|
|
|
题名
|
作者
|
年代
|
出处
|
被引量
|
| 1 | Portal vein thrombosis:Prevalence,patient characteristics and lifetime risk:A population study based on 23796 consecutive autopsies显示文摘瞄准:估计一生在一般人口的门静脉血栓形成(PVT ) 的累积发生。方法:在 1970 和 1982 之间, 23,796 具尸体,在马尔摩城市人口代表 84% 所有在里面医院死亡,被执行,用包括门静脉的检查的一个 standardised 协议。PVT 病人被描述并且在尸体的 PVT 流行,一生的表情累积发生,在高风险的疾病范畴估计了并且以机会比率和 95% CI 表示了。结果:PVT 的人口流行是 1.0% 。有 PVT 28% 的 254 个病人有肝硬化, 23% 主要并且 44% 第二等的肝胆管恶意, 10% 主要腹的传染或煽动性的疾病并且 3% 有 myeloproliferative 混乱。有肝硬化和肝的癌的病人有最高的 PVT 风险,或 17.1 (95% CI 11.1-26.4 ) 。在 14% ,没有原因被发现;仅仅,少数他们开发了 portal-hypertension-related 复杂并发症。结论:在这基于人口的研究, PVT 比由以前的临床的系列显示被发现更普通。显著地,在肝硬化和肝的癌的过量风险应该在指导干预的未来的研究可能为被考虑的这些病人保证增加的了解。 | Mats gren David Bergqvist Martin Bjrck Stefan Acosta Henry Eriksson Nils H Sternby | 2006 | World Journal of Gastroenterology2006，12，13： | 66 |
| 2 | Surgical management of peritonitis secondary to acute superior mesenteric artery occlusion显示文摘Diagnosis of acute arterial mesenteric ischemia in the early stages is now possible using modern computed tomography with intravenous contrast enhancement and imaging in the arterial and/or portal phase.Most patients have acute superior mesenteric artery(SMA)occlusion,and a large proportion of these patients will develop peritonitis prior to mesenteric revascularization,and explorative laparotomy will therefore be necessary to evaluate the extent and severity of intestinal ischemia,and to perform bowel resections.The establishment of a hybrid operating room in vascular units in hospitals is most important to be able to perform successful intestinal revascularization.This review outlines current frontline surgical strategies to improve survival and minimize bowel morbidity in patients with peritonitis secondary to acute SMA occlusion.Explorative laparotomy needs to be performed first.Curative treatment is based upon intestinal revascularization followed by bowel resection.If no vascular imaging has been carried out,SMA angiography is performed.In case of embolic occlusion of the SMA,open embolectomy is performed followed by angiography.In case of thrombotic occlusion,the occlusive lesion can be recanalized retrograde from an exposed SMA,the guidewire snared from either the femoral or brachial artery,and stented with standard devices from these access sites.Bowel resections and sometimes gall bladder removal due to transmural infarctions are performed at initial laparotomy,leaving definitive bowel reconstructions to a planned second look laparotomy,according to the principles of damage control surgery.Patients with peritonitis secondary to acute SMA occlusion should be managed by both the general and vascular surgeon,and a hybrid revascularization approach is of utmost importance to improve outcomes. | Stefan Acosta | 2014 | World Journal of Gastroenterology2014，20，29： | 14 |
| 3 | Original article Schistosomiasis in China: acute infections during 2005-2008显示文摘背景重要进步在控制发生在过去的 50 年血吸虫病在中国的装饰用的梨树。然而,可得到的数据建议了那血吸虫病立即在从 1992 ~ 2001 被进行的世界银行贷款工程以后有 re-emerged。有到控制的修订策略的国家控制程序由使用综合措施血吸虫病自从 2005,被实现了。在这研究,我们试图评估国家程序的效果在上血吸虫病从 2005 ~ 2008 .Methods A 的控制回顾的学习被执行与血吸虫 japonicum 分析急性感染的流行模式(S。japonicum ) ,从 564, 207, 83 和 57 个尖锐案例的 2005 ~ 2008 .Results A 总数基于每年从基于万维网的国家传染性的疾病收集的尖锐案例的数字报导系统与 S 感染了。分别地, japonicum 与 46.35% 的平均年度减小率在 2005, 2006, 2007 和 2008 全国被报导在期间持续四年。有 S 的急性感染的六爆发。japonicum 在 2005 被报导但是没有在 2006 ~ 2008 的时期。被报导的所有尖锐案例主要来自湖区域并且变得从第 27 在更高的风险时期期间感染了到第 43 星期一年。大多数这些案例是学生(44.87%) ,农民(31.51%) 和走水路得了感染的渔民(7.79%) 主要通过游泳联系(41.49%) 并且生产活动(40.25%) 。与时间,在所有尖锐案例之中的进口案例的比例由于与更活动的 population.Conclusions 发生了的人的更多的经常的运动增加了国家控制程序在上血吸虫病有修订控制策略的 aliened 有效地被带进效果。然而,仍然有在在湖区域的学生,农民和渔民生活之中的感染的重要风险。因此,在传播,或湖区域的高风险区域在风险人口之中加强控制措施是重要的。 | LI Shi-zhu Acosta Luz WANG Xian-hong XU Li-li WANG Qiang QIAN Ying-jun WU Xiao-hua GUO Jia-gang XIA Gang WANG Li-ying ZHOU Xiao-nong | 2009 | Chinese Medical Journal2009，，9： | 13 |
| 4 | Inflammatory and oxidative stress in rotavirus infection显示文摘Rotaviruses are the single leading cause of life-threatening diarrhea affecting children under 5 years of age. Rotavirus entry into the host cell seems to occur by sequential interactions between virion proteins and various cell surface molecules. The entry mechanisms seem to involve the contribution of cellular molecules having binding, chaperoning and oxido-reducing activities. It appears to be that the receptor usage and tropism of rotaviruses is determined by the species, cell line and rotavirus strain. Rotaviruses have evolved functions which can antagonize the host innate immune response, whereas are able to induce endoplasmic reticulum(ER) stress, oxidative stress and inflammatory signaling. A networking between ER stress, inflammation and oxidative stress is suggested, in which release of calcium from the ER increases the generation of mitochondrial reactive oxygen species(ROS) leading to toxic accumulation of ROS within ER and mitochondria. Sustained ER stress potentially stimulates inflammatory response through unfolded protein response pathways. However, the detailed characterization of the molecular mechanisms underpinning these rotavirus-induced stressful conditions is still lacking. The signaling events triggered by host recognition of virusassociated molecular patterns offers an opportunity for the development of novel therapeutic strategies aimed at interfering with rotavirus infection. The use of N-acetylcysteine, non-steroidal anti-inflammatory drugs and PPARγ agonists to inhibit rotavirus infection opens a new way for treating the rotavirus-induced diarrhea and complementing vaccines. | Carlos A Guerrero Orlando Acosta | 2016 | World Journal of Virology2016，5，2： | 12 |
| 5 | 地理信息系统技术在伤寒发热监测中应用显示文摘目的运用地理信息系统(GIS)技术,了解各居民点发热流行率和评价以医疗机构为哨点的伤寒发热监测系统的敏感性。方法收集医学人口普查数据、疾病监测系统信息数据、现场采集居民居住点全球卫星定位数据等资料,建立GIS系统和以人口为基数的发热监测系统;对所有发热就诊病人进行身份和居住地址确认,并制作各居民点年发热就诊率GIS分布图。结果共采集3776个居民居住的全球定位系统(GPS)定位点,人群发热就诊率为1.11‰,发热就诊人次占总门诊人次的16.3%,监测区内的发热就诊病人占所有发热就诊病人的83.5%。在3种类型的医疗机构中,门诊量以个体门诊类最多,但其发热就诊人次占总门诊人次的比率最低(12.6%)。监测区内发热1d或以上的病人地址和身份(ADDRESS-ID)确认率达到95%。根据居民点年发热就诊率GIS分布图,发热就诊率最高的居民点达到17.81‰,最低为0;发热就诊率高的居民点多集中在城市。结论利用GIS技术可直观准确的了解疾病流行率的分布,可迅速直观的寻找出监测系统中的薄弱环节和推断出可能存在的问题。 | 杨进 董柏青 张杰 廖和壮 梁大斌 吴兴华 司国爱 杨宏徽 Jin Kyung Park R Leon Ochiai Camilo J Acosta Moharmmad Ali John D Clemens | 2007 | 中国公共卫生2007，23，9： | 8 |
| 6 | An Analysis of the Inverse Kinematics for a 5-DOF Manipulator显示文摘This paper proposes an analytical solution for a 5-DOF manipulator to follow a given trajectory while keeping the orientation of one axis in the end-effector frame. The forward kinematics and inverse kinematics for a 5-DOF manipulator are analyzed systemically. The singular problem is discussed after the forward kinematics is provided. For any given reachable position and orientation of the end-effector, the derived inverse kinematics will provide an accurate solution. In other words, there exists no singular problem for the 5-DOF manipulator, which has wide application areas such as welding, spraying, and painting. Experiment results verify the effectiveness of the methods developed in this paper. | Carlos A. Acosta Calderon John Q. Gan | 2005 | International Journal of Automation and computing2005，2，2： | 7 |
| 7 | 暴露在加勒比海水中的2219-T42和6061-T6铝合金表面初始局部腐蚀行为的比较(英文)显示文摘将6061-T6和2219-T42铝合金样品暴露在加勒比海水中90 d。将开路电位的波动作为电化学噪声(EN),用来表征和比较合金表面的初始点蚀行为。采用功率谱密度(PSD)与频率的关系图进行电化学噪声分析。PSD图中指数β的减小表明,随着海水中铝合金点蚀的进行,出现自发的能量释放。开路电位的波动与新腐蚀层的破裂和形成有关。PSD图中指数β的变化趋势表明,AA2219-T42铝合金的腐蚀过程是一个持续的非稳态过程,其动力学行为受分形布朗运动(fBm)控制;AA6061-T6铝合金的腐蚀过程则主要表现出稳态和弱持久性的特性,这是由于分形高斯噪声(fGn)的贡献。将合金暴露于海水中后进行扫描电镜-X射线能谱(SEM-EDX)分析,发现合金上存在富含Cu或Fe、不规则分布的不溶性金属间化合物颗粒。AA2219-T42合金中Mg和Al的优先溶解发生在S相(Al_2CuMg)。 | Gloria ACOSTA Lucien VELEVA Juan Luis LóPEZ D.A.LóPEZ-SAURI | 2019 | Transactions of Nonferrous Metals Society of China2019，29，1： | 7 |
| 8 | 5~60岁人群伤寒Vi多糖疫苗和A群脑膜炎球菌多糖疫苗大规模接种结果分析显示文摘为考核国产伤寒Vi多糖疫苗在现场试验的效果 ,以A群脑膜炎球菌多糖疫苗为对照 ,对广西壮族自治区河池市城市和农村的 5~ 6 0岁居民进行了一次大规模接种。结果显示 :应接种对象 1180 71人 ,实际接种 92 4 76人 ,接种率为 78 32 % ,其中伤寒Vi多糖疫苗为 76 87% ,A群脑膜炎球菌多糖疫苗为 79 6 9% ;接种率最高的组群接种率为 92 4 8% ,最低为 6 5 2 0 %。按城乡划分 ,城区的接种率为 77 2 9% ,农村的接种率为 80 5 8% ,组群接种率的高低分布无明显的地理性差异。 5~ 9岁接种率最高为 89 6 5 % ,2 0~ 2 9岁接种率最低为 6 9 0 7% ;男女接种率分别为79 0 7%和 82 11%。有 6 6人被观察到或报告出现不良反应 ,不良反应发生率为 71 37/ 10万。表明两种疫苗的安全性好。在大规模疫苗接种中 ,充分发挥当地干部的宣传组织作用 ,并把接种的有关信息提前通知当地居民 ,对于提高接种率十分重要。补漏接种能使接种率低的组群得到显著的提高。在大规模疫苗接种中必须严格遵守安全注射操作程序 ,避免发生不安全注射事故。两种疫苗的平均接种率、年龄别接种率、性别接种率、组群接种率的地理分布均达到了较好均衡性 ,是可比的 。 | 杨进 司国爱 曾竣 张杰 梁贵臣 梁大斌 廖和壮 李翠云 黄焕新 董柏青 杨宏徽 Rion Leon Ochiai Carolina Danovaro Ali Mohammad Camilo J Acosta | 2004 | 中国计划免疫2004，10，4： | 6 |
| 9 | Enhancing endogenous stem cells in the newborn via delayed umbilical cord clamping显示文摘There is currently no consensus among clinicians and scientists over the appropriate or optimal timing for umbilical cord clamping. However, many clinical studies have suggested that delayed cord clamping is associated with various neonatal benefits including increased blood volume, reduced need for blood transfusion, increased cerebral oxygenation in pre-term infants, and decreased frequency of iron deficiency anemia in term infants. Human umbilical cord blood contains significant amounts of stem and progenitor cells and is currently used in the treatment of several life-threatening diseases. We propose that delayed cord clamping be encouraged as it enhances blood flow from the placenta to the neonate, which is accompanied by an increase supply of valuable stem and progenitor cells, as well as may improve blood oxygenation and increase blood volume, altogether reducing the infant's susceptibility to both neonatal and age-related diseases. | Christopher Lawton Sandra Acosta Nate Watson Chiara Gonzales-Portillo Theo Diamandis Naoki Tajiri Yuji Kaneko Paul R.Sanberg Cesar V.Borlongan | 2015 | Neural Regeneration Research2015，10，9： | 6 |
| 10 | Helicobacter pylori CagA protein polymorphisms and their lack of association with pathogenesis显示文摘AIM: To investigate Helicobacter pylori (H. pylori) CagA diversity and to evaluate the association between protein polymorphisms and the occurrence of gastric pathologies. METHODS: One hundred and twenty-two clinical isolates of H. pylori cultured from gastric biopsies obtained from Colombian patients with dyspepsia were included as study material. DNA extracted from isolates was used to determine cagA status, amplifying the C-terminal cagA gene region by polymerase chain reaction. One hundred and six strains with a single amplicon were sequenced and results were used to characterize the 3' variable region of the cagA gene. To establish the number and type of tyrosine phosphorylation motifs Glutamine acid-Proline-Isoleucine-Tyrosine-Alanine (EPI-YA) bioinformatic analysis using Amino Acid Sequence Analyzer-Amino Acid Sequence Analyzer software was conducted. Analysis of the association between the number of EPIYA motifs and the gastric pathology was performed using χ2 test and analysis of the presence of EPIYA-C motifs in relation to the pathology was made by logistic regression odds ratios. Comparisons among EPIYA types found and those reported in GenBank were performed using a proportion test in Statistix Analytical Software version 8.0. RESULTS: After amplification of the 3' of the cagA gene, 106 from 122 isolates presented a single amplicon and 16 showed multiple amplicons. As expected, diversity in the size of the cagA unique fragments among isolates was observed. The 106 strains that presented a single amplicon after 3' cagA amplification came from patients with gastritis (19 patients), atrophic gastritis (21), intestinal metaplasia (26), duodenal ulcer (22) and gastric cancer. DNA sequence analysis showed that the differences in size of 3' cagA unique fragments was attributable to the number of EPIYA motifs: 1.9% had two EPIYA motifs, 62.3% had three, 33.0% had four and 2.8% had five motifs. The majority of tested clinical strains (62.3%) were found to harbor the ABC combination of EPIYA motifs and a significant statistical difference was observed between the frequencies of ABCC tyrosine phosphorylation motifs and Western strains sequences deposited in GenBank. CONCLUSION: The present report describes a lack of association between H. pylori CagA-protein polymorphisms and pathogenesis. ABCC high frequency variations compared with Western-strains sequences deposited in GenBank require more investigation. | Nicole Acosta Andrés Quiroga Pilar Delgado María Mercedes Bravo Carlos Jaramillo | 2010 | World Journal of Gastroenterology2010，16，31： | 5 |
| 11 | Medication adherence in schizophrenia显示文摘Non-adherence is a major problem in the treatment of schizophrenia. Its high prevalence, potentially severe consequences and associated costs make the study of this phenomenon a priority issue. In this article, basic non-adherence concepts of prevalence, consequences, evaluation methods, methodological restrictions of available studies, risk factors and intervention strategies, are reviewed. Studying non-adherence risk factors is a necessary step toward designing adequately oriented intervention strategies. An operative definition of adherence and good knowledge of its evaluation methods are essential to study this phenomenon. Unfortunately, most available studies contain methodological restrictions, especially concerning the evaluation methods, and an agreed operative definition of adherence has only very recently been reached. Knowing non-adherence risk factors, intervention strategies and available evidence on their effectiveness is essential in making treatment decisions in daily clinical practice. | Francisco Javier Acosta José Luis Hernández José Pereira Judit Herrera Carlos J Rodríguez | 2012 | World Journal of Psychiatry2012，2，5： | 5 |
| 12 | Human papilloma virus and esophageal carcinoma in a Latin-American region显示文摘AIM:To investigate the presence of high-risk human papilloma virus (HPV) in esophageal squamous cell carcinomas (ESCCs) in a non-selected Mexican population.METHODS: Cases with a pathological diagnosis of squamous cell carcinoma of the esophagus were obtained from Department of Pathology files, at the National Cancer Institute in Mexico City during the period between 2000 and 2008. Slides from each case were reviewed and cases with sufficient neoplastic tissue were selected for molecular analysis. DNA was extracted from paraffin-embedded tissue samples for polymerase chain reaction analysis to detect HPV DNA sequences. Demographic and clinical data of each patient were retrieved from corresponding clinical records.RESULTS: HPV was detected in 15 (25%) of ESCCs. HPV-16 was the most frequently observed genotype, followed by HPV-18; HPV-59 was also detected in one case. Unfortunately, HPV genotype could not be established in three cases due to lack of material for direct sequencing, although universal primers detected the presence of HPV generic sequences. No low-risk HPV genotypes were found nor was HPV-16/18 co-infection. HPV presence in ESCC was not significantly associated with gender, age, alcohol consumption, smoking, anatomic location, or histologic grade. All patients belonged to low and very low socioeconomic strata, and were diagnosed at advanced disease stage. Male patients were most commonly affected and the male:female ratio in HPV-positive ESCC increased two- fold in comparison with HPV-negative cases (6.5:1 vs 3.1:1).CONCLUSION: High prevalence of high-risk HPV in ESCC in Mexico does not support the hypothesis that HPV-associated ESCC is more common in areas with higher ESCC incidence rates. | Roberto Herrera-Goepfert Marcela Lizano Suminori Akiba Adela Carrillo-García Mauricio Becker-D'Acosta | 2009 | World Journal of Gastroenterology2009，15，25： | 4 |
| 13 | 以人口为基数的伤寒发热监测结果分析显示文摘目的及时了解、掌握伤寒高发区流行情况。方法在广西河池市的金城江辖区内的金城江镇、东江镇建立以人口为基数的监测系统开展伤寒监测,监测内容包括对发热疑似病人进行病原学、血清学检测;对就诊发热病例进行登记。监测区内的各级医疗机构及大部分的个体诊所均作为监测点。以病原学为确诊依据,肥达试验作为辅助诊断依据。结果监测区内4年的伤寒副伤寒发病数为99例,年均年龄段发病率为20.6/10万。病原学监测9049份标本,分离出伤寒、副伤寒菌99株,其中甲型副伤寒沙门氏菌占70.1%,伤寒沙门氏菌仅占29.9%;伤寒副伤寒症状相似,以发热头痛为主要症状。结论监测区内以散发病例为主要流行趋势,监测的发病率低于以往的常规疫情报告,甲型副伤寒沙门菌是优势菌型;肥达试验在常规监测中的诊断意义较差,确诊伤寒、副伤寒病例的肥达试验诊断符合率低。 | 张杰 董柏青 杨进 王鸣柳 梁贵臣 司国爱 曾竣 谭冬梅 廖和壮 梁大斌 李翠云 吴兴华 RLeon Ochiai Jin-kyung Park Ali Mohammad Camilo J Acosta J.D.Clemens | 2006 | 应用预防医学2006，12，6： | 4 |
| 14 | Specific and cross-reactive immune response against Mycobacterium tuberculosis antigens in mice immunized with proteoliposomes from Mycobacterium bovis BCG显示文摘Objective: To characterize the immunogenicity and the induction of cross-reactive responses against Mycobacterium tuberculosis(M. tuberculosis) of a proteoliposome(PL)from Mycobacterium bovis Bacillus Calmette–Guerin(BCG) with and without alum hydroxide(AL) as adjuvant(PLBCG-AL and PLBCG, respectively) in BALB/c mice.Methods: BALB/c mice were inoculated with phosphate buffer solution, BCG, PLBCG and PLBCG-AL. The humoral immunogenicity was determined by ELISA [immunoglobulin G(Ig G), Ig G1 and Ig G2a] and the cellular immunogenicity was evaluated in vivo by delayed type hypersensitivity. The humoral cross-reactive response against M. tuberculosis was determined by Western blot.Results: Sera from animals immunized with PLBCG-AL and PLBCG showed significant increase in specific total Ig G and Ig G1 antibodies and the presence of cross-reactive antibodies against M. tuberculosis antigens, which were more intense with the use of alum as adjuvant. Mice immunized with PLBCG and PLBCG-AL also showed a specific cellular response in vivo.Conclusions: The cellular and humoral immunogenicity of PLBCG and the capacity to induce cross-reactive responses against M. tuberculosis is in agreement with the protective capacity previously demonstrated by this vaccine candidate and supports the continuation of its evaluation in further stages. | Nadine Alvarez DaymíSerpa Ramlah Kadir Yanely Tirado Reinier Borrero Sonsire Fernández Rubén Cabrera Yolanda Valdes Caridad Zayas Reinaldo Acevedo Luis Izquierdo María Elena Sarmiento Mohd-Nor Norazmi JoséLuis Pérez Armando Acosta | 2017 | Asian Pacific Journal of Tropical Biomedicine2017，7，3： | 3 |
| 15 | Re: Halmos et al, A Diet Low in FODMAPs Reduces Symptoms of Irritable Bowel Syndrome显示文摘 | Michael Camilleri Andres Acosta | 2014 | Gastroenterology2014，，7： | 3 |
| 16 | 口头尸检对河池市5~60岁死亡病例进行死因评估结果分析显示文摘目的分析河池市5~60岁居民死亡主要原因,分析口头尸检(VerbalAutopsy,VA)进行死因调查的效果。方法建立死亡监测系统,对所有报告的监测区内5~60岁死亡个案进行口头尸检调查。结果2002年7月—2003年6月金城江镇、东江镇共死亡624人,全人口总粗死亡率为4.67‰,标化死亡率为5.34‰,男女之比为1.71∶1;5~60岁死亡病例248例,粗死亡率为2.10‰;导致5~60岁居民主要死亡原因为意外死亡、恶性肿瘤、心脑血管疾病、消化系统疾病等,其中意外死亡占死亡构成比的1/3;通过VA进行死因调查,使3.00%的错误死因定义得到更正、5.00%的不明死因得到诊断;无因伤寒导致的死亡。结论交通事故成为当地5~60岁居民死亡的主要因素;随着社会经济发展,伤寒等肠道传染病已不是导致死亡的重要因素;使用VA对于死因的判定比一般的监测系统报告更为准确。 | 杨进 韦士良 刘莉莉 黄兰森 温贤莲 张杰 曾竣 董柏青 吴兴华 RLeon Ochiai Ali Mohammad Camilo J Acosta | 2004 | 中国初级卫生保健2004，18，6： | 3 |
| 17 | CagA EPIYA polymorphisms in Colombian Helicobacter pylori strains and their influence on disease-associated cellular responses显示文摘AIM: To investigate the influence of the CagA diversity in Helicobacter pylori (H. pylori ) strains from Colombia on the host cell biology. METHODS: Eighty-four H. pylori-cagA positive strains with different Glu-Pro-Ile-Tyr-Ala (EPIYA) motifs patterns, isolated from patients with gastritis (n=17), atrophic gastritis (n=17), duodenal ulcer (n=16), intestinal metaplasia (n=16) and gastric cancer (n=18), were included. To determine the integrity of the cag pathogenicity island (cag PAI) we evaluated the presence of cagA, cagT, cagE, and cag10 genes by polymerase chain reaction. AGS gastric epithelial cellswere infected with each strain and assayed for translo-cation and tyrosine phosphorylation of CagA by western blot, secretion of interleukin-8 (IL-8) by enzyme-linked immuno sorbent assay after taking supernatants from cocultures and cell elongation induction. For cell elongation quantification, coculture photographs were taken and the proportion of 'hummingbird' cells (>15 μm) was determined. RESULTS: Overall 72% (60/84) of the strains were found to harbor a functional cag PAI. Levels of phos-phorylated CagA were significantly higher for isolates from duodenal ulcer than the ones in strains from gas-tritis, atrophic gastritis, intestinal metaplasia and gastric cancer (49.1% ± 23.1% vs 21.1% ± 19.5%, P < 0.02; 49.1% ± 23.1% vs 26.2%±14.8%, P<0.045; 49.1% ± 23.1% vs 21.5% ± 19.5%, P<0.043 and 49.1% ± 23.1% vs 29.5% ± 27.1%, P < 0.047 respectively). We observed variable IL-8 expression levels ranging from 0 to 810 pg/mL and from 8.8 to 1442 pg/mL at 6 h and 30 h post-infection, respectively. cagPAI-defective strains did not induce detectable levels of IL-8 at 6 h post-infection. At 30 h post-infection all strains induced IL-8 expression in AGS cells, although cagPAI-defective strains induced significantly lower levels of IL-8 than strains with a functional cagPAI (57.1 ± 56.6 pg/mL vs 513.6 ± 338.6 pg/mL,P < 0.0001). We did not observe differences in the extent of cell elongation induction between strains with a functional or a defective cagPAI in 6 h cocultures. At 24 h post infection strains with functionalcagPAI showed high diversity in the extent of hummingbird phenotype induction ranging from 7% to 34%. cag PAI defective strains induced significantly lower levels of elongation than strains with functional cag-PAI with one or more than one EPIYA-C motif (15.1% ± 5.2%vs 18.9% ± 4.7%,P < 0.03; and 15.1% ± 5.2% vs 20.0% ± 5.1%, P < 0.003 respectively). No differences were observed in cellular elongation inductionor IL-8 expression among H. pylori strains bearing one and more than one EPIYA-C motifs, neither at 6 h nor at 24 h of coculture. There were no associations between the levels of induction of cell elongation or IL-8 expression and number of EPIYA motifs or pathology. CONCLUSION: The present work describes a lack of association between H. pylori CagA protein EPIYA motifs variations from Colombian isolates and disease-associated cellular responses. | Carlos Alberto Fajardo Andrés Javier Quiroga Andrea Coronado Karen Labrador Nicole Acosta Pilar Delgado Carlos Jaramillo María Mercedes Bravo | 2013 | World Journal of Gastrointestinal Oncology2013，5，3： | 3 |
| 18 | 组群随机试验在伤寒疫苗效果观察中的应用研究显示文摘目的 用组群随机试验实现伤寒Vi疫苗效果观察的研究设计和实施应用。方法 采用组群随机试验方法确定试验组和对照组所需样本量 ,并组织实施大规模疫苗接种 ,在试验组接种伤寒Vi疫苗、对照组接种流脑疫苗。结果 根据组群随机试验方法计算共需要观察对象为 96 12 1人 ,分为 10 8个组群。根据组群大小、地理位置 (城乡 )及性质 (学校、机关、厂矿、人口特征 )分层配对 ,确定试验组分为 5 3个组群共 4 4 0 5 4人 ,对照组为 5 4个组群共 4 84 2 2人。对两组间各主要混杂因子 (年龄、性别、居住地点、主要人群的经济收入、受教育程度、主要职业 )等进行分析 ,发现两组之间总体相差不大 ,具有较好的均衡性与可比性。结论 组群随机抽样法应用于大规模疫苗效果观察试验 ,能较好地控制组间混杂因子 ,使干预的实际效果得到科学的评价 ;实施也比较简单 ,被干预对象容易接受 ,可行性高。 | 董柏青 杨进 唐振柱 杨宏徽 曾竣 张杰 王鸣柳 梁贵臣 司国爱 李翠云 梁大斌 廖和壮 Rion Leo nOchiai Ali Mohammad Camilo J Acosta John Clemens | 2005 | 中华流行病学杂志2005，26，2： | 3 |
| 19 | Pathological correlations between traumatic brain injury and chronic neurodegenerative diseases显示文摘Traumatic brain injury is among the most common causes of death and disability in youth and young adults.In addition to the acute risk of morbidity with moderate to severe injuries,traumatic brain injury is associated with a number of chronic neurological and neuropsychiatric sequelae including neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease.However,despite the high incidence of traumatic brain injuries and the established clinical correlation with neurodegeneration,the causative factors linking these processes have not yet been fully elucidated.Apart from removal from activity,few,if any prophylactic treatments against post-traumatic brain injury neurodegeneration exist.Therefore,it is imperative to understand the pathophysiological mechanisms of traumatic brain injury and neurodegeneration in order to identify potential factors that initiate neurodegenerative processes.Oxidative stress,neuroinflammation,and glutamatergic excitotoxicity have previously been implicated in both secondary brain injury and neurodegeneration.In particular,reactive oxygen species appear to be key in mediating molecular insult in neuroinflammation and excitotoxicity.As such,it is likely that post injury oxidative stress is a key mechanism which links traumatic brain injury to increased risk of neurodegeneration.Consequently,reactive oxygen species and their subsequent byproducts may serve as novel fluid markers for identification and monitoring of cellular damage.Furthermore,these reactive species may further serve as a suitable therapeutic target to reduce the risk of post-injury neurodegeneration and provide long term quality of life improvements for those suffering from traumatic brain injury. | Marcela Cruz-Haces Jonathan Tang Glen Acosta Joseph Fernandez Riyi Shi | 2017 | Translational Neurodegeneration2017，6，1： | 2 |
| 20 | Zika Virus in an American Recreational Traveler显示文摘 | Dyan J. Summers Rebecca Wolfe Acosta Alberto M. Acosta | 2015 | J Travel Med2015，，5： | 2 |
您所在的机构暂未开通此部分的功能，请联系管理员