维普中文期刊产品整合服务
9篇 您的检索式:作者名="Boeneman"
    题名 作者 年代 出处 被引量
1Sensing easpase 3activity with quantum dot-fluorescent protein assemblies显示文摘Boeneman K Mei BC Dennis AM 2009J Am Chem Soc2009,131,11:1
2Self-assembled quantum dot-sensitized multivalent DNA photonic wires显示文摘Boeneman K Prasuhn DE Blanco-Canosa JB 0,,51:1
3Improved Peptidyl Linkers for Self-Assembly of Semiconductor Quantum Dot Bioconjugates显示文摘We demonstrate improved peptide linkers which allow both conjugation to biomolecules such as DNA and self-assembly with luminescent semiconductor quantum dots.A hexahistidine peptidyl sequence was generated by standard solid phase peptide synthesis and modified with the succinimidyl ester of iodoacetamide to yield a thiol-reactive iodoacetyl polyhistidine linker.The reactive peptide was conjugated to dye-labeled thiolated DNA which was utilized as a model target biomolecule.Agarose gel electrophoresis and fluorescence resonance energy transfer analysis confirmed that the linker allowed the DNA to self-assemble with quantum dots via metal-affinity driven coordination.In contrast to previous peptidyl linkers that were based on disulfide exchange and were thus labile to reduction,the reactive haloacetyl chemistry demonstrated here results in a more stable thioether bond linking the DNA to the peptide which can withstand strongly reducing environments such as the intracellular cytoplasm.As thiol groups occur naturally in proteins,can be engineered into cloned proteins,inserted into nascent peptides or added to DNA during synthesis,the chemistry demonstrated here can provide a simple method for self-assembling a variety of stable quantum dot bioconjugates.Lorenzo Berti Paola Serena D'Agostino Kelly Boeneman Igor L.Medintz 2009Nano Research2009,2,2:1
4Sensing caspase 3 activity with quantum dot-fluorescent protein assemblies显示文摘Boeneman K Mei BC Dennis AM 2009J Am Chem Soc2009,131,11:1
5Sensing Caspase 3 activity with quantum dot-fluorescent protein American Chemical Society, 2009 assemblies 显示文摘BOENEMAN K MEI B C DENNIS A M BAO G DESCHAMPS J R MATTOUSSI H MED1NTZ I L 2009Journal of the2009,131,11:1
6Sensing caspase 3activity with quantum dot-fluorescent protein assemblies显示文摘Boeneman K Mei BC Dennis AM 2009J Am Chem Soc2009,131,11:1
7Cytotoxicity of quantum dots used/br in vitro cellular labeling: role of QD surface ligand, delivery modality, cell type and direct comparison to organic fluorophores 显示文摘Bradburne C E Delehanty J B Boeneman G K 2013Bioconjugate Chemistry2013,24,9:1
8Delivering quantum dot peptide bioconjugates to the cellular cytosol:escaping from the endolysosomal system显示文摘Delehanty J B Bradburne C E Boeneman K 0,,5:1
9Quan- tum dots and fluorescent protein FRET-based biosensors显示文摘BOENEMAN K DELEHANTY JB SUSUMU K 2012Adv Exp Med Biol2012,733,:1
返回顶部 每页显示:
共1页 首页 上一页 第1页 下一页 末页 /1 跳转

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1 渝公网安备 50019002500403号 违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费