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| 1 | Maize Genic Male-Sterility Genes and Their Applications in Hybrid Breeding: Progress and Perspectives显示文摘As one of the most important crops, maize not only has been a source of the food, feed, and industrial feedstock for biofuel and bioproducts, but also became a model plant system for addressing fundamental questions in genetics. Male sterility is a very useful trait for hybrid vigor utilization and hybrid seed production. The identification and characterization of genic male-sterility (GMS) genes in maize and other plants have deepened our understanding of the molecular mechanisms controlling anther and pollen development, and enabled the development and efficient use of many biotechnology-based male-sterility (BMS) systems for crop hybrid breeding. In this review, we summarize main advances on the identification and characterization of GMS genes in maize, and con struct a putative regulatory network controlling maize anther and pollen development by comparative genomic analysis of GMS genes in maize, Arabidopsis, and rice. Furthermore, we discuss and appraise the features of more than a dozen BMS systems for propagating male-sterile lines and producing hybrid seeds in maize and other plants. Finally, we provide our perspectives on the studies of GMS genes and the development of novel BMS systems in maize and other plants. The continuous exploration of GMS genes and BMS systems will enhance our understanding of molecular regulatory networks controlling male fertility and greatly facilitate hybrid vigor utilization in breeding and field production of maize and other crops. | Xiangyuan Wan Suowei Wu Ziwen Li Zhenying Dong Xueli An Biao Ma Youhui Tian Jinping Li | 2019 | Molecular Plant2019，12，3： | 24 |
| 2 | Temperature variation of a thermionic cathode during electron emission显示文摘It is necessary to know the actual temperature of a thermionic cathode that works as the electron source in a microwave tube. It has been found that the temperature of the cathode drops markedly during the thermionic emission. For example, the temperature could fall by about 30oC under a current density of 2.92 A/cm2. Using the molecular thermodynamics, the dependence of the cathode temperature on the emission current density has been obtained. It has been theoretically pointed out that several factors, such as heating model and temperature coefficient of resis-tance of heater, can influence the cathode temperature. These theoretical conclu-sions were supported by the experimental results. | LIU YanWen TIAN Hong HAN Yong XU ZhenYing MENG MingFeng ZHANG HongLai | 2008 | Science China(Technological Sciences)2008，51，9： | 22 |
| 3 | Abscisic Acid Signaling Inhibits Bmssinosteroid Signaling through Dampening the Dephosphorylation of BIN2 by ABI1 and ABI2显示文摘 | Haijiao Wang Jie Tang Jing Liu Jin Hu Jingjing Liu Yuxiao Chen Zhenying Cai Xuelu Wang | 2018 | Molecular Plant2018，11，2： | 17 |
| 4 | ZmMs30 Encoding a Novel GDSL Lipase Is Essential for Male Fertility and Valuable for Hybrid Breeding in Maize显示文摘Genic male sterility (GMS) is very useful for hybrid vigor utilization and hybrid seed production. Although a large number of GMS genes have been identified in plants, little is known about the roles of GDSL lipase members in anther and pollen development. Here, we report a maize GMS gene, ZmMs30, which encodes a novel type of GDSL lipase with diverged catalytic residues. Enzyme kinetics and activity assays show that ZmMs30 has lipase activity and prefers to substrates with a short carbon chain. ZmMs30 is specifically expressed in maize anthers during stages 7-9. Loss of ZmMs30 function resulted in defective anther cuticle, irregular foot layer of pollen exine, and complete male sterility. Cytological and lipidomics analyses demonstrate that ZmMs30 is crucial for the aliphatic metabolic pathway required for pollen exine formation and anther cuticle development. Furthermore, we found that male sterility caused by loss of ZmMs30 function was stable in various inbred lines with different genetic background, and that it didn't show any negative effect on maize heterosis and production, suggesting that ZmMs30 is valuable for crossbreeding and hybrid seed production. We then developed a new multi-control sterility system using ZmMs30 and its mutant line, and demonstrated it is feasible for generating desirable GMS lines and valu. able for hybrid maize seed production. Taken together, our study sheds new light on the mechanisms of anther and pollen development, and provides a valuable male-sterility system for hybrid breeding maize. | Xueli An Zhenying Dong Youhui Tian Ke Xie Suowei Wu Taotao Zhu Danfeng Zhang Van Zhou Canfang Niu Biao Ma Quancan Hou Jianxi Bao Simiao Zhang Ziwen Li Yanbo Wang Tingwei Yan Xiaojing Sun Yuwen Zhang Jinping Li Xiangyuan Wan | 2019 | Molecular Plant2019，12，3： | 15 |
| 5 | Dynamic feedback robust stabilization of nonholonomic mobile robots based on visual servoing显示文摘This paper investigates the visual servoing robust stabilization of nonholonomic mobile robots. The calibration of visual parameters is not only complicated, but also needs great consumption of calculated time so that the accurate calibration is impossible in some situations for high requirement of real timing. Hence, it is interesting and important to consider the design of stabilizing controllers for nonholonomic kinematic systems with uncalibrated visual parameters. A novel uncertain model of these nonholonomic kinematic systems is proposed. Based on this model, a stabilizing controller is discussed by using dynamic feedback and two-step techniques. The proposed robust controller makes the mobile robot image pose and the orientation converge to the desired configuration despite the lack of depth information and the lack of precise visual parameters. The stability of the closed loop system is rigorously proved. The simulation is given to show the effectiveness of the presented controllers. | Chaoli WANG 1 , Zhenying LIANG 2 , 3 , Qingwei JIA 4 (1.Department of Control Science and Engineering, University of Shanghai for Science and Technology, Shanghai 200093, China 2.Business School, University of Shanghai for Science and Technology, Shanghai 200093, China 3.School of Science, Shandong University of Technology, Zibo Shandong 255049, China 4.Research & Development Centre, Hitachi Asia Ltd, Hitachi Tower, Singapore 049318) | 2010 | 控制理论与应用(英文版)2010，8，2： | 13 |
| 6 | Dissecting and Enhancing the Contributions of High-Molecular-Weight Glutenin Subunits to Dough Functionality and Bread Quality显示文摘Dear Editor, Seed storage proteins (SSPs) are frequently important determinants of crop quality traits (Shewry and Casey,1999).Dissecting and enhancing the genetic contributions of individual SSPs to their target traits are essential for effectively improving crop quality attributes.However,such a task is often difficult to accomplish,because SSPs are frequently expressed from multigene families and exhibit strong allelic variation.Consequently,detailed knowledge of the function of individual SSPs in crop quality trait is still limited.This scenario is well illustrated by high-molecular-weight glutenin subunits (HMWGSs),a complex family of SSPs that are involved in wheat enduse quality through affecting dough functionality (Békés,2012;Rasheed et al.,2014). | Yiwen Li Xuefi An Ran Yang Xiaomin Guo Guidong Yue Renchun Fan Bin Li Zhensheng Li Kunpu Zhang Zhenying Dong Luyan Zhang Jiankang Wang Xu Jia Hong-Qing Ling Aimin Zhang Xiangqi Zhang Daowen Wang | 2015 | Molecular Plant2015，8，2： | 9 |
| 7 | Numerical Modelling of Dissolving and Driving Exploitation of Potash Salt in the Qarhan Playa——A Coupled Model of Reactive Solute Transport and Chemical Equilibrium in a Multi-component Underground Brine System显示文摘第一,在在 Qarhan 盐湖的 intercrystal 盐水和它的媒介盐层(盐存款) 之间的一系列化学反应的宏观的化学平衡状态被使用 Pitzer 理论学习。有溶解比率的积累的矿石的宏观的溶解度产品和它的关系的概念被介绍,它在在 Qarhan 盐湖溶解并且开车钾盐的利用的数字模型被使用。并且第二,在多部件为反应溶质与形成运输的想法的一个模型一起当模特儿在多孔的媒介的新鲜地下水系统是一本参考书,一个二维的运输模型在多部件盐水结合了一系列化学反应多孔的系统(盐存款) 被使用 Pitzer 理论开发。同时,模型被使用在 Qarhan 盐湖为钾 / 镁运输建模以便在溶解并且开车的过程并且到为在盐的采矿溶解并且捕获最大的钾 / 镁的最佳的注射 / 抽象策略在 Qarhan 盐湖扔的设计学习固体和液体阶段的转移法律。 | LI Wenpeng LIU Zhenying | 2008 | Acta Geologica Sinica(English Edition)2008，82，5： | 9 |
| 8 | MiR156 regulates anthocyanin biosynthesis through SPL targets and other microRNAs in poplar显示文摘Anthocyanins biosynthesized from the flavonoid pathway are types of pigments that are involved in the protection of poplar from biotic and abiotic stresses.Previous researchers studying anthocyanin-related transcription factors and structural genes in poplar have made significant discoveries.However,little is known about the regulatory role of microRNAs in anthocyanin biosynthesis in poplar.Here,we overexpressed miR156 in poplar to study the comprehensive effects of the miR156-SPL module on the biosynthesis of anthocyanins.Small RNA sequencing analysis revealed 228 microRNAs differentially expressed in transgenic poplar plants with dramatically increased miR156 levels.Furthermore,integrated microRNAomic and transcriptomic analysis suggested that two microRNAs,miR160h,and miR858,have the potential to affect anthocyanin accumulation in poplar by regulating auxin response factors and MYB transcription factors,respectively.Additionally,the accumulation of miR160h and miR858 displayed a positive correlation with miR156 levels,suggesting a possible interaction between the miR156-SPL module and these microRNAs in poplar.Last,metabolomics analysis revealed that the levels of anthocyanins,flavones,and flavonols were substantially elevated in transgenic poplar plants overexpressing miR156 compared with the wild type,whereas the total lignin content was reduced in the transgenic plants.Taken together,our results indicate that miR156 can fine tune the anthocyanin biosynthetic pathway via multiple factors,including microRNAs,transcription factors,and the levels of structural genes,in poplar.This provides additional clues for understanding the complex regulatory network of anthocyanin biosynthesis in woody plants. | Yamei Wang Wenwen Liu Xinwei Wang Ruijuan Yang Zhenying Wu HanWang LeiWang Zhubing Hu Siyi Guo Hailing Zhang Jinxing Lin Chunxiang Fu | 2020 | Horticulture Research2020，7，1： | 9 |
| 9 | Normal Structure and Function of Endothecium Chloroplasts Maintained by ZmMs33-Mediated Lipid Biosynthesis in Tapetal Cells Are Critical for Anther Development in Maize显示文摘Genic male sterility(GMS)is critical for heterosis utilization and hybrid seed production.Although GMS mutants and genes have been studied extensively in plants,it has remained unclear whether chloroplast-associated photosynthetic and metabolic activities are involved in the regulation of anther development.In this study,we characterized the function of ZmMs33/ZmGPAT6,which encodes a member of the glycerol-3-phosphate acyltransferase(GPAT)family that catalyzes the first step of the glycerolipid synthetic pathway.We found that normal structure and function of endothecium(En)chloroplasts maintained by ZmMs33-mediated lipid biosynthesis in tapetal cells are crucial for maize anther development.ZmMs33 is expressed mainly in the tapetum at early anther developmental stages and critical for cell proliferation and expansion at late stages.Chloroplasts in En cells of wild-type anthers function as starch storage sites before stage 10 but as photosynthetic factories since stage 10 to enable starch metabolism and carbohydrate supply.Loss of ZmMs33 function inhibits the biosynthesis of glycolipids and phospholipids,which are major components of En chloroplast membranes,and disrupts the development and function of En chloroplasts,resulting in the formation of abnormal En chloroplasts containing numerous starch granules.Further analyses reveal that starch synthesis during the day and starch degradation at night are greatly suppressed in the mutant anthers,leading to carbon starvation and low energy status,as evidenced by low trehalose-6-phosphate content and a reduced ATP/AMP ratio.The energy sensor and inducer of autophagy,SnRK1,was activated to induce early and excessive autophagy,premature PCD,and metabolic reprogramming in tapetal cells,finally arresting the elongation and development of mutant anthers.Taken together,our results not only show that ZmMs33 is required for normal structure and function of En chloroplasts but also reveal that starch metabolism and photosynthetic activities of En chloroplasts at different developmental stages are essential for normal anther development.These findings provide novel insights for understanding how lipid biosynthesis in the tapetum,the structure and function of En chloroplasts,and energy and substance metabolism are coordinated to maintain maize anther development. | Taotao Zhu Ziwen Li Xueli An Yan Long Xiaofeng Xue Ke Xie Biao Ma Danfeng Zhang Yijian Guan Canfang Niu Zhenying Dong Quancan Hou Lina Zhao Suowei Wu Jinping Li Weiwei Jin Xiangyuan Wan | 2020 | Molecular Plant2020，13，11： | 9 |
| 10 | An Efficient and Stable Ionic Liquid System for Synthesis of Ethylene Glycol via Hydrolysis of Ethylene Carbonate显示文摘An ionic liquid system of [Bmim]X/[Bmim]OH(X Cl,BF4,and PF6,) was developed for the hydroly-sis of ethylene carbonate to ethylene glycol. The important parameters,such as the variety of ionic liquids,molar ratio of [Bmim]X to [Bmim]OH,amount of ionic liquid,molar ratio of water to ethylene carbonate,reaction tem-perature,pressure and reaction time,were investigated systematically. Excellent yield(>93%) and high selectivity(99.5%) of ethylene glycol were achieved. Under the optimum reaction conditions,the ionic liquid system could be reused at least five times and the selectivity of ethylene glycol remained higher than 99.5%. | MENG Zhenying SUN Jian WANG Jinquan ZHANG Jianxin FU Zengzeng CHENG Weiguo ZHANG Xiangping | 2010 | Chinese Journal of Chemical Engineering2010，18，6： | 8 |
| 11 | Water quality characteristics and assessment of Yongding New River by improved comprehensive water quality identification index based on game theory显示文摘The Yongding New River is essential for the water supplies of Tianjin.To date,there is no comprehensive report that assesses the year-round water quality of the Yongding New River Main stream.Moreover,little attention has been given to determining a combined weight for improving the traditional comprehensive water quality identification index(ICWQII)by the game theory.Seven water quality parameters were investigated monthly along the main stream of the Yongding New River from May 2018 to April 2019.Organic contaminants and nitrogen pollution were mainly caused by point sources pollution,and the total phosphorus mainly by non-point source pollution.Dramatic spatio-temporal variations of water quality parameters were jointly caused by different pollutant sources and hydrometeorological factors.In terms of this study,an improved comprehensive water quality identification index(ICWQII)based on entropy weight or variation coefficient and traditional CWQII underestimated the water qualities,and an ICWQII based on the superstandard multiple method overvalued the assessments.By contrast,water qualities assessments done with an ICWQII based on the game theory matched perfectly with the practical situation.The ICWQII based on game theory proposed in this study takes into account not only the degree of disorder and variation of water quality data,but also the influence of standard-exceeded pollution indicators,whose results are relatively reasonable.All findings and the ICWQII based on game theory can provide scientific support for decisions related to the water environment management of the Yongding New River and other waters. | Yu Liu Yucheng Hu Yumei Hu Yuqi Gao Zhenying Liu | 2021 | Journal of Environmental Sciences2021，33，6： | 8 |
| 12 | Amyloid fibril structure of α-synuclein determined by cryo- electron microscopy显示文摘 | Yaowang Li Chunyu Zhao Feng LUO Zhenying Liu Xinrui Gui Zhipu LUO Xiang Zhang Dan Li Cong Liu Xueming Li | 2018 | Cell Research2018，28，9： | 5 |
| 13 | The art of two-dimensional soft nanomaterials显示文摘The discovery of graphene has triggered the explosive development of two-dimensional(2D) nanomaterials, including both inorganic and organic species. Benefiting from the simple elemental composition, inorganic 2D nanomaterials were the center research in the past decade, which has long shadowed the research of 2D organic(or soft) nanomaterials. Although many kinds of2D soft nanomaterials have been successfully prepared, a unified definition for them is still impossible due to the complicate and quite different chemical structures between each other and even relying on totally different techniques to distinguish them. Since our first review on 2D soft nanomaterials in 2015, this field has moved forward with big success. In this review, we will focus on the development of 2D soft nanomaterials after 2015. In order to deliver better overview of this field, new and comprehensive classification is used in this review: 2D aromatic molecules, graphene and graphene nanoribbons, graphyne and graphdiyne,BxCyNznanosheets, 2D polymers, 2D supramolecules, crystalline 2D assemblies, 2D covalent organic frameworks, 2D metalorganic frameworks, sandwich-like 2D porous polymers, 2D polymer nanosheets, etc. The focus of this review lies on synthetic strategies and the challenges of characterization, definition and fundamental understanding. | Caini Zheng Jinhui Zhu Chongqing Yang Chenbao Lu Zhenying Chen Xiaodong Zhuang | 2019 | Science China Chemistry2019，62，9： | 5 |
| 14 | 药物流行病学研究中处理偏倚的方法显示文摘药物流行病学是在应对处理药源性疾病、药物不良反应过程中发展起来的一门医药学领域的综合应用性学科,是流行病学的分支之一。正确评价药物在人群中的利用及其效应主要取决于合理的研究设计和得当的实施和分析方法来控制系统误差。本文基于《中国药物流行病学研究方法学指南》,检索相关文献,并结合实例,对药物流行病学研究中处理偏倚的方法进行解读,系统理解处理偏倚的方法。当前国内药物流行病学研究对偏倚处理的方法仍需加强,需重点关注选择偏倚、错分偏倚、混杂偏倚、时间依赖性偏倚、系统评价,根据产生原理及过程,在试验设计阶段使用随机化、盲法、限制、匹配等方法消除或减少偏倚,系统评价时采用漏斗图、秩相关分析法、回归分析法、剪补法等评估发表偏倚,提高药物流行病学在医学中的价值,促进全民健康发展。 | 陈明珠 胥洋 张兴会 孙凤 王树月 张灵健 王俊彦 宗晓郁 李慧 周锦 潘发明 方世平 宫建 詹思延 Zhao Zhenying Zhu Xiaohong | 2021 | 药物流行病学杂志2021，30，7： | 4 |
| 15 | Molecular genetic and genomic analysis of wheat milling and end-use traits in China:Progress and perspectives显示文摘Wheat is the most widely cultivated staple food crop, and multiple types of food derivatives are processed and consumed globally. Wheat grain quality(WGQ) is central to food processing and nutritional value, and is a decisive factor for consumer acceptance and commercial value of wheat cultivars. Hence, improvement in WGQ traits is top priority for both conventional and molecular wheat breeding. In this review we will focus on two important WGQ traits, grain milling and end-use, and will summarize recent progress in China. Chinese scientists have invested substantial effort in molecular genetic and genomic analysis of these traits and their effects on end-use properties. The insights and resources generated have contributed to the understanding and improvement of these traits. As high-quality genomics information and powerful genome engineering tools are becoming available for wheat, more fundamental breakthroughs in dissecting the molecular and genomic basis of WGQ are expected. China will strive to make further significant contributions to the study and improvement of WGQ in the genomics era. | Daowen Wang Kunpu Zhang Lingli Dong Zhenying Dong Yiwen Li Abrar Hussain Huijie Zhai | 2018 | The Crop Journal2018，6，1： | 4 |
| 16 | Short-Term and Continuing Stresses Differentially Interplay with Multiple Hormones to Regulate Plant Survival and Growth显示文摘压力植物激素, abscisic 酸(骆驼毛的织物) ,在便于植物熬过并且成长 undera 很好起重要作用压力条件的宽范围。主要集中于对短期的骆驼毛的织物处理的植物回答的以前的基因表示研究,而是持续骆驼毛的织物处理和他们的差别的效果糟糕被学习。这里,我们为 1h 或 9 d 与骆驼毛的织物对待植物,并且我们的染色体宽的分析显示差别在二个条件下面调整了基因是 tremendouslydifferent。我们分析了由把他们已知的应答的基因的整个集合用作集成他们的生合成的反馈规定的 reportersand 表明变化的另外的荷尔蒙。我们发现了那,在短期的骆驼毛的织物处理下面,表明 outputsof 支持生长的荷尔蒙, brassinosteroids 和赤霉素,和关於生命的压力应答的荷尔蒙, jasmonic 酸, weresignificantly 禁止了,当表明产量的植物生长素和乙烯被支持时。然而,支撑了骆驼毛的织物处理 repressedcytokinin 并且赤霉素发信号,但是刺激了植物生长素发信号。用荷尔蒙相关的异种的几个集合,我们包括受体或抄写管理者,在相应神经质的发信号小径的 foundcandidates 是到骆驼毛的织物的必要 inresponding。我们的调查结果显示骆驼毛的织物依赖者的相互作用在植物在不同层次 areinvolved 与荷尔蒙强调信号在短暂应力下面幸存并且适应继续紧张的 environments.Key 词: | Cangjing Yang Jingjing Liu Xinran Dong Zhenying Cai Weidong Tian Xuelu Wang | 2014 | Molecular Plant2014，7，5： | 4 |
| 17 | Robust exponential stabilization of nonholonomic wheeled mobile robots with unknown visual parameters显示文摘The visual servoing stabilization of nonholonomic mobile robot with unknown camera parameters is investigated.A new kind of uncertain chained model of nonholonomic kinemetic system is obtained based on the visual feedback and the standard chained form of type (1,2) mobile robot.Then,a novel time-varying feedback controller is proposed for exponentially stabilizing the position and orientation of the robot using visual feedback and switching strategy when the camera parameters are not known.The exponential stability of the closed-loop system is rigorously proven.Simulation results demonstrate the effectiveness of the method proposed in this paper. | Liang, Zhenying Wang, Chaoli | 2011 | 控制理论与应用(英文版)2011，9，2： | 3 |
| 18 | A TiC_x reinforced Fe(Al) matrix composite using in-situ reaction显示文摘A new Fe matrix composite reinforced by the in-situ generated TiCx grains was prepared using the element Fe and Ti3AlC2 powders as the starting materials.Several hot-pressing temperatures were tried for exploring the phase transformation behavior from Ti3AlC2 to TiC x.Microstructures of the hot-pressed product were observed and analyzed.A tensile test was carried out for the new composite material,and the fracture face was analyzed.The results showed that the starting Ti3AlC2 was wholly decomposed and transformed into submicron TiC x grains at the hotpressing temperature above 1100℃.Most of the Al ions escaped from Ti3AlC2 were aggregated at the grain boundary of Fe grains,but a small amount of Al ions could be absorbed by Fe and formed Fe(Al) alloy around the surface of Fe grains.The prepared material exhibited a higher tensile strength of about 660 MPa and a uniform deformation of about 7%. | Xinhua Chen Hongxiang Zhai Wenjuan Wang Shibo Li Zhenying Huang | 2013 | Progress in Natural Science:Materials International2013，23，1： | 2 |
| 19 | Spatial-Temporal ConvLSTM for Vehicle Driving Intention Prediction显示文摘Driving intention prediction from a bird’s-eye view has always been an active research area. However,existing research, on one hand, has only focused on predicting lane change intention in highway scenarios and, on the other hand, has not modeled the influence and spatiotemporal relationship of surrounding vehicles. This study extends the application scenarios to urban road scenarios. A spatial-temporal convolutional long short-term memory(ConvLSTM) model is proposed to predict the vehicle’s lateral and longitudinal driving intentions simultaneously. This network includes two modules: the first module mines the information of the target vehicle using the long short-term memory(LSTM) network and the second module uses ConvLSTM to capture the spatial interactions and temporal evolution of surrounding vehicles simultaneously when modeling the influence of surrounding vehicles. The model is trained and verified on a real road dataset, and the results show that the spatial-temporal ConvLSTM model is superior to the traditional LSTM in terms of accuracy, precision, and recall, which helps improve the prediction accuracy at different time horizons. | He Huang Zheni Zeng Danya Yao Xin Pei Yi Zhang | 2022 | Tsinghua Science and Technology2022，27，3： | 2 |
| 20 | The behavior of 3d electrons and defects in TiAl-based alloys containing V and Cu studied by positron annihilation显示文摘Information of defects and 3d electrons in transition metals (Ti,V,Cu) and TiAl-based alloys (Ti50Al50,Ti50Al48V2,Ti50Al48Cu2) can be extracted from the positron lifetime and coincidence Doppler broadening spectra. The results show that the 3d electron signals for the transition metals Ti,V and Cu increase with the number of 3d electrons. The 3d electron signal and the electron density for binary TiAl alloy are relatively low due to the (Ti)3d-(Al)3p interactions. The addition of V and Cu atoms to TiAl alloy leads to the increase in the electron densities in bulk and the defects on grain boundaries simultaneously,as well as the enhancement of the 3d electron signal. The 3d electron signal in the spectrum of Ti50Al48Cu2 alloy is higher than that of Ti50Al48V2 alloy. | DENG Wen CHEN ZhenYing JIANG HaiFeng SUN ShunPing ZHU YingYing HUANG YuYang | 2008 | Science China(Physics,Mechanics & Astronomy)2008，51，9： | 2 |
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