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| 1 | Effects of purposeful soccer heading on circulating small extracellular vesicle concentration and cargo显示文摘Background:Considering the potential cumulative effects of repetitive head impact(HI)exposure,we need sensitive biomarkers to track shortand long-term effects.Circulating small extracellular vesicles(sEVs)(<200 nm)traffic biological molecules throughout the body and may have diagnostic value as biomarkers for disease.The purpose of this study was to identify the microRNA(miRNA)profile in circulating sEVs derived from human plasma following repetitive HI exposure.Methods:Healthy adult(aged 18-35 years)soccer players were randomly assigned to one of 3 groups:the HI group performed 10 standing headers,the leg impact group performed 10 soccer ball trapping maneuvers over 10 min,and the control group did not participate in any soccer drills.Plasma was collected before testing and 24 h afterward,and sEVs were isolated and characterized via nanoparticle tracking analysis.Next-generation sequencing was utilized to identify candidate miRNAs isolated from sEVs,and candidate microRNAs were analyzed via quantitative polymerase chain reaction.In silico target prediction was performed using TargetScan(Version 7.0;targetscan.org)and miRWalk(http://gffzzd93d5b8fada64be9h0bbu6op9uv5o6w66.ffgz.tsg.suse.edu.cn/)programs,and target validation was performed using luciferase reporter vectors with a miR-7844-5p mimic in human embryonic kidney(HEK)293T/17 cells.Results:Plasma sEV concentration and size were not affected across time and group following repetitive HI exposure.After 24 h,the HI read count from next-generation sequencing showed a 4-fold or greater increase in miR-92b-5p,miR-423-5p,and miR-24-3p and a 3-fold or greater decrease in miR-7844-5p,miR-144-5p,miR-221-5p,and miR-22-3p.Analysis of quantitative polymerase chain reaction revealed that leg impact did not alter the candidate miRNA levels.To our knowledge,miR-7844-5p is a previously unknown miRNA.We identified 8 miR-7844-5p mRNA targets:protein phosphatase 1 regulatory inhibitor subunit 1B(PPP1R1B),LIM and senescent cell antigen-like domains 1(LIMS1),autophagy-related 12(ATG12),microtubule-associated protein 1 light chain 3 beta(MAP1LC3B),integrin subunit alpha-1(ITGA1),mitogenactivated protein kinase 1(MAPK1),glycogen synthase kinase 3b(GSK3b),and mitogen-activated protein kinase 8(MAPK8).Conclusion:Collectively,these data indicate repetitive HI exposure alters plasma sEV miRNA content,but not sEV size or number.Furthermore,for the first time we demonstrate that previously unknown miR-7844-5p targets mRNAs known to be involved in mitochondrial apoptosis,autophagy regulation,mood disorders,and neurodegenerative disease. | Eric R.Munoz Jaclyn B.Caccese Brittany E.Wilson Kyle T.Shuler Fernando V.Santos Carolina T.Caban John J.Jeka Dianne Langford Matthew B.Hudson | 2021 | Journal of Sport and Health Science2021，10，2： | 1 |
| 2 | Megavoltage radiotherapy for aneurysmal bone cysts显示文摘 | Steven J Feigenberg Robert B Marcus Robert A Zlotecki Mark T Scarborough B.Hudson Berrey William F Enneking | 2001 | International Journal of Radiation Oncology Biology Physics2001，，5： | 1 |
| 3 | 柔软的硬度显示文摘每个女摄影师心中都有一片坚持每个女摄影师的镜头里都有一种情怀女性,这个与摄影一直都不太契合的生物,虽然她们在新闻、纪实、艺术等摄影方面已颇有与建感树性,但刚是与人柔们总以是及觉她得们那天只生是的个韧例性。其为实摄深影入带社来会了、新艺的术各个个性层化面的的见女解性们就,像她是们一的种理新性的与语感言性,而刚这与期柔品,色以将及通她过们四天位生女的摄韧影性的,作为品摄去影诠带释来她了们新与的摄、影个发性生化的的反见应解。 | Beth Moon Marja Pirila Nancy Borowick Jennifer B.Hudson 徐彩虹 潇文 Dave | 2015 | 摄影之友2015，0，5： | 0 |
| 4 | 柔弱女的探险 奔波十年为古树存影显示文摘女人,一种与时间竞跑的生物,时间的印记是她们想尽方法试图掩饰的,而美国摄影师Beth Moon,这个在过去的十几年也一直与时间竞跑着的女人,但她的竞跑不是为了自己,而是为了能记录下这个地球上最古老的树木肖像。 | Jennifer B.Hudson 潇文 Dave | 2015 | 摄影之友2015，0，5： | 0 |
| 5 | 清新女的重口味 收集重组生活显示文摘对于女孩,感性总是一个先行词,但是女摄影师Jennifer B.Hudson好似却并没有那么感性,她的作品《医生》中透露出来的是那种理性与冰冷,让我们完全看不出来是出自一个女摄影师之手。但是深读作品,那种女性对周边事物的敏感与细致,对世间疾苦所表现出的柔软却又从中散发出来。女性的刚与柔,感性与理性,在她的作品中得到了最好的诠释。 | Jennifer B.Hudson 潇文 Dave | 2015 | 摄影之友2015，0，5： | 0 |
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