tomography for skeletal digital reconstruction,digital radiology for general evaluation and bone counting,and the double contrast technique to distinguish coelomic organs.In radiographic images,it was possible to observe otoliths in the center of the neurocranium,to count 27 vertebrae,nine pairs of ribs,and to detect the presence of the hipural and epural bones near of the caudal fin.In double contrast images,it was possible to visualize the swim bladder,gills,heart,liver,stomach and intestines.From the tomographic images and reconstructions,it was possible to identify the intercalar bones of the cephalic disc;the spine with vertebral bodies composed of neural arches and ventral ribs;and the pectoral girdle formed by posttemporal,cleithrum and scapulocoracoid bones.It was concluded that digital radiology and computed tomography were able to describe anatomical structures of marlin sucker R.osteochir without the need for dissection. | AndréLuiz Veiga Conrado Renata Stecca lunes Carlos Eduardo Malavasi Bruno Aline Tiemi Shiraishi Rocha JoséRoberto Machado Cunha da Silva | 2020 | Marine Life Science & Technology2020，2，3： | 0 |
| 15 | Maternal hyperthyroidism increases the synthesis activity and the osteogenic markers expression of calvarial osteoblasts from offspring in a murine model显示文摘To evaluate the characteristics and synthesis activity of osteoblasts extracted from the calvaria of offspring of rats exposed to maternal hyperthyroidism.Twelve adult Wistar rats were divided into two groups,one control and one treated with daily administration of L-thyroxine by an orogastric tube(50μg/animal/day)during pregnancy.Three days after delivery and confirmation of the mothers’hyperthyroidism,the offspring were euthanized for the extraction of osteoblasts from the calvaria.At 7,14,and 21 days,proliferation activity was assessed using MTT assay,while alkaline phosphatase(ALP)activity was assessed by the BCIP/NBT method.At 21 days,the total area of the mineralized matrix stained by von Kossa was evaluated by morphometry.The expression of gene transcripts for Runx2,Bmp2,Fgfr1,collagen type 1(Col1),osteocalcin(Oc),and osteopontin(Op)were evaluated by real-time RT-PCR.Means were compared using the Student’s t-test.FA activity was significantly higher at 14 and 21 days in cultures of osteoblasts extracted from offspring exposed to maternal hyperthyroidism,while MTT conversion was significantly lower at 21 days in this group.Osteoblast cultures of neonates exposed to maternal hyperthyroidism also showed a larger total area of mineralized matrix and greater expression of gene transcripts for Oc and Op.Maternal hyperthyroidism increases the activity of matrix synthesis,alkaline phosphatase activity,and expression of gene transcripts for osteocalcin and osteopontin in the osteoblasts,extracted from the calvaria of the offspring,which may be one of the mechanisms of premature fusion of cranial sutures. | FABIANA ROCHA ARAÚJO BRUNO MACHADO BERTASSOLI ISABELLA CRISTINA SOUZA FÉLIX DOUGLAS MARINHO ABREU NATÁLIA MELO OCARINO AMANDA MARIA SENA REIS JUNEO FREITAS SILVA ROGÉRIA SERAKIDES | 2023 | BIOCELL2023，47，2： | 0 |
| 16 | Mechanical Circulatory Support in Patients with Sepsis: A Case Series显示文摘 | Helmgton Jose Brito de Souza Henrique Louzan Machado Thiago do Amaral Cavalcante Leonardo Jadyr Santos Rodrigues Alves Diogo Assis Souza Rafael Ramos Amaral Felipe Bruno Santos da Cunha Marcus Vinicius Nasimento dos Santos Glauco Kalil da Silva Pina Isaac Azevedo Silva Ricardo Borros Corso | 2018 | Journal of Pharmacy and Pharmacology2018，6，5： | 0 |
| 17 | Trace metal dynamics in an industrialized Brazilian river:A combined application of Zn isotopes,geochemical partitioning,and multivariate statistics显示文摘The Paraiba do Sul(PSR)and Guandu Rivers(GR)water diversion system(120 km long)is located in the main industrial pole of Brazil and supplies drinking water for 9.4 million people in the metropolitan region of Rio de Janeiro.This study aims to discern the trace metals dynamics in this complex aquatic system.We used a combined approach of geochemical tools such as geochemical partitioning,Zn isotopes signatures,and multivariate statistics.Zinc and Pb concentrations in Suspended Particulate Matter(SPM)and sediments were considerably higher in some sites.The sediment partition of As,Cr,and Cu revealed the residual fraction(F4)as the main fraction for these elements,indicating low mobility.Zinc and Pb were mostly associated with the exchangeable/carbonate(F1)and the reducible(F2)fractions,respectively,implying a higher susceptibility of these elements to being released from sediments.Zinc isotopic compositions of sediments and SPM fell in a binary mixing source process between lithogenic(δ^(66/64)Zn_(JMC)≈+0.30‰)and anthropogenic(δ^(66/64)Zn_(JMC)≈+0.15‰)end members.The lighterδ^(66/64)Zn_(JMC)values accompanied by high Zn concentrations in exchangeable/carbonate fraction(ZnF1)enable the tracking of Zn anthropogenic sources in the studied rivers.Overall,the results indicated that Hg,Pb,and Zn had a dominant anthropogenic origin linked to the industrial activities,while As,Cr,and Cu were mainly associated with lithogenic sources.This work demonstrates how integrating geochemical tools is valuable for assessing geochemical processes and mixing source effects in anthropized river watersheds. | Myller S.Tonhá Daniel F.Araújo Rafael Araújo Bruno C.A.Cunha Wilson Machado Joelma F.Portela João PR Souza Hikari K.Carvalho Elton L.Dantas Henrique L.Roig Patrick Seyler Jeremie Garnier | 2021 | Journal of Environmental Sciences2021，33，3： | 0 |
| 18 | Predicting major adverse cardiovascular events after orthotopic liver transplantation using a supervised machine learning model:A cohort study显示文摘BACKGROUND Liver transplant(LT)patients have become older and sicker.The rate of post-LT major adverse cardiovascular events(MACE)has increased,and this in turn raises 30-d post-LT mortality.Noninvasive cardiac stress testing loses accuracy when applied to pre-LT cirrhotic patients.AIM To assess the feasibility and accuracy of a machine learning model used to predict post-LT MACE in a regional cohort.METHODS This retrospective cohort study involved 575 LT patients from a Southern Brazilian academic center.We developed a predictive model for post-LT MACE(defined as a composite outcome of stroke,new-onset heart failure,severe arrhythmia,and myocardial infarction)using the extreme gradient boosting(XGBoost)machine learning model.We addressed missing data(below 20%)for relevant variables using the k-nearest neighbor imputation method,calculating the mean from the ten nearest neighbors for each case.The modeling dataset included 83 features,encompassing patient and laboratory data,cirrhosis complications,and pre-LT cardiac assessments.Model performance was assessed using the area under the receiver operating characteristic curve(AUROC).We also employed Shapley additive explanations(SHAP)to interpret feature impacts.The dataset was split into training(75%)and testing(25%)sets.Calibration was evaluated using the Brier score.We followed Transparent Reporting of a Multivariable Prediction Model for Individual Prognosis or Diagnosis guidelines for reporting.Scikit-learn and SHAP in Python 3 were used for all analyses.The supplementary material includes code for model development and a user-friendly online MACE prediction calculator.RESULTS Of the 537 included patients,23(4.46%)developed in-hospital MACE,with a mean age at transplantation of 52.9 years.The majority,66.1%,were male.The XGBoost model achieved an impressive AUROC of 0.89 during the training stage.This model exhibited accuracy,precision,recall,and F1-score values of 0.84,0.85,0.80,and 0.79,respectively.Calibration,as assessed by the Brier score,indicated excellent model calibration with a score of 0.07.Furthermore,SHAP values highlighted the significance of certain variables in predicting postoperative MACE,with negative noninvasive cardiac stress testing,use of nonselective beta-blockers,direct bilirubin levels,blood type O,and dynamic alterations on myocardial perfusion scintigraphy being the most influential factors at the cohort-wide level.These results highlight the predictive capability of our XGBoost model in assessing the risk of post-LT MACE,making it a valuable tool for clinical practice.CONCLUSION Our study successfully assessed the feasibility and accuracy of the XGBoost machine learning model in predicting post-LT MACE,using both cardiovascular and hepatic variables.The model demonstrated impressive performance,aligning with literature findings,and exhibited excellent calibration.Notably,our cautious approach to prevent overfitting and data leakage suggests the stability of results when applied to prospective data,reinforcing the model’s value as a reliable tool for predicting post-LT MACE in clinical practice. | Jonathan Soldera Leandro Luis Corso Matheus Machado Rech Vinícius Remus Ballotin Lucas Goldmann Bigarella Fernanda Tomé Nathalia Moraes Rafael Sartori Balbinot Santiago Rodriguez Ajacio Bandeira de Mello Brandão Bruno Hochhegger | 2024 | World Journal of Hepatology2024，16，2： | 0 |
| 19 | 混凝土“方体”里的休闲中心——巴西新利马度假养生会所显示文摘项目坐落于私人养生会所度假区域的中心地带,在这个倾斜的山坡上分散着一系列平房和酒店基础设施,项目俯瞰新利马,距离贝洛哈里桑塔市12公里.项目由两栋简单建筑物组成,中间是露天开放区.BCMF Architects做酒吧设计,MACh Architects做画廊设计,开放区域(游泳池和休息平台)由两个工作室合作设计,两个工作室自2010年就是关系密切的合作伙伴,并共享办公空间. | Bruno Campos Marcelo Fontes SilvioTodeschi Fernando Maculan Mariza Machado Coelho Gabriel Castro Jomar Braganca | 2016 | 设计家2016，，1： | 0 |
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