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| 1 | Visceral hypersensitivity and altered colonic motility after subsidence of inflammation in a rat model of colitis显示文摘AIM: Irritable bowel syndrome (IBS) is a functional bowel disorder characterized by visceral hypersensitivity and altered bowel motility. There is increasing evidence suggesting the role of inflammation in the pathogenesis of IBS, which addresses the possibility that formerly established rat model of colitis could be used as an TBS model after the inflammation subsided.METHODS: Colitis was induced by intracolonic instillation of 4 % acetic acid in male Sprague-Dawley rats. The extent of inflammation was assessed by histological examination and myeloperoxidase (MPO) activity assay. After subsidence of colitis, the rats were subjected to rectal distension and restraint stress, then the abdominal withdrawal reflex and the number of stress-induced fecal output were measured,respectively.RESULTS: At 2 days post-induction of colitis, the colon showed characteristic inflammatory changes in histology and 8-fold increase in MPO activity. At 7 days post-induction of colitis, the histological features and MPO activity returned to normal. The rats at 7 days post-induction of colitis showed hypersensitive response to rectal distension without an accompaning change in rectal compliance, and defecated more stools than control animals when under stress.CONCLUSION: These results concur largely with the characteristic features of IBS, visceral hypersensitivity and altered defecation pattern in the absence of detectable disease, suggesting that this animal model is a methodologically convenient and useful model for studying a subset of IBS. | Jun-Ho La Tae-Wan Kim Tae-Sik Sung Jeoung-Woo Kang Hyun-Ju Kim ⅠI-Suk Yang | 2003 | World Journal of Gastroenterology2003，9，12： | 57 |
| 2 | p53 gene therapy in combination with transcatheter arterial chemoembolization for HCC:One-year follow-up显示文摘AIM:To evaluate the efficacy and safety of combination therapy with recombinant adenovirus p53 injection (rAdp53) and transcatheter hepatic arterial chemoembolization (TACE) for advanced hepatocellular carcinoma (HCC).METHODS:A total of 82 patients with advanced HCC treated only with TACE served as control group.Another 68 patients with HCC treated with TACE in combination with recombinant adenovirus-p53 injection served as p53 treatment group.Patients were followed up for 12 mo.Safety and therapeutic effects were evaluated according to the improvement in clinical symptoms,leukocyte count,Karnofsky and RECIST criteria.Survival rate was calculated with Kaplan-Meier method.RESULTS:The total effective rate was 58.3% for p53 treatment group,and 26.5% for control group (P < 0.05).The incidence of gastrointestinal symptoms was lower in p53 treatment group than in control group (P < 0.05).The 3-,6-and 12-mo survival rates were significantly higher for p53 treatment group than for control group (P < 0.01).The combination treatment was well tolerated with such adverse events as fever (51.5%,P=0.006) and pain of muscles and joints (13.2%,P=0.003),which were significantly higher than the chemotherapy.Except for these minor adverse effects,no severe vector-related complications were identified.With respect to the efficacy,patients in p53 treatment group had less gastrointerestinal symptoms (P=0.062),better improvement in tumor-related pain (P=0.003),less downgrade of leukocyte counts (P=0.003) and more upgrade of Karnofsky performance score (P=0.029) than those in control group.The total effective rate (CR + PR) for p53 treatment group and control group was 58.3% and 26.5%,respectively,with distributions of different effect in two groups (P=0.042).The survival rates were 89.71%,76.13%,and 43.30% for p53 treatment group,and 68.15%,36.98%,and 24.02% for control group,respectively,3,6 and 12 mo after treatment,suggesting that the survival rates are significantly higher for p53 treatment group than for control group (P=0.0002).CONCLUSION:The rAd-p53 gene therapy in combination with TACE is a safe and effective treatment modality for advanced HCC. | Yong-Song Guan Yuan Liu Qing He Xiao Li Lin Yang Ying Hu Zi La | 2011 | World Journal of Gastroenterology2011，17，16： | 21 |
| 3 | Adenovirus-mediated wild-type p53 gene transfer in combination with bronchial arterial infusion for treatment of advanced non-small-cell lung cancer,one year follow-up显示文摘Objective:In the present study,we have examined the safety and efficacy of recombinant adenovirus encoding human p53 tumor suppressor gene(rAd-p53) injection in patients with advanced non-small-cell lung cancer(NSCLC) in the combination with the therapy of bronchial arterial infusion(BAI).Methods:A total of 58 patients with advanced NSCLC were enrolled in a non-randomized,two-armed clinical trial.Of which,19 received a combination treatment of BAI and rAd-p53(the combo group),while the remaining 39 were treated with only BAI(the control group).Patients were followed up for 12 months,with safety and local response evaluated by the National Cancer Institute's Common Toxicity Criteria and response evaluation criteria in solid tumor(RECIST),respectively.Time to progression(TTP) and survival rates were also analyzed by Kaplan-Meier method.Results:In the combo group,19 patients received a total of 49 injections of rAd-p53 and 46 times of BAI,respectively,while 39 patients in the control group received a total of 113 times of BAI.The combination treatment was found to have less adverse events such as anorexia,nausea and emesis,pain,and leucopenia(P<0.05) but more arthralgia,fever,influenza-like symptom,and myalgia(P<0.05),compared with the control group.The overall response rates(complete response(CR)+partial response(PR)) were 47.3% and 38.4% for the combo group and the control group,respectively(P>0.05).Patients in the combo group had a longer TTP than those in the control group(a median 7.75 vs 5.5 months,P=0.018).However,the combination treatment did not lead to better survival,with survival rates at 3,6,and 12 months in the combo group being 94.74%,89.47%,and 52.63%,respectively,compared with 92.31%,69.23%,and 38.83% in the control group(P=0.224).Conclusion:Our results show that the combination of rAd-p53 and BAI was well tolerated in patients with NSCLC and may have improved the quality of life and delayed the disease progression.A further study to better determine the efficacy of this combination therapy is warranted. | Yong-song GUAN Yuan LIU Qing ZOU Qing HE Zi LA Lin YANG Ying HU | 2009 | Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)2009，10，5： | 20 |
| 4 | Peripheral corticotropin releasing hormone mediates post-inflammatory visceral hypersensitivity in rats显示文摘AIM:To investigate whether peripheral corticotropin releasing hormone (CRH), which is up-regulated in intestinal inflammation, mediates the post-inflammatory visceral hypersensitivity in a rat model of colitis. METHODS:We measured mucosal myeloperoxidase (MPO) activity as a marker of inflammation, plasma CRH level, and abdominal withdrawal reflex (AWR) to colorectal distension as a visceral nociceptive response at 2, 7 and 14 d after the induction of colitis with 4% acetic acid. RESULTS:Colonic inflammation, quantified by MPO activity, significantly increased on d 2 and subsided thereafter, which indicated a resolution of inflammation within 7 d. On the contrary, plasma CRH level and AWR score were increased on d 2, remained high on d 7, and returned to control level on d 14. Intraperitoneal injection of a CRH antagonist, astressin (30 μg/kg), significantly attenuated the post-inflammatory visceral hypersensitivity on d 7. Furthermore, intraperitoneal administration of CRH (3 and 10 μg/kg) mimicked the post-inflammatory visceral hypersensitivity in naive rats. CONCLUSION:These results suggest that increased peripheral CRH mediates the enhanced visceral nociception in rats recovered from experimental colitis. | Jun-Ho La Tae-Sik Sung Hyun-Ju Kim Tae-Wan Kim Tong Mook Kang Il-Suk Yang | 2008 | World Journal of Gastroenterology2008，14，5： | 15 |
| 5 | The interaction of versican with its binding partners显示文摘Versican belongs to the family of the large aggregating chondroitin sulfate proteoglycans located primarily within theextracellular matrix (ECM). Versican, like other members of its family, has unique N- and C-terminal globular regions,each with multiple motifs. A large glycosaminoglycan-binding region lies between them. This review will begin byoutlining these structures, in the context of ECM proteoglycans. The diverse binding partners afforded to versican byvirtue of its modular design will then be examined. These include ECM components, such as hyaluronan, type Icollagen, tenascin-R, fibulin-1, and -2, fibrillin-1, fibronectin, P- and L-selectins, and chemokines. Versican also binds tothe cell surface proteins CD44, integrin β1, epidermal growth factor receptor, and P-selectin glycoprotein ligand-1.These multiple interactors play important roles in cell behaviour, and the roles of versican in modulating such processesare discussed. | Yao Jiong WU David P. LA PIERRE Jin WU Albert J. YEE Burton B. YANG | 2005 | Cell Research2005，15，7： | 13 |
| 6 | p53 gene in treatment of hepatic carcinoma:Status quo显示文摘Hepatocellular carcinoma (HCC) is one of the 10 most common cancers worldwide. There is no ideal treatment for HCC yet and many researchers are trying to improve the effects of treatment by changing therapeutic strategies. As the majority of human cancers seem to exhibit either abnormal p53 gene or disrupted p53 gene activation pathways, intervention to restore wild-type p53 (wt-p53) activities is an attractive anti-cancer therapy including HCC. Abnormalities of p53 are also considered a predisposition factor for hepatocarcinogenesis. p53 is frequently mutated in HCC. Most HCCs have defects in the p53-mediated apoptotic pathway although they carry wt-p53. High expression of p53 in vivo may exert therapeutic effects on HCC in two aspects: (1) High expression of exogenous p53 protein induces apoptosis of tumor cells by inhibiting proliferation of cells through several biologic pathways and (2) Exogenous p53 renders HCC more sensitive to some chemotherapeutic agents. Several approaches have been designed for the treatment of HCC via the p53 pathway by restoring the tumor suppression function from inactivation, rescuing the mutated p53 gene from instability, or delivering therapeutic exogenous p53. Products with p53 status as the target have been studied extensively in vitro and in vivo . This review elaborates some therapeutic mechanisms and advances in using recombinant human adenovirus p53 and oncolytic virus products for the treatment of HCC. | Yong-Song Guan Zi La Lin Yang Qing He Ping Li | 2007 | World Journal of Gastroenterology2007，13，7： | 12 |
| 7 | Increase in neurokinin-1 receptor-mediated colonic motor response in a rat model of irritable bowel syndrome显示文摘AIM: Irritable bowel syndrome (IBS) is a functional bowel disorder. Its major symptom is bowel dysmotility, yet the mechanism of the symptom is poorly understood. Since the neurokinin-1 receptor (NK1R)-mediated signaling in the gut is important in the control of normal bowel motor function,we aimed to investigate whether the NK1R-mediated bowel motor function was altered in IBS, using a rat IBS model that was previously reported to show colonic dysmotility in response to restraint stress.METHODS: IBS symptoms were produced in male SpragueDawley rats by inducing colitis with acetic acid. Rats were left to recover from colitis for 6 d, and used for experiments 7 d post-induction of colitis. Motor activities of distal colon were recorded in vitro.RESULTS: The contractile sensitivity of isolated colon to a NK1R agonist [Sar9, Met(O2)11]-substance P (1-30 nmol/L)was higher in IBS rats than that in normal rats. After the enteric neurotransmission was blocked by tetrodotoxin (TTX, 1 μmol/L), the contractile sensitivity to the NK1R agonist was increased in normal colon but not in IBS rat colon. The NK1R agonist-induced contraction was not different between the two groups when the agonist was challenged to the TTX-treated colon or the isolated colonic myocytes. A nitric oxide synthase inhibitor Nω-nitro-L-arginine methyl ester (L-NAME, 100 μmol/L) augmented the NK1R agonist-induced contraction only in normal rat colon.CONCLUSION: These results suggest that the NK1R-meidated colonic motor response is increased in IBS rats, due to the decrease in the nitrergic inhibitory neural component. | Jun-Ho La Tae-Wan Kim Tae-Sik Sung Hyn-Ju Kim Jeom-Yong Kim Il-Suk Yang | 2005 | World Journal of Gastroenterology2005，11，2： | 7 |
| 8 | Food Frequency Questionnaire for Chinese Children Aged 12-17 Years: Validity and Reliability显示文摘Objective The primary objective of this study was to examine the validity and reliability of a semi-quantitative food frequency questionnaire(FFQ) among Chinese children aged 12-17 years. Methods A semi-quantitative 72-food item FFQ was developed for children aged 12-17 years. The reliability and validity of this FFQ were evaluated against 24-h dietary recalls(24 h DRs) to measure the consumption of foods and nutrients. We administered two FFQs and three DRs to children(N = 160) over a period of 1 month to evaluate the reliability and validity. Reliability was examined by quartile agreement and intraclass correlation coefficients(ICCs), and validity was examined by quartile agreement, Bland-Altman plots and correlation with DRs. Results For reliability, the ICCs between the two FFQs ranged from 0.21 to 0.76 for foods and nutrients, and the quartile agreement ranged from 70.0% to 95.0% in the same or adjacent quartiles. Spearman’s correlation coefficients of foods and nutrients between the second FFQ and the 24 h DRs ranged from-0.04 to 0.59. The Bland-Altman plots demonstrated good agreement across the range of intakes among nutrients. The quartile agreement ranged from 50.0% to 100.0%, with infrequent misclassification. Conclusion The FFQ assessment of dietary intakes demonstrated acceptable relative validity and high reproducibility for Chinese children aged 12-17 years. | LIU Dan JU La Hong YANG Zhen Yu ZHANG Qian GAO Jian Fen GONG Di Ping GUO Dan Dan LUO Shu Quan ZHAO Wen Hua | 2019 | Biomedical and Environmental Sciences2019，32，7： | 6 |
| 9 | Effect of trapping vascular endothelial growth factor-A in a murine model of dry eye with inflammatory neovascularization显示文摘·AIM: To evaluate whether trapping vascular endothelial growth factor-A(VEGF-A) would suppress angiogenesis and inflammation in dry eye corneas in a murine corneal suture model.·METHODS: We established two groups of animals, one with non-dry eyes and the other with induced dry eyes.In both groups, a corneal suture model was used to induce inflammation and neovascularization. Each of two groups was again divided into three subgroups according to the treatment; subgroup I(aflibercept),subgroup II(dexamethasone) and subgroup III(phosphate buffered saline, PBS). Corneas were harvested and immunohistochemical staining was performed to compare the extents of neovascularization and CD11b+ cell infiltration. Real-time polymerase chain reaction was performed to quantify the expression of inflammatory cytokines and VEGF-A in the corneas.·RESULTS: Trapping VEGF-A with aflibercept resulted in significantly decreased angiogenesis and inflammation compared with the dexamethasone and PBS treatments in the dry eye corneas(all P <0.05), but with no such effects in non-dry eyes. The anti-inflammatory and antiangiogenic effects of VEGF-A trapping were stronger than those of dexamethasone in both dry eye and non-dry eye corneas(all P <0.05). The levels of RNA expression of VEGF-A, TNF-alpha, and IL-6 in the aflibercept subgroup were significantly decreasedcompared with those in the PBS subgroup in the dry eye group.·CONCLUSION: Compared with non-dry eye corneas,dry eye corneas have greater amounts of inflammation and neovascularization and also have a more robust response to anti-inflammatory and anti-angiogenic agents after ocular surface surgery. Trapping VEGF-A is effective in decreasing both angiogenesis and inflammation in dry eye corneas after ocular surface surgery. | Jin Woo Kwon Jin A Choi Eun Young Shin Tae Yoon La Dong Hyun Jee Yeon Woong Chung Yang Kyung Cho | 2016 | International Journal of Ophthalmology(English edition)2016，9，11： | 5 |
| 10 | Anthocyanin profi les and color properties of red wines made from Vitis davidii and Vitis vinifera grapes显示文摘Spine grape(Vitis davidii Foex.)is an important wild grape species native to China.Fifteen red spine grape clones and three red Vitis vinifera grape varieties were used to evaluate the differences in the anthocyanin profiles and color properties of wines made from V.davidii and V.vinifera grapes.Among spine wines,‘Junzi#2’wine had the highest total phenolic and total anthocyanin,‘Xiangzhenzhu’wine had the highest total flavonoids,and‘Junzi#1’wine had the highest total tannin.The anthocyanin compositions of all of the spine wines were dominated by Mv-3,5-diglucoside.The total individual anthocyanin contents in spine wines,except‘Gaoshan#5’,‘Junzi#5055’,‘Junzi#5061’,and‘Junzi#5044’,were signifi cantly higher than in V.vinifera wines.Most of the spine wines had a stronger red intensity and a brighter chroma with a bluer hue than V.vinifera wines.Correlation analysis revealed that the color properties were closely related to the anthocyanin composition.These results suggest that wines made from spine grapes may be useful for the wine industry for their color properties and high individual anthocyanin contents. | Yanlun Ju La Yang Xiaofeng Yue Yunkui Li Rui He Shenglin Deng Xin Yang Yulin Fang | 2021 | Food Science and Human Wellness2021，10，3： | 5 |
| 11 | Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model显示文摘AIM: To compare the effects of the surgical insult of cataract surgery on corneal inflammatory infiltration,neovascularization(NV) and lymphangiogenesis(LY)between the dry eye and non-dry eye in murine cataract surgery models.·METHODS: We established two groups of animals, one with normal eyes(non-dry eye) and the second with induced dry eyes. In both groups, we used surgical insults to mimic human cataract surgery, which consisted of lens extraction, corneal incision and suture.After harvesting of corneas on the 9th postoperative day and immunohistochemical staining, we compared NV, LY and CD11b+ cell infiltration in the corneas.· RESULTS: Dry eye group had significantly more inflammatory infiltration(21.75%±7.17% vs 3.65%±1.49%;P =0.049). The dry eye group showed significantly more NV(48.21% ±4.02% vs 26.24% ±6.01%; P =0.016) and greater levels of LY(9.27%±0.48% vs 4.84%±1.15%; P =0.007). In corneas on which no surgery was performed,there was no induction of NV in both the dry and non-dry group, but dry eye group demonstrated more CD11b+cells infiltration than the non-dry eye group(0.360% ±0.160% vs 0.023% ±0.006%; P =0.068). Dry eye group showed more NV than non-dry eye group in both topical PBS application and subconjunctival PBS injection(P =0.020 and 0.000, respectively).·CONCLUSION: In a murine cataract surgery model,preexisting dry eye can induce more postoperative NV,LY, and inflammation in corneal tissue. | Jin Woo Kwon Yeon Woong Chung Jin A Choi Tae Yoon La Dong Hyun Jee Yang Kyung Cho | 2016 | International Journal of Ophthalmology(English edition)2016，9，2： | 5 |
| 12 | The Tibetan Plateau Surface–Atmosphere Coupling System and Its Weather and Climate Effects: The Third Tibetan Plateau Atmospheric Science Experiment显示文摘The Tibetan Plateau(TP) is a key area affecting forecasts of weather and climate in China and occurrences of extreme weather and climate events over the world. The China Meteorological Administration, the National Natural Science Foundation of China, and the Chinese Academy of Sciences jointly initiated the Third Tibetan Plateau Atmospheric Science Experiment(TIPEX-Ⅲ) in 2013, with an 8–10-yr implementation plan. Since its preliminary field measurements conducted in 2013, routine automatic sounding systems have been deployed at Shiquanhe, Gaize, and Shenzha stations in western TP, where no routine sounding observations were available previously. The observational networks for soil temperature and soil moisture in the central and western TP have also been established. Meanwhile, the plateau-scale and regional-scale boundary layer observations, cloud–precipitation microphysical observations with multiple radars and aircraft campaigns, and tropospheric–stratospheric air composition observations at multiple sites, were performed. The results so far show that the turbulent heat exchange coefficient and sensible heat flux are remarkably lower than the earlier estimations at grassland, meadow, and bare soil surfaces of the central and western TP. Climatologically, cumulus clouds over the main body of the TP might develop locally instead of originating from the cumulus clouds that propagate northward from South Asia. The TIPEX-Ⅲ observations up to now also reveal diurnal variations, macro-and microphysical characteristics, and water-phase transition mechanisms, of cumulus clouds at Naqu station. Moreover, TIPEX-Ⅲ related studies have proposed a maintenance mechanism responsible for the Asian 'atmospheric water tower' and demonstrated the effects of the TP heating anomalies on African, Asian, and North American climates. Additionally, numerical modeling studies show that the Γ distribution of raindrop size is more suitable for depicting the TP raindrop characteristics compared to the M–P distribution, the overestimation of sensible heat flux can be reduced via modifying the heat transfer parameterization over the TP, and considering climatic signals in some key areas of the TP can improve the skill for rainfall forecast in the central and eastern parts of China. Furthermore, the TIPEX-Ⅲ has been promoting the technology in processing surface observations, soundings, and radar observations, improving the quality of satellite retrieved soil moisture and atmospheric water vapor content products as well as high-resolution gauge–radar–satellite merged rainfall products, and facilitating the meteorological monitoring, forecasting, and data sharing operations. | Ping ZHAO Yueqing LI Xueliang GUO Xiangde XU Yimin LIU Shihao TANG Wenming XIAO Chunxiang SHI Yaoming MA Xing YU Huizhi LIU La JIA Yun CHEN Yanju LIU Jian LI Dabiao LUO Yunchang CAO Xiangdong ZHENG Junming CHEN An XIAO Fang YUAN Donghui CHEN Yang PANG Zhiqun HU Shengjun ZHANG Lixin DONG Juyang HU Shuai HAN Xiuji ZHOU | 2019 | Journal of Meteorological Research2019，33，3： | 4 |
| 13 | Synergistic effect and different toxicities of adjuvant components of Realgar–Indigo Naturalis formula显示文摘Objective:Realgar–Indigo Naturalis formula(RIF)is a well-known arsenic-containing preparation that is used to treat acute promyelocytic leukemia(APL)in China.In recent multicenter clinical trials,complete remission rates in APL patients have ranged from 96.08%to 100%.RIF has a satisfactory therapeutic effect,but its safety is a widespread concern,since the preparation contains arsenic,a wide-ranging and naturally occurring toxicant.In this study,in order to determine the toxic potential of RIF,acute toxicity and sub chronic toxicity assays were performed to evaluate the toxic potential of Realgar and the adjuvant components of RIF in addition to Realgar’s synergy with these adjuvant components.Methods:KM mice and Wistar rats were selected for these experiments.To evaluate acute toxicity,the toxic effects of a single dose of a gradient of concentrations of Realgar were firstly determined.Then,the toxic effects of combinations of gradient doses of Realgar and fixed doses of Indigo naturalis and Salvia miltiorrhiza were evaluated.Results:The results showed that when Realgar was used alone,the2756.73 mg/kg(equivalent to 23.6 mg/kg).However,theto 936.90 mg/kg when Realgar was used with I.naturalis.By contrast,theto 7538.86 mg/kg when Realgar was used with S.miltiorrhiza.Hence,I.naturalis strengthened the toxicity of Realgar,whereas S.miltiorrhiza displayed the opposite effect.The sub chronic toxicity assessment results revealed a trend that was consistent with acute toxicity.Changes in the levels of different valence states of arsenic were also taken into account.The test results of the effects of in vitro combinations of Realgar and adjuvant components on soluble arsenic dissolution showed that I.naturalis increased the level of soluble arsenic in Realgar extracts and I.naturalis suspensions when the Realgar/I.naturalis ratio was 2,1.5,and 1.0.However,S.miltiorrhiza did not affect it.Conclusion:Based on the collective experimental results presented here,it can be concluded that the toxicity of RIF is the result of the soluble arsenic in Realgar and that the I.naturalis and S.miltiorrhiza in the RIF exert completely opposite effects on the toxicity of Realgar.This maybe an intelligent explanation for the compatibility of this formula,and this RIF study may therefore be viewed as a classic case of traditional Chinese medicine research on compatibility. | Huan-Hua Xu Zeng-Chun Ma Qiao-Li Shi Shi-Han Yang La Jiang Xiang-Mei Chen Yue Gao | 2018 | Chinese Herbal Medicines2018，10，2： | 3 |
| 14 | Clinical Effects of Pulse High‐Volume Hemofiltration on Severe Acute Pancreatitis Complicated With Multiple Organ Dysfunction Syndrome显示文摘 | La‐Ping Chu Jun‐Jing Zhou Ya‐Fen Yu Yang Huang Wen‐Xia Dong | 2012 | Therapeutic Apheresis and Dialysis2012，，1： | 2 |
| 15 | 益生菌抑制自发性高血压大鼠的菌群失调和血压升高:短链脂肪酸的作用显示文摘该研究旨在评估益生菌短双歧杆菌CECT7263(BFM)及发酵乳酸杆菌CECT5716(LC40)、短链脂肪酸丁酸盐及醋酸盐对自发性高血压大鼠(spontaneously hypertensive rat,SHR)心血管的作用。方法和结果:5周龄的Wistar Kyoto大鼠(WKY)10只和周龄匹配的SHR 50只随机分为6组:对照WKY、对照SHR、BFM干预的SHR、LC40干预的SHR、丁酸盐干预的SHR和醋酸盐干预的SHR。 | 袁源(译) 练桂丽(审校) Robles-Vera I Toral M de la Visitación N Sánchez M Gómez-Guzmán M Romero M Yang T Izquierdo-Garcia JL Jiménez R Ruiz-Cabello J Guerra-Hernández E Raizada MK Pérez-Vizcaíno F Duarte J | 2020 | 中华高血压杂志2020，28，9： | 2 |
| 16 | Human iPS Cells Loaded with MnO2-Based Nanoprobes for Photodynamic and Simultaneous Enhanced Immunotherapy Against Cancer显示文摘How to trigger strong anti-tumor immune responses has become a focus for tumor therapy.Here,we report the human-induced pluripotent stem cells(iPSs)to deliver MnO2@Ce6 nanoprobes into tumors for simultaneous photodynamic therapy(PDT)and enhanced immunotherapy.Ce6 photosensitizer was attached on manganese dioxide(MnO2)nanoparticles,and resultant MnO2@Ce6 nanoprobes were delivered into mitomycin-treated iPSs to form iPS-MnO2@Ce6 nanoprobes.The iPS-MnO2@Ce6 actively targeted in vivo tumors,the acidic microenvironment triggered interaction between MnO2 and H2O2,released large quantities of oxygen,alleviated hypoxia in tumor.Upon PDT,singlet oxygen formed,broken iPSs released tumor-shared antigens,which evoked an intensive innate and adaptive immune response against the tumor,improving dendritic cells matured,effector T cells,and natural killer cells were activated.Meanwhile,regulatory T cells were reduced,and then the immune response induced by iPS-MnO2@Ce6 was markedly stronger than the immune reaction induced by MnO2@Ce6(P<0.05).The iPS-MnO2@Ce6 markedly inhibited tumor growth and metastasis and reduced mortality in mice models with tumor.Human iPS s loaded with MnO2-based nanoprobes are a promising strategy for simultaneous PDT and enhanced immunotherapy against tumor and own clinical translational prospect. | Yanlei Liu Jingxing Yang Bin Liu Wen Cao Jingpu Zhang Yuming Yang Lijun Ma Jesus Martinez de la Fuente Jie Song Jian Ni Chunfu Zhang Daxiang Cui | 2020 | Nano-Micro Letters2020，12，10： | 2 |
| 17 | Protein-only' or 'virino' in prion diseases?显示文摘60-year prion and scrapie research has led to a dilemma in understanding the unknown aetiology of the infectious neurodegenerative disorders with intriguing features. Current progress and dilemma in prion research are briefly but critically reviewed. Instead of providing a comprehensive coverage of the research history, attentions in this view are drawn toward both the major breakthrough in the advancement of protein-only hypothesis, and the puzzle why this hypothesis has not been fully accepted. In order to resolve the prion enigma in neuroscience, it is suggested that both technical and concept barriers remain to be crossed. Since prion research is a multi-interdisciplinary subject, this view is intended to both facilitate a bette r understanding of prion phenomenon by more scientists in natural science, and invite scientists outside the fields of molecular genetics and protein science for collaboration. | YANG Chiming (Chi Ming Yang) & CHEN Yi1. Institute for Life Science and Health at UCSD, P.O.Box 12035, La Jolla, CA 92039, USA 2. Institute of Chemistry, Academy of Sciences, Beijing 100080, China Correspondence should be addressed to Yang Chiming | 2000 | Chinese Science Bulletin2000，45，3： | 2 |
| 18 | A local spatial decision support system for developing countries based on MCA,fuzzy sets and OWA–case study of a municipality in Cuba显示文摘This paper presents a Spatial Decision Support System for local governments of developing countries.It allows municipality government,enterprises,scientific community and civil society to address decision problems using GIS.The framework is supported by four modules of information technologies:Environmental Decision Support Database,Data Manipulation,Decision Support,and Mapping.A case study is presented covering the implementation of this framework in one municipality of Cuba.An example of land suitability planning for coconut crops is used to evaluate the system performance and usability.Results show local municipalities are able to use this framework to solve local decision problems using state of the art decision making even with low infrastructure development. | Ricardo DELGADO TELLEZ ZHONG Ershun ZUHU Yang Arisleydis PEÑA DE LA CRUZ | 2013 | Geo-Spatial Information Science2013，16，2： | 2 |
| 19 | Correlation of DNA methylation patterns to the phenotypic features of Tibetan elite alpinists in extreme hypoxia显示文摘High altitude is an extreme environment that imposes hypoxic pressure on physiological processes,and natives living at high altitudes are more adaptive in certain physiological processes.So far,epigenetic modifications under extreme changes in hypoxic pressures are relatively less understood.Here,we recruit 32 Tibetan elite alpinists(TEAs),who have successfully mounted Everest(8848 m)at least five times.Blood samples and physiological phenotypes of TEAs and 32 matched non-alpinist Tibetan volunteers(non-TEAs)are collected for analysis.Genome-wide DNA methylation analysis identifies 23,202 differentially methylated CpGs(P_(adj)<0.05,|β|>0.1)between the two groups.Some differentially methylated CpGs are in hypoxia-related genes such as PPP1R13L,MAP3K7CL,SEPTI-9,and CUL2.In addition,Gene ontology enrichment analysis reveals several inflammation-related pathways.Phenotypic analysis indicates that 12 phenotypes are significantly different between the two groups.In particular,TEAs exhibit higher blood oxygen saturation levels and lower neutrophil count,platelet count,and heart rate.For DNA methylation association analysis,we find that two CpGs(cg16687447,cg06947206)upstream of PTEN were associated with platelet count.In conclusion,extreme hypoxia exposure leads to epigenetic modifications and phenotypic alterations of TEA,providing us clues for exploring the molecular mechanism underlying changes under extreme hypoxia conditions. | Zhuoma Basang Shixuan Zhang La Yang Deji Quzong Yi Li Yanyun Ma Meng Hao WeiLin Pu Xiaoyu Liu Hongjun Xie Meng Liang Jiucun Wang Qiangba Danzeng | 2021 | Journal of Genetics and Genomics2021，48，10： | 2 |
| 20 | The effect of thermal chang on various dowel and core restorative materials显示文摘 | Yang HS Lang LA Guckes AD | 2001 | J Prosthet Dent2001，86，： | 1 |
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