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| 1 | 光敏剂间-四羟基苯二氢卟酚及其复合物对人T淋巴细胞白血病细胞的作用显示文摘目的 观察光敏剂间-四羟基苯二氢卟酚(mTHPC)及其复合物HSA-mTHPC对人T淋巴细胞白血病细胞(Jurkat)的作用,探讨人血白蛋白(HSA)纳米颗粒作为mTHPC载体的可行性.方法 利用流式细胞仪测定Jurkat细胞对mTHPC及HSA-mTHPC的摄取率,通过测定不同浓度mTHPC及HSA-mTHPC孵育Jurkat细胞后的存活率、细胞膜完整性和增殖率,评价两种药物的摄取率和细胞毒性.结果 浓度1.0 mg/L,孵育4、24 h Jurkat细胞的摄取率为:mTHPC单体:98.07%、99.07% HSA-mTHPG中mTHPC:98.13%、99.19% HSA单体:2.77%、4.84%.光照后Jurkat细胞活性和增殖率呈显著剂量、时间依赖的下降,mTHPC浓度为1.0 mg/L时,4、24、48 h存活率和增殖率分别为13.27%、5.46%、4.99%和2.68%、0.91%、0.62%,HSA-mTHPC对细胞生长和增殖的抑制与mTHPC单体比较未见下降(P>0.05).光照后LDH活性呈现显著剂量、时间依赖的上升,mTHPC浓度为1.0mg/L时,4、24、48 h LDH活性分别为:80.99%、99.38%、100.67%.结论 mTHPC是一种杀伤力强的光敏剂型,能有效进入肿瘤细胞,并导致细胞损害.HSA纳米颗粒不影响细胞对mTHPC的摄取,可以作为其载体. | 郑顺贞 Karin Low Matthis Wacker Arno Wiehe Sylvia Wagner Klaus Langer Hagen von Briesen 邹声泉 | 2010 | 中华实验外科杂志2010，27，10： | 2 |
| 2 | Modular dynamic simulation for integrated particulate processes by means of tool integration 显示文摘 | Viateheslav Kulikov Heiko Briesen Robert Grosch Aidong Yang Lars von Wedel Wolfgang Marquardt | 2005 | Chemical Engineering Science2005，60，7： | 1 |
| 3 | Gelatin nanoparticles by two step desol- vation a new preparation method, surface modifications and cell uptake显示文摘 | Coester C J Langer K Von Briesen H | 2000 | Journal of microencapsulation2000，17，2： | 1 |
| 4 | Temperature fluctuations during deep temperature cryopreservation reduce PBMC recovery, viability and T-cell function显示文摘 | Anja Germann Young-Joo Oh Tomm Schmidt Uwe Sch?n Heiko Zimmermann Hagen von Briesen | 2013 | Cryobiology2013，，： | 1 |
| 5 | Preparation of avidin-labeled protein nanoparticles as carriers for biotinylated peptide nucleic acid (PNA) 显示文摘 | Langer K Coester C von Briesen H etal | 2000 | Eur J Pharm Biopharm2000，49，3： | 1 |
| 6 | Analysis of FBRM measure- ments by means of a 3D optical model 显示文摘 | Kail N Briesen H Marquardt W | 2008 | Powder Technology2008，185，3： | 1 |
| 7 | Humanserum albumin -polyethyleneimin nanoparticles for gene delivery显示文摘 | Rhase S von Briesen H Rubsamen-Waigmann H ei al | 2003 | Journal of Controlled Release2003，92，12： | 1 |
| 8 | Complication cascade after hyperopic LASIK显示文摘 | Handzel DM Stanzel BV Briesen S | 2011 | Ophthalmologe2011，108，7： | 1 |
| 9 | Gelatin Nanoparticles by Two Step Desolvation -- a New Preparation Method, Surface Modifications and Cell Uptake 显示文摘 | Coester C J Langer K yon Briesen H | 2000 | Journal of Microencapsulation2000，17，2： | 1 |
| 10 | Isolation and molecular characterization of brain microvascular endothelial cells from human brain tumors显示文摘 | Unger RE Oltrogge JB Von Briesen H | 2002 | In Vitro Cell Dev Biol Anim2002，38，： | 1 |
| 11 | Gelatin nanoparticles by two step desolvation: A new preparation method, surface modifications and cell uptake 显示文摘 | COESTER C J LANGER K VON BRIESEN H | 2000 | Journal of Microencapsulation2000，17，2： | 1 |
| 12 | Oil and gas produced water managementand surface drinking water sources in Pennsylvania 显示文摘 | Wilson J M Van Briesen J M | 2012 | EnvironmentalPractice2012，14，： | 1 |
| 13 | New approach to refinery process simulation with adaptive composition representation 显示文摘 | Briesen H Marquardt W | 2004 | AIChE Journal2004，50，3： | 1 |
| 14 | Cytotoxicity of triptolide and triptolide loaded polymeric micelles in vitro 显示文摘 | Zheng SZ Low K Wagner S Yang XL Briesen H Zou SQ | 2011 | Toxicol Vitro2011，25，8： | 1 |
| 15 | Gelatin nanoparticles by two step desolvation a new preparation method,surface modifications and cell uptake 显示文摘 | Coester C J Langer K Von Briesen H | 2000 | Journal of microencapsulation2000，17，2： | 1 |
| 16 | Human serum albumin-polyethylenimine nanoparticles for gene delivery显示文摘 | Stephanie Rhaese Hagen yon Briesen Helga Rubsamen- Waigmann | 2003 | J Controled Release2003，92，： | 1 |
| 17 | Preparation of avidin-labeled gelatin nanoparticles as carriers for biotinylated peptide nucleic acid (PNA) 显示文摘 | Coester C Kreuter J von Briesen H | 2000 | Int J Pharm2000，196，2： | 1 |
| 18 | Direct Evidence That Polysorbate-80-Coated Poly(Butylcyanoacrylate) Nanoparticles Deliver Drugs to the CNS via Specific Mechanisms Requiring Prior Binding of Drug to the Nanoparticles显示文摘 | J?rg Kreuter Peter Ramge Valery Petrov Stefan Hamm Svetlana E. Gelperina Britta Engelhardt Renad Alyautdin Hagen von Briesen David J. Begley | 2003 | Pharmaceutical Research2003，，3： | 1 |
| 19 | Raman spectroscopy and chemical imaging for quantification of fil- tered waterborne bacteria显示文摘 | Escoriza M F van Briesen J M Vanbriesen J | 2006 | J Microbiol Methods2006，66，1： | 1 |
| 20 | Minimal cross-infection risk through ICARE rebound tonometer probes: a useful tool for IOP-screenings in developing countries显示文摘 | Briesen S Schulze SM Roberts H | 2010 | Eye (Lond)2010，24，7： | 1 |
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