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您的检索式：作者名="Azadeh Hatef"
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| 1 | Kisspeptin stimulates oocyte maturation,and food deprivation modulates the abundance of kisspeptin system in zebrafish gonads显示文摘Kisspeptin is a hormone involved in the neuroendocrine control of reproduction in fish.We hypothesized that kisspeptin stimulates oocyte maturation and modulates other reproductive hormones in zebrafish;and the gonadal kisspeptin system in zebrafish is affected during energy unavailability.The main goals of this research were to test in vitro effects of kisspeptin on oocyte maturation and mRNA abundance in zebrafish ovarian follicles and determine how short-term feed restriction affects kisspeptin and its receptors in zebrafish testis and ovary.This study demonstrates the presence of kisspeptin and its receptors in zebrafish(Danio rerio)gonads and its direct action on ovarian follicles in vitro.Kisspeptin(10 ng/mL)induced oocyte maturation,as indicated by germinal vesicle break down at 18-and 24-hours post-treatment.Kisspeptin significantly increased the abundance of mRNAs encoding reproductive hormones and its receptors in zebrafish oocytes.This suggests that kisspeptin-10 affects ovarian functions by modulating other hormones.Reproduction is a process that requires energy.Therefore,whether energy availability affects the kisspeptin system in zebrafish gonads was determined.Food deprivation modulated kisspeptin expression differently in zebrafish testis and ovary.Kiss2 and kiss1ra were upregulated while kiss1rb was downregulated in the testis post-food deprivation.Meanwhile,no changes in kiss in the ovary were found after food deprivation.However,kiss1rb was downregulated in unfed fish at 3-and 7-days post-food deprivation.Overall,our results suggest sex-and tissue-specific changes in the gonadal abundance of the kisspeptin system in zebrafish.The fine tuning of reproduction during energy fluctuations in fish is likely mediated via changes in hormones,including kisspeptin as shown in this research. | Azadeh Hatef Jithine Jayakumar Rajeswari Suraj Unniappan | 2022 | Aquaculture and Fisheries2022，7，5： | 0 |
| 2 | The ghrelinergic system in zebrafish gonads is suppressed during food unavailability显示文摘Ghrelin is an appetite stimulatory peptide that regulates reproduction in vertebrates.The acylation of ghrelin by ghrelin-O-acyltransferase(Goat)is essential for most of its functions.The ghrelinergic system(ghrelin,ghrelin receptor[Ghsr]and Goat)is present in teleost fish ovary and testis.In this research,we determined the abundance of the ghrelinergic system mRNAs in different stages of follicle(oocyte)development in zebrafish ovary.The highest levels of preproghrelin mRNA were observed in the first stage of follicular development(previtellogenesis).Significantly low levels of preproghrelin mRNA were observed in stages 3II,4 and 5 of the follicle development.Ghsr mRNA abundance remained unchanged at early stages of follicle development and decreased in stage 5 compared to other developmental stages.A significant decrease in preproghrelin and Ghsr mRNAs in the testis,and ghsr mRNA in the ovary of zebrafish deprived of food for 3 days were found.At 7 days food deprivation,preproghrelin,ghsr and goat mRNA in the ovary and preproghrelin and goat in the testis of zebrafish were significantly reduced.These results show that metabolic status modulates gonadal ghrelinergic system in zebrafish and provide support for a role for ghrelin in fish reproduction. | Azadeh Hatef Jithine Jayakumar Rajeswari Suraj Unniappan | 2022 | Aquaculture and Fisheries2022，7，5： | 0 |
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